FastQCFastQC Report
Sat 18 Jun 2016
SRR3553055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759764
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT56910.7490483887101784TruSeq Adapter, Index 16 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15050.1980878272726794No Hit
AAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT11990.15781216272421436TruSeq Adapter, Index 16 (100% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC10540.1387272890002685TruSeq Adapter, Index 16 (100% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT9850.129645521504046TruSeq Adapter, Index 16 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8310.10937606941102762No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT8120.10687529285409679No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC7890.10384803702202262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACG551.8189894E-1245.0000041
TAATACG253.889553E-545.01
CGTATAG351.2111923E-745.01
AGATACG253.889553E-545.01
CTATCGC207.031776E-445.038
ATGGACG253.889553E-545.011
GACGTAT253.889553E-545.042
TAGCGAT351.2111923E-745.045
CAACCCG207.031776E-445.01
CACGCAA207.031776E-445.028
CGATTAC351.2111923E-745.038
CACGACC1000.045.027
TAACGGC207.031776E-445.03
CGACAAT207.031776E-445.032
TAGCCCG207.031776E-445.01
CGTTAGA351.2111923E-745.042
CCATCGC207.031776E-445.030
CGTAGCG406.8102963E-945.01
ACGGATT207.031776E-445.019
TATTCGA253.889553E-545.014