FastQCFastQC Report
Sat 18 Jun 2016
SRR3553053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379316
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT36880.9722764133334739TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.47295658501091437No Hit
AAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT10620.27997764396967173No Hit
AAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT7430.19587889780552362No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC7060.18612449778021492TruSeq Adapter, Index 19 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5130.13524343818873974No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.12074365436733489No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT4290.11309831380695778No Hit
TGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC4180.11019835704267683No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG3820.10070758945048455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAG351.2090459E-745.0000041
TGAACGG302.1616415E-645.0000042
TCGATCA351.2090459E-745.00000417
CCTCGAT351.2090459E-745.00000415
ATTCCCG302.1616415E-645.0000041
GTTCGGG700.045.0000043
TTAAGTG302.1616415E-645.00000423
AGGTTCG302.1616415E-645.0000041
CGTAGCG302.1616415E-645.0000041
CGCTAGG351.2090459E-745.0000042
ACGGATG351.2090459E-745.00000437
ACGCTAG351.2090459E-745.0000041
CGTACAG302.1616415E-645.0000041
ATATTGG351.2090459E-745.0000042
TATCAGT207.027304E-445.010
AGCGTAA207.027304E-445.041
TTGGCGA207.027304E-445.025
AATCCAT207.027304E-445.036
TCGTTAA207.027304E-445.035
TCGCAAT253.8858467E-545.029