FastQCFastQC Report
Sat 18 Jun 2016
SRR3553062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499575
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT43450.8697392783866286TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT24090.48220987839663715No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC23910.4786068157934244No Hit
AATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT23250.46539558624831107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20680.41395185908021814No Hit
AAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT11310.22639243356853325No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT8040.1609367962768353No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC7060.14132012210378822TruSeq Adapter, Index 15 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT6360.12730821198018313No Hit
AACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG5480.10969323925336537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG650.045.0000042
ATACGAC302.1630276E-645.0000041
TGCACGA302.1630276E-645.00000426
CGAAGTA302.1630276E-645.00000430
AGCGTGA253.8876286E-545.013
CAGAACA406.8012014E-945.038
CGGAACG207.029454E-445.01
TCGCACA253.8876286E-545.019
AGATCGT207.029454E-445.019
CGAACAG253.8876286E-545.01
GATCGTC207.029454E-445.020
CGTATAC207.029454E-445.042
CAACGTG253.8876286E-545.01
CGACGGT253.8876286E-545.028
TTAGCGT207.029454E-445.039
CTATACG551.8189894E-1245.01
CGTTGAT406.8012014E-945.025
CGGCACG207.029454E-445.01
CCTACGC207.029454E-445.037
CGATTAG207.029454E-445.01