FastQCFastQC Report
Sat 18 Jun 2016
SRR3553069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506219
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT28050.5541080046383087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18880.37296110971733576No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7660.1513179078620123No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7570.14954002121611398No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC6630.13097098291450934No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT6360.12563732297681438No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC6300.12445206521288217No Hit
CCACCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTC5140.10153708177685943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA207.02954E-445.00000441
TCACGAT351.2101191E-745.00000411
ATTTACG207.02954E-445.00000445
TCGCATA207.02954E-445.00000429
CGAACCT207.02954E-445.00000445
AGTTTCG207.02954E-445.00000424
GACACGT207.02954E-445.00000433
CGCATAC207.02954E-445.00000430
CGGCACG406.8012014E-945.0000041
ATAGATC207.02954E-445.00000420
TTCCGTA207.02954E-445.00000426
ATGTCGT207.02954E-445.00000410
ATGTCGA207.02954E-445.00000412
GACCCGA207.02954E-445.00000414
GTTACCG207.02954E-445.00000439
CCGTAAG207.02954E-445.0000041
TCTACGT207.02954E-445.00000427
GTTCGAC207.02954E-445.00000411
CTACGTA207.02954E-445.00000434
TGCGATC207.02954E-445.00000420