Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553055_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 759764 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT | 5691 | 0.7490483887101784 | TruSeq Adapter, Index 16 (96% over 25bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1505 | 0.1980878272726794 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT | 1199 | 0.15781216272421436 | TruSeq Adapter, Index 16 (100% over 21bp) |
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC | 1054 | 0.1387272890002685 | TruSeq Adapter, Index 16 (100% over 23bp) |
AATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT | 985 | 0.129645521504046 | TruSeq Adapter, Index 16 (95% over 22bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 831 | 0.10937606941102762 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT | 812 | 0.10687529285409679 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC | 789 | 0.10384803702202262 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCATACG | 55 | 1.8189894E-12 | 45.000004 | 1 |
TAATACG | 25 | 3.889553E-5 | 45.0 | 1 |
CGTATAG | 35 | 1.2111923E-7 | 45.0 | 1 |
AGATACG | 25 | 3.889553E-5 | 45.0 | 1 |
CTATCGC | 20 | 7.031776E-4 | 45.0 | 38 |
ATGGACG | 25 | 3.889553E-5 | 45.0 | 11 |
GACGTAT | 25 | 3.889553E-5 | 45.0 | 42 |
TAGCGAT | 35 | 1.2111923E-7 | 45.0 | 45 |
CAACCCG | 20 | 7.031776E-4 | 45.0 | 1 |
CACGCAA | 20 | 7.031776E-4 | 45.0 | 28 |
CGATTAC | 35 | 1.2111923E-7 | 45.0 | 38 |
CACGACC | 100 | 0.0 | 45.0 | 27 |
TAACGGC | 20 | 7.031776E-4 | 45.0 | 3 |
CGACAAT | 20 | 7.031776E-4 | 45.0 | 32 |
TAGCCCG | 20 | 7.031776E-4 | 45.0 | 1 |
CGTTAGA | 35 | 1.2111923E-7 | 45.0 | 42 |
CCATCGC | 20 | 7.031776E-4 | 45.0 | 30 |
CGTAGCG | 40 | 6.8102963E-9 | 45.0 | 1 |
ACGGATT | 20 | 7.031776E-4 | 45.0 | 19 |
TATTCGA | 25 | 3.889553E-5 | 45.0 | 14 |