##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506219
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803118808262827	33.0	31.0	34.0	30.0	34.0
2	32.00523093759816	33.0	31.0	34.0	30.0	34.0
3	32.155197651609285	34.0	31.0	34.0	30.0	34.0
4	35.82190711925076	37.0	35.0	37.0	35.0	37.0
5	35.601346452819826	37.0	35.0	37.0	33.0	37.0
6	35.62389598177864	37.0	35.0	37.0	33.0	37.0
7	35.69896625768689	37.0	35.0	37.0	35.0	37.0
8	35.753010060862984	37.0	35.0	37.0	35.0	37.0
9	37.42358346881488	39.0	37.0	39.0	35.0	39.0
10	36.99787443774335	39.0	37.0	39.0	33.0	39.0
11	36.983143659167276	39.0	37.0	39.0	33.0	39.0
12	36.90648118699614	39.0	37.0	39.0	33.0	39.0
13	36.871834127126796	39.0	37.0	39.0	33.0	39.0
14	38.04582601601283	40.0	37.0	41.0	33.0	41.0
15	38.06140425389012	40.0	37.0	41.0	33.0	41.0
16	37.953510239639364	40.0	37.0	41.0	33.0	41.0
17	37.93020807200046	40.0	37.0	41.0	33.0	41.0
18	37.85587265590584	40.0	37.0	41.0	33.0	41.0
19	37.90435364930988	40.0	37.0	41.0	33.0	41.0
20	37.78723042793732	40.0	36.0	41.0	33.0	41.0
21	37.741793571556975	40.0	36.0	41.0	33.0	41.0
22	37.71026571503638	39.0	36.0	41.0	33.0	41.0
23	37.59242146185742	39.0	36.0	41.0	32.0	41.0
24	37.479737030810774	39.0	36.0	41.0	32.0	41.0
25	37.488689677787676	39.0	36.0	41.0	32.0	41.0
26	37.47520341986373	39.0	36.0	41.0	32.0	41.0
27	37.37479825925143	39.0	36.0	41.0	32.0	41.0
28	37.257311558831255	39.0	36.0	41.0	32.0	41.0
29	37.06813849341886	39.0	35.0	41.0	31.0	41.0
30	37.11935743225758	39.0	35.0	41.0	31.0	41.0
31	37.027948378073525	39.0	35.0	41.0	31.0	41.0
32	36.99812926816259	39.0	35.0	41.0	31.0	41.0
33	36.92737530594466	39.0	35.0	41.0	31.0	41.0
34	36.82478136932829	39.0	35.0	41.0	31.0	41.0
35	36.78404801084116	39.0	35.0	41.0	31.0	41.0
36	36.71259474654251	39.0	35.0	41.0	31.0	41.0
37	36.64286603229037	39.0	35.0	41.0	31.0	41.0
38	36.547128811838355	39.0	35.0	40.0	30.0	41.0
39	36.43801990837957	39.0	35.0	40.0	30.0	41.0
40	36.34398945910762	39.0	35.0	40.0	30.0	41.0
41	36.29034271728244	39.0	35.0	40.0	30.0	41.0
42	36.24992740296196	38.0	35.0	40.0	30.0	41.0
43	36.16561014106543	38.0	35.0	40.0	30.0	41.0
44	36.09120360950498	38.0	35.0	40.0	30.0	41.0
45	35.90781460198057	38.0	35.0	40.0	29.0	41.0
46	35.72342997793445	38.0	34.0	40.0	28.0	41.0
47	35.58967956556352	38.0	34.0	40.0	28.0	41.0
48	35.52205468384237	38.0	34.0	40.0	28.0	41.0
49	35.36309581426221	38.0	34.0	40.0	28.0	41.0
50	35.2792072205903	37.0	34.0	40.0	28.0	41.0
51	34.03081867729185	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	4.0
12	6.0
13	5.0
14	16.0
15	26.0
16	65.0
17	128.0
18	213.0
19	370.0
20	631.0
21	1034.0
22	1506.0
23	1964.0
24	2553.0
25	2993.0
26	3563.0
27	4454.0
28	5417.0
29	7082.0
30	8982.0
31	11797.0
32	15559.0
33	21061.0
34	33069.0
35	40901.0
36	47498.0
37	69218.0
38	104512.0
39	121557.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.6621659795464	31.664556249370328	19.549048929415925	27.124228841667342
2	23.4742275576381	30.47732305583157	19.520010114199586	26.52843927233075
3	20.95515972336084	32.46085192377212	20.792384323780812	25.791604029086223
4	21.67935221712342	30.688496480772155	19.502823876622568	28.129327425481858
5	18.467896305749093	33.59514360385525	18.22314057749709	29.713819512898564
6	20.064636056726435	36.484999575282636	21.80775514154941	21.642609226441518
7	83.0905201108611	6.274557059296471	5.639258897828806	4.995663932013614
8	84.9300401604839	4.940746988951422	4.916844290712122	5.212368559852553
9	78.109474357936	8.508372858387379	8.342634314397523	5.039518469279106
10	37.284653480015564	32.43694922553282	13.93705096015756	16.34134633429405
11	27.18072612841478	30.212615488553375	24.110513433909038	18.49614494912281
12	26.949403321487342	24.839446958727347	27.99815889960669	20.212990820178618
13	27.127982157919796	25.649175554453706	25.889585337571287	21.333256950055212
14	20.837029032888925	28.152044865957226	28.260693494317678	22.75023260683617
15	21.00019951839026	28.87920050412964	29.07476803517845	21.045831942301653
16	22.335392389459898	28.942809337460663	26.349860435898297	22.37193783718114
17	21.779901584097004	28.23995148344886	27.27771972209656	22.702427210357573
18	24.358824935452837	25.9010428292893	26.218296824101824	23.52183541115604
19	23.87365942408325	29.785725150577118	25.336465047736255	21.00415037760337
20	24.43724949083302	27.988676837495234	25.678214369670044	21.895859302001703
21	26.112611340151197	27.874892092157744	25.507537251663802	20.504959316027254
22	22.73442916998374	27.575416963804205	25.54862618748012	24.141527678731933
23	22.28205579008295	28.031741202918102	25.449459621231128	24.23674338576782
24	20.838806919534825	27.727129957587525	25.843162741817277	25.590900381060372
25	21.983568376532688	28.820727787775645	24.004037778115798	25.191666057575873
26	24.66146075117686	28.829617221005137	23.09652541686503	23.412396610952968
27	22.338553076830383	27.342118727270215	24.657114806042443	25.66221338985696
28	22.47861103593504	27.96497168221659	25.906969118108965	23.649448163739407
29	23.628311066949284	29.45523577740069	23.489833451529872	23.426619704120153
30	23.695475673572112	26.85813847366456	24.06527609591896	25.38110975684437
31	23.60539608351326	28.763242786224936	23.3308113682023	24.300549762059504
32	23.755923819532654	27.348440102011185	23.884129200997986	25.011506877458174
33	21.952554131709796	27.912030168760953	25.03639729050075	25.099018409028506
34	20.910119928331415	27.625197789889356	26.16772582617405	25.296956455605184
35	22.717242932406727	26.339390658983564	26.94446474747096	23.998901661138756
36	23.573196580926435	28.934314990152483	25.08538794474328	22.407100484177796
37	22.469919145666204	29.36100778516808	24.04176848360097	24.127304585564747
38	21.961048479017975	29.222135083827354	27.072670128936288	21.74414630821838
39	22.0999211803587	28.26701486905865	26.105697336528262	23.52736661405439
40	23.180678718104218	28.28657162216353	26.353021123268782	22.179728536463468
41	20.219114651958936	25.961688518210497	28.384750473609248	25.43444635622132
42	23.325872794185916	25.653521499588123	25.78172688105346	25.238878825172506
43	21.827114351693634	24.938218439055035	27.432395860289716	25.802271348961614
44	22.77769107836727	24.43507651826581	26.385418168816265	26.401814234550656
45	24.20651931278755	24.99175258139264	26.77714585979586	24.024582246023954
46	21.06815429685571	25.676831568945456	29.06844666043748	24.186567473761357
47	21.32614540347162	27.44958209786673	27.32513003265385	23.899142466007795
48	21.173642237845673	25.249941230969203	29.548871140751338	24.027545390433787
49	22.19335900074869	24.74857719682588	28.67553371169395	24.382530090731482
50	21.149739539606376	24.707488261009562	27.333229294040724	26.809542905343342
51	21.947022928811442	24.025174874905918	28.504263964805748	25.523538231476888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2228.0
1	1804.5
2	1381.0
3	945.0
4	509.0
5	492.0
6	475.0
7	473.0
8	471.0
9	519.0
10	567.0
11	649.0
12	731.0
13	831.5
14	932.0
15	986.5
16	1041.0
17	1036.0
18	1031.0
19	910.5
20	790.0
21	1090.0
22	1390.0
23	1879.0
24	2368.0
25	2475.0
26	2993.0
27	3404.0
28	4896.0
29	6388.0
30	6807.5
31	7227.0
32	9318.5
33	11410.0
34	12243.5
35	13077.0
36	13302.5
37	13528.0
38	15396.5
39	17265.0
40	19620.0
41	21975.0
42	25505.5
43	29036.0
44	32584.5
45	36133.0
46	40381.0
47	44629.0
48	44361.5
49	44094.0
50	41644.0
51	39194.0
52	37312.0
53	35430.0
54	33081.0
55	30732.0
56	28587.0
57	26442.0
58	25295.0
59	24148.0
60	22654.5
61	21161.0
62	19907.0
63	18653.0
64	16522.0
65	14391.0
66	12207.5
67	10024.0
68	8680.5
69	7337.0
70	6068.5
71	4800.0
72	4023.5
73	3247.0
74	3007.0
75	2135.0
76	1503.0
77	1288.5
78	1074.0
79	800.0
80	526.0
81	297.5
82	69.0
83	50.5
84	32.0
85	24.5
86	17.0
87	10.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	506219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.26378624886336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06690856399999	20.240688133855926
2	9.787155413296263	5.140955155184381
3	3.493472418231463	2.7525543857619277
4	1.6741505537329122	1.7587812916662977
5	0.992985359792198	1.3039777618916484
6	0.6421387172033198	1.0118996406448386
7	0.43063809872049413	0.7917130882788384
8	0.3291690579407126	0.6916180621995676
9	0.2875358698587046	0.6796602562365011
>10	3.4594388572144448	22.56046961867043
>50	1.402842498919505	26.46246558090689
>100	0.42751133327374347	14.884441345331522
>500	0.004539943362199045	0.7881506345289632
>1k	0.0015133144540663485	0.9326250448422653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	2805	0.5541080046383087	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1888	0.37296110971733576	No Hit
AAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	766	0.1513179078620123	No Hit
AATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	757	0.14954002121611398	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	663	0.13097098291450934	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGT	636	0.12563732297681438	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	630	0.12445206521288217	No Hit
CCACCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTC	514	0.10153708177685943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9754296065536853E-4	0.0	0.0	0.6285817008053827	0.0
2	1.9754296065536853E-4	0.0	0.0	0.9152165367163224	0.0
3	1.9754296065536853E-4	0.0	0.0	1.2873874745910368	0.0
4	1.9754296065536853E-4	0.0	0.0	2.6982392995916786	0.0
5	1.9754296065536853E-4	0.0	0.0	2.9192898725650362	0.0
6	1.9754296065536853E-4	0.0	0.0	4.036592858031801	0.0
7	1.9754296065536853E-4	0.0	0.0	4.852840371459783	0.0
8	1.9754296065536853E-4	0.0	0.0	5.685879036543472	0.0
9	1.9754296065536853E-4	0.0	0.0	6.728708325843162	0.0
10	1.9754296065536853E-4	0.0	0.0	8.318138987276258	0.0
11	1.9754296065536853E-4	0.0	0.0	9.626268472736108	0.0
12	1.9754296065536853E-4	0.0	0.0	10.31628603430531	0.0
13	1.9754296065536853E-4	0.0	0.0	10.648750837088295	0.0
14	1.9754296065536853E-4	0.0	0.0	10.938348817409066	0.0
15	1.9754296065536853E-4	0.0	0.0	11.202068669883984	0.0
16	1.9754296065536853E-4	0.0	0.0	11.758547190050157	0.0
17	1.9754296065536853E-4	0.0	0.0	12.394042894478476	0.0
18	1.9754296065536853E-4	0.0	0.0	13.400326736056924	0.0
19	1.9754296065536853E-4	0.0	0.0	13.736939941013672	0.0
20	1.9754296065536853E-4	0.0	0.0	14.243637635094693	0.0
21	1.9754296065536853E-4	0.0	0.0	14.665786942015215	0.0
22	1.9754296065536853E-4	0.0	0.0	15.123691524814358	0.0
23	1.9754296065536853E-4	0.0	0.0	15.571323873659423	0.0
24	1.9754296065536853E-4	0.0	0.0	15.873367060501483	0.0
25	1.9754296065536853E-4	0.0	0.0	16.173039731815678	0.0
26	1.9754296065536853E-4	0.0	0.0	16.449402333772536	0.0
27	1.9754296065536853E-4	0.0	0.0	16.81307892433907	0.0
28	1.9754296065536853E-4	0.0	0.0	17.117887712630303	0.0
29	1.9754296065536853E-4	0.0	0.0	17.42348667276416	0.0
30	1.9754296065536853E-4	0.0	0.0	17.8995652079436	0.0
31	1.9754296065536853E-4	0.0	0.0	18.1903484460283	0.0
32	1.9754296065536853E-4	0.0	0.0	18.490416203263806	0.0
33	1.9754296065536853E-4	0.0	0.0	18.805299682548462	0.0
34	1.9754296065536853E-4	0.0	0.0	19.092527147341368	0.0
35	1.9754296065536853E-4	0.0	0.0	19.39950890819981	0.0
36	1.9754296065536853E-4	0.0	0.0	19.680810084173057	0.0
37	1.9754296065536853E-4	0.0	0.0	19.9767294392348	0.0
38	1.9754296065536853E-4	0.0	0.0	20.279957883840787	0.0
39	1.9754296065536853E-4	0.0	0.0	20.60155782378773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	7.02954E-4	45.000004	41
TCACGAT	35	1.2101191E-7	45.000004	11
ATTTACG	20	7.02954E-4	45.000004	45
TCGCATA	20	7.02954E-4	45.000004	29
CGAACCT	20	7.02954E-4	45.000004	45
AGTTTCG	20	7.02954E-4	45.000004	24
GACACGT	20	7.02954E-4	45.000004	33
CGCATAC	20	7.02954E-4	45.000004	30
CGGCACG	40	6.8012014E-9	45.000004	1
ATAGATC	20	7.02954E-4	45.000004	20
TTCCGTA	20	7.02954E-4	45.000004	26
ATGTCGT	20	7.02954E-4	45.000004	10
ATGTCGA	20	7.02954E-4	45.000004	12
GACCCGA	20	7.02954E-4	45.000004	14
GTTACCG	20	7.02954E-4	45.000004	39
CCGTAAG	20	7.02954E-4	45.000004	1
TCTACGT	20	7.02954E-4	45.000004	27
GTTCGAC	20	7.02954E-4	45.000004	11
CTACGTA	20	7.02954E-4	45.000004	34
TGCGATC	20	7.02954E-4	45.000004	20
>>END_MODULE