FastQCFastQC Report
Sat 18 Jun 2016
SRR3553059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539988
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT23900.44260242820210816No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10770.19944887664170313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10740.19889330874019426No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC9890.18315221819744143No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGT9020.16704074905368266No Hit
AATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT9020.16704074905368266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA207.0299587E-445.00000433
TGATATC207.0299587E-445.00000429
CAACGTA207.0299587E-445.00000438
ATAATCG207.0299587E-445.0000041
GATCCGA207.0299587E-445.00000428
ATATGCG207.0299587E-445.0000041
TAACGCG207.0299587E-445.0000041
GTTAACG207.0299587E-445.0000041
CTTGCGA207.0299587E-445.00000441
TACGAAC207.0299587E-445.0000049
ACAGCGT207.0299587E-445.00000414
TTAACGG650.045.0000042
TTCGGTC207.0299587E-445.00000444
AGCCTTA207.0299587E-445.00000413
CCGAAAT207.0299587E-445.00000422
ATACGTT207.0299587E-445.00000419
CACGTCC207.0299587E-445.00000440
GTACGTA207.0299587E-445.00000442
CCGTCAG453.8380676E-1045.030
TATTACG351.2103192E-745.01