Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553062_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 499575 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT | 4345 | 0.8697392783866286 | TruSeq Adapter, Index 14 (95% over 22bp) |
AATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT | 2409 | 0.48220987839663715 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC | 2391 | 0.4786068157934244 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT | 2325 | 0.46539558624831107 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2068 | 0.41395185908021814 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT | 1131 | 0.22639243356853325 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT | 804 | 0.1609367962768353 | No Hit |
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC | 706 | 0.14132012210378822 | TruSeq Adapter, Index 15 (95% over 21bp) |
AATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT | 636 | 0.12730821198018313 | No Hit |
AACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG | 548 | 0.10969323925336537 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGACGG | 65 | 0.0 | 45.000004 | 2 |
ATACGAC | 30 | 2.1630276E-6 | 45.000004 | 1 |
TGCACGA | 30 | 2.1630276E-6 | 45.000004 | 26 |
CGAAGTA | 30 | 2.1630276E-6 | 45.000004 | 30 |
AGCGTGA | 25 | 3.8876286E-5 | 45.0 | 13 |
CAGAACA | 40 | 6.8012014E-9 | 45.0 | 38 |
CGGAACG | 20 | 7.029454E-4 | 45.0 | 1 |
TCGCACA | 25 | 3.8876286E-5 | 45.0 | 19 |
AGATCGT | 20 | 7.029454E-4 | 45.0 | 19 |
CGAACAG | 25 | 3.8876286E-5 | 45.0 | 1 |
GATCGTC | 20 | 7.029454E-4 | 45.0 | 20 |
CGTATAC | 20 | 7.029454E-4 | 45.0 | 42 |
CAACGTG | 25 | 3.8876286E-5 | 45.0 | 1 |
CGACGGT | 25 | 3.8876286E-5 | 45.0 | 28 |
TTAGCGT | 20 | 7.029454E-4 | 45.0 | 39 |
CTATACG | 55 | 1.8189894E-12 | 45.0 | 1 |
CGTTGAT | 40 | 6.8012014E-9 | 45.0 | 25 |
CGGCACG | 20 | 7.029454E-4 | 45.0 | 1 |
CCTACGC | 20 | 7.029454E-4 | 45.0 | 37 |
CGATTAG | 20 | 7.029454E-4 | 45.0 | 1 |