FastQCFastQC Report
Sat 18 Jun 2016
SRR3553068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612824
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT40400.6592431105831364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17450.28474733365533983No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7620.12434238867929455No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC7600.12401603070375834No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC253.888666E-545.0000048
GGCCGTA351.210683E-745.00000442
GTTAGAT253.888666E-545.00000424
TGACGTC253.888666E-545.00000411
TTATCCG253.888666E-545.0000041
TTATACG253.888666E-545.0000041
TCTACGG253.888666E-545.0000042
CGAATTG502.1827873E-1145.0000041
ACTCGAG253.888666E-545.00000418
CGTAACG351.210683E-745.0000041
TATACGG253.888666E-545.0000042
TAATAGG453.8380676E-1045.02
GTCGCCC207.0307066E-445.044
CTCCGTA207.0307066E-445.043
ACGTTGA207.0307066E-445.035
TAGCGAT207.0307066E-445.045
TAAACCG207.0307066E-445.01
TACGGTA207.0307066E-445.04
TGTGACG207.0307066E-445.013
AACGGAT207.0307066E-445.033