##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480678
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813694406650605	33.0	31.0	34.0	30.0	34.0
2	32.015815161084966	33.0	31.0	34.0	30.0	34.0
3	32.18086744140568	34.0	31.0	34.0	30.0	34.0
4	35.83117388355615	37.0	35.0	37.0	35.0	37.0
5	35.60505161459439	37.0	35.0	37.0	33.0	37.0
6	35.006721755520324	37.0	35.0	37.0	33.0	37.0
7	35.385047786668	37.0	35.0	37.0	33.0	37.0
8	35.738517261035454	37.0	35.0	37.0	35.0	37.0
9	37.373045989206915	39.0	37.0	39.0	35.0	39.0
10	37.00842351844686	39.0	37.0	39.0	33.0	39.0
11	36.995812165316494	39.0	37.0	39.0	33.0	39.0
12	36.91045772845855	39.0	37.0	39.0	33.0	39.0
13	36.86801351424446	39.0	37.0	39.0	33.0	39.0
14	38.02317975859099	40.0	37.0	41.0	33.0	41.0
15	38.05346614573582	40.0	37.0	41.0	33.0	41.0
16	37.94728071598867	40.0	37.0	41.0	33.0	41.0
17	37.970683076820656	40.0	37.0	41.0	33.0	41.0
18	37.92224732565251	40.0	37.0	41.0	33.0	41.0
19	37.92297962461357	40.0	37.0	41.0	33.0	41.0
20	37.83109898934422	40.0	37.0	41.0	33.0	41.0
21	37.81633442762099	40.0	37.0	41.0	33.0	41.0
22	37.79946658677951	40.0	36.0	41.0	33.0	41.0
23	37.70394318025789	40.0	36.0	41.0	33.0	41.0
24	37.58344047366428	39.0	36.0	41.0	32.0	41.0
25	37.57926512134943	39.0	36.0	41.0	33.0	41.0
26	37.51502877185975	39.0	36.0	41.0	33.0	41.0
27	37.40878093026933	39.0	36.0	41.0	32.0	41.0
28	37.31200304569795	39.0	36.0	41.0	32.0	41.0
29	37.10164600834654	39.0	35.0	41.0	31.0	41.0
30	37.08917196127137	39.0	35.0	41.0	31.0	41.0
31	37.07963751201428	39.0	35.0	41.0	31.0	41.0
32	37.03975010297954	39.0	35.0	41.0	31.0	41.0
33	36.779238908375255	39.0	35.0	41.0	31.0	41.0
34	36.775354811328995	39.0	35.0	41.0	31.0	41.0
35	36.705133166069594	39.0	35.0	41.0	31.0	41.0
36	36.62972717702911	39.0	35.0	40.0	30.0	41.0
37	36.59195760987605	39.0	35.0	40.0	30.0	41.0
38	36.42811195852525	39.0	35.0	40.0	30.0	41.0
39	36.40242948501908	39.0	35.0	40.0	30.0	41.0
40	36.318104843575114	39.0	35.0	40.0	30.0	41.0
41	36.29008608673582	39.0	35.0	40.0	30.0	41.0
42	36.20119081796962	38.0	35.0	40.0	30.0	41.0
43	36.11449660687612	38.0	35.0	40.0	30.0	41.0
44	36.02187535106662	38.0	35.0	40.0	30.0	41.0
45	35.858452019855285	38.0	35.0	40.0	29.0	41.0
46	35.71382921623207	38.0	35.0	40.0	29.0	41.0
47	35.59975492949542	38.0	34.0	40.0	28.0	41.0
48	35.5152638564694	38.0	34.0	40.0	28.0	41.0
49	35.38613999392525	38.0	34.0	40.0	28.0	41.0
50	35.2886485339458	37.0	34.0	40.0	28.0	41.0
51	34.00679873012703	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	4.0
12	5.0
13	10.0
14	12.0
15	18.0
16	46.0
17	100.0
18	224.0
19	450.0
20	676.0
21	1022.0
22	1465.0
23	1930.0
24	2393.0
25	2841.0
26	3377.0
27	4145.0
28	5137.0
29	6708.0
30	8571.0
31	11395.0
32	14755.0
33	20322.0
34	31455.0
35	38885.0
36	45887.0
37	64948.0
38	99437.0
39	114422.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.09411705965324	32.831542113431446	20.340227761620046	26.734113065295272
2	22.866659177245474	31.21257889897187	20.424275710558838	25.496486213223825
3	21.984155713388173	30.29907755295645	20.816014046825526	26.900752686829854
4	21.51128198086869	30.7975401412172	19.590037405498066	28.10114047241605
5	17.70790425191084	34.40827331394406	18.3561552640229	29.5276671701222
6	20.36685681474917	37.952641893325676	20.007780676461167	21.67272061546399
7	82.51885877864183	6.532647635215258	5.952217492791432	4.996276093351475
8	84.54849192182708	5.03330712035916	4.632207007601762	5.785993950211992
9	76.94568921398525	8.651945793233724	9.167259579177745	5.235105413603285
10	36.08527954264601	32.017275598217516	13.642604820690774	18.254840038445696
11	27.57272019938504	29.235787783089716	23.92807659181406	19.263415425711184
12	28.389483188329816	21.41391950536534	29.82516362304911	20.371433683255734
13	27.23007917982516	23.696528653277245	26.748259749770114	22.325132417127474
14	20.955816575753413	27.73311863659248	28.423185583696366	22.88787920395774
15	19.82741044940688	28.765202484823522	28.82636609122947	22.581020974540127
16	21.59200129816634	26.661923366578044	27.832977585826686	23.913097749428932
17	21.600946995701907	26.889726594518574	26.91801996346827	24.59130644631125
18	23.00770994303879	27.4520573023937	25.60591497842631	23.9343177761412
19	24.365167534191286	29.821626951930398	25.45820694935071	20.354998564527605
20	25.099130811062707	27.95509675916102	23.716500443124087	23.229271986652186
21	24.408855824481254	27.959673627667588	25.042752112640894	22.588718435210264
22	22.14143355843205	27.127931796337673	24.97160261131152	25.759032033918754
23	23.388838265949346	27.317247720927522	24.496440444538756	24.797473568584376
24	21.80628196006474	27.741440215695327	25.469649120617127	24.9826287036228
25	23.249867894931743	28.381993767137253	23.493066044212547	24.875072293718457
26	24.165241596245306	27.68901426734737	23.648887612913427	24.4968565234939
27	21.352339820004243	27.874793520818507	25.028605428166053	25.744261231011194
28	23.065960996758744	27.843171520227678	26.264776003894497	22.82609147911908
29	23.380308647368924	28.744606576543962	24.662872026595767	23.212212749491343
30	23.276704987538434	27.076754084855143	26.663379642921043	22.983161284685384
31	25.426792988237445	27.915777297900053	23.18537565688465	23.47205405697785
32	26.670869064113607	25.614028518051583	24.493735931330328	23.22136648650448
33	24.176475728034152	26.482593336911613	26.650273155834054	22.690657779220185
34	25.29531203841241	25.924215379110336	25.925047537020625	22.855425045456627
35	25.621934018199294	24.893587807222296	27.226750548184025	22.257727626394384
36	25.212096247383904	27.024952254939898	27.54442683043534	20.218524667240857
37	24.836793029845346	27.79428224299843	25.926087734408483	21.442836992747743
38	26.03447630222311	25.746757704742052	27.139582007081664	21.079183985953172
39	25.783372652794593	24.216835386682977	26.494035508178033	23.505756452344396
40	26.797357066476934	24.81432476626765	26.321570781271454	22.066747385983966
41	24.10074935819821	23.582731059045763	28.61687033731521	23.699649245440817
42	26.44056936244222	25.098298653152423	24.50850673423789	23.952625250167472
43	23.315816409321833	24.05248419940168	27.633467726835843	24.998231664440645
44	24.07245598924852	22.404187418604554	27.313711049808813	26.209645542338112
45	25.49045306837425	22.946754376110412	27.9255551533459	23.637237402169436
46	23.036211351466054	24.443598417235656	29.170463387132344	23.34972684416595
47	23.600622454116895	25.346697789372513	27.177653231477205	23.87502652503339
48	24.207265570714696	22.97005479759839	29.61920454025356	23.20347509143335
49	24.270509571896362	23.81427899758258	27.881866863055933	24.03334456746512
50	22.968806560732965	22.82484324225365	27.75600297912532	26.450347217888066
51	23.48162387294613	22.1482988611919	29.33190202172764	25.038175244134326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1904.0
1	1542.0
2	1180.0
3	819.0
4	458.0
5	440.5
6	423.0
7	466.0
8	509.0
9	539.5
10	570.0
11	639.5
12	709.0
13	657.5
14	606.0
15	727.0
16	848.0
17	879.0
18	910.0
19	1027.0
20	1144.0
21	1226.0
22	1308.0
23	1793.0
24	2278.0
25	2519.5
26	3440.5
27	4120.0
28	4530.5
29	4941.0
30	5996.0
31	7051.0
32	8091.5
33	9132.0
34	9700.0
35	10268.0
36	11531.5
37	12795.0
38	13713.0
39	14631.0
40	15800.0
41	16969.0
42	18848.5
43	20728.0
44	25506.0
45	30284.0
46	34917.5
47	39551.0
48	43471.0
49	47391.0
50	45436.0
51	43481.0
52	39390.5
53	35300.0
54	32223.5
55	29147.0
56	27056.0
57	24965.0
58	25265.5
59	25566.0
60	24103.5
61	22641.0
62	20872.0
63	19103.0
64	17072.5
65	15042.0
66	12268.5
67	9495.0
68	8521.5
69	7548.0
70	6189.0
71	4830.0
72	4444.5
73	4059.0
74	3120.5
75	1872.0
76	1562.0
77	1225.0
78	888.0
79	849.0
80	810.0
81	572.0
82	334.0
83	218.0
84	102.0
85	73.5
86	45.0
87	45.5
88	46.0
89	36.5
90	27.0
91	23.0
92	19.0
93	14.5
94	10.0
95	7.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.073250926913584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55356099952172	22.25660888287881
2	9.57230669161087	5.565961487891537
3	3.3595093750352323	2.9301557715515387
4	1.6008778353691813	1.861708920440899
5	0.9295040168793176	1.3511851760153264
6	0.6617224739071362	1.1543054116728122
7	0.4799663647128058	0.9767927790441747
8	0.408556220956933	0.950244602370597
9	0.3256432184304372	0.852075630186825
>10	4.9599491656078225	35.97921291435728
>50	1.0582757575204156	19.80363042725543
>100	0.08358634073821963	4.094023815545335
>500	0.00363418872774868	0.7655955550593967
>1k	0.002907350982198944	1.4584986257300459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3261	0.6784167363598916	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1589	0.3305747298607384	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGT	1026	0.2134485039881168	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	1026	0.2134485039881168	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	940	0.19555710891698808	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	733	0.15249293705973646	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	666	0.13855429206246178	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	655	0.13626585780917788	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	629	0.130856831392325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7491501587341214	0.0
2	0.0	0.0	0.0	1.0493511248694551	0.0
3	0.0	0.0	0.0	1.4053066709938877	0.0
4	0.0	0.0	0.0	2.9643545159129396	0.0
5	0.0	0.0	0.0	3.1986069676581828	0.0
6	0.0	0.0	0.0	4.441434806668913	0.0
7	0.0	0.0	0.0	5.308127270230799	0.0
8	0.0	0.0	0.0	6.032312691656369	0.0
9	0.0	0.0	0.0	7.13200937009807	0.0
10	0.0	0.0	0.0	8.645496569429016	0.0
11	0.0	0.0	0.0	10.124033136527988	0.0
12	0.0	0.0	0.0	10.81077145199073	0.0
13	0.0	0.0	0.0	11.125535181556051	0.0
14	0.0	0.0	0.0	11.48398720141134	0.0
15	0.0	0.0	0.0	11.754438522253983	0.0
16	0.0	0.0	0.0	12.33986161213952	0.0
17	0.0	0.0	0.0	13.038250138346253	0.0
18	0.0	0.0	0.0	14.163327633051647	0.0
19	0.0	0.0	0.0	14.542791640141633	0.0
20	0.0	0.0	0.0	15.080781729140922	0.0
21	0.0	0.0	0.0	15.576123725238102	0.0
22	0.0	0.0	0.0	16.096430458643834	0.0
23	0.0	0.0	0.0	16.63754113980669	0.0
24	0.0	0.0	0.0	17.011388081002252	0.0
25	0.0	0.0	0.0	17.333225152804996	0.0
26	0.0	0.0	0.0	17.647156724460032	0.0
27	0.0	0.0	0.0	18.07301353504841	0.0
28	0.0	0.0	0.0	18.461631279151533	0.0
29	0.0	0.0	0.0	18.8267405622891	0.0
30	0.0	0.0	0.0	19.38969538859694	0.0
31	0.0	0.0	0.0	19.716109328906253	0.0
32	0.0	0.0	0.0	20.055005637869844	0.0
33	0.0	0.0	0.0	20.404303920711996	0.0
34	0.0	0.0	0.0	20.746944940271867	0.0
35	0.0	0.0	0.0	21.113718539230003	0.0
36	0.0	0.0	0.0	21.446789742821597	0.0
37	0.0	0.0	0.0	21.792135275589896	0.0
38	0.0	0.0	0.0	22.12250196597306	0.0
39	0.0	0.0	0.0	22.471384169860073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACA	25	3.8874066E-5	45.000004	19
ATTACGA	25	3.8874066E-5	45.000004	37
CGTTATT	25	3.8874066E-5	45.000004	7
CGTTAAG	25	3.8874066E-5	45.000004	1
GCTTACG	25	3.8874066E-5	45.000004	1
TTACGAA	25	3.8874066E-5	45.000004	38
TACTACG	25	3.8874066E-5	45.000004	1
GAATTCG	25	3.8874066E-5	45.000004	12
ATTGCGA	25	3.8874066E-5	45.000004	16
TAGTGCG	30	2.1628566E-6	45.000004	1
CGTTTAT	30	2.1628566E-6	45.000004	39
CAATACG	25	3.8874066E-5	45.000004	1
AACGTAC	20	7.029188E-4	45.0	16
ACTATCG	20	7.029188E-4	45.0	1
ACGCCTA	20	7.029188E-4	45.0	40
CCGATCG	20	7.029188E-4	45.0	26
CCGTGCA	20	7.029188E-4	45.0	31
ATGTCGA	20	7.029188E-4	45.0	28
TCTAGCG	40	6.8012014E-9	45.0	1
CTAACGG	40	6.8012014E-9	45.0	2
>>END_MODULE