##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729592
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744188532768998	31.0	31.0	34.0	30.0	34.0
2	31.94295441835985	33.0	31.0	34.0	30.0	34.0
3	32.08143455520346	34.0	31.0	34.0	30.0	34.0
4	35.74040833781072	37.0	35.0	37.0	35.0	37.0
5	35.51058673888968	37.0	35.0	37.0	33.0	37.0
6	35.531015965087335	37.0	35.0	37.0	33.0	37.0
7	35.56332717464007	37.0	35.0	37.0	33.0	37.0
8	35.638570598361824	37.0	35.0	37.0	35.0	37.0
9	37.333150582791475	39.0	37.0	39.0	34.0	39.0
10	36.948749438042086	39.0	37.0	39.0	33.0	39.0
11	36.95927861051108	39.0	37.0	39.0	33.0	39.0
12	36.80944692376013	39.0	35.0	39.0	33.0	39.0
13	36.816041020186624	39.0	35.0	39.0	33.0	39.0
14	37.92778292525137	40.0	37.0	41.0	33.0	41.0
15	37.991966743056395	40.0	37.0	41.0	33.0	41.0
16	37.83440470838496	40.0	37.0	41.0	33.0	41.0
17	37.818695928683425	40.0	37.0	41.0	33.0	41.0
18	37.690596936369914	40.0	36.0	41.0	33.0	41.0
19	37.709787113893796	40.0	36.0	41.0	32.0	41.0
20	37.7076749745063	39.0	36.0	41.0	33.0	41.0
21	37.71135100165572	39.0	36.0	41.0	33.0	41.0
22	37.66678225638439	39.0	36.0	41.0	33.0	41.0
23	37.523510400333336	39.0	36.0	41.0	32.0	41.0
24	37.41073641158346	39.0	36.0	41.0	32.0	41.0
25	37.41994155637671	39.0	36.0	41.0	32.0	41.0
26	37.383340277853925	39.0	36.0	41.0	32.0	41.0
27	37.324884318907	39.0	36.0	41.0	32.0	41.0
28	37.13339647364555	39.0	35.0	41.0	31.0	41.0
29	36.9519128499216	39.0	35.0	41.0	31.0	41.0
30	37.034904439741666	39.0	35.0	41.0	31.0	41.0
31	36.901653252776896	39.0	35.0	40.0	31.0	41.0
32	36.9125771664163	39.0	35.0	41.0	31.0	41.0
33	36.827931227316085	39.0	35.0	40.0	31.0	41.0
34	36.757659075209155	39.0	35.0	40.0	31.0	41.0
35	36.702156821894974	39.0	35.0	40.0	31.0	41.0
36	36.615047862366914	39.0	35.0	40.0	30.0	41.0
37	36.59908140440137	39.0	35.0	40.0	30.0	41.0
38	36.49300430925778	39.0	35.0	40.0	30.0	41.0
39	36.44685248741762	39.0	35.0	40.0	30.0	41.0
40	36.32976238774548	39.0	35.0	40.0	30.0	41.0
41	36.303698505466066	38.0	35.0	40.0	30.0	41.0
42	36.25945734054101	38.0	35.0	40.0	30.0	41.0
43	36.165341725238214	38.0	35.0	40.0	30.0	41.0
44	36.06592588734526	38.0	35.0	40.0	30.0	41.0
45	35.806575455871226	38.0	35.0	40.0	29.0	41.0
46	35.70474182830952	38.0	35.0	40.0	29.0	41.0
47	35.58510647046568	38.0	34.0	40.0	29.0	41.0
48	35.546723374159804	38.0	34.0	40.0	29.0	41.0
49	35.377974265068694	37.0	34.0	40.0	28.0	41.0
50	35.3087643504863	37.0	34.0	40.0	28.0	41.0
51	33.94368085176372	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	4.0
12	8.0
13	11.0
14	22.0
15	30.0
16	57.0
17	127.0
18	281.0
19	509.0
20	971.0
21	1508.0
22	2105.0
23	2748.0
24	3284.0
25	4045.0
26	4878.0
27	6115.0
28	7628.0
29	10305.0
30	13312.0
31	17871.0
32	24068.0
33	32654.0
34	50019.0
35	61664.0
36	71945.0
37	101799.0
38	148993.0
39	162581.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.948546584940622	35.42281165363656	19.366988673121416	25.261653088301404
2	22.40389149003827	34.5301209443086	19.567648768078598	23.498338797574537
3	20.93361769317646	28.675341834888542	24.593197293829977	25.797843178105023
4	24.181323260123467	29.323649382120422	17.81831489380366	28.67671246395245
5	17.460169519402623	35.28602287305782	18.7855678242086	28.468239783330958
6	20.561080713604316	34.78834745995022	22.10619085735589	22.544380969089573
7	78.94192918782004	8.661827432318336	7.089990021820415	5.306253358041206
8	80.16384499830042	4.698379368194827	5.353951249465454	9.783824384039299
9	75.40460969966776	8.166070899900218	9.950218752398602	6.4791006480334215
10	35.33399489029485	31.242667134508057	14.7039715347756	18.719366440421496
11	26.58526957532429	27.84789306900295	24.502598712705183	21.064238642967574
12	26.673401024134037	21.192940712069213	32.40742771302317	19.726230550773582
13	27.50825118696477	23.56097654579546	26.65037445586026	22.28039781137951
14	20.947872235441178	26.42312415706313	30.30666454676038	22.322339060735317
15	18.740748253818573	27.995783944999395	28.463442581607257	24.800025219574774
16	21.21267777058959	26.886396780666455	27.899702847618947	24.001222601125015
17	20.467329685632517	26.04332284345223	28.15477691641356	25.334570554501695
18	22.795617276505226	25.359241877652167	26.623510126207524	25.221630719635087
19	23.009024221756817	28.76347328369829	27.39257009397033	20.83493240057457
20	23.8544282283797	28.21481046941304	25.141037730676874	22.78972357153039
21	24.766033618789677	25.74425158170594	25.413244662770424	24.076470136733956
22	21.19924560576322	27.544984045877698	25.767278149979717	25.48849219837937
23	23.11661860327416	26.237678044715402	23.320019956359168	27.325683395651268
24	20.64729328172458	29.152594874943805	24.782069978837487	25.41804186449413
25	21.960081799142532	25.872953650807574	25.087857322996964	27.07910722705293
26	23.68762267130122	27.04580094079979	23.263687101832257	26.002889286066733
27	21.136333731729515	25.703406835601267	26.14420114255639	27.01605829011283
28	23.126213006721567	28.08446364543471	25.827448765885592	22.961874581958135
29	22.558772574260683	31.728966326385155	23.084545883178546	22.627715216175616
30	25.97561376769482	25.011924472856062	24.415289641333786	24.597172118115328
31	23.54658494062435	28.821176767289113	24.181460323029857	23.450777969056677
32	23.87005339970833	25.107183192798168	22.391692891369424	28.63107051612408
33	23.770408666761696	25.576897773001896	27.113510016557203	23.539183543679208
34	20.193615061568657	26.835820568207986	26.48439127622013	26.486173094003224
35	22.45364532505839	26.206838890777313	25.56538448886501	25.77413129529929
36	23.058640993870547	27.33856730885207	28.900399127183412	20.70239257009397
37	23.357438129804056	25.287969166328576	23.613882827662582	27.740709876204782
38	23.045345891950568	24.606355332843563	28.86325507955131	23.485043695654557
39	22.298764240835975	21.935958727617628	28.185341944538866	27.579935087007534
40	22.70611519863156	24.00163378984419	27.322530948804264	25.969720062719986
41	21.076437241636423	22.330425772212415	31.38219717321462	25.210939812936545
42	24.31084770666345	24.509040669305584	24.630889593087645	26.54922203094332
43	22.129217425629665	22.915821445410586	27.817739229596818	27.137221899362935
44	24.161449138696696	21.444451145297645	26.203275255211132	28.190824460794527
45	26.10746828364346	23.189124880755273	26.884203774164188	23.81920306143708
46	20.99036173642255	21.847964341714274	32.3228598997796	24.838814022083575
47	22.237360058772573	24.485877038125416	27.116662463404207	26.160100439697803
48	21.27504139299773	21.69226088005351	31.898924330310642	25.13377339663812
49	22.53958376736587	21.85577692737859	28.317196460487505	27.287442844768034
50	21.369340672595094	20.904834482834243	28.08172238730688	29.644102457263788
51	21.152781280496498	20.924160352635447	31.526387350738496	26.396671016129563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2424.0
1	1959.0
2	1494.0
3	1023.0
4	552.0
5	535.5
6	519.0
7	548.5
8	578.0
9	632.5
10	687.0
11	803.0
12	919.0
13	948.0
14	977.0
15	1131.0
16	1285.0
17	1289.5
18	1294.0
19	1459.0
20	1624.0
21	1584.0
22	1544.0
23	2033.0
24	2522.0
25	2875.0
26	3903.5
27	4579.0
28	5629.0
29	6679.0
30	7270.0
31	7861.0
32	9741.5
33	11622.0
34	11962.5
35	12303.0
36	14963.5
37	17624.0
38	18817.5
39	20011.0
40	22649.5
41	25288.0
42	41758.5
43	58229.0
44	61053.5
45	63878.0
46	66492.5
47	69107.0
48	66162.0
49	63217.0
50	59249.5
51	55282.0
52	50131.5
53	44981.0
54	43112.0
55	41243.0
56	39003.0
57	36763.0
58	35513.5
59	34264.0
60	33663.0
61	33062.0
62	30140.0
63	27218.0
64	24588.5
65	21959.0
66	19226.0
67	16493.0
68	14359.5
69	12226.0
70	9944.5
71	7663.0
72	7471.5
73	7280.0
74	5816.5
75	3741.0
76	3129.0
77	2472.0
78	1815.0
79	1387.0
80	959.0
81	646.0
82	333.0
83	265.0
84	197.0
85	167.0
86	137.0
87	99.5
88	62.0
89	42.5
90	23.0
91	21.0
92	19.0
93	39.0
94	59.0
95	42.5
96	26.0
97	13.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	729592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.753232678863053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81226280375289	18.51845348413547
2	10.80223972428382	5.347807066961098
3	3.9942389312193933	2.966109769183408
4	1.951261482669447	1.9320011799128054
5	1.1336523807357148	1.40307805786491
6	0.7634227105570299	1.1338307992047891
7	0.5137909699251944	0.8902591198799948
8	0.4269033798468622	0.845379095419395
9	0.30162942184007485	0.671967293543848
>10	3.474072549217761	21.14036568745585
>50	1.44558054181139	25.450994384629073
>100	0.3686565523939399	12.728608138253161
>500	0.005027134805371907	0.7321092775586627
>1k	0.005027134805371907	1.8958795871358607
>5k	0.002234282135720848	4.343157058861702
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	8913	1.221641684667595	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGT	8732	1.1968332986107304	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	7646	1.0479829822695423	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	6121	0.8389620500224784	TruSeq Adapter, Index 23 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2207	0.302497834406079	TruSeq Adapter, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1958	0.26836917071459115	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	1791	0.24547966534720778	No Hit
AACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1739	0.23835239421484883	Illumina PCR Primer Index 7 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTAT	1438	0.1970964593910021	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTCTTCTC	1232	0.1688615006743495	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1186	0.1625566069803397	Illumina PCR Primer Index 7 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1103	0.15118038574984374	Illumina PCR Primer Index 7 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1058	0.14501255496222545	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3706290639151745E-4	0.0	0.0	0.9425816072544655	0.0
2	1.3706290639151745E-4	0.0	0.0	1.299219289685194	0.0
3	1.3706290639151745E-4	0.0	0.0	1.880914264410794	0.0
4	1.3706290639151745E-4	0.0	0.0	4.475652145308611	0.0
5	1.3706290639151745E-4	0.0	0.0	4.818583537100188	0.0
6	1.3706290639151745E-4	0.0	0.0	6.334773407603153	0.0
7	1.3706290639151745E-4	0.0	0.0	7.465953574052347	0.0
8	1.3706290639151745E-4	0.0	0.0	8.244333819449775	0.0
9	1.3706290639151745E-4	0.0	0.0	10.47503262097172	0.0
10	1.3706290639151745E-4	0.0	0.0	12.139935744909483	0.0
11	1.3706290639151745E-4	0.0	0.0	14.711372931720742	0.0
12	1.3706290639151745E-4	0.0	0.0	15.562259454599284	0.0
13	1.3706290639151745E-4	0.0	0.0	15.98742859022577	0.0
14	1.3706290639151745E-4	0.0	0.0	16.551579512933255	0.0
15	1.3706290639151745E-4	0.0	0.0	16.911643768023772	0.0
16	1.3706290639151745E-4	0.0	0.0	17.51581705939758	0.0
17	1.3706290639151745E-4	0.0	0.0	18.296664437110056	0.0
18	1.3706290639151745E-4	0.0	0.0	19.590675336352373	0.0
19	1.3706290639151745E-4	0.0	0.0	20.025301812519874	0.0
20	1.3706290639151745E-4	0.0	0.0	20.614398184190616	0.0
21	1.3706290639151745E-4	0.0	0.0	21.120982686213665	0.0
22	1.3706290639151745E-4	0.0	0.0	21.653609140451103	0.0
23	1.3706290639151745E-4	0.0	0.0	22.18267195912236	0.0
24	1.3706290639151745E-4	0.0	0.0	22.555894253226462	0.0
25	1.3706290639151745E-4	0.0	0.0	22.89443963201351	0.0
26	1.3706290639151745E-4	0.0	0.0	23.207765436024516	0.0
27	1.3706290639151745E-4	0.0	0.0	23.63896533953223	0.0
28	1.3706290639151745E-4	0.0	0.0	24.009309312602113	0.0
29	1.3706290639151745E-4	0.0	0.0	24.342920426759065	0.0
30	1.3706290639151745E-4	0.0	0.0	24.883222403754427	0.0
31	1.3706290639151745E-4	0.0	0.0	25.20422373052336	0.0
32	1.3706290639151745E-4	0.0	0.0	25.548114562659677	0.0
33	1.3706290639151745E-4	0.0	0.0	25.90228511277536	0.0
34	1.3706290639151745E-4	0.0	0.0	26.22465706860821	0.0
35	1.3706290639151745E-4	0.0	0.0	26.571152095965964	0.0
36	1.3706290639151745E-4	0.0	0.0	26.883244333819448	0.0
37	1.3706290639151745E-4	0.0	0.0	27.194514194234586	0.0
38	1.3706290639151745E-4	0.0	0.0	27.534567264991942	0.0
39	1.3706290639151745E-4	0.0	0.0	27.87489446156208	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	50	2.1827873E-11	45.000004	1
CGAGTAG	50	2.1827873E-11	45.000004	1
CCGATCA	25	3.889399E-5	45.000004	38
TTCGTAC	25	3.889399E-5	45.000004	41
ATCGTGT	25	3.889399E-5	45.000004	33
CCCAACG	25	3.889399E-5	45.000004	1
CGTAGTT	25	3.889399E-5	45.000004	29
TCGTAGT	25	3.889399E-5	45.000004	28
CGTACAG	25	3.889399E-5	45.000004	1
TCATACT	25	3.889399E-5	45.000004	40
ATACGAT	25	3.889399E-5	45.000004	29
TTCGAGT	25	3.889399E-5	45.000004	14
CGTCAAG	25	3.889399E-5	45.000004	1
GCCAACG	25	3.889399E-5	45.000004	1
CGGACTC	25	3.889399E-5	45.000004	5
GTCATAC	20	7.0315914E-4	45.0	39
AGATCGT	20	7.0315914E-4	45.0	29
GATCGAC	20	7.0315914E-4	45.0	9
CCGATTT	35	1.2111195E-7	45.0	30
CGAAACG	20	7.0315914E-4	45.0	1
>>END_MODULE