##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746583
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675304688159255	31.0	31.0	34.0	30.0	34.0
2	31.883957979219993	33.0	31.0	34.0	30.0	34.0
3	32.06435185371218	34.0	31.0	34.0	30.0	34.0
4	35.746385867344955	37.0	35.0	37.0	33.0	37.0
5	35.5119323638497	37.0	35.0	37.0	33.0	37.0
6	34.84520810144351	37.0	35.0	37.0	32.0	37.0
7	35.29667699371671	37.0	35.0	37.0	33.0	37.0
8	35.68409674476917	37.0	35.0	37.0	33.0	37.0
9	37.361511044317915	39.0	37.0	39.0	34.0	39.0
10	36.90668418648697	39.0	37.0	39.0	33.0	39.0
11	36.8924633965681	39.0	37.0	39.0	33.0	39.0
12	36.73479438990708	39.0	35.0	39.0	33.0	39.0
13	36.670715781098686	39.0	35.0	39.0	33.0	39.0
14	37.8069149712758	40.0	37.0	41.0	33.0	41.0
15	37.80518575965432	40.0	36.0	41.0	33.0	41.0
16	37.71599540841407	40.0	36.0	41.0	33.0	41.0
17	37.68240637678597	39.0	36.0	41.0	33.0	41.0
18	37.71347057192569	39.0	36.0	41.0	33.0	41.0
19	37.736914716783	39.0	36.0	41.0	33.0	41.0
20	37.64021950673937	39.0	36.0	41.0	33.0	41.0
21	37.63103231656761	39.0	36.0	41.0	33.0	41.0
22	37.621137904291956	39.0	36.0	41.0	33.0	41.0
23	37.53466124998828	39.0	36.0	41.0	32.0	41.0
24	37.36036582670648	39.0	36.0	41.0	32.0	41.0
25	37.43917822934623	39.0	36.0	41.0	32.0	41.0
26	37.36652723139959	39.0	36.0	41.0	32.0	41.0
27	37.2544847659269	39.0	36.0	41.0	32.0	41.0
28	37.232996197341755	39.0	35.0	41.0	32.0	41.0
29	37.048987185617676	39.0	35.0	41.0	31.0	41.0
30	37.02425852182544	39.0	35.0	41.0	31.0	41.0
31	37.046343139342845	39.0	35.0	41.0	31.0	41.0
32	36.9871266824988	39.0	35.0	41.0	31.0	41.0
33	36.81818230524938	39.0	35.0	41.0	31.0	41.0
34	36.78731500717268	39.0	35.0	41.0	31.0	41.0
35	36.771561902695346	39.0	35.0	40.0	31.0	41.0
36	36.66269791838282	39.0	35.0	40.0	30.0	41.0
37	36.61124349201629	39.0	35.0	40.0	30.0	41.0
38	36.42963340981512	39.0	35.0	40.0	30.0	41.0
39	36.40645045493937	39.0	35.0	40.0	30.0	41.0
40	36.30697591560483	39.0	35.0	40.0	30.0	41.0
41	36.28174898169393	39.0	35.0	40.0	30.0	41.0
42	36.13696132914894	38.0	35.0	40.0	30.0	41.0
43	36.02077063099481	38.0	35.0	40.0	30.0	41.0
44	35.89185797158521	38.0	35.0	40.0	29.0	41.0
45	35.602246501728544	38.0	34.0	40.0	28.0	41.0
46	35.443414864790654	38.0	34.0	40.0	27.0	41.0
47	35.34849976492902	38.0	34.0	40.0	27.0	41.0
48	35.21914107339706	38.0	34.0	40.0	27.0	41.0
49	35.14719060037531	37.0	34.0	40.0	27.0	41.0
50	35.071583467611774	37.0	34.0	40.0	26.0	41.0
51	33.739249353387365	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	5.0
11	6.0
12	2.0
13	10.0
14	22.0
15	48.0
16	65.0
17	157.0
18	273.0
19	511.0
20	853.0
21	1337.0
22	1922.0
23	2612.0
24	3468.0
25	4440.0
26	5722.0
27	7219.0
28	9180.0
29	11829.0
30	15232.0
31	19539.0
32	25246.0
33	34387.0
34	53010.0
35	62294.0
36	69382.0
37	99553.0
38	148472.0
39	169735.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.65704683873059	31.336904269183734	21.17192596134656	26.834122930739113
2	22.92484559653783	30.77246602186227	20.165607842664514	26.137080538935393
3	21.184784545053933	30.258256617147726	21.245594930503373	27.311363907294968
4	20.656778951570022	31.30743668152101	19.681535743514118	28.354248623394852
5	18.3987580751236	33.146080208094745	18.38603343499651	30.069128281785147
6	20.59101265365003	37.459331380435934	20.569983511545267	21.37967245436877
7	82.3759715932455	5.506822416261822	6.569262895083333	5.547943095409352
8	83.94337936974188	4.957117962771721	5.308184086699001	5.791318580787401
9	76.30189811447622	7.99763723524377	10.128009879678482	5.572454770601527
10	36.18941229575278	30.384565413356583	14.626906854294832	18.799115436595798
11	28.065064433559293	27.873793000912155	25.045708246772296	19.015434318756252
12	28.67477561101713	22.26343219708994	29.646670229565903	19.415121962327028
13	26.43952514321917	22.90997785912618	27.391462168305463	23.259034829349183
14	21.369894573007958	26.426934446672373	30.33848882173851	21.86468215858116
15	20.84336235890718	28.526901898382363	28.251112066575317	22.37862367613514
16	23.097498871525335	26.191193745370573	26.75000636232006	23.961301020784028
17	20.538640713758554	26.90002317223939	29.325205636881634	23.23613047712043
18	23.05276171571011	26.27142594996136	26.975835238680762	23.69997709564777
19	22.109263136181777	30.995749970197554	26.32286028479084	20.57212660882983
20	25.352974819946343	26.994453396340397	26.531008608553908	21.12156317515936
21	23.84115362926828	28.831757487111283	24.623919912454475	22.703168971165965
22	21.325827135094155	25.93375418406259	27.608048937626496	25.132369743216763
23	24.6470921518438	26.389564187772823	24.747549837057633	24.215793823325736
24	21.18625792443707	27.348332335453662	26.1882469866043	25.277162753504967
25	22.4509532094891	25.798471167974625	24.336209102002055	27.414366520534223
26	23.281403407256796	26.645262482537106	26.482788919651263	23.590545190554835
27	20.548686482279933	26.69415188934117	25.284663594000932	27.472498034377956
28	20.898547113984648	27.050575756479855	26.206597257103365	25.844279872432136
29	23.609163348214466	26.88046740951776	24.818807821769315	24.691561420498456
30	22.41090407898385	26.084976486204482	25.411240277370368	26.092879157441303
31	23.148129544873107	25.843208323789852	24.067652223530406	26.941009907806635
32	23.25488257836034	25.699218974983356	24.168243852324526	26.877654594331773
33	21.69725268322477	24.70683098865096	25.10196455049204	28.493951777632226
34	18.929978314534353	24.394206672265508	28.274150362384354	28.401664650815782
35	20.43336105965445	24.95449266859813	26.288838615398426	28.323307656348994
36	20.086849017456867	28.640486054464137	27.001016631774366	24.27164829630463
37	21.48053197032346	27.899242281166327	23.561077602892112	27.0591481456181
38	21.325961078674442	26.055910729282612	25.480489108377768	27.137639083665178
39	21.891738761798756	23.413605720998202	25.920627713194648	28.774027804008394
40	21.27934871273522	23.915894147067373	26.078547194350794	28.72620994584661
41	19.68876869684951	23.295467483186734	29.37851518183511	27.637248638128646
42	22.134578472855665	24.69450817926473	25.644302107066462	27.526611240813143
43	20.107476328820777	23.7950770376502	27.416911448559638	28.680535184969386
44	22.408894925279572	22.77375723797622	25.53406654049181	29.283281296252394
45	23.550228172889014	23.37529785703666	26.95467215299572	26.11980181707861
46	19.963085149273425	24.042202943276234	29.16969714017062	26.825014767279725
47	20.57949350574551	24.16810990874424	27.69444254691039	27.557954038599863
48	20.969001437214615	22.579806933723376	29.999477620036892	26.451714009025117
49	20.957348345729812	22.25057361338257	28.424970833785395	28.367107207102226
50	20.36839842321617	21.27988448705636	27.91169903413284	30.44001805559462
51	20.45010400719009	20.725358064675998	30.834615843114566	27.989922085019348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1869.0
1	1527.0
2	1185.0
3	824.5
4	464.0
5	450.5
6	437.0
7	453.0
8	469.0
9	515.5
10	562.0
11	611.0
12	660.0
13	797.0
14	934.0
15	983.5
16	1033.0
17	1028.5
18	1024.0
19	1275.5
20	1527.0
21	1719.0
22	1911.0
23	2353.0
24	2795.0
25	3442.0
26	4823.5
27	5558.0
28	7067.5
29	8577.0
30	9723.5
31	10870.0
32	12064.0
33	13258.0
34	14766.0
35	16274.0
36	18556.5
37	20839.0
38	22191.5
39	23544.0
40	25430.5
41	27317.0
42	29576.0
43	31835.0
44	35459.0
45	39083.0
46	46081.5
47	53080.0
48	58935.0
49	64790.0
50	72008.0
51	79226.0
52	73304.5
53	67383.0
54	60607.5
55	53832.0
56	48993.0
57	44154.0
58	41995.0
59	39836.0
60	37873.0
61	35910.0
62	31373.0
63	26836.0
64	23628.0
65	20420.0
66	17062.5
67	13705.0
68	12114.5
69	10524.0
70	8889.5
71	7255.0
72	6172.5
73	5090.0
74	4199.0
75	2610.0
76	1912.0
77	1578.5
78	1245.0
79	1178.5
80	1112.0
81	822.0
82	532.0
83	373.0
84	214.0
85	128.0
86	42.0
87	35.0
88	28.0
89	23.0
90	18.0
91	14.0
92	10.0
93	6.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	746583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.953419842772508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33914999030617	21.059868902766386
2	10.465921441046733	5.851165921661079
3	3.9249151661302597	3.2914440445831303
4	1.887769755507308	2.1107848216873517
5	1.064574097343417	1.4879243348390552
6	0.700976492934309	1.1756814124144197
7	0.4815658686302557	0.9422989035439677
8	0.38186377848520353	0.8539518818195501
9	0.300821599938591	0.7568093232772184
>10	3.9437430109692806	27.053528553561907
>50	1.3023224962597657	25.441909247703688
>100	0.2005077820000236	7.933773461244827
>500	0.004401390336585883	0.9323236440431752
>1k	9.780867414635294E-4	0.3673245794052803
>5k	4.890433707317647E-4	0.7412109674489876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	5422	0.726242092305879	TruSeq Adapter, Index 16 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1541	0.20640705721935806	No Hit
AAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	1146	0.1534993430067387	TruSeq Adapter, Index 16 (100% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	930	0.12456752966515444	TruSeq Adapter, Index 16 (100% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	922	0.12349598102287355	TruSeq Adapter, Index 16 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	796	0.1066190899069494	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGT	752	0.10072557237440447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.678871605702246E-4	0.0	0.0	0.9203263401390066	0.0
2	2.678871605702246E-4	0.0	0.0	1.2601412033223365	0.0
3	2.678871605702246E-4	0.0	0.0	1.707914592215467	0.0
4	2.678871605702246E-4	0.0	0.0	3.564104727806553	0.0
5	2.678871605702246E-4	0.0	0.0	3.8302506218330716	0.0
6	2.678871605702246E-4	0.0	0.0	5.245498491125568	0.0
7	2.678871605702246E-4	0.0	0.0	6.320395722913594	0.0
8	2.678871605702246E-4	0.0	0.0	7.210450813908166	0.0
9	2.678871605702246E-4	0.0	0.0	8.444473019074906	0.0
10	2.678871605702246E-4	0.0	0.0	10.368974380611398	0.0
11	2.678871605702246E-4	0.0	0.0	11.95674158131112	0.0
12	2.678871605702246E-4	0.0	0.0	12.760938837342934	0.0
13	2.678871605702246E-4	0.0	0.0	13.086689624596328	0.0
14	2.678871605702246E-4	0.0	0.0	13.488788252612235	0.0
15	4.018307408553369E-4	0.0	0.0	13.810252845296505	0.0
16	4.018307408553369E-4	0.0	0.0	14.436439083129404	0.0
17	4.018307408553369E-4	0.0	0.0	15.210365090016783	0.0
18	4.018307408553369E-4	0.0	0.0	16.16551676102992	0.0
19	4.018307408553369E-4	0.0	0.0	16.603512268562238	0.0
20	5.357743211404492E-4	0.0	0.0	17.099371402777724	0.0
21	5.357743211404492E-4	0.0	0.0	17.616795453419112	0.0
22	5.357743211404492E-4	0.0	0.0	18.198512422597354	0.0
23	5.357743211404492E-4	0.0	0.0	18.739510543368922	0.0
24	5.357743211404492E-4	0.0	0.0	19.194918716338304	0.0
25	5.357743211404492E-4	0.0	0.0	19.577059750891728	0.0
26	5.357743211404492E-4	0.0	0.0	19.912588419505937	0.0
27	5.357743211404492E-4	0.0	0.0	20.292586356774798	0.0
28	5.357743211404492E-4	0.0	0.0	20.688925410838447	0.0
29	5.357743211404492E-4	0.0	0.0	21.08352319835839	0.0
30	5.357743211404492E-4	0.0	0.0	21.5863473987487	0.0
31	5.357743211404492E-4	0.0	0.0	21.952281260087627	0.0
32	5.357743211404492E-4	0.0	0.0	22.337235109827038	0.0
33	5.357743211404492E-4	0.0	0.0	22.729154025741277	0.0
34	5.357743211404492E-4	0.0	0.0	23.080354093248843	0.0
35	5.357743211404492E-4	0.0	0.0	23.459816256196564	0.0
36	5.357743211404492E-4	0.0	0.0	23.80820350851814	0.0
37	5.357743211404492E-4	0.0	0.0	24.166770472941387	0.0
38	5.357743211404492E-4	0.0	0.0	24.57302135194613	0.0
39	5.357743211404492E-4	0.0	0.0	24.956367878722126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTA	30	2.1644755E-6	45.000004	39
GTATACG	30	2.1644755E-6	45.000004	1
ACGTAAG	60	0.0	45.000004	1
TCATACG	45	3.8380676E-10	45.000004	1
TAAGACG	60	0.0	45.000004	1
AAATGCG	30	2.1644755E-6	45.000004	37
AACGTAG	25	3.8894876E-5	45.0	1
GATCGAC	20	7.0316973E-4	45.0	9
TACCACG	35	1.2111559E-7	45.0	1
ACGTTTA	25	3.8894876E-5	45.0	21
CGAAACG	20	7.0316973E-4	45.0	1
TTGTCGC	20	7.0316973E-4	45.0	31
ACCGACG	20	7.0316973E-4	45.0	1
TATATCG	20	7.0316973E-4	45.0	1
CGACACG	35	1.2111559E-7	45.0	1
TACGCCA	25	3.8894876E-5	45.0	14
GTTCGGG	35	1.2111559E-7	45.0	3
CGTAGCG	70	0.0	45.0	1
CGTAACT	35	1.2111559E-7	45.0	27
CGTAACG	35	1.2111559E-7	45.0	1
>>END_MODULE