##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499575
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.682205874993745	31.0	31.0	34.0	30.0	34.0
2	31.87762798378622	33.0	31.0	34.0	30.0	34.0
3	32.056251814041936	34.0	31.0	34.0	30.0	34.0
4	35.729982485112345	37.0	35.0	37.0	33.0	37.0
5	35.48481209027673	37.0	35.0	37.0	33.0	37.0
6	34.82045939048191	37.0	35.0	37.0	32.0	37.0
7	35.2497683030576	37.0	35.0	37.0	33.0	37.0
8	35.65062503127658	37.0	35.0	37.0	33.0	37.0
9	37.347709553120154	39.0	37.0	39.0	34.0	39.0
10	36.90491317619977	39.0	37.0	39.0	33.0	39.0
11	36.91390481909623	39.0	37.0	39.0	33.0	39.0
12	36.799253365360556	39.0	35.0	39.0	33.0	39.0
13	36.768797477856175	39.0	35.0	39.0	33.0	39.0
14	37.911130460891755	40.0	37.0	41.0	33.0	41.0
15	37.91667317219637	40.0	37.0	41.0	33.0	41.0
16	37.82229094730521	40.0	37.0	41.0	33.0	41.0
17	37.78467897713056	40.0	37.0	41.0	33.0	41.0
18	37.73610969323925	39.0	37.0	41.0	33.0	41.0
19	37.751901115948556	40.0	36.0	41.0	33.0	41.0
20	37.691874092979035	39.0	36.0	41.0	33.0	41.0
21	37.675384076464994	39.0	36.0	41.0	33.0	41.0
22	37.67107641495271	39.0	36.0	41.0	33.0	41.0
23	37.55845068308062	39.0	36.0	41.0	32.0	41.0
24	37.429633188209976	39.0	36.0	41.0	32.0	41.0
25	37.501027873692635	39.0	36.0	41.0	32.0	41.0
26	37.428382124806085	39.0	36.0	41.0	32.0	41.0
27	37.35028574288145	39.0	36.0	41.0	32.0	41.0
28	37.345823950357804	39.0	36.0	41.0	32.0	41.0
29	37.13996296852324	39.0	36.0	41.0	31.0	41.0
30	37.16656558074363	39.0	36.0	41.0	31.0	41.0
31	37.083172696792275	39.0	35.0	41.0	31.0	41.0
32	37.04304658960116	39.0	35.0	41.0	31.0	41.0
33	36.819046189260874	39.0	35.0	41.0	31.0	41.0
34	36.77282289946454	39.0	35.0	41.0	31.0	41.0
35	36.756356903367866	39.0	35.0	41.0	31.0	41.0
36	36.69730470900265	39.0	35.0	40.0	31.0	41.0
37	36.63449331932142	39.0	35.0	40.0	30.0	41.0
38	36.43598658860031	39.0	35.0	40.0	30.0	41.0
39	36.4054426262323	39.0	35.0	40.0	30.0	41.0
40	36.309703247760595	39.0	35.0	40.0	30.0	41.0
41	36.29649201821548	39.0	35.0	40.0	30.0	41.0
42	36.25736275834459	39.0	35.0	40.0	30.0	41.0
43	36.164940199169294	38.0	35.0	40.0	30.0	41.0
44	36.03991392683781	38.0	35.0	40.0	30.0	41.0
45	35.78271130460892	38.0	35.0	40.0	29.0	41.0
46	35.59373067107041	38.0	34.0	40.0	28.0	41.0
47	35.50064755041785	38.0	34.0	40.0	28.0	41.0
48	35.421472251413704	38.0	34.0	40.0	28.0	41.0
49	35.352177350748136	38.0	34.0	40.0	28.0	41.0
50	35.27194715508182	38.0	34.0	40.0	27.0	41.0
51	33.92977430816194	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	11.0
13	15.0
14	25.0
15	32.0
16	58.0
17	103.0
18	213.0
19	438.0
20	689.0
21	1079.0
22	1491.0
23	1941.0
24	2399.0
25	3041.0
26	3569.0
27	4295.0
28	5506.0
29	7334.0
30	9416.0
31	12485.0
32	16063.0
33	22113.0
34	33563.0
35	41450.0
36	48070.0
37	67432.0
38	101309.0
39	115384.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.767252164339688	32.87594455286994	19.828854526347396	26.527948756442974
2	22.799179302407047	33.12095280988841	19.798028324075464	24.281839563629084
3	20.494420257218636	30.37581944652955	22.38042335985588	26.749336936395935
4	21.441024871140467	31.25416604113496	20.245408597307712	27.059400490416852
5	18.35600260221188	35.469549116749235	18.071360656558074	28.10308762448081
6	20.359905920032027	37.94505329530101	20.359705749887404	21.33533503477956
7	80.48721413201221	7.236350898263525	6.90126607616474	5.375168893559525
8	81.92643747185107	5.247660511434719	5.3531501776510035	7.4727518390632035
9	75.5966571585848	8.376319871891107	10.257719061202021	5.769303908322073
10	32.612920982835405	35.56142721313116	13.69123755191913	18.134414252114297
11	25.59855877495871	27.458739928939597	27.488164940199166	19.454536355902516
12	26.622228894560372	22.65105339538608	31.087224140519442	19.639493569534103
13	25.64679977981284	24.930390832207376	27.095030776159735	22.327778611820047
14	20.26742731321623	27.231346644647953	30.24470800180153	22.256518040334285
15	17.92263423910324	28.52884952209378	29.756693189210832	23.791823049592153
16	20.81249061702447	28.450783165690837	27.306010108592304	23.43071610869239
17	19.812240404343694	28.038232497622978	28.337086523545015	23.812440574488313
18	21.00585497673022	26.637241655407095	27.395686333383374	24.961217034479308
19	21.891207526397437	31.43812240404344	26.493319321423208	20.177350748135918
20	23.97878196466997	29.71185507681529	25.443026572586696	20.86633638592804
21	22.430465896011608	29.442826402442073	25.42881449231847	22.697893209227843
22	21.534904668968625	27.480358304558877	26.21528299054196	24.76945403593054
23	21.85097332732823	28.99764800080068	25.150978331581847	24.000400340289247
24	18.86103187709553	30.02512135315018	26.75574238102387	24.35810438873042
25	21.89060701596357	27.904518841014863	24.124706000100087	26.080168142921483
26	22.272931992193364	28.019816844317667	26.08877545914027	23.618475704348697
27	18.541560326277338	27.43491968172947	27.315418105389583	26.708101886603615
28	20.464995245959063	28.367312215383077	25.722063754191062	25.445628784466795
29	20.37631987189111	29.884001401191014	26.150227693539506	23.589451033378374
30	22.853425411599858	28.731822048741428	24.003803232747835	24.410949306910872
31	23.12946004103488	30.045939048190963	23.14527348246009	23.679327428314068
32	22.05754891657909	26.359605664815096	26.771956162738324	24.810889255867487
33	22.665065305509682	27.93434419256368	24.47620477405795	24.92438572786869
34	18.71951158484712	29.22444077465846	27.697342741330132	24.35870489916429
35	20.797678026322373	25.72866936896362	28.277235650302757	25.196416954411248
36	20.47960766651654	31.903117650002503	27.61927638492719	19.99799829855377
37	19.76980433368363	32.29645198418656	23.39668718410649	24.53705649802332
38	22.217885202422057	29.6508031827053	27.027973777711058	21.103337837161586
39	22.569584146524548	26.45148376119702	26.814192063253767	24.16474002902467
40	23.5940549467047	27.927338237501875	24.249211830055547	24.229394985737876
41	20.494820597507882	24.99824851123455	28.053645598758948	26.453285292498624
42	22.349997497873193	26.89726267327228	24.911174498323575	25.84156533053095
43	20.134714507331232	25.5006755742381	27.80263223740179	26.561977681028875
44	22.149427012961016	24.596306860831707	26.626832807886704	26.62743331832057
45	24.228394135014764	25.14877645999099	26.456087674523342	24.1667417304709
46	20.03162688285042	24.524045438622828	31.06340389330931	24.380923785217433
47	21.15398088375119	26.69509082720312	27.019966971926134	25.13096131711955
48	21.25586748736426	24.95461141970675	30.027323224741032	23.762197868187958
49	21.82935495170895	23.693739678726917	28.892758845018268	25.584146524545865
50	20.84411749987489	23.052995045788922	27.374067957764098	28.72881949657209
51	20.428964619926937	21.61277085522694	30.87884701996697	27.07941750487915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2420.0
1	1866.5
2	1313.0
3	933.5
4	554.0
5	512.0
6	470.0
7	516.0
8	562.0
9	605.0
10	648.0
11	645.5
12	643.0
13	747.5
14	852.0
15	948.5
16	1045.0
17	1061.5
18	1078.0
19	1004.5
20	931.0
21	1232.5
22	1534.0
23	1922.5
24	2311.0
25	2706.0
26	3462.5
27	3824.0
28	4752.0
29	5680.0
30	6503.5
31	7327.0
32	7990.0
33	8653.0
34	10711.0
35	12769.0
36	13379.0
37	13989.0
38	16602.5
39	19216.0
40	21044.0
41	22872.0
42	29890.0
43	36908.0
44	39844.5
45	42781.0
46	46624.0
47	50467.0
48	47807.0
49	45147.0
50	42212.0
51	39277.0
52	35698.0
53	32119.0
54	30424.5
55	28730.0
56	26824.5
57	24919.0
58	22373.0
59	19827.0
60	19397.5
61	18968.0
62	17064.0
63	15160.0
64	13173.0
65	11186.0
66	9199.0
67	7212.0
68	6204.0
69	5196.0
70	4081.5
71	2967.0
72	2685.0
73	2403.0
74	2042.5
75	1340.0
76	998.0
77	978.5
78	959.0
79	636.0
80	313.0
81	317.0
82	321.0
83	245.0
84	169.0
85	101.0
86	33.0
87	29.5
88	26.0
89	17.0
90	8.0
91	5.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.67442592541164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25428640376181	21.5787346105438
2	10.533568935587084	6.040880843474179
3	4.039252393561283	3.47469730659644
4	1.9493588686823617	2.235869859283065
5	1.0624883374672387	1.523312156465905
6	0.6950266647053583	1.1957694367967828
7	0.5283301701133951	1.0604695028953675
8	0.37524952766517805	0.8608051827664682
9	0.3325435021580055	0.8581944617646023
>10	3.8806840774960674	27.392430453644828
>50	1.1822471309568194	23.241845604334248
>100	0.1598591372988488	6.999669812975781
>500	0.002841940218646201	0.5488419138528656
>1k	0.004262910327969301	2.9884788546056735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	4345	0.8697392783866286	TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT	2409	0.48220987839663715	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCC	2391	0.4786068157934244	No Hit
AATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	2325	0.46539558624831107	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2068	0.41395185908021814	No Hit
AAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	1131	0.22639243356853325	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT	804	0.1609367962768353	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	706	0.14132012210378822	TruSeq Adapter, Index 15 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT	636	0.12730821198018313	No Hit
AACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	548	0.10969323925336537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9880398338587799	0.0
2	0.0	0.0	0.0	1.3469449031676926	0.0
3	0.0	0.0	0.0	1.8603813241255067	0.0
4	0.0	0.0	0.0	4.14352199369464	0.0
5	0.0	0.0	0.0	4.487414302156833	0.0
6	0.0	0.0	0.0	6.09157784116499	0.0
7	0.0	0.0	0.0	7.325026272331482	0.0
8	0.0	0.0	0.0	8.36551068408147	0.0
9	0.0	0.0	0.0	10.071961166991944	0.0
10	0.0	0.0	0.0	12.090076565080318	0.0
11	0.0	0.0	0.0	14.145623780213182	0.0
12	0.0	0.0	0.0	15.02777360756643	0.0
13	0.0	0.0	0.0	15.397688034829605	0.0
14	0.0	0.0	0.0	15.871290597007457	0.0
15	0.0	0.0	0.0	16.2269929440024	0.0
16	0.0	0.0	0.0	16.936596106690686	0.0
17	0.0	0.0	0.0	17.772106290346795	0.0
18	0.0	0.0	0.0	18.97913226242306	0.0
19	0.0	0.0	0.0	19.452534654456286	2.001701446229295E-4
20	0.0	0.0	0.0	19.992793874793573	2.001701446229295E-4
21	0.0	0.0	0.0	20.578491717960265	2.001701446229295E-4
22	0.0	0.0	0.0	21.1575839463544	2.001701446229295E-4
23	0.0	0.0	0.0	21.750287744582895	2.001701446229295E-4
24	2.001701446229295E-4	0.0	0.0	22.163839263373866	2.001701446229295E-4
25	2.001701446229295E-4	0.0	0.0	22.52414552369514	2.001701446229295E-4
26	2.001701446229295E-4	0.0	0.0	22.87664514837612	2.001701446229295E-4
27	2.001701446229295E-4	0.0	0.0	23.265775909523093	2.001701446229295E-4
28	2.001701446229295E-4	0.0	0.0	23.638292548666367	2.001701446229295E-4
29	2.001701446229295E-4	0.0	0.0	24.05524695991593	2.001701446229295E-4
30	2.001701446229295E-4	0.0	0.0	24.61492268428164	2.001701446229295E-4
31	2.001701446229295E-4	0.0	0.0	24.96482009708252	2.001701446229295E-4
32	2.001701446229295E-4	0.0	0.0	25.36455987589451	2.001701446229295E-4
33	2.001701446229295E-4	0.0	0.0	25.75529199819847	2.001701446229295E-4
34	2.001701446229295E-4	0.0	0.0	26.141220037031477	2.001701446229295E-4
35	2.001701446229295E-4	0.0	0.0	26.527348246009108	2.001701446229295E-4
36	2.001701446229295E-4	0.0	0.0	26.876444978231497	2.001701446229295E-4
37	2.001701446229295E-4	0.0	0.0	27.226542561177002	2.001701446229295E-4
38	2.001701446229295E-4	0.0	0.0	27.566431466746735	2.001701446229295E-4
39	2.001701446229295E-4	0.0	0.0	27.97057498874043	2.001701446229295E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGG	65	0.0	45.000004	2
ATACGAC	30	2.1630276E-6	45.000004	1
TGCACGA	30	2.1630276E-6	45.000004	26
CGAAGTA	30	2.1630276E-6	45.000004	30
AGCGTGA	25	3.8876286E-5	45.0	13
CAGAACA	40	6.8012014E-9	45.0	38
CGGAACG	20	7.029454E-4	45.0	1
TCGCACA	25	3.8876286E-5	45.0	19
AGATCGT	20	7.029454E-4	45.0	19
CGAACAG	25	3.8876286E-5	45.0	1
GATCGTC	20	7.029454E-4	45.0	20
CGTATAC	20	7.029454E-4	45.0	42
CAACGTG	25	3.8876286E-5	45.0	1
CGACGGT	25	3.8876286E-5	45.0	28
TTAGCGT	20	7.029454E-4	45.0	39
CTATACG	55	1.8189894E-12	45.0	1
CGTTGAT	40	6.8012014E-9	45.0	25
CGGCACG	20	7.029454E-4	45.0	1
CCTACGC	20	7.029454E-4	45.0	37
CGATTAG	20	7.029454E-4	45.0	1
>>END_MODULE