##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465603
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597766766966707	31.0	31.0	34.0	30.0	34.0
2	31.803862947618466	33.0	31.0	34.0	30.0	34.0
3	31.972828783319695	33.0	31.0	34.0	30.0	34.0
4	35.66123714838607	37.0	35.0	37.0	33.0	37.0
5	35.427136423090055	37.0	35.0	37.0	33.0	37.0
6	35.43921967856736	37.0	35.0	37.0	33.0	37.0
7	35.47274394709656	37.0	35.0	37.0	33.0	37.0
8	35.54848658621186	37.0	35.0	37.0	35.0	37.0
9	37.218705635487744	39.0	37.0	39.0	34.0	39.0
10	36.81450506117873	39.0	37.0	39.0	32.0	39.0
11	36.874502526830796	39.0	37.0	39.0	33.0	39.0
12	36.791977285369725	39.0	35.0	39.0	33.0	39.0
13	36.729475540320834	39.0	35.0	39.0	33.0	39.0
14	37.838297863201056	40.0	37.0	41.0	33.0	41.0
15	37.87101887230108	40.0	37.0	41.0	33.0	41.0
16	37.7549843965782	40.0	36.0	41.0	33.0	41.0
17	37.71447993247466	39.0	36.0	41.0	33.0	41.0
18	37.61370094264856	39.0	36.0	41.0	33.0	41.0
19	37.62910462346677	39.0	36.0	41.0	32.0	41.0
20	37.5631965429776	39.0	36.0	41.0	32.0	41.0
21	37.52764479610312	39.0	36.0	41.0	32.0	41.0
22	37.4879500346862	39.0	36.0	41.0	32.0	41.0
23	37.33294244238117	39.0	36.0	41.0	32.0	41.0
24	37.18906450345037	39.0	35.0	41.0	32.0	41.0
25	37.2076618922129	39.0	35.0	41.0	32.0	41.0
26	37.14566272124535	39.0	35.0	41.0	32.0	41.0
27	37.09615917423213	39.0	35.0	41.0	31.0	41.0
28	36.95706213233162	39.0	35.0	40.0	31.0	41.0
29	36.833392396526655	39.0	35.0	40.0	31.0	41.0
30	36.85651724752633	39.0	35.0	40.0	31.0	41.0
31	36.79983805946267	39.0	35.0	40.0	31.0	41.0
32	36.73946258937335	39.0	35.0	40.0	31.0	41.0
33	36.6924697650144	39.0	35.0	40.0	31.0	41.0
34	36.62070261574775	39.0	35.0	40.0	30.0	41.0
35	36.49018799277496	39.0	35.0	40.0	30.0	41.0
36	36.477627936246115	39.0	35.0	40.0	30.0	41.0
37	36.37776603673086	39.0	35.0	40.0	30.0	41.0
38	36.37060542994783	39.0	35.0	40.0	30.0	41.0
39	36.30918829990357	39.0	35.0	40.0	30.0	41.0
40	36.14067778772903	38.0	35.0	40.0	30.0	41.0
41	36.075351748163136	38.0	35.0	40.0	30.0	41.0
42	35.962014849560674	38.0	35.0	40.0	30.0	41.0
43	35.906770360156614	38.0	35.0	40.0	29.0	41.0
44	35.86603608653724	38.0	35.0	40.0	29.0	41.0
45	35.69233875211285	38.0	35.0	40.0	29.0	41.0
46	35.55813429037184	38.0	35.0	40.0	28.0	41.0
47	35.409529148222845	38.0	34.0	40.0	28.0	41.0
48	35.340844023771325	38.0	34.0	40.0	28.0	41.0
49	35.16235934905918	37.0	34.0	40.0	27.0	41.0
50	35.11411008949685	37.0	34.0	40.0	27.0	41.0
51	33.7288591353578	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	1.0
12	3.0
13	9.0
14	13.0
15	19.0
16	54.0
17	134.0
18	231.0
19	437.0
20	795.0
21	1237.0
22	1776.0
23	2220.0
24	2727.0
25	3056.0
26	3498.0
27	4243.0
28	5317.0
29	6847.0
30	8753.0
31	11799.0
32	15281.0
33	20820.0
34	31524.0
35	40423.0
36	46070.0
37	65598.0
38	95205.0
39	97490.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.476463854399565	35.52167833970142	20.295831427203005	24.706026378696013
2	20.777142758959886	34.60351415261499	19.52672126253482	25.092621825890298
3	20.623793231572822	31.41710856674034	21.901920735046808	26.057177466640034
4	22.558273894283325	30.687087497288463	19.496223177256162	27.25841543117205
5	16.528888344791593	36.3006681657979	18.084720244500144	29.085723244910362
6	20.140334147331526	37.4606692826292	21.145482310036662	21.25351426000262
7	76.75186800772332	9.304493312972639	8.114638436608011	5.829000242696031
8	78.10087134318293	6.0070489236538425	6.83114155192299	9.06093818124024
9	70.81827221903639	10.081979712330032	12.179689563855902	6.920058504777675
10	32.212636086966796	32.298331411094864	15.029112784926212	20.45991971701213
11	24.79515810679914	27.24574369151401	27.389428332721224	20.56966986896562
12	25.962461582077434	21.60681954368851	32.47380278907138	19.956916085162682
13	24.925526682602992	24.70559682819913	28.06231918608772	22.30655730311016
14	18.802713900039304	26.574571040135055	31.952113710607534	22.67060134921811
15	18.084505469251702	29.93644800398623	29.15466609966001	22.82438042710206
16	20.059149103420726	27.017222827172503	27.679804468613817	25.243823600792954
17	18.330208353468514	27.265717789619053	29.34431264403365	25.059761212878783
18	19.569891087471518	26.675944957399327	27.279678180767736	26.474485774361415
19	19.94488867124997	31.31981537919644	28.33486897635969	20.400426973193902
20	21.693159193561897	29.00432342575112	26.009497361486073	23.29302001920091
21	21.792170583093323	28.802864242713213	25.364097739920062	24.040867434273405
22	19.91310193448066	27.438182314117395	26.205372388064507	26.443343363337434
23	21.993844541379673	27.12461045139314	23.25972985569251	27.62181515153468
24	18.65194167563353	31.023210761099048	24.540219886899354	25.784627676368068
25	21.815151534676538	27.907681007210005	23.905559027755405	26.37160843035805
26	22.938855634521254	28.078856880217696	25.141161032038024	23.841126453223023
27	20.200041666398196	26.542354752868864	26.155329755177693	27.102273825555244
28	21.735255142256385	27.704288846936127	26.498540602186843	24.06191540862065
29	21.254588136244827	29.18666761167776	24.981368247197718	24.577376004879696
30	24.12484455641394	27.037841251022865	24.641593804163634	24.195720388399558
31	24.193572635915146	26.75562657457104	21.508452479902406	27.54234830961141
32	22.16523518963581	25.350781674516703	24.76122361754542	27.72275951830207
33	22.47343767114903	24.851858772387637	27.46202236669437	25.212681189768965
34	21.6869307113571	24.997905941327698	25.726208808792038	27.588954538523165
35	21.96420555709478	24.027766144118488	30.208568243761313	23.799460055025417
36	25.61345180336037	23.93133205756836	28.388562788470008	22.066653350601264
37	23.312779342057503	25.584457144820803	24.391380639729555	26.711382873392136
38	22.846931828188392	23.91114318421488	27.786762542337573	25.455162445259162
39	22.681340111640175	21.120783156465915	28.637487301413433	27.560389430480477
40	24.475142986621652	24.137516296071976	27.75862698479176	23.628713732514612
41	21.9476678629648	21.029288900629936	32.280719840722675	24.74232339568259
42	23.488680270530903	24.51229910460199	26.93754121000079	25.061479414866312
43	21.35682115450287	22.136025755847793	29.243583052514694	27.263570037134638
44	24.14718118225183	20.252446827017867	26.00563140701413	29.594740583716174
45	25.93475557502851	22.061713519887114	27.38040777228669	24.623123132797684
46	20.996643062866863	21.81772883765783	31.931924837254055	25.25370326222125
47	23.10079617184597	22.918881536416215	28.108495864502594	25.871826427235217
48	22.61304158263585	20.393983715740664	32.47294368807761	24.520031013545875
49	22.106171996314455	20.56623346499056	30.565095156173822	26.76249938252116
50	20.944667452744074	20.131743137393872	28.332291673378396	30.591297736483657
51	21.743201826448715	19.332993988440798	33.14218336222061	25.781620822889888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2820.0
1	2264.0
2	1708.0
3	1165.0
4	622.0
5	597.5
6	573.0
7	608.5
8	644.0
9	668.0
10	692.0
11	746.5
12	801.0
13	829.0
14	857.0
15	971.5
16	1086.0
17	1162.0
18	1238.0
19	1282.0
20	1326.0
21	1343.5
22	1361.0
23	1998.0
24	2635.0
25	2395.0
26	2842.0
27	3529.0
28	4006.5
29	4484.0
30	5243.0
31	6002.0
32	7083.0
33	8164.0
34	8482.0
35	8800.0
36	9897.0
37	10994.0
38	11379.5
39	11765.0
40	12332.0
41	12899.0
42	16795.0
43	20691.0
44	29074.5
45	37458.0
46	41410.0
47	45362.0
48	49868.0
49	54374.0
50	50175.0
51	45976.0
52	40388.5
53	34801.0
54	30810.0
55	26819.0
56	24739.5
57	22660.0
58	20808.5
59	18957.0
60	18139.5
61	17322.0
62	16234.5
63	15147.0
64	13198.0
65	11249.0
66	10105.0
67	8961.0
68	7789.5
69	6618.0
70	5771.0
71	4924.0
72	4114.0
73	3304.0
74	2589.0
75	2065.5
76	2257.0
77	1604.5
78	952.0
79	628.5
80	305.0
81	251.5
82	198.0
83	185.0
84	172.0
85	107.0
86	42.0
87	27.5
88	13.0
89	7.0
90	1.0
91	3.5
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.613736217599243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89735433108268	20.681957858945815
2	10.722770812119013	5.921915294552537
3	4.280024034175145	3.545623640540923
4	2.1357894119651313	2.3590850175338614
5	1.2534744233353257	1.7306556040744505
6	0.7983990889706626	1.3228069103524456
7	0.6034813917357797	1.1665063174535049
8	0.4103596840788853	0.906525125639336
9	0.34000331156137925	0.8449885582709524
>10	3.105332742983229	20.389162827273204
>50	1.1247350700062377	22.097897232878832
>100	0.31420673950139516	12.650525472586091
>500	0.008597696852028224	1.6750674456646248
>1k	0.004689652828379032	3.4038769083335656
>5k	7.816088047298386E-4	1.3034057858998414
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	6039	1.2970277253368212	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	3660	0.7860774092950431	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGT	3545	0.7613782557242974	No Hit
AATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	3005	0.6453996215660123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2410	0.5176083487434574	No Hit
AAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	1871	0.4018444898336136	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	1280	0.27491231800482385	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	951	0.20425126126764648	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	816	0.1752566027280752	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	752	0.16151098682783402	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	730	0.15678593136212612	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	691	0.14840969667291662	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	690	0.14819492142447538	No Hit
AACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	674	0.14475851744941506	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	643	0.13810048474773573	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	624	0.1340197550273516	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTAT	621	0.1333754292820278	No Hit
ACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCG	569	0.12220711636308185	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	498	0.10695807372375178	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCCTGTTC	494	0.10609897272998671	No Hit
AACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	484	0.10395122024557402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4132211347435477	0.0
2	0.0	0.0	0.0	1.8902369615316053	0.0
3	0.0	0.0	0.0	2.580739385270284	0.0
4	0.0	0.0	0.0	5.590384941677781	0.0
5	0.0	0.0	0.0	6.0160694840883755	0.0
6	0.0	0.0	0.0	8.08736198005597	0.0
7	2.1477524844126865E-4	0.0	0.0	9.57210327253046	0.0
8	2.1477524844126865E-4	0.0	0.0	10.704827932809712	0.0
9	2.1477524844126865E-4	0.0	0.0	12.882004626258851	0.0
10	2.1477524844126865E-4	0.0	0.0	15.280829376099382	0.0
11	2.1477524844126865E-4	0.0	0.0	17.911396619008038	0.0
12	2.1477524844126865E-4	0.0	0.0	18.933297251091595	0.0
13	2.1477524844126865E-4	0.0	0.0	19.37251263415399	0.0
14	2.1477524844126865E-4	0.0	0.0	19.931787381095052	0.0
15	2.1477524844126865E-4	0.0	0.0	20.4472479773541	0.0
16	2.1477524844126865E-4	0.0	0.0	21.262749595685595	0.0
17	2.1477524844126865E-4	0.0	0.0	22.361539766711125	0.0
18	2.1477524844126865E-4	0.0	0.0	23.93176160806524	0.0
19	2.1477524844126865E-4	0.0	0.0	24.54988477307921	0.0
20	2.1477524844126865E-4	0.0	0.0	25.26207949691046	0.0
21	2.1477524844126865E-4	0.0	0.0	25.85528873310524	0.0
22	2.1477524844126865E-4	0.0	0.0	26.4613844842065	0.0
23	2.1477524844126865E-4	0.0	0.0	27.065762033320233	0.0
24	2.1477524844126865E-4	0.0	0.0	27.504762641134185	0.0
25	2.1477524844126865E-4	0.0	0.0	27.917775443886743	0.0
26	2.1477524844126865E-4	0.0	0.0	28.28439679297599	0.0
27	2.1477524844126865E-4	0.0	0.0	28.73628391569642	0.0
28	2.1477524844126865E-4	0.0	0.0	29.12717486785953	0.0
29	2.1477524844126865E-4	0.0	0.0	29.538254693376118	0.0
30	2.1477524844126865E-4	0.0	0.0	30.090012306621734	0.0
31	2.1477524844126865E-4	0.0	0.0	30.437518658599707	0.0
32	2.1477524844126865E-4	0.0	0.0	30.820248151322048	0.0
33	2.1477524844126865E-4	0.0	0.0	31.229180224354224	0.0
34	2.1477524844126865E-4	0.0	0.0	31.580767306052582	0.0
35	2.1477524844126865E-4	0.0	0.0	31.99657218703488	0.0
36	2.1477524844126865E-4	0.0	0.0	32.32689651913755	0.0
37	2.1477524844126865E-4	0.0	0.0	32.67268466912799	0.0
38	2.1477524844126865E-4	0.0	0.0	33.03887646772036	0.0
39	2.1477524844126865E-4	0.0	0.0	33.43771410407579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGA	25	3.887218E-5	45.000004	27
TTTACGT	25	3.887218E-5	45.000004	15
GCAACTC	25	3.887218E-5	45.000004	25
TAGAACG	25	3.887218E-5	45.000004	1
TAGCGAG	25	3.887218E-5	45.000004	1
CCTACAA	25	3.887218E-5	45.000004	27
TCCTCGA	25	3.887218E-5	45.000004	39
GTCTATC	25	3.887218E-5	45.000004	40
CAATTCG	25	3.887218E-5	45.000004	1
ATATGCG	25	3.887218E-5	45.000004	1
TGCGGTC	25	3.887218E-5	45.000004	15
TAAAACG	25	3.887218E-5	45.000004	1
GCGTTAG	25	3.887218E-5	45.000004	1
ATTCACG	25	3.887218E-5	45.000004	28
TATCTAC	25	3.887218E-5	45.000004	18
GATATAG	25	3.887218E-5	45.000004	1
GTACTGG	50	2.1827873E-11	45.000004	2
TATTACG	50	2.1827873E-11	45.000004	1
AATTAGC	25	3.887218E-5	45.000004	15
TATAGCG	25	3.887218E-5	45.000004	1
>>END_MODULE