##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454504
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65614164011758	31.0	31.0	34.0	30.0	34.0
2	31.860225652579516	33.0	31.0	34.0	30.0	34.0
3	31.981804340555858	33.0	31.0	34.0	30.0	34.0
4	35.63887006494992	37.0	35.0	37.0	33.0	37.0
5	35.38616821854153	37.0	35.0	37.0	33.0	37.0
6	35.396641173675036	37.0	35.0	37.0	33.0	37.0
7	35.3275504726031	37.0	35.0	37.0	33.0	37.0
8	35.49409906183444	37.0	35.0	37.0	33.0	37.0
9	37.26075238061711	39.0	37.0	39.0	34.0	39.0
10	36.78950680302044	39.0	37.0	39.0	32.0	39.0
11	36.80641534507947	39.0	37.0	39.0	32.0	39.0
12	36.586654902927144	39.0	35.0	39.0	32.0	39.0
13	36.650269304560574	39.0	35.0	39.0	32.0	39.0
14	37.697938852023306	39.0	36.0	41.0	33.0	41.0
15	37.79993795434144	39.0	37.0	41.0	33.0	41.0
16	37.58225010120923	39.0	36.0	41.0	32.0	41.0
17	37.55522503652333	39.0	36.0	41.0	32.0	41.0
18	37.4441875099009	39.0	36.0	41.0	32.0	41.0
19	37.45628201291958	39.0	36.0	41.0	32.0	41.0
20	37.52332212697798	39.0	36.0	41.0	32.0	41.0
21	37.577843099290654	39.0	36.0	41.0	32.0	41.0
22	37.535366025381514	39.0	36.0	41.0	32.0	41.0
23	37.343618538010666	39.0	36.0	41.0	32.0	41.0
24	37.217078397549855	39.0	36.0	41.0	32.0	41.0
25	37.23794070019186	39.0	36.0	41.0	32.0	41.0
26	37.22658326439371	39.0	36.0	41.0	32.0	41.0
27	37.15857946244697	39.0	36.0	41.0	31.0	41.0
28	37.01997782197736	39.0	35.0	41.0	31.0	41.0
29	36.9450895921708	39.0	35.0	40.0	31.0	41.0
30	36.99117939556088	39.0	35.0	40.0	31.0	41.0
31	36.911890764437715	39.0	35.0	40.0	31.0	41.0
32	36.82600373154032	39.0	35.0	40.0	31.0	41.0
33	36.65601622868006	39.0	35.0	40.0	30.0	41.0
34	36.64204275429919	39.0	35.0	40.0	30.0	41.0
35	36.606799940154545	39.0	35.0	40.0	30.0	41.0
36	36.619297079893684	39.0	35.0	40.0	30.0	41.0
37	36.519999823983945	39.0	35.0	40.0	30.0	41.0
38	36.38495590797881	39.0	35.0	40.0	30.0	41.0
39	36.14879957052083	38.0	35.0	40.0	30.0	41.0
40	35.84327750690863	38.0	35.0	40.0	29.0	41.0
41	35.938253568725465	38.0	35.0	40.0	30.0	41.0
42	35.96654374878989	38.0	35.0	40.0	30.0	41.0
43	35.97057231619524	38.0	35.0	40.0	30.0	41.0
44	35.940185344903455	38.0	35.0	40.0	30.0	41.0
45	35.775546529843524	38.0	35.0	40.0	29.0	41.0
46	35.45372757995529	38.0	34.0	40.0	28.0	41.0
47	35.35134564272261	38.0	34.0	40.0	27.0	41.0
48	35.33668130533505	38.0	34.0	40.0	28.0	41.0
49	35.18206660447433	37.0	34.0	40.0	27.0	41.0
50	35.1589050921444	37.0	34.0	40.0	27.0	41.0
51	33.56706431626564	36.0	31.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	7.0
12	6.0
13	10.0
14	17.0
15	23.0
16	54.0
17	92.0
18	149.0
19	304.0
20	563.0
21	979.0
22	1377.0
23	1837.0
24	2262.0
25	2651.0
26	3233.0
27	4081.0
28	5297.0
29	6903.0
30	9193.0
31	12549.0
32	16561.0
33	22380.0
34	32581.0
35	39662.0
36	45401.0
37	62590.0
38	89250.0
39	94467.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.666731205885977	38.65026490415926	18.51930896097724	25.16369492897752
2	21.323244679914808	37.7998873497263	18.582894760002112	22.293973210356786
3	19.970781335257776	27.612958301797125	28.156627884463063	24.259632478482036
4	26.27127594036576	28.319662753243097	18.58069455934381	26.828366747047333
5	16.62955661556334	38.33299596923239	17.84076707795751	27.19668033724676
6	20.559114991287206	33.786281308855365	25.49438508792002	20.16021861193741
7	74.3084769330963	12.071840951894814	7.761427842219211	5.8582542727896785
8	76.22617182687061	4.463987115624945	5.709300688222766	13.600540369281678
9	73.8347737313643	7.0861862601869285	11.298470420502351	7.78056958794642
10	35.512118705225916	29.971793427560595	15.602502948268882	18.913584918944608
11	26.09195958671431	26.29151778642212	25.584373294844493	22.03214933201908
12	25.66204037808248	22.987256437787128	33.29695668244944	18.053746501680955
13	25.65896009716086	26.75818034604756	24.3480805449457	23.23477901184588
14	19.12788446306303	25.496365268512488	33.705973984827416	21.669776283597063
15	18.841418337352366	28.914156971115766	25.601314839913403	26.64310985161847
16	19.534701564782708	27.125613855983666	29.498310245894427	23.8413743333392
17	18.40489852674564	26.511537852252125	28.920757573090665	26.16280604791157
18	20.76373365250911	26.24157323147871	26.262915177864222	26.73177793814796
19	19.879252987872494	29.918988611761392	29.364758065935614	20.8370003344305
20	22.945012585147765	31.03669054617781	24.752477425941247	21.26581944273318
21	21.41939344868252	26.064237058419724	26.90229348916621	25.61407600373154
22	21.46801788323095	28.983683311918046	25.354012285920476	24.19428651893053
23	23.342368824036754	27.36983612905497	22.204865083695633	27.082929963212642
24	18.52810976361044	32.78430992906553	24.97887807368032	23.70870223364371
25	20.505430095224682	27.115492932955487	24.762378328903594	27.616698642916234
26	24.140161582736347	27.283148223117948	22.72631263971274	25.850377554432963
27	19.263416823614314	25.711104852762574	26.85146885395948	28.174009469663634
28	22.05789167972119	28.529341875979092	23.90386003203492	25.5089064122648
29	21.28848150951367	29.484889021878796	23.076584584514105	26.150044884093433
30	22.343917765300194	24.819363525953566	24.18900603735061	28.64771267139563
31	24.802421980884656	28.289299984158557	20.060109481984757	26.84816855297203
32	24.17558481333498	24.326738598560187	21.4251139703941	30.072562617710734
33	21.613891186876245	27.554652984352174	25.26886452044426	25.56259130832732
34	19.207311706827664	28.50865998979107	23.10936757432278	29.174660729058488
35	20.20884304648584	30.102045306531956	23.172293313150156	26.516818333832042
36	20.420722369880135	26.479854962772603	25.353572245788815	27.745850421558444
37	23.252600637178112	28.163228486437962	19.65571260098921	28.928458275394714
38	21.85415309876261	26.395147237428052	26.547621143048243	25.20307852076109
39	23.008818404238465	25.72738633763399	25.869959340291835	25.393835917835705
40	21.127646841391932	29.928449474592085	25.423978666854417	23.519925017161565
41	19.63613081513034	26.573363490750356	29.23846654814919	24.552039145970113
42	23.83103339024519	27.93088729692148	22.14149930473659	26.096580008096737
43	21.686497808600144	23.401114181613362	27.23276362804288	27.67962438174362
44	22.208825444880574	21.810589125728267	25.417598084945347	30.562987344445812
45	26.927815816802493	24.108038653125167	25.756869026455213	23.20727650361713
46	19.61918927006143	22.74567440550578	33.85580764965765	23.779328674775137
47	22.0286290109658	26.30889937162269	24.667109640399204	26.995361977012305
48	20.490688750814073	23.689340467850666	31.652526710435993	24.167444070899265
49	23.10562723320367	23.034560751940578	28.147607061764035	25.71220495309172
50	21.97054371358668	21.017636808476933	26.408568461443682	30.603251016492706
51	20.433263513632443	21.097284072307396	31.887068100610776	26.582384313449385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1787.0
1	1417.0
2	1047.0
3	703.5
4	360.0
5	340.0
6	320.0
7	332.5
8	345.0
9	374.5
10	404.0
11	434.5
12	465.0
13	548.0
14	631.0
15	642.0
16	653.0
17	668.0
18	683.0
19	802.5
20	922.0
21	914.0
22	906.0
23	1263.5
24	1621.0
25	1667.5
26	1928.5
27	2143.0
28	3061.0
29	3979.0
30	4942.5
31	5906.0
32	6742.0
33	7578.0
34	8151.0
35	8724.0
36	9753.5
37	10783.0
38	11613.0
39	12443.0
40	13632.0
41	14821.0
42	17710.5
43	20600.0
44	36272.0
45	51944.0
46	51373.0
47	50802.0
48	48367.5
49	45933.0
50	42265.5
51	38598.0
52	35696.0
53	32794.0
54	29107.0
55	25420.0
56	24537.5
57	23655.0
58	21464.0
59	19273.0
60	17887.0
61	16501.0
62	15228.0
63	13955.0
64	12333.0
65	10711.0
66	8995.0
67	7279.0
68	6753.0
69	6227.0
70	5200.5
71	4174.0
72	3677.0
73	3180.0
74	2790.0
75	1896.0
76	1392.0
77	1141.5
78	891.0
79	627.0
80	363.0
81	221.0
82	79.0
83	55.0
84	31.0
85	20.5
86	10.0
87	24.0
88	38.0
89	22.0
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	454504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.3359524564181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59606191317967	20.17230244894357
2	9.68317956586433	5.100315133471245
3	3.4961879803265346	2.762263212857401
4	1.7088876330778418	1.8002073383239559
5	0.9812678822505628	1.2921312146980448
6	0.6503567538407047	1.0276658729315526
7	0.48752953572049	0.8987688271684089
8	0.3876695010304397	0.816771643835279
9	0.30207940026098135	0.7159993851002849
>10	4.357492684408773	28.47421723670657
>50	1.217547048947474	21.534994060275885
>100	0.11999257615402398	4.857080467226997
>500	0.0033564356966160557	0.6028534289364961
>1k	0.005873762469078097	3.364311071161736
>5k	8.391089241540139E-4	1.981151389448566
>10k+	0.0016782178483080278	4.59896726891401
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	10545	2.320111594177389	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGT	10266	2.258725995810818	No Hit
AATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	8965	1.9724798901659832	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	4653	1.0237533663070073	No Hit
AATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	2356	0.5183672750954887	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	1897	0.41737806487951695	No Hit
AACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG	1692	0.37227395138436625	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTAT	1649	0.3628130885536761	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCGTAATC	1526	0.33575062045658566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1451	0.3192491155193354	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	778	0.17117561121574287	No Hit
AAACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	705	0.15511414641015261	No Hit
AATGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	679	0.1493936246985725	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	566	0.1245313572597821	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATG	483	0.10626969179589178	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	455	0.10010912995265168	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.200200658300037E-4	0.0	0.0	1.1579656064633095	0.0
2	2.200200658300037E-4	0.0	0.0	1.4730343407318747	0.0
3	2.200200658300037E-4	0.0	0.0	2.2171422033689474	0.0
4	2.200200658300037E-4	0.0	0.0	5.785647651065777	0.0
5	2.200200658300037E-4	0.0	0.0	6.152861140936054	0.0
6	2.200200658300037E-4	0.0	0.0	7.834694524140602	0.0
7	2.200200658300037E-4	0.0	0.0	9.132152852340134	0.0
8	2.200200658300037E-4	0.0	0.0	9.926865330118106	0.0
9	2.200200658300037E-4	0.0	0.0	13.279531093235702	0.0
10	2.200200658300037E-4	0.0	0.0	14.82935243694225	0.0
11	2.200200658300037E-4	0.0	0.0	18.552531990917572	0.0
12	2.200200658300037E-4	0.0	0.0	19.313141358491894	0.0
13	2.200200658300037E-4	0.0	0.0	19.752081389822752	0.0
14	2.200200658300037E-4	0.0	0.0	20.449104958372203	0.0
15	2.200200658300037E-4	0.0	0.0	20.81587840811082	0.0
16	2.200200658300037E-4	0.0	0.0	21.42357382993329	0.0
17	2.200200658300037E-4	0.0	0.0	22.07417316459261	0.0
18	2.200200658300037E-4	0.0	0.0	23.420035907274745	0.0
19	2.200200658300037E-4	0.0	0.0	23.8127717247813	0.0
20	2.200200658300037E-4	0.0	0.0	24.222449087356768	0.0
21	2.200200658300037E-4	0.0	0.0	24.671730061781634	0.0
22	2.200200658300037E-4	0.0	0.0	25.12145107633816	0.0
23	2.200200658300037E-4	0.0	0.0	25.58173305405453	0.0
24	2.200200658300037E-4	0.0	0.0	25.963467868269586	0.0
25	2.200200658300037E-4	0.0	0.0	26.2598348969426	0.0
26	2.200200658300037E-4	0.0	0.0	26.533979898966784	0.0
27	2.200200658300037E-4	0.0	0.0	26.8435481315896	0.0
28	2.200200658300037E-4	0.0	0.0	27.138375019801806	0.0
29	2.200200658300037E-4	0.0	0.0	27.44112263038389	0.0
30	2.200200658300037E-4	0.0	0.0	27.94078819988383	0.0
31	2.200200658300037E-4	0.0	0.0	28.230334606516113	0.0
32	2.200200658300037E-4	0.0	0.0	28.53814267861229	0.0
33	2.200200658300037E-4	0.0	0.0	28.860912115184906	0.0
34	2.200200658300037E-4	0.0	0.0	29.1629996655695	0.0
35	2.200200658300037E-4	0.0	0.0	29.488629362997905	0.0
36	2.200200658300037E-4	0.0	0.0	29.77993593015683	0.0
37	2.200200658300037E-4	0.0	0.0	30.085543801594707	0.0
38	2.200200658300037E-4	0.0	0.0	30.403912836850722	0.0
39	2.200200658300037E-4	0.0	0.0	30.713701089539367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	25	3.8870705E-5	45.000004	10
CGGTTAG	25	3.8870705E-5	45.000004	1
GAATCGT	25	3.8870705E-5	45.000004	29
CGCGATG	30	2.1625947E-6	45.000004	1
AAAGTCG	25	3.8870705E-5	45.000004	33
GTATGCG	45	3.8380676E-10	45.000004	1
TCTCGCG	30	2.1625947E-6	45.000004	1
ACGCTCT	25	3.8870705E-5	45.000004	45
TTAACGG	25	3.8870705E-5	45.000004	2
TTAGTTC	25	3.8870705E-5	45.000004	21
TTACGGA	25	3.8870705E-5	45.000004	40
ACGTATT	25	3.8870705E-5	45.000004	11
ACGTAAG	25	3.8870705E-5	45.000004	1
CGATAGG	30	2.1625947E-6	45.000004	2
GCGTACT	25	3.8870705E-5	45.000004	29
TAGTACG	30	2.1625947E-6	45.000004	1
AAGTCGA	25	3.8870705E-5	45.000004	34
GTCGCAT	20	7.028782E-4	45.0	41
GTACCAT	20	7.028782E-4	45.0	9
ATCTAGA	20	7.028782E-4	45.0	22
>>END_MODULE