##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733043
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900172295486076	33.0	31.0	34.0	30.0	34.0
2	32.094736597989474	34.0	31.0	34.0	30.0	34.0
3	32.24233094102256	34.0	31.0	34.0	30.0	34.0
4	35.88590983066478	37.0	35.0	37.0	35.0	37.0
5	35.67095109018161	37.0	35.0	37.0	33.0	37.0
6	35.69137690421981	37.0	35.0	37.0	33.0	37.0
7	35.77211023091415	37.0	35.0	37.0	35.0	37.0
8	35.82630214052927	37.0	35.0	37.0	35.0	37.0
9	37.5250619677154	39.0	37.0	39.0	35.0	39.0
10	37.07639524557222	39.0	37.0	39.0	33.0	39.0
11	37.05464345202123	39.0	37.0	39.0	33.0	39.0
12	36.96837702563151	39.0	37.0	39.0	33.0	39.0
13	36.91359988431784	39.0	37.0	39.0	33.0	39.0
14	38.114622198152084	40.0	37.0	41.0	33.0	41.0
15	38.14600371328831	40.0	37.0	41.0	33.0	41.0
16	38.05693526846311	40.0	37.0	41.0	33.0	41.0
17	38.0265741573141	40.0	37.0	41.0	33.0	41.0
18	37.97795081598215	40.0	37.0	41.0	33.0	41.0
19	38.01441661676054	40.0	37.0	41.0	33.0	41.0
20	37.93714556990518	40.0	37.0	41.0	33.0	41.0
21	37.93127142609642	40.0	37.0	41.0	33.0	41.0
22	37.88806659363775	40.0	37.0	41.0	33.0	41.0
23	37.79503794456805	40.0	37.0	41.0	33.0	41.0
24	37.661435959418476	40.0	36.0	41.0	33.0	41.0
25	37.689964981590435	40.0	36.0	41.0	33.0	41.0
26	37.662565497521975	40.0	36.0	41.0	33.0	41.0
27	37.57883916768866	40.0	36.0	41.0	33.0	41.0
28	37.53861096825152	40.0	36.0	41.0	33.0	41.0
29	37.38690226903469	39.0	36.0	41.0	32.0	41.0
30	37.40069954968535	39.0	36.0	41.0	32.0	41.0
31	37.39192925926583	39.0	36.0	41.0	32.0	41.0
32	37.287213710519026	39.0	36.0	41.0	32.0	41.0
33	37.26089738255464	39.0	36.0	41.0	32.0	41.0
34	37.17940011704634	39.0	35.0	41.0	32.0	41.0
35	37.109760273271824	39.0	35.0	41.0	31.0	41.0
36	37.07896671818706	39.0	35.0	41.0	31.0	41.0
37	36.99027205771012	39.0	35.0	41.0	31.0	41.0
38	36.96215638100357	39.0	35.0	41.0	31.0	41.0
39	36.914973610006506	39.0	35.0	41.0	31.0	41.0
40	36.77220299491299	39.0	35.0	41.0	31.0	41.0
41	36.72443772057028	39.0	35.0	40.0	31.0	41.0
42	36.65539675025885	39.0	35.0	40.0	31.0	41.0
43	36.587842732281736	39.0	35.0	40.0	31.0	41.0
44	36.52558581147354	39.0	35.0	40.0	31.0	41.0
45	36.36966180701541	38.0	35.0	40.0	30.0	41.0
46	36.23883046424289	38.0	35.0	40.0	30.0	41.0
47	36.13819653144495	38.0	35.0	40.0	30.0	41.0
48	36.05840721485643	38.0	35.0	40.0	30.0	41.0
49	35.9048445998393	38.0	35.0	40.0	29.0	41.0
50	35.85136069780354	38.0	35.0	40.0	29.0	41.0
51	34.56341033199962	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	1.0
10	4.0
11	2.0
12	2.0
13	7.0
14	13.0
15	28.0
16	44.0
17	123.0
18	213.0
19	438.0
20	717.0
21	1140.0
22	1568.0
23	2200.0
24	2817.0
25	3516.0
26	4350.0
27	5415.0
28	6993.0
29	9117.0
30	12012.0
31	15825.0
32	20889.0
33	28707.0
34	46052.0
35	56285.0
36	64968.0
37	95046.0
38	154506.0
39	199991.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.19343612857636	32.8269965063441	19.164632906937246	26.814934458142293
2	23.830116377893248	31.28875113738212	19.704846782521628	25.17628570220301
3	21.618513511485684	30.313364973132543	21.082119329971093	26.98600218541068
4	21.707457816253616	30.604480228308574	19.212924753391	28.475137202046813
5	18.760973094347808	33.83062112318104	18.015996333093693	29.39240944937746
6	20.478471249299155	36.227479151973355	20.1292420772042	23.164807521523294
7	85.03771265805689	5.473621602006976	5.34579826831441	4.142867471621719
8	86.63748238507154	4.041372743481624	4.0221378554873315	5.2990070159595
9	79.54553825628238	7.944417994578763	7.537074905564885	4.9729688435739785
10	36.039632054326965	34.67982096548224	13.15761831161337	16.122928668577423
11	27.45214127957023	28.24786540489439	25.344215823628353	18.95577749190702
12	28.723690151873765	22.288051314861473	29.516685924290936	19.471572608973826
13	25.997656344852892	25.230443507406797	27.074946490178615	21.6969536575617
14	21.138459817500475	27.724567317333364	28.13231965928329	23.004653205882875
15	20.462510384793255	29.357350114522614	28.45535664347112	21.724782857213015
16	23.15266635108718	26.611672166571402	28.684265452367736	21.551396029973684
17	21.97388147762137	26.9629476033466	27.714472411577496	23.348698507454543
18	23.321278560739277	26.739904753200015	25.905847269532618	24.03296941652809
19	23.57310553405462	29.752824868391077	26.39203975755856	20.282029839995744
20	25.48595375714658	28.09794241265519	25.30479112412232	21.111312706075903
21	25.151048437813333	27.209863541429357	26.30800648802321	21.331081532734096
22	22.954860765330274	26.767597535205983	26.047312367760146	24.230229331703597
23	24.05711533975497	27.5948341366059	24.708509596299262	23.63954092733987
24	22.531283976519795	27.563867331111545	25.68948888400817	24.215359808360493
25	23.100691228208987	27.067989190265784	24.753254583973927	25.0780649975513
26	24.11563850960994	27.345326263261498	24.727608066648205	23.811427160480353
27	21.621105446747325	26.53214067933259	27.064715166777393	24.782038707142693
28	21.727784045410704	27.81569430442689	26.443605627500705	24.0129160226617
29	23.207642662163067	28.590000859431164	25.394826770053054	22.807529708352718
30	23.72575687920081	26.546464532094298	25.487181515954727	24.240597072750166
31	23.91264905332975	26.51986309125113	23.886456865422627	25.681030989996493
32	24.01973690492918	28.120314906492528	24.790633018799717	23.06931516977858
33	23.067541740389036	26.568973443576983	26.199690877615637	24.163793938418348
34	21.31921319758868	27.157615583260466	26.34552134049435	25.177649878656506
35	22.030631218086796	28.00204080797443	26.173635107353867	23.793692866584905
36	21.458768448781314	29.36553517324359	26.146351578283948	23.029344799691152
37	21.74961086866664	31.095310916276397	24.114956421383194	23.04012179367377
38	22.6138166519563	28.607053065099862	25.17505794339486	23.604072339548978
39	22.4971795651824	25.835455764532227	26.880687763200793	24.78667690708458
40	22.602357569746932	27.126785195411458	26.434738480552983	23.83611875428863
41	20.524307578136618	25.816902964764687	28.71550509315279	24.94328436394591
42	22.158727387070062	27.833837851258387	25.01572213362654	24.99171262804501
43	21.25987152186161	26.29068144706381	26.888327151340373	25.561119879734207
44	22.757873685445464	25.697810360374497	24.963747010748346	26.580568943431693
45	23.0294812173365	25.966825957003888	26.5957113020655	24.407981523594113
46	20.184081970634736	26.274311329621863	29.07114589457917	24.470460805164226
47	22.02039989468558	26.226565153749508	27.326227792912555	24.42680715865236
48	21.950554060266587	25.20370564891828	28.461495437511854	24.384244853303287
49	21.727101957183958	26.338427622936173	27.216820841342187	24.717649578537685
50	21.07707187709316	24.281795201645743	26.86813733982863	27.77299558143247
51	20.975986401889113	24.03365150475484	29.407551808011263	25.582810285344788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1857.0
1	1474.0
2	1091.0
3	764.5
4	438.0
5	423.5
6	409.0
7	416.0
8	423.0
9	526.0
10	629.0
11	679.0
12	729.0
13	718.5
14	708.0
15	734.0
16	760.0
17	827.5
18	895.0
19	1143.0
20	1391.0
21	1513.0
22	1635.0
23	2337.5
24	3040.0
25	3623.0
26	5441.0
27	6676.0
28	7999.5
29	9323.0
30	11024.0
31	12725.0
32	14641.5
33	16558.0
34	19234.0
35	21910.0
36	22285.0
37	22660.0
38	25291.0
39	27922.0
40	30304.5
41	32687.0
42	38135.0
43	43583.0
44	48065.0
45	52547.0
46	58311.0
47	64075.0
48	61878.5
49	59682.0
50	56297.5
51	52913.0
52	50180.5
53	47448.0
54	45212.0
55	42976.0
56	40349.5
57	37723.0
58	35865.0
59	34007.0
60	34038.5
61	34070.0
62	31252.5
63	28435.0
64	24185.5
65	19936.0
66	17454.0
67	14972.0
68	12824.0
69	10676.0
70	9007.0
71	7338.0
72	6314.0
73	5290.0
74	4369.0
75	2990.5
76	2533.0
77	1856.5
78	1180.0
79	987.0
80	794.0
81	585.0
82	376.0
83	252.5
84	129.0
85	129.0
86	129.0
87	104.0
88	79.0
89	50.0
90	21.0
91	15.0
92	9.0
93	5.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	733043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.23343951177353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78195280203151	18.364573693121073
2	9.893348220525437	4.794997113420309
3	3.4706648036016965	2.5231843675116923
4	1.6017223230117315	1.552609641174487
5	0.953778734130217	1.155666963058027
6	0.6112885039179615	0.8888173790363074
7	0.47661112858929056	0.8084948868714682
8	0.3502830684176267	0.6790850840397589
9	0.3180176698319806	0.6935995768993621
>10	4.486974650908413	27.572115287979965
>50	1.791632511648136	30.1754491793209
>100	0.25578545829481136	8.440489016321592
>500	0.005104366130051667	0.7159269733280006
>1k	0.002835758961139815	1.6349908379170466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	3125	0.4263051417174708	No Hit
AATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	2478	0.3380429251762857	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC	2434	0.3320405487809037	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGT	2351	0.3207178842168877	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1508	0.20571780918718277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0925293604877205E-4	0.0	0.0	0.48960292915968096	0.0
2	4.0925293604877205E-4	0.0	0.0	0.7101902616899691	0.0
3	4.0925293604877205E-4	0.0	0.0	1.0313173988429056	0.0
4	4.0925293604877205E-4	0.0	0.0	2.3924926641411215	0.0
5	4.0925293604877205E-4	0.0	0.0	2.5695627678048902	0.0
6	4.0925293604877205E-4	0.0	0.0	3.4785135387692128	0.0
7	4.0925293604877205E-4	0.0	0.0	4.144231648075215	0.0
8	4.0925293604877205E-4	0.0	0.0	4.66261870040366	0.0
9	4.0925293604877205E-4	0.0	0.0	5.663105711397558	0.0
10	4.0925293604877205E-4	0.0	0.0	6.826748226229566	0.0
11	4.0925293604877205E-4	0.0	0.0	8.081654145800451	0.0
12	4.0925293604877205E-4	0.0	0.0	8.600859704000992	0.0
13	4.0925293604877205E-4	0.0	0.0	8.82826791879876	0.0
14	4.0925293604877205E-4	0.0	0.0	9.110652444672413	0.0
15	4.0925293604877205E-4	0.0	0.0	9.318007265603791	0.0
16	4.0925293604877205E-4	0.0	0.0	9.730943478077002	0.0
17	4.0925293604877205E-4	0.0	0.0	10.25792484206247	0.0
18	4.0925293604877205E-4	0.0	0.0	10.985849397647886	0.0
19	4.0925293604877205E-4	0.0	0.0	11.30165624663219	0.0
20	4.0925293604877205E-4	0.0	0.0	11.684171324192441	0.0
21	4.0925293604877205E-4	0.0	0.0	12.05809209009567	0.0
22	4.0925293604877205E-4	0.0	0.0	12.453021173382735	0.0
23	4.0925293604877205E-4	0.0	0.0	12.837309680332531	0.0
24	4.0925293604877205E-4	0.0	0.0	13.140429688299323	0.0
25	5.456705813983627E-4	0.0	0.0	13.420767949492731	0.0
26	5.456705813983627E-4	0.0	0.0	13.658816740627767	0.0
27	5.456705813983627E-4	0.0	0.0	13.955661536908476	0.0
28	5.456705813983627E-4	0.0	0.0	14.229178915834405	0.0
29	5.456705813983627E-4	0.0	0.0	14.524795953306969	0.0
30	5.456705813983627E-4	0.0	0.0	14.915496089588196	0.0
31	5.456705813983627E-4	0.0	0.0	15.2142507329038	0.0
32	5.456705813983627E-4	0.0	0.0	15.503456141044932	0.0
33	5.456705813983627E-4	0.0	0.0	15.812714943052454	0.0
34	5.456705813983627E-4	0.0	0.0	16.085823069042334	0.0
35	5.456705813983627E-4	0.0	0.0	16.373255047793922	0.0
36	6.820882267479534E-4	0.0	0.0	16.66382463238855	0.0
37	6.820882267479534E-4	0.0	0.0	16.94020678186682	0.0
38	6.820882267479534E-4	0.0	0.0	17.25342169558948	0.0
39	6.820882267479534E-4	0.0	0.0	17.578777779748254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTG	20	7.031612E-4	45.000004	1
CGAAACG	40	6.8084773E-9	45.000004	1
TAACGCG	20	7.031612E-4	45.000004	1
CGCGAAA	20	7.031612E-4	45.000004	14
CGACAAT	20	7.031612E-4	45.000004	22
TTATACG	20	7.031612E-4	45.000004	1
GTTCGTC	20	7.031612E-4	45.000004	42
CGGATTA	20	7.031612E-4	45.000004	13
CTACGTG	20	7.031612E-4	45.000004	14
TAATTCG	20	7.031612E-4	45.000004	15
TCTAACG	20	7.031612E-4	45.000004	1
CTTGACG	20	7.031612E-4	45.000004	1
AGTTCGT	20	7.031612E-4	45.000004	29
CGTACCG	20	7.031612E-4	45.000004	1
TCGTACG	20	7.031612E-4	45.000004	1
CGGTATC	20	7.031612E-4	45.000004	37
TATCGCG	20	7.031612E-4	45.000004	1
CCCGCAT	20	7.031612E-4	45.000004	14
GTCGGAC	20	7.031612E-4	45.000004	20
GGTCGAC	25	3.889418E-5	45.0	26
>>END_MODULE