##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539988
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81881078838789	33.0	31.0	34.0	30.0	34.0
2	32.02466906672	33.0	31.0	34.0	30.0	34.0
3	32.17020933798529	34.0	31.0	34.0	30.0	34.0
4	35.842459462062116	37.0	35.0	37.0	35.0	37.0
5	35.61454513804011	37.0	35.0	37.0	33.0	37.0
6	35.65230523641266	37.0	35.0	37.0	33.0	37.0
7	35.72307532759987	37.0	35.0	37.0	35.0	37.0
8	35.77894508766862	37.0	35.0	37.0	35.0	37.0
9	37.479688437520835	39.0	37.0	39.0	35.0	39.0
10	37.01709852811544	39.0	37.0	39.0	33.0	39.0
11	37.005479751401886	39.0	37.0	39.0	33.0	39.0
12	36.86326547997363	39.0	37.0	39.0	33.0	39.0
13	36.83932605909761	39.0	37.0	39.0	33.0	39.0
14	38.0205449009978	40.0	37.0	41.0	33.0	41.0
15	38.03703230442158	40.0	37.0	41.0	33.0	41.0
16	37.94878034326689	40.0	37.0	41.0	33.0	41.0
17	37.8927457647207	40.0	37.0	41.0	33.0	41.0
18	37.84012792876879	40.0	37.0	41.0	33.0	41.0
19	37.88610672829767	40.0	37.0	41.0	33.0	41.0
20	37.8048234405209	40.0	37.0	41.0	33.0	41.0
21	37.789260131706634	40.0	36.0	41.0	33.0	41.0
22	37.76988933087402	40.0	36.0	41.0	33.0	41.0
23	37.632660355415304	39.0	36.0	41.0	33.0	41.0
24	37.50099076275769	39.0	36.0	41.0	32.0	41.0
25	37.52090787202678	39.0	36.0	41.0	32.0	41.0
26	37.47472166048134	39.0	36.0	41.0	32.0	41.0
27	37.40631458476855	39.0	36.0	41.0	32.0	41.0
28	37.28704711956562	39.0	36.0	41.0	32.0	41.0
29	37.13783454447136	39.0	35.0	41.0	31.0	41.0
30	37.120800832611096	39.0	35.0	41.0	31.0	41.0
31	37.04923813121773	39.0	35.0	41.0	31.0	41.0
32	37.00994466543701	39.0	35.0	41.0	31.0	41.0
33	36.88436594887294	39.0	35.0	41.0	31.0	41.0
34	36.82235160781351	39.0	35.0	41.0	31.0	41.0
35	36.6851244842478	39.0	35.0	41.0	30.0	41.0
36	36.58180366971118	39.0	35.0	40.0	30.0	41.0
37	36.49850367045194	39.0	35.0	40.0	30.0	41.0
38	36.396727334681515	39.0	35.0	40.0	30.0	41.0
39	36.3305610495048	39.0	35.0	40.0	30.0	41.0
40	36.22021044912109	38.0	35.0	40.0	30.0	41.0
41	36.152697837729725	38.0	35.0	40.0	30.0	41.0
42	36.07650170003778	38.0	35.0	40.0	30.0	41.0
43	35.97194011718779	38.0	35.0	40.0	29.0	41.0
44	35.880649199611845	38.0	35.0	40.0	29.0	41.0
45	35.638930865130334	38.0	34.0	40.0	28.0	41.0
46	35.47975325377601	38.0	34.0	40.0	28.0	41.0
47	35.30506233471855	37.0	34.0	40.0	27.0	41.0
48	35.249024052386346	37.0	34.0	40.0	27.0	41.0
49	35.047802914138835	37.0	34.0	40.0	27.0	41.0
50	34.97300125187967	37.0	34.0	40.0	27.0	41.0
51	33.68353000437047	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	7.0
12	5.0
13	8.0
14	16.0
15	33.0
16	47.0
17	105.0
18	198.0
19	408.0
20	669.0
21	1035.0
22	1405.0
23	1982.0
24	2636.0
25	3243.0
26	4029.0
27	5017.0
28	6146.0
29	7881.0
30	10260.0
31	13151.0
32	17212.0
33	23397.0
34	36576.0
35	43884.0
36	51397.0
37	73448.0
38	109758.0
39	125999.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.874537952695245	31.754409357244977	20.506381623295333	26.864671066764444
2	25.887612317310754	29.088794565805166	20.017111491366474	25.0064816255176
3	21.703445261746555	30.08214997370312	20.964910331340697	27.249494433209627
4	22.01197063638451	31.54236760816907	19.822662725838352	26.622999029608064
5	19.008755750127783	34.47169196352512	17.67631873300888	28.843233553338223
6	20.711386178952125	36.927857656096066	19.53061919894516	22.830136966006652
7	85.49689993110958	5.561419883404817	5.0164077720245634	3.92527241346104
8	86.81507737208976	4.319355244931368	4.164536989710883	4.701030393267999
9	80.06066801484477	7.286458217590021	7.626836151914487	5.0260376156507185
10	37.589168648192185	32.40109039460136	12.855100483714452	17.154640473492
11	30.033815566271844	28.906197915509235	22.192530204374915	18.86745631384401
12	28.568227442091302	24.664251798188108	27.53301925227968	19.234501507440907
13	27.368200774832037	23.986273769046722	26.462069527470984	22.183455928650268
14	22.401979303243778	27.345792869471175	27.34857070897872	22.903657118306334
15	21.029171018615227	28.254701956339773	28.401186693037623	22.314940332007378
16	23.803491929450285	26.256139025311676	27.020600457787953	22.919768587450093
17	22.10030593272443	26.73781639591991	27.96747335125966	23.194404320096
18	24.511285435972653	25.470936391179062	26.70096372511982	23.31681444772847
19	24.32942954287873	29.598250331488845	24.721475292043525	21.350844833588894
20	26.277991362771026	28.003214886256732	23.690341266842967	22.028452484129275
21	26.198915531456258	27.122084194463582	25.114817366311843	21.56418290776832
22	24.102017081861078	25.880760313192148	24.429802143751342	25.587420461195432
23	23.856456069394135	27.997474017941137	24.30831796262139	23.837751950043334
24	22.46457328681378	27.709134277058006	25.69908960939873	24.127202826729484
25	25.223153107106082	27.45709163907346	22.982918138921608	24.33683711489885
26	26.380771424550176	27.211530626606518	23.012733616302587	23.394964332540724
27	23.09551323362741	24.703141551293733	25.92650207041638	26.274843144662473
28	23.650710756535332	27.2169011163211	25.772980140299413	23.359407986844154
29	25.49389986444143	28.351000392601318	23.193478373593486	22.961621369363762
30	25.930391045726942	26.145951391512405	24.777402460795425	23.14625510196523
31	26.12409905405305	27.44764698474781	22.139936443032067	24.288317518167073
32	27.10393564301429	25.352044860256157	23.8359000570383	23.708119439691252
33	25.269080053630823	26.650777424683515	23.482929250279636	24.597213271406034
34	24.879441765372565	25.908538708267592	25.07889064201427	24.133128884345577
35	25.415935168929682	26.20613791417587	25.49556656814596	22.88236034874849
36	24.65869611917302	29.328429520656012	23.552745616569258	22.46012874360171
37	24.255724201278547	29.040830536974894	22.652910805425304	24.05053445632125
38	24.264798477003193	28.576190582012934	24.667029637695652	22.49198130328822
39	24.567953361926563	25.66112580279562	23.845344711363957	25.925576123913864
40	24.73166070357119	25.320377489870143	25.083705563827348	24.86425624273132
41	22.706615702571167	24.69888219738216	27.146529182129974	25.447972917916694
42	23.954791587961214	25.590939057904986	25.509085387082674	24.94518396705112
43	22.679948443298738	24.55850870760091	26.298362185826353	26.463180663274
44	24.892405016407775	22.504759365022927	25.34241501663	27.260420601939302
45	25.67594094683586	24.13146218064105	26.207989807180898	23.984607065342193
46	24.207945361748777	24.412949917405573	26.883930754016756	24.495173966828894
47	22.812914361059875	27.180418824122015	25.313525485751537	24.693141329066574
48	22.70402305236413	24.94166537034156	27.369682289236057	24.98462928805825
49	23.38181589220501	25.21722704948999	28.115439602361537	23.285517455943467
50	23.005881612183973	24.53610080224005	26.451513737342314	26.006503848233663
51	22.89310132817766	23.58163514744772	28.247479573620154	25.27778395075446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1315.0
1	1096.5
2	878.0
3	607.5
4	337.0
5	348.0
6	359.0
7	368.0
8	377.0
9	415.5
10	454.0
11	473.0
12	492.0
13	570.0
14	648.0
15	719.5
16	791.0
17	860.0
18	929.0
19	1150.0
20	1371.0
21	1160.5
22	950.0
23	1521.5
24	2093.0
25	2186.0
26	3358.5
27	4438.0
28	4861.5
29	5285.0
30	6606.0
31	7927.0
32	8548.5
33	9170.0
34	10951.5
35	12733.0
36	14134.0
37	15535.0
38	16670.5
39	17806.0
40	19018.5
41	20231.0
42	20912.5
43	21594.0
44	24127.5
45	26661.0
46	31636.0
47	36611.0
48	39829.0
49	43047.0
50	46944.0
51	50841.0
52	47496.5
53	44152.0
54	40608.0
55	37064.0
56	34957.5
57	32851.0
58	31328.0
59	29805.0
60	28694.5
61	27584.0
62	26136.0
63	24688.0
64	21188.5
65	17689.0
66	15677.0
67	13665.0
68	11256.5
69	8848.0
70	7545.5
71	6243.0
72	5373.5
73	4504.0
74	3627.0
75	2499.5
76	2249.0
77	1894.5
78	1540.0
79	1040.5
80	541.0
81	428.5
82	316.0
83	209.5
84	103.0
85	134.5
86	166.0
87	96.5
88	27.0
89	20.0
90	13.0
91	16.0
92	19.0
93	16.5
94	14.0
95	9.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.424465618255983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91080458475538	19.55416106837518
2	9.26721387808419	4.712279212407524
3	3.2243032363419353	2.4592856032562107
4	1.5231048861482608	1.5489651124349662
5	0.8965066496163222	1.1396601244854025
6	0.5684171214742578	0.8671020937050182
7	0.4198815251086207	0.747268437920551
8	0.3208599321906762	0.6526153851406236
9	0.28420362120856935	0.6503152676401008
>10	4.719162077957977	30.38379109501621
>50	1.6648520114682726	29.018776901654242
>100	0.19629579369709504	6.900036838458541
>500	0.002197340974221213	0.5201145086717596
>1k	0.002197340974221213	0.845628350833677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	2390	0.44260242820210816	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1077	0.19944887664170313	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1074	0.19889330874019426	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC	989	0.18315221819744143	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGT	902	0.16704074905368266	No Hit
AATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	902	0.16704074905368266	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8518930050297414E-4	0.0	0.0	0.5340859426505774	0.0
2	1.8518930050297414E-4	0.0	0.0	0.7261272472721616	0.0
3	1.8518930050297414E-4	0.0	0.0	0.9813181033652599	0.0
4	1.8518930050297414E-4	0.0	0.0	2.0444898775528344	0.0
5	1.8518930050297414E-4	0.0	0.0	2.2024563508818713	0.0
6	1.8518930050297414E-4	0.0	0.0	3.099698511818781	0.0
7	1.8518930050297414E-4	0.0	0.0	3.745638791973155	0.0
8	1.8518930050297414E-4	0.0	0.0	4.422135306710519	0.0
9	1.8518930050297414E-4	0.0	0.0	5.301599294799144	0.0
10	1.8518930050297414E-4	0.0	0.0	6.509218723379038	0.0
11	1.8518930050297414E-4	0.0	0.0	7.57220530826611	0.0
12	1.8518930050297414E-4	0.0	0.0	8.047215864056238	0.0
13	1.8518930050297414E-4	0.0	0.0	8.260553938235665	0.0
14	1.8518930050297414E-4	0.0	0.0	8.5055593828011	0.0
15	1.8518930050297414E-4	0.0	0.0	8.707230531048838	0.0
16	1.8518930050297414E-4	0.0	0.0	9.109832070342303	0.0
17	1.8518930050297414E-4	0.0	0.0	9.591324251650036	0.0
18	1.8518930050297414E-4	0.0	0.0	10.281524774624621	0.0
19	1.8518930050297414E-4	0.0	0.0	10.553753046363994	0.0
20	1.8518930050297414E-4	0.0	0.0	10.887649355170856	0.0
21	1.8518930050297414E-4	0.0	0.0	11.225249449987777	0.0
22	1.8518930050297414E-4	0.0	0.0	11.58284998925902	0.0
23	1.8518930050297414E-4	0.0	0.0	11.952487833062957	0.0
24	1.8518930050297414E-4	0.0	0.0	12.228975458713897	0.0
25	1.8518930050297414E-4	0.0	0.0	12.481573664599955	0.0
26	1.8518930050297414E-4	0.0	0.0	12.697689578286925	0.0
27	1.8518930050297414E-4	0.0	0.0	12.962139899405171	0.0
28	1.8518930050297414E-4	0.0	0.0	13.208256479773624	0.0
29	1.8518930050297414E-4	0.0	0.0	13.454373060142077	0.0
30	1.8518930050297414E-4	0.0	0.0	13.82067749653696	0.0
31	1.8518930050297414E-4	0.0	0.0	14.064201426698371	0.0
32	3.703786010059483E-4	0.0	0.0	14.318466336288955	0.0
33	3.703786010059483E-4	0.0	0.0	14.588101957821285	0.0
34	3.703786010059483E-4	0.0	0.0	14.830699941480182	0.0
35	3.703786010059483E-4	0.0	0.0	15.112372867545204	0.0
36	3.703786010059483E-4	0.0	0.0	15.369045238042327	0.0
37	3.703786010059483E-4	0.0	0.0	15.618680415120336	0.0
38	3.703786010059483E-4	0.0	0.0	15.887019711549145	0.0
39	3.703786010059483E-4	0.0	0.0	16.186100431861448	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	7.0299587E-4	45.000004	33
TGATATC	20	7.0299587E-4	45.000004	29
CAACGTA	20	7.0299587E-4	45.000004	38
ATAATCG	20	7.0299587E-4	45.000004	1
GATCCGA	20	7.0299587E-4	45.000004	28
ATATGCG	20	7.0299587E-4	45.000004	1
TAACGCG	20	7.0299587E-4	45.000004	1
GTTAACG	20	7.0299587E-4	45.000004	1
CTTGCGA	20	7.0299587E-4	45.000004	41
TACGAAC	20	7.0299587E-4	45.000004	9
ACAGCGT	20	7.0299587E-4	45.000004	14
TTAACGG	65	0.0	45.000004	2
TTCGGTC	20	7.0299587E-4	45.000004	44
AGCCTTA	20	7.0299587E-4	45.000004	13
CCGAAAT	20	7.0299587E-4	45.000004	22
ATACGTT	20	7.0299587E-4	45.000004	19
CACGTCC	20	7.0299587E-4	45.000004	40
GTACGTA	20	7.0299587E-4	45.000004	42
CCGTCAG	45	3.8380676E-10	45.0	30
TATTACG	35	1.2103192E-7	45.0	1
>>END_MODULE