SRR3553074_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480678
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3261	0.6784167363598916	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1589	0.3305747298607384	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGT	1026	0.2134485039881168	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	1026	0.2134485039881168	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	940	0.19555710891698808	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	733	0.15249293705973646	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	666	0.13855429206246178	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	655	0.13626585780917788	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	629	0.130856831392325	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACA	25	3.8874066E-5	45.000004	19
ATTACGA	25	3.8874066E-5	45.000004	37
CGTTATT	25	3.8874066E-5	45.000004	7
CGTTAAG	25	3.8874066E-5	45.000004	1
GCTTACG	25	3.8874066E-5	45.000004	1
TTACGAA	25	3.8874066E-5	45.000004	38
TACTACG	25	3.8874066E-5	45.000004	1
GAATTCG	25	3.8874066E-5	45.000004	12
ATTGCGA	25	3.8874066E-5	45.000004	16
TAGTGCG	30	2.1628566E-6	45.000004	1
CGTTTAT	30	2.1628566E-6	45.000004	39
CAATACG	25	3.8874066E-5	45.000004	1
AACGTAC	20	7.029188E-4	45.0	16
ACTATCG	20	7.029188E-4	45.0	1
ACGCCTA	20	7.029188E-4	45.0	40
CCGATCG	20	7.029188E-4	45.0	26
CCGTGCA	20	7.029188E-4	45.0	31
ATGTCGA	20	7.029188E-4	45.0	28
TCTAGCG	40	6.8012014E-9	45.0	1
CTAACGG	40	6.8012014E-9	45.0	2