Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553053_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 379316 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT | 3688 | 0.9722764133334739 | TruSeq Adapter, Index 13 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1794 | 0.47295658501091437 | No Hit |
AAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT | 1062 | 0.27997764396967173 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT | 743 | 0.19587889780552362 | No Hit |
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC | 706 | 0.18612449778021492 | TruSeq Adapter, Index 19 (95% over 21bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 513 | 0.13524343818873974 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 458 | 0.12074365436733489 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT | 429 | 0.11309831380695778 | No Hit |
TGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC | 418 | 0.11019835704267683 | No Hit |
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG | 382 | 0.10070758945048455 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTACCAG | 35 | 1.2090459E-7 | 45.000004 | 1 |
TGAACGG | 30 | 2.1616415E-6 | 45.000004 | 2 |
TCGATCA | 35 | 1.2090459E-7 | 45.000004 | 17 |
CCTCGAT | 35 | 1.2090459E-7 | 45.000004 | 15 |
ATTCCCG | 30 | 2.1616415E-6 | 45.000004 | 1 |
GTTCGGG | 70 | 0.0 | 45.000004 | 3 |
TTAAGTG | 30 | 2.1616415E-6 | 45.000004 | 23 |
AGGTTCG | 30 | 2.1616415E-6 | 45.000004 | 1 |
CGTAGCG | 30 | 2.1616415E-6 | 45.000004 | 1 |
CGCTAGG | 35 | 1.2090459E-7 | 45.000004 | 2 |
ACGGATG | 35 | 1.2090459E-7 | 45.000004 | 37 |
ACGCTAG | 35 | 1.2090459E-7 | 45.000004 | 1 |
CGTACAG | 30 | 2.1616415E-6 | 45.000004 | 1 |
ATATTGG | 35 | 1.2090459E-7 | 45.000004 | 2 |
TATCAGT | 20 | 7.027304E-4 | 45.0 | 10 |
AGCGTAA | 20 | 7.027304E-4 | 45.0 | 41 |
TTGGCGA | 20 | 7.027304E-4 | 45.0 | 25 |
AATCCAT | 20 | 7.027304E-4 | 45.0 | 36 |
TCGTTAA | 20 | 7.027304E-4 | 45.0 | 35 |
TCGCAAT | 25 | 3.8858467E-5 | 45.0 | 29 |