SRR3553079_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729592
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	8913	1.221641684667595	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGT	8732	1.1968332986107304	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	7646	1.0479829822695423	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	6121	0.8389620500224784	TruSeq Adapter, Index 23 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2207	0.302497834406079	TruSeq Adapter, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1958	0.26836917071459115	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	1791	0.24547966534720778	No Hit
AACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1739	0.23835239421484883	Illumina PCR Primer Index 7 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTAT	1438	0.1970964593910021	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTCTTCTC	1232	0.1688615006743495	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1186	0.1625566069803397	Illumina PCR Primer Index 7 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1103	0.15118038574984374	Illumina PCR Primer Index 7 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1058	0.14501255496222545	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	50	2.1827873E-11	45.000004	1
CGAGTAG	50	2.1827873E-11	45.000004	1
CCGATCA	25	3.889399E-5	45.000004	38
TTCGTAC	25	3.889399E-5	45.000004	41
ATCGTGT	25	3.889399E-5	45.000004	33
CCCAACG	25	3.889399E-5	45.000004	1
CGTAGTT	25	3.889399E-5	45.000004	29
TCGTAGT	25	3.889399E-5	45.000004	28
CGTACAG	25	3.889399E-5	45.000004	1
TCATACT	25	3.889399E-5	45.000004	40
ATACGAT	25	3.889399E-5	45.000004	29
TTCGAGT	25	3.889399E-5	45.000004	14
CGTCAAG	25	3.889399E-5	45.000004	1
GCCAACG	25	3.889399E-5	45.000004	1
CGGACTC	25	3.889399E-5	45.000004	5
GTCATAC	20	7.0315914E-4	45.0	39
AGATCGT	20	7.0315914E-4	45.0	29
GATCGAC	20	7.0315914E-4	45.0	9
CCGATTT	35	1.2111195E-7	45.0	30
CGAAACG	20	7.0315914E-4	45.0	1