##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	379316
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72417456685191	31.0	31.0	34.0	30.0	34.0
2	31.92413976737074	33.0	31.0	34.0	30.0	34.0
3	32.06180598761982	34.0	31.0	34.0	30.0	34.0
4	35.74759303588565	37.0	35.0	37.0	35.0	37.0
5	35.53490757046895	37.0	35.0	37.0	33.0	37.0
6	35.55929357053222	37.0	35.0	37.0	33.0	37.0
7	35.627424100222505	37.0	35.0	37.0	35.0	37.0
8	35.64837233335794	37.0	35.0	37.0	35.0	37.0
9	37.323474359109554	39.0	37.0	39.0	34.0	39.0
10	36.91536344367229	39.0	37.0	39.0	32.0	39.0
11	36.93826255681279	39.0	37.0	39.0	33.0	39.0
12	36.869085406363034	39.0	37.0	39.0	33.0	39.0
13	36.8350082780584	39.0	37.0	39.0	33.0	39.0
14	37.971757057440236	40.0	37.0	41.0	33.0	41.0
15	38.01492423203872	40.0	37.0	41.0	33.0	41.0
16	37.90878845079037	40.0	37.0	41.0	33.0	41.0
17	37.8881249406827	40.0	37.0	41.0	33.0	41.0
18	37.822770460513134	40.0	37.0	41.0	33.0	41.0
19	37.83977211612481	40.0	36.0	41.0	33.0	41.0
20	37.7546610214175	39.0	36.0	41.0	33.0	41.0
21	37.712835735903575	39.0	36.0	41.0	33.0	41.0
22	37.711865568549705	39.0	36.0	41.0	33.0	41.0
23	37.5478967404486	39.0	36.0	41.0	32.0	41.0
24	37.40201573358361	39.0	36.0	41.0	32.0	41.0
25	37.41007497706398	39.0	36.0	41.0	32.0	41.0
26	37.3605384428814	39.0	36.0	41.0	32.0	41.0
27	37.28315705111306	39.0	36.0	41.0	32.0	41.0
28	37.08063988864166	39.0	35.0	41.0	31.0	41.0
29	36.89500047453838	39.0	35.0	41.0	31.0	41.0
30	36.91251884971897	39.0	35.0	41.0	31.0	41.0
31	36.806944605553156	39.0	35.0	41.0	31.0	41.0
32	36.783613135222346	39.0	35.0	40.0	31.0	41.0
33	36.699393118138964	39.0	35.0	40.0	31.0	41.0
34	36.63060614369022	39.0	35.0	40.0	30.0	41.0
35	36.591896993535734	39.0	35.0	40.0	30.0	41.0
36	36.44258349239157	39.0	35.0	40.0	30.0	41.0
37	36.344193232028175	39.0	35.0	40.0	30.0	41.0
38	36.29584040747029	39.0	35.0	40.0	30.0	41.0
39	36.2553886469329	39.0	35.0	40.0	30.0	41.0
40	36.17696854337808	38.0	35.0	40.0	30.0	41.0
41	36.123169072752006	38.0	35.0	40.0	30.0	41.0
42	36.01579685539234	38.0	35.0	40.0	30.0	41.0
43	35.87000811987894	38.0	35.0	40.0	29.0	41.0
44	35.781662255217284	38.0	35.0	40.0	29.0	41.0
45	35.57063767412922	38.0	34.0	40.0	28.0	41.0
46	35.41785213384092	38.0	34.0	40.0	28.0	41.0
47	35.3016614115935	38.0	34.0	40.0	27.0	41.0
48	35.24269474527834	37.0	34.0	40.0	27.0	41.0
49	35.013619251494795	37.0	34.0	40.0	26.0	41.0
50	34.96718567105	37.0	34.0	40.0	26.0	41.0
51	33.69808286494638	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	7.0
12	7.0
13	5.0
14	8.0
15	34.0
16	38.0
17	101.0
18	197.0
19	343.0
20	563.0
21	864.0
22	1193.0
23	1653.0
24	2001.0
25	2328.0
26	2818.0
27	3564.0
28	4644.0
29	5850.0
30	7198.0
31	9307.0
32	12264.0
33	16451.0
34	25042.0
35	31531.0
36	37160.0
37	53390.0
38	77633.0
39	83097.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.392232333990655	31.32296027586498	20.7294182159466	27.555389174197764
2	23.610393445043183	31.035600923768047	20.10513661432684	25.24886901686193
3	21.35053622836896	31.99364118571323	20.48608548018011	26.169737105737696
4	19.401238017905914	33.56225416275612	19.448691855866876	27.58781596347109
5	18.314545128599903	33.93634858534836	17.9491505763005	29.799955709751234
6	20.459985869301587	37.3453795779772	19.13312383342648	23.06151071929473
7	81.36039608136751	6.259952124350146	6.959896234274325	5.4197555600080145
8	82.69780341456728	5.85474907465016	5.626443387571312	5.821004123211254
9	75.20958778432758	8.88836748252117	10.273492286115008	5.628552447036244
10	33.22533191323329	33.57965390334181	14.1657615286463	19.029252654778603
11	25.767434012801992	29.48755127650824	24.931982832255954	19.81303187843381
12	24.84234780499636	24.737949361482247	30.442955214122264	19.97674761939913
13	26.00918495396978	26.038711786478817	25.960940218709467	21.991163040841936
14	19.791941283784496	28.554819728142235	29.16196522160942	22.491273766463845
15	19.403874342237078	29.866918347762816	28.15462569467146	22.574581615328647
16	19.489818515433043	29.184373978424322	26.171846165202627	25.153961340940008
17	19.336648071792382	27.60495207162366	28.42168534941843	24.63671450716553
18	22.82740511868732	25.98150354849255	26.808518491178855	24.38257284164127
19	22.223950479283765	30.930147950521462	25.970430986301658	20.87547058389311
20	23.546330763795886	28.294350884223178	25.692035136930684	22.467283215050248
21	23.13506416813422	29.16591970810617	24.44268103639182	23.256335087367788
22	22.000390176001012	26.281253624945954	25.560218920372456	26.15813727868057
23	21.918400489301796	28.37976779255291	23.74431872106634	25.95751299707895
24	18.682839637663584	29.091311729534215	25.727361882968292	26.498486749833912
25	21.206856552320495	29.868236509928398	23.114764470784255	25.810142466966855
26	24.130276603148825	27.681405477227432	22.896740448596947	25.291577471026795
27	20.796908118824412	27.30968374653323	23.78149089413576	28.111917240506596
28	21.9745541975556	30.395765008594417	25.412584757827243	22.217096036022735
29	21.423035147475982	29.629121893091774	23.728237142646236	25.219605816786007
30	22.223950479283765	29.77807421780257	23.60380263421527	24.394172668698396
31	23.036465638148666	30.354111084162017	19.81540457033186	26.794018707357452
32	21.273819190332073	27.817703445148634	24.879256345632665	26.029221018886627
33	20.04344662497759	28.230815467842113	23.83711733752333	27.888620569656965
34	19.12679665503169	28.22448828944732	25.04297208659798	27.605742968923007
35	19.675415748347024	28.11929894863386	24.272110852165476	27.93317445085364
36	21.36529964462348	29.879572704552405	24.933037361988422	23.822090288835692
37	20.398823144818568	30.043024813084607	22.48942833943203	27.068723702664798
38	20.74128167543684	28.43829419270476	25.817524175094114	25.00289995676428
39	20.95798753545856	24.054350462411286	25.58341857448671	29.404243427643443
40	22.521855128705354	25.46003859578821	23.841335456453194	28.176770819053242
41	20.172890149637766	24.171666895148107	25.603454639403555	30.05198831581056
42	23.15510023305107	25.759525039808494	24.493298463550182	26.592076263590254
43	20.47290385852429	25.5272648662329	26.20901833827205	27.79081293697076
44	22.440129074439255	23.333843022704027	25.176897362621137	29.04913054023558
45	23.678410612787225	23.75249132649295	26.9869976483987	25.582100412321125
46	19.84150418121039	24.740322053380297	28.971886237332463	26.446287528076855
47	19.910575878686902	26.00997585126913	25.636935958409346	28.442512311634626
48	20.667464594164233	24.31798289552774	28.506047728015695	26.508504782292334
49	22.503928123253438	23.696864883105377	27.43754547659471	26.361661517046475
50	20.35796011768552	23.011684189435723	27.994600807769775	28.635754885108987
51	22.003817397631526	22.2421411171688	28.99350409684801	26.76053738835166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2134.0
1	1663.0
2	1192.0
3	802.5
4	413.0
5	406.5
6	400.0
7	404.0
8	408.0
9	477.0
10	546.0
11	614.0
12	682.0
13	641.5
14	601.0
15	649.0
16	697.0
17	920.5
18	1144.0
19	1079.0
20	1014.0
21	944.0
22	874.0
23	1442.0
24	2010.0
25	1731.5
26	1949.0
27	2445.0
28	3324.5
29	4204.0
30	4200.0
31	4196.0
32	4672.5
33	5149.0
34	6736.0
35	8323.0
36	9343.5
37	10364.0
38	10571.0
39	10778.0
40	11974.5
41	13171.0
42	16303.5
43	19436.0
44	22751.0
45	26066.0
46	28790.5
47	31515.0
48	36610.5
49	41706.0
50	38854.5
51	36003.0
52	32527.0
53	29051.0
54	25941.5
55	22832.0
56	21267.5
57	19703.0
58	18969.0
59	18235.0
60	16997.0
61	15759.0
62	14355.5
63	12952.0
64	11484.0
65	10016.0
66	8837.5
67	7659.0
68	6271.0
69	4883.0
70	4281.0
71	3679.0
72	3193.5
73	2708.0
74	2327.5
75	1729.5
76	1512.0
77	1108.0
78	704.0
79	472.5
80	241.0
81	231.0
82	221.0
83	176.5
84	132.0
85	108.0
86	84.0
87	65.5
88	47.0
89	28.5
90	10.0
91	8.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	379316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.492690239326794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15887019388406	20.38826846738899
2	11.253782937152607	6.187935366235158
3	4.024898680147987	3.3196587797395165
4	1.9247753472464424	2.1166900960855646
5	1.149970753209815	1.5807894851141382
6	0.7479801542626914	1.233839201178483
7	0.5315592737876674	1.022979612066009
8	0.37398579507284113	0.8225500494276776
9	0.31215340641887773	0.7723743218842402
>10	3.97521862193273	27.421617890456744
>50	1.3812538356445592	26.20205066995107
>100	0.15956723011056279	6.599043107492177
>500	0.002991885564573052	0.5379475892014147
>1k	0.002991885564573052	1.7942553637788268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	3688	0.9722764133334739	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1794	0.47295658501091437	No Hit
AAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	1062	0.27997764396967173	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	743	0.19587889780552362	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	706	0.18612449778021492	TruSeq Adapter, Index 19 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	513	0.13524343818873974	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	458	0.12074365436733489	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	429	0.11309831380695778	No Hit
TGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	418	0.11019835704267683	No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	382	0.10070758945048455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0977654514969049	0.0
2	0.0	0.0	0.0	1.5003321768657267	0.0
3	0.0	0.0	0.0	1.938752913138386	0.0
4	0.0	0.0	0.0	3.9623954697402692	0.0
5	0.0	0.0	0.0	4.294572335466999	0.0
6	0.0	0.0	0.0	5.913275474802012	0.0
7	0.0	0.0	0.0	7.114121207647449	0.0
8	0.0	0.0	0.0	8.14466038869966	0.0
9	0.0	0.0	0.0	9.368442143226229	0.0
10	0.0	0.0	0.0	11.450611099979964	0.0
11	0.0	0.0	0.0	13.055868985226038	0.0
12	0.0	0.0	0.0	13.99413681468749	0.0
13	0.0	0.0	0.0	14.410412426578368	0.0
14	0.0	0.0	0.0	14.823788081704963	0.0
15	0.0	0.0	0.0	15.162028493393372	0.0
16	0.0	0.0	0.0	15.848790981661727	0.0
17	0.0	0.0	0.0	16.69636925413112	0.0
18	0.0	0.0	0.0	17.955214122262177	0.0
19	0.0	0.0	0.0	18.40286199369391	0.0
20	0.0	0.0	0.0	18.913781649073595	0.0
21	0.0	0.0	0.0	19.415474169294203	0.0
22	0.0	0.0	0.0	19.91110314355313	0.0
23	0.0	0.0	0.0	20.4634130909321	0.0
24	2.636324331164517E-4	0.0	0.0	20.872834259561948	0.0
25	2.636324331164517E-4	0.0	0.0	21.227419882103575	0.0
26	2.636324331164517E-4	0.0	0.0	21.55274230456928	0.0
27	2.636324331164517E-4	0.0	0.0	21.947136424511488	0.0
28	2.636324331164517E-4	0.0	0.0	22.294340338925856	0.0
29	2.636324331164517E-4	0.0	0.0	22.667116599352518	0.0
30	2.636324331164517E-4	0.0	0.0	23.2036086007445	0.0
31	2.636324331164517E-4	0.0	0.0	23.535258201604993	0.0
32	2.636324331164517E-4	0.0	0.0	23.900652753904396	0.0
33	2.636324331164517E-4	0.0	0.0	24.255238376446023	0.0
34	2.636324331164517E-4	0.0	0.0	24.58688797730652	0.0
35	2.636324331164517E-4	0.0	0.0	24.982600259414316	0.0
36	2.636324331164517E-4	0.0	0.0	25.280241276402787	0.0
37	2.636324331164517E-4	0.0	0.0	25.59159117991332	0.0
38	2.636324331164517E-4	0.0	0.0	25.921131721308882	0.0
39	2.636324331164517E-4	0.0	0.0	26.273871916818695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCAG	35	1.2090459E-7	45.000004	1
TGAACGG	30	2.1616415E-6	45.000004	2
TCGATCA	35	1.2090459E-7	45.000004	17
CCTCGAT	35	1.2090459E-7	45.000004	15
ATTCCCG	30	2.1616415E-6	45.000004	1
GTTCGGG	70	0.0	45.000004	3
TTAAGTG	30	2.1616415E-6	45.000004	23
AGGTTCG	30	2.1616415E-6	45.000004	1
CGTAGCG	30	2.1616415E-6	45.000004	1
CGCTAGG	35	1.2090459E-7	45.000004	2
ACGGATG	35	1.2090459E-7	45.000004	37
ACGCTAG	35	1.2090459E-7	45.000004	1
CGTACAG	30	2.1616415E-6	45.000004	1
ATATTGG	35	1.2090459E-7	45.000004	2
TATCAGT	20	7.027304E-4	45.0	10
AGCGTAA	20	7.027304E-4	45.0	41
TTGGCGA	20	7.027304E-4	45.0	25
AATCCAT	20	7.027304E-4	45.0	36
TCGTTAA	20	7.027304E-4	45.0	35
TCGCAAT	25	3.8858467E-5	45.0	29
>>END_MODULE