SRR3553050_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	710226
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	7893	1.1113363915148136	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT	7074	0.9960209848696049	No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	6613	0.9311120685528269	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3245	0.4568968187590992	TruSeq Adapter, Index 15 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	1711	0.2409092317093432	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	1434	0.2019075618183508	No Hit
AACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	1286	0.18106912447587106	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTATC	1262	0.1776899184203338	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTAT	1226	0.17262110933702793	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1175	0.16544029646901126	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.13784344701545703	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	747	0.10517778847859696	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.0314633E-4	45.000004	30
CGAACAG	40	6.8084773E-9	45.000004	1
GCGAAGT	20	7.0314633E-4	45.000004	32
CTATACG	20	7.0314633E-4	45.000004	1
CGGTTAG	30	2.1643245E-6	45.000004	1
CGACAAA	20	7.0314633E-4	45.000004	43
AATTGCG	30	2.1643245E-6	45.000004	1
TACGAAT	60	0.0	45.000004	11
CCATACG	30	2.1643245E-6	45.000004	1
CGAATAT	60	0.0	45.000004	13
ACGCTAG	20	7.0314633E-4	45.000004	1
TGGATCG	20	7.0314633E-4	45.000004	37
TAGGCGT	20	7.0314633E-4	45.000004	29
ACTCACG	20	7.0314633E-4	45.000004	1
CGCCCGA	20	7.0314633E-4	45.000004	37
CTAGCAG	100	0.0	42.749996	1
CTACGAA	65	0.0	41.53846	10
TTTGCGC	60	3.6379788E-12	41.250004	13
TACGCGG	105	0.0	40.714287	2
TAAACGG	50	1.0804797E-9	40.499996	2