FastQCFastQC Report
Sat 18 Jun 2016
SRR3553063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524426
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT41560.7924854984306652Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC36670.6992406936345643No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGT33180.6326917429723163No Hit
AATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT32250.6149580684405427No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTC13970.26638648732137615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13640.2600938931326822No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGT9760.18610824024743242No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7990.15235705323534682No Hit
AATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT7350.14015323420272832No Hit
AAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT7240.138055702806497No Hit
AACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG7030.13405132468641906No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT6960.1327165319797264No Hit
AGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT6640.12661462246341715No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC6140.11708038884418392No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTAT5720.10907163260402804No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGT5570.10621136251825805No Hit
AGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCCT5540.10563930850110406No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5520.10525793915633475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG302.163235E-645.0000042
CACTCCG302.163235E-645.0000041
CGTAGCG302.163235E-645.0000041
AGCCGAT207.029775E-445.043
CTATCGC253.887894E-545.042
CGTGATT253.887894E-545.035
GTCTCGA207.029775E-445.043
CCTACCG700.045.01
TTCGTGC207.029775E-445.031
ATATGCG253.887894E-545.01
TCGATTG207.029775E-445.01
CCGTCGG207.029775E-445.045
CGTTAAA207.029775E-445.031
AGACGAC207.029775E-445.025
TCGGAAA207.029775E-445.021
CCATCGA551.8189894E-1245.030
CGGGCTA253.887894E-545.06
TCGCTAG207.029775E-445.010
GATATGT207.029775E-445.040
TTGCGTA207.029775E-445.032