##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524426
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.727660337206775	31.0	31.0	34.0	30.0	34.0
2	31.929395186356132	33.0	31.0	34.0	30.0	34.0
3	32.067895184449284	34.0	31.0	34.0	30.0	34.0
4	35.74902083420731	37.0	35.0	37.0	35.0	37.0
5	35.51955471315305	37.0	35.0	37.0	33.0	37.0
6	35.527523425612	37.0	35.0	37.0	33.0	37.0
7	35.61854294028137	37.0	35.0	37.0	35.0	37.0
8	35.68005590874594	37.0	35.0	37.0	35.0	37.0
9	37.35507202160076	39.0	37.0	39.0	34.0	39.0
10	36.925608188762574	39.0	37.0	39.0	33.0	39.0
11	36.92429246452312	39.0	37.0	39.0	33.0	39.0
12	36.837441316792074	39.0	37.0	39.0	33.0	39.0
13	36.82171936555396	39.0	37.0	39.0	33.0	39.0
14	37.9423464893045	40.0	37.0	41.0	33.0	41.0
15	38.00439909539192	40.0	37.0	41.0	33.0	41.0
16	37.87381251120272	40.0	37.0	41.0	33.0	41.0
17	37.85241959780789	40.0	37.0	41.0	33.0	41.0
18	37.75184868789877	40.0	37.0	41.0	33.0	41.0
19	37.78121794113945	40.0	37.0	41.0	33.0	41.0
20	37.726756491859675	39.0	36.0	41.0	33.0	41.0
21	37.690003928104254	39.0	36.0	41.0	33.0	41.0
22	37.67271836255258	39.0	36.0	41.0	33.0	41.0
23	37.52942073810223	39.0	36.0	41.0	32.0	41.0
24	37.41087779782085	39.0	36.0	41.0	32.0	41.0
25	37.40269551852883	39.0	36.0	41.0	32.0	41.0
26	37.39501473992517	39.0	36.0	41.0	32.0	41.0
27	37.25769507995408	39.0	36.0	41.0	32.0	41.0
28	37.14154141861769	39.0	36.0	41.0	31.0	41.0
29	36.96940464431588	39.0	35.0	41.0	31.0	41.0
30	37.0122343285802	39.0	35.0	41.0	31.0	41.0
31	36.973752254846254	39.0	35.0	41.0	31.0	41.0
32	36.83056141381243	39.0	35.0	41.0	31.0	41.0
33	36.82167550807931	39.0	35.0	41.0	31.0	41.0
34	36.720050111931904	39.0	35.0	41.0	30.0	41.0
35	36.55692318840027	39.0	35.0	40.0	30.0	41.0
36	36.50590359745703	39.0	35.0	40.0	30.0	41.0
37	36.35329484045413	39.0	35.0	40.0	30.0	41.0
38	36.275314343682425	39.0	35.0	40.0	30.0	41.0
39	36.19962396982606	39.0	35.0	40.0	30.0	41.0
40	36.11098610671477	38.0	35.0	40.0	29.0	41.0
41	36.042907864979995	38.0	35.0	40.0	29.0	41.0
42	35.93745542745783	38.0	35.0	40.0	29.0	41.0
43	35.87529985164733	38.0	35.0	40.0	29.0	41.0
44	35.710637916502996	38.0	35.0	40.0	28.0	41.0
45	35.509957553591924	38.0	34.0	40.0	28.0	41.0
46	35.31044227402913	38.0	34.0	40.0	27.0	41.0
47	35.17247047247848	37.0	34.0	40.0	27.0	41.0
48	35.1066308687975	37.0	34.0	40.0	27.0	41.0
49	34.90440977373357	37.0	34.0	40.0	26.0	41.0
50	34.79113926464363	37.0	34.0	40.0	26.0	41.0
51	33.39106184666664	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	3.0
12	10.0
13	9.0
14	26.0
15	28.0
16	64.0
17	118.0
18	225.0
19	459.0
20	853.0
21	1308.0
22	1718.0
23	2266.0
24	2780.0
25	3461.0
26	4130.0
27	4910.0
28	6223.0
29	7991.0
30	10285.0
31	13269.0
32	17467.0
33	23293.0
34	34332.0
35	43210.0
36	51246.0
37	72348.0
38	106330.0
39	116026.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.682281198872673	34.016048022027896	19.832159351367018	26.469511427732417
2	25.152071026226768	32.125028125989175	19.014884845526346	23.708016002257708
3	20.682040936185466	29.226049051725123	22.20256051378078	27.889349498308626
4	23.000194498365833	30.079553645318878	18.188457475411212	28.731794380904073
5	18.057266420810564	34.338877172375135	17.19918539507957	30.404671011734735
6	20.164522735333488	36.947252805924954	20.199799399724654	22.688425059016907
7	81.70742869346677	6.661187660413481	6.600549934595157	5.0308337115246005
8	82.94096783912316	4.305469217773337	5.264803804540583	7.488759138562924
9	77.52552314339869	7.630819219489498	9.254689889517302	5.588967747594513
10	33.90640433540671	33.81315953061061	14.05822747155938	18.2222086624233
11	26.89893330994268	29.254079698565672	23.76293318790449	20.08405380358716
12	26.26280924286744	24.31725353052671	30.907887862157864	18.51204936444799
13	26.98741099792916	25.50445630079363	26.030364627230533	21.477768074046672
14	20.286751610332058	28.565898715929418	29.231960276569048	21.915389397169477
15	18.901808834802242	28.520515763901866	28.55312284287964	24.02455255841625
16	21.34238195665356	28.88377006479465	26.852406249880822	22.921441728670967
17	20.952050432282153	27.597983318904863	26.76888636337634	24.68107988543665
18	23.86952591976752	26.06907361572462	25.922246417988433	24.139154046519433
19	23.375843302963624	29.032122739909923	26.377220046298238	21.21481391082822
20	24.96119566916972	27.722119040627273	24.42403694706212	22.89264834314088
21	23.400441625701244	28.355192152944365	26.154881718297716	22.089484503056674
22	22.75478332500677	27.386895386575038	24.55313047026654	25.305190818151658
23	23.081807538146467	28.055626532628054	23.48205466548188	25.3805112637436
24	19.335997833822123	29.86484270421375	25.892118239751653	24.907041222212474
25	23.18039151376934	28.55445763558634	24.101589166059654	24.16356168458467
26	25.85722294470526	26.56828608802767	23.353151826949848	24.221339140317223
27	21.887358750328932	26.40048357632917	25.67321223585406	26.038945437487843
28	24.300282594684475	27.482619092112138	26.01148684466445	22.205611468538937
29	24.07527468127057	31.157494098309392	22.616536937527886	22.150694282892154
30	24.33327104300702	26.893594139115912	24.874815512579467	23.8983193052976
31	26.52614477543066	27.786379775220908	22.211141324038092	23.476334125310338
32	24.088622608337495	28.06840240567783	23.814608734120736	24.028366251863943
33	24.038663224172716	25.701242882694604	24.1557436130169	26.104350280115785
34	24.025124612433405	26.905225904131374	26.359105002421696	22.710544481013528
35	23.770560574799877	26.055344319312923	27.1163138364612	23.057781269426002
36	25.804784659799473	28.805017295099784	25.151880341554385	20.238317703546354
37	23.49597464656596	29.28020349868237	23.073608097233926	24.150213757517744
38	23.31978200928253	27.968483637348264	26.31105246498076	22.400681888388448
39	23.277068642668365	25.01344326940312	25.558229378406104	26.151258709522413
40	25.922246417988433	24.31439326044094	25.458882664093696	24.304477657476937
41	23.726893784823787	22.5753490482928	29.099434429261706	24.598322737621707
42	26.68117141407939	24.270917155137237	25.444009259647693	23.60390217113568
43	23.642420474957383	23.394911770202086	27.51751438715853	25.445153367682
44	24.88759138562924	23.763505241921642	25.420173675599607	25.928729696849505
45	26.26357198155698	23.88115768478298	26.857364051362826	22.99790628229722
46	21.36736164873595	24.655528139337104	29.727740424769177	24.249369787157768
47	22.82743418518533	27.054913371953337	25.564903341939566	24.55274910092177
48	23.14282663330956	23.745580882717483	29.70390484072109	23.407687643251858
49	23.703439570120473	23.34476170136492	28.186436217883937	24.76536251063067
50	22.084145332229905	23.84225801161651	26.62110574227822	27.45249091387536
51	22.181966569163237	22.343857856017816	29.20926880055527	26.264906774263668
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1720.0
1	1373.5
2	1027.0
3	702.0
4	377.0
5	380.5
6	384.0
7	421.0
8	458.0
9	462.0
10	466.0
11	566.0
12	666.0
13	774.5
14	883.0
15	811.0
16	739.0
17	808.5
18	878.0
19	914.5
20	951.0
21	1119.0
22	1287.0
23	1680.0
24	2073.0
25	2233.0
26	3115.5
27	3838.0
28	4764.5
29	5691.0
30	6307.0
31	6923.0
32	8390.0
33	9857.0
34	10678.0
35	11499.0
36	12155.0
37	12811.0
38	14418.0
39	16025.0
40	17257.0
41	18489.0
42	20047.5
43	21606.0
44	29168.5
45	36731.0
46	41887.0
47	47043.0
48	51493.0
49	55943.0
50	53534.5
51	51126.0
52	45911.0
53	40696.0
54	36532.0
55	32368.0
56	29904.5
57	27441.0
58	25661.5
59	23882.0
60	22243.0
61	20604.0
62	19613.0
63	18622.0
64	16344.0
65	14066.0
66	12601.5
67	11137.0
68	9473.0
69	7809.0
70	6776.5
71	5744.0
72	4674.0
73	3604.0
74	3023.0
75	2198.5
76	1955.0
77	1591.5
78	1228.0
79	824.5
80	421.0
81	338.0
82	255.0
83	190.0
84	125.0
85	77.5
86	30.0
87	22.0
88	14.0
89	16.5
90	19.0
91	18.0
92	17.0
93	33.0
94	49.0
95	30.0
96	11.0
97	6.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	524426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.71747888848328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25028999332996	20.372155131375212
2	9.937231295189651	5.309955346784098
3	3.621512819604125	2.9027307690643434
4	1.7511630769378344	1.8714665015351204
5	1.0290358345629476	1.374662159271416
6	0.6347150985115523	1.017479234681039
7	0.488037279025437	0.9127387989408456
8	0.37144026560700283	0.7939157963750175
9	0.3211006151791418	0.7721099016414943
>10	3.9886636014980272	26.7833457633173
>50	1.4193369683573023	26.039670857384213
>100	0.17310739340795034	6.804497152218854
>500	0.010056031152329064	1.7584569310934755
>1k	0.004309727636712456	3.2868156563175766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	4156	0.7924854984306652	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC	3667	0.6992406936345643	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGT	3318	0.6326917429723163	No Hit
AATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	3225	0.6149580684405427	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTC	1397	0.26638648732137615	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1364	0.2600938931326822	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGT	976	0.18610824024743242	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	799	0.15235705323534682	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	735	0.14015323420272832	No Hit
AAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	724	0.138055702806497	No Hit
AACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	703	0.13405132468641906	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	696	0.1327165319797264	No Hit
AGCCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	664	0.12661462246341715	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	614	0.11708038884418392	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTAT	572	0.10907163260402804	No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGT	557	0.10621136251825805	No Hit
AGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCCT	554	0.10563930850110406	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	552	0.10525793915633475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9168119048254664	0.0
2	0.0	0.0	0.0	1.20722466086731	0.0
3	0.0	0.0	0.0	1.686796611914741	0.0
4	0.0	0.0	0.0	3.8121679703142104	0.0
5	0.0	0.0	0.0	4.0821774664108945	0.0
6	0.0	0.0	0.0	5.459302170372941	0.0
7	0.0	0.0	0.0	6.450481097428426	0.0
8	0.0	0.0	0.0	7.2826290077151015	0.0
9	0.0	0.0	0.0	8.894677228055054	0.0
10	0.0	0.0	0.0	10.543527590165247	0.0
11	0.0	0.0	0.0	12.609214646108317	0.0
12	0.0	0.0	0.0	13.358414723907662	0.0
13	0.0	0.0	0.0	13.718618070042293	0.0
14	0.0	0.0	0.0	14.157383501199407	0.0
15	0.0	0.0	0.0	14.468580886531178	0.0
16	0.0	0.0	0.0	15.157333923184586	0.0
17	0.0	0.0	0.0	15.832739032771068	0.0
18	0.0	0.0	0.0	17.204143196561574	0.0
19	0.0	0.0	0.0	17.57883857779744	0.0
20	0.0	0.0	0.0	18.24680698516092	0.0
21	0.0	0.0	0.0	18.71169621643473	0.0
22	0.0	0.0	0.0	19.20671362594532	0.0
23	0.0	0.0	0.0	19.70344719750737	0.0
24	0.0	0.0	0.0	20.038480166887226	0.0
25	0.0	0.0	0.0	20.344338381392227	0.0
26	0.0	0.0	0.0	20.627505119883452	0.0
27	0.0	0.0	0.0	21.01974349097871	0.0
28	0.0	0.0	0.0	21.35344166765187	0.0
29	0.0	0.0	0.0	21.71269159042458	0.0
30	0.0	0.0	0.0	22.271779049856413	0.0
31	0.0	0.0	0.0	22.567912346069797	0.0
32	0.0	0.0	0.0	22.908665855621194	0.0
33	0.0	0.0	0.0	23.23359253736466	0.0
34	0.0	0.0	0.0	23.536590481783893	0.0
35	0.0	0.0	0.0	23.884590008885905	0.0
36	0.0	0.0	0.0	24.178625773703057	0.0
37	0.0	0.0	0.0	24.473233592537365	0.0
38	0.0	0.0	0.0	24.78462166254152	0.0
39	0.0	0.0	0.0	25.13490940571215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	30	2.163235E-6	45.000004	2
CACTCCG	30	2.163235E-6	45.000004	1
CGTAGCG	30	2.163235E-6	45.000004	1
AGCCGAT	20	7.029775E-4	45.0	43
CTATCGC	25	3.887894E-5	45.0	42
CGTGATT	25	3.887894E-5	45.0	35
GTCTCGA	20	7.029775E-4	45.0	43
CCTACCG	70	0.0	45.0	1
TTCGTGC	20	7.029775E-4	45.0	31
ATATGCG	25	3.887894E-5	45.0	1
TCGATTG	20	7.029775E-4	45.0	1
CCGTCGG	20	7.029775E-4	45.0	45
CGTTAAA	20	7.029775E-4	45.0	31
AGACGAC	20	7.029775E-4	45.0	25
TCGGAAA	20	7.029775E-4	45.0	21
CCATCGA	55	1.8189894E-12	45.0	30
CGGGCTA	25	3.887894E-5	45.0	6
TCGCTAG	20	7.029775E-4	45.0	10
GATATGT	20	7.029775E-4	45.0	40
TTGCGTA	20	7.029775E-4	45.0	32
>>END_MODULE