##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612824
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816794055063117	33.0	31.0	34.0	30.0	34.0
2	32.01491782306176	33.0	31.0	34.0	30.0	34.0
3	32.18086922183204	34.0	31.0	34.0	30.0	34.0
4	35.845794224769264	37.0	35.0	37.0	35.0	37.0
5	35.6171331409997	37.0	35.0	37.0	33.0	37.0
6	34.921261243032255	37.0	35.0	37.0	33.0	37.0
7	35.36440642011409	37.0	35.0	37.0	33.0	37.0
8	35.76683354437816	37.0	35.0	37.0	35.0	37.0
9	37.4393316841377	39.0	37.0	39.0	35.0	39.0
10	37.0332591412869	39.0	37.0	39.0	33.0	39.0
11	37.046897641084556	39.0	37.0	39.0	33.0	39.0
12	36.92419520123233	39.0	37.0	39.0	33.0	39.0
13	36.87690756236701	39.0	37.0	39.0	33.0	39.0
14	38.07282841403078	40.0	37.0	41.0	33.0	41.0
15	38.094330509248984	40.0	37.0	41.0	33.0	41.0
16	38.004794198660626	40.0	37.0	41.0	33.0	41.0
17	38.026511690142684	40.0	37.0	41.0	33.0	41.0
18	37.998657036930666	40.0	37.0	41.0	33.0	41.0
19	38.032730441366525	40.0	37.0	41.0	33.0	41.0
20	37.934162173805205	40.0	37.0	41.0	33.0	41.0
21	37.902791013406784	40.0	37.0	41.0	33.0	41.0
22	37.91264049710847	40.0	37.0	41.0	33.0	41.0
23	37.80456705350965	40.0	37.0	41.0	33.0	41.0
24	37.65915009856011	39.0	36.0	41.0	33.0	41.0
25	37.682966071824865	40.0	36.0	41.0	33.0	41.0
26	37.63856343746328	40.0	36.0	41.0	33.0	41.0
27	37.54976632768952	39.0	36.0	41.0	33.0	41.0
28	37.501620367348536	39.0	36.0	41.0	33.0	41.0
29	37.315790504288344	39.0	36.0	41.0	32.0	41.0
30	37.37323277156247	39.0	36.0	41.0	32.0	41.0
31	37.31687890813676	39.0	36.0	41.0	32.0	41.0
32	37.278670548150856	39.0	36.0	41.0	32.0	41.0
33	37.06252202916335	39.0	36.0	41.0	31.0	41.0
34	37.05961907497095	39.0	35.0	41.0	31.0	41.0
35	37.0370236805347	39.0	35.0	41.0	31.0	41.0
36	36.96338263514484	39.0	35.0	41.0	31.0	41.0
37	36.889723640066315	39.0	35.0	41.0	31.0	41.0
38	36.68730826468937	39.0	35.0	40.0	31.0	41.0
39	36.6958017310027	39.0	35.0	40.0	31.0	41.0
40	36.59367452971816	39.0	35.0	40.0	30.0	41.0
41	36.56664393039437	39.0	35.0	40.0	31.0	41.0
42	36.48452573658995	39.0	35.0	40.0	30.0	41.0
43	36.39239977546571	39.0	35.0	40.0	30.0	41.0
44	36.291217380520344	38.0	35.0	40.0	30.0	41.0
45	36.06713673093743	38.0	35.0	40.0	30.0	41.0
46	35.9289861363132	38.0	35.0	40.0	30.0	41.0
47	35.79450217354412	38.0	35.0	40.0	29.0	41.0
48	35.70175450047648	38.0	34.0	40.0	29.0	41.0
49	35.59347545135308	38.0	34.0	40.0	28.0	41.0
50	35.4992248998081	38.0	34.0	40.0	28.0	41.0
51	34.30606340482749	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	2.0
12	8.0
13	12.0
14	19.0
15	28.0
16	56.0
17	110.0
18	221.0
19	387.0
20	665.0
21	1048.0
22	1495.0
23	2106.0
24	2569.0
25	3156.0
26	3762.0
27	4831.0
28	6033.0
29	7812.0
30	10445.0
31	13534.0
32	18291.0
33	25090.0
34	38301.0
35	48489.0
36	58372.0
37	86266.0
38	129427.0
39	150239.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.849052909154995	30.678955132305525	21.353275981358433	27.11871597718105
2	25.38428651619388	29.48432176285524	19.786594519796875	25.344797201154
3	20.847257940289545	31.311110530919155	20.393293996318683	27.448337532472618
4	20.58421341200736	32.60463036695691	19.15900813284075	27.652148088194977
5	18.419807318251245	32.438024620445674	18.414422411654897	30.727745649648185
6	19.706473636802734	38.46063470099082	19.686076263331724	22.14681539887472
7	84.5624518621986	5.371362740362649	5.261706460582484	4.804478936856259
8	86.31711551766902	4.575701996005378	4.43879482526794	4.668387661057661
9	78.54506350926204	8.632005273944884	7.899005260890565	4.923925955902511
10	34.32029424435074	34.59231361696017	13.296966176259414	17.79042596242967
11	27.54379724031696	30.509248985026694	22.788924715742205	19.158029058914142
12	26.338230878686215	26.484276072738666	27.868523425975482	19.308969622599637
13	25.765146273644635	27.3670743965641	25.52918945733196	21.338589872459305
14	21.70443716303539	28.72668172264794	27.780080414605173	21.7888006997115
15	19.833100531310784	29.911361173844362	27.321057922013498	22.934480372831352
16	21.894214325809696	28.713953761602024	26.538288317689908	22.853543594898373
17	21.856356800647493	27.468898084931396	27.632077072699502	23.042668041721605
18	23.720350377922536	27.623265407360027	25.25749644269807	23.39888777201937
19	22.676168035194443	30.915401485581505	24.72634883751289	21.68208164171116
20	25.271856193621662	28.377805046799732	25.08256856781066	21.267770191767944
21	23.837512891140033	28.56415545083091	25.622038301372008	21.976293356657052
22	23.23978825894547	26.628199939950132	24.783787841207264	25.348223959897133
23	22.00550239546754	29.287854261582446	24.567902040390063	24.13874130255995
24	20.046212289335926	29.530664595381385	26.63994882706944	23.783174288213253
25	22.46550396198582	29.062504079474692	25.006363980522956	23.465627978016528
26	24.51731655418195	28.135157891988566	24.079344151012364	23.268181402817124
27	21.16447789251074	26.832663211623565	26.108148505933187	25.894710389932506
28	21.185201623957287	29.20332754591857	27.630118924846286	21.98135190527786
29	21.056942939571556	28.95284779969453	25.24346304975001	24.746746210983904
30	22.49667114864953	25.81377361199953	26.72724958552537	24.962305653825567
31	22.84685325639988	28.943709776379517	23.566635771444982	24.642801195775622
32	22.325822748456325	26.440707283004585	25.102965941281674	26.130504027257416
33	21.76089709280315	26.47954388209339	25.20397373471013	26.555585290393324
34	18.934637024659608	27.698980457684424	27.48603187864705	25.880350639008913
35	21.92799237627769	25.423449473258223	28.321671475007506	24.326886675456574
36	21.55333342036213	28.095015860997613	26.011057008211168	24.340593710429097
37	21.787658446797124	29.12402255786327	22.533549599885124	26.554769395454485
38	21.300895526284872	28.11410780256648	25.852120674125032	24.732875997023616
39	21.987389527825282	24.381388457371123	24.82703027296581	28.804191741837787
40	23.570062530188114	25.339575473545423	25.188961267835463	25.901400728431
41	21.490509510071405	23.845998198503978	28.350064618879156	26.313427672545465
42	23.64006631594063	25.57716407973578	25.77020482226545	25.012564782058146
43	21.634270198295106	24.59042074070206	27.77518504497213	26.0001240160307
44	22.99485659830555	24.16990848922366	25.489210605328772	27.34602430714202
45	24.040344372935785	23.864111066146236	26.891734005195616	25.203810555722363
46	20.523837186533164	24.91482056838505	28.217726459799223	26.34361578528256
47	21.169046904168244	26.392243123637453	26.431732438677336	26.006977533516963
48	21.926034228424474	25.477135360233934	28.00640967063953	24.59042074070206
49	23.226733939924024	25.03475712439461	26.989315039880946	24.749193895800424
50	21.31052308656319	24.434421628395757	26.36417633774134	27.89087894729971
51	21.16235656566975	23.702074331292508	28.491703980261867	26.64386512277587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2149.0
1	1745.0
2	1341.0
3	909.5
4	478.0
5	482.5
6	487.0
7	501.5
8	516.0
9	554.0
10	592.0
11	652.5
12	713.0
13	735.5
14	758.0
15	825.0
16	892.0
17	990.5
18	1089.0
19	1218.0
20	1347.0
21	1492.0
22	1637.0
23	2603.5
24	3570.0
25	3598.0
26	4314.0
27	5002.0
28	6090.0
29	7178.0
30	8407.5
31	9637.0
32	11050.5
33	12464.0
34	13694.5
35	14925.0
36	17185.5
37	19446.0
38	21131.5
39	22817.0
40	23608.5
41	24400.0
42	25647.0
43	26894.0
44	30544.5
45	34195.0
46	38490.5
47	42786.0
48	47357.0
49	51928.0
50	58458.0
51	64988.0
52	59893.0
53	54798.0
54	49213.5
55	43629.0
56	38597.0
57	33565.0
58	31410.0
59	29255.0
60	26535.0
61	23815.0
62	21587.5
63	19360.0
64	17304.0
65	15248.0
66	13310.0
67	11372.0
68	9832.0
69	8292.0
70	6789.0
71	5286.0
72	4591.0
73	3896.0
74	3658.5
75	2801.0
76	2181.0
77	1801.5
78	1422.0
79	1008.0
80	594.0
81	560.0
82	526.0
83	369.0
84	212.0
85	129.5
86	47.0
87	30.5
88	14.0
89	13.5
90	13.0
91	12.5
92	12.0
93	8.5
94	5.0
95	5.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.231826469558545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82371790419093	19.62762783869937
2	10.741753067447684	5.6355160488827165
3	3.853365579383969	3.032424516065106
4	1.8877866514959554	1.980803674143636
5	1.0375522585254324	1.3608445399368845
6	0.692544173077762	1.0900019142407864
7	0.4911501824770111	0.9018636450060278
8	0.37313708591162864	0.7830453829592481
9	0.28196359695973827	0.665677813156236
>10	4.073298781889176	26.808654597165983
>50	1.4867398376183638	27.27529597708882
>100	0.25254906332569727	9.35764600236772
>500	0.0031727269262022276	0.5176683458565515
>1k	0.001269090770480891	0.9629297044309164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	4040	0.6592431105831364	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1745	0.28474733365533983	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	762	0.12434238867929455	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	760	0.12401603070375834	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7264728535435949	0.0
2	0.0	0.0	0.0	0.9870696970092555	0.0
3	0.0	0.0	0.0	1.2845449917105074	0.0
4	0.0	0.0	0.0	2.515567275433077	0.0
5	0.0	0.0	0.0	2.7032231113663956	0.0
6	0.0	0.0	0.0	3.7824889364646292	0.0
7	0.0	0.0	0.0	4.553020116705612	0.0
8	0.0	0.0	0.0	5.222869861493675	0.0
9	0.0	0.0	0.0	6.0970196989674035	0.0
10	0.0	0.0	0.0	7.446346748821847	0.0
11	0.0	0.0	0.0	8.743782880566036	0.0
12	0.0	0.0	0.0	9.404657781026852	0.0
13	0.0	0.0	0.0	9.669170920198948	0.0
14	0.0	0.0	0.0	9.973010195423155	0.0
15	0.0	0.0	0.0	10.25743117110296	0.0
16	0.0	0.0	0.0	10.841775126300536	0.0
17	0.0	0.0	0.0	11.544097489654453	0.0
18	0.0	0.0	0.0	12.482539848308813	0.0
19	0.0	0.0	0.0	12.866173648551623	0.0
20	0.0	0.0	0.0	13.327317467984283	0.0
21	0.0	0.0	0.0	13.807716407973578	0.0
22	0.0	0.0	0.0	14.312755375115858	0.0
23	0.0	0.0	0.0	14.82774826051199	0.0
24	0.0	0.0	0.0	15.213666566583553	0.0
25	0.0	0.0	0.0	15.555852903933266	0.0
26	0.0	0.0	0.0	15.87290967716669	0.0
27	0.0	0.0	0.0	16.256869835385036	0.0
28	0.0	0.0	0.0	16.59628212994269	0.0
29	0.0	0.0	0.0	16.98970666945159	0.0
30	0.0	0.0	0.0	17.51693797893033	0.0
31	0.0	0.0	0.0	17.856350273487983	0.0
32	0.0	0.0	0.0	18.20946960301816	0.0
33	0.0	0.0	0.0	18.55181911935564	0.0
34	0.0	0.0	0.0	18.88959962403561	0.0
35	0.0	0.0	0.0	19.282208268605668	0.0
36	0.0	0.0	0.0	19.61852016239573	0.0
37	0.0	0.0	0.0	19.94961032857721	0.0
38	0.0	0.0	0.0	20.303871911021762	0.0
39	0.0	0.0	0.0	20.729442711120974	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATC	25	3.888666E-5	45.000004	8
GGCCGTA	35	1.210683E-7	45.000004	42
GTTAGAT	25	3.888666E-5	45.000004	24
TGACGTC	25	3.888666E-5	45.000004	11
TTATCCG	25	3.888666E-5	45.000004	1
TTATACG	25	3.888666E-5	45.000004	1
TCTACGG	25	3.888666E-5	45.000004	2
CGAATTG	50	2.1827873E-11	45.000004	1
ACTCGAG	25	3.888666E-5	45.000004	18
CGTAACG	35	1.210683E-7	45.000004	1
TATACGG	25	3.888666E-5	45.000004	2
TAATAGG	45	3.8380676E-10	45.0	2
GTCGCCC	20	7.0307066E-4	45.0	44
CTCCGTA	20	7.0307066E-4	45.0	43
ACGTTGA	20	7.0307066E-4	45.0	35
TAGCGAT	20	7.0307066E-4	45.0	45
TAAACCG	20	7.0307066E-4	45.0	1
TACGGTA	20	7.0307066E-4	45.0	4
TGTGACG	20	7.0307066E-4	45.0	13
AACGGAT	20	7.0307066E-4	45.0	33
>>END_MODULE