##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	710226
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.888152503569287	33.0	31.0	34.0	30.0	34.0
2	32.08894070338174	33.0	31.0	34.0	30.0	34.0
3	32.22892853823994	34.0	31.0	34.0	30.0	34.0
4	35.86324775493998	37.0	35.0	37.0	35.0	37.0
5	35.624657503386246	37.0	35.0	37.0	33.0	37.0
6	34.9667232683681	37.0	35.0	37.0	33.0	37.0
7	35.29563406577625	37.0	35.0	37.0	33.0	37.0
8	35.71874586399259	37.0	35.0	37.0	33.0	37.0
9	37.43107827649227	39.0	37.0	39.0	35.0	39.0
10	37.043051085147546	39.0	37.0	39.0	33.0	39.0
11	37.0213382782382	39.0	37.0	39.0	33.0	39.0
12	36.82404192468313	39.0	37.0	39.0	33.0	39.0
13	36.84357091967909	39.0	37.0	39.0	33.0	39.0
14	38.021365030286134	40.0	37.0	41.0	33.0	41.0
15	38.0648371081881	40.0	37.0	41.0	33.0	41.0
16	37.92344267880928	40.0	37.0	41.0	33.0	41.0
17	37.93072627586149	40.0	37.0	41.0	33.0	41.0
18	37.865348494704506	40.0	37.0	41.0	33.0	41.0
19	37.875980321756735	40.0	37.0	41.0	33.0	41.0
20	37.86126247138235	40.0	37.0	41.0	33.0	41.0
21	37.87549456088625	40.0	37.0	41.0	33.0	41.0
22	37.833474133585646	40.0	37.0	41.0	33.0	41.0
23	37.74116689617108	40.0	36.0	41.0	33.0	41.0
24	37.60937222799503	39.0	36.0	41.0	33.0	41.0
25	37.63579480334429	40.0	36.0	41.0	33.0	41.0
26	37.58014913562725	40.0	36.0	41.0	33.0	41.0
27	37.495035383103406	39.0	36.0	41.0	32.0	41.0
28	37.43491508336783	39.0	36.0	41.0	32.0	41.0
29	37.20921368691093	39.0	36.0	41.0	32.0	41.0
30	37.34079293070093	39.0	36.0	41.0	32.0	41.0
31	37.264045529169586	39.0	36.0	41.0	32.0	41.0
32	37.22495797112468	39.0	36.0	41.0	32.0	41.0
33	37.04740744495414	39.0	35.0	41.0	31.0	41.0
34	36.948706468081994	39.0	35.0	41.0	31.0	41.0
35	36.92475071315328	39.0	35.0	41.0	31.0	41.0
36	36.885180210242936	39.0	35.0	41.0	31.0	41.0
37	36.798780951415466	39.0	35.0	41.0	31.0	41.0
38	36.64347123310045	39.0	35.0	40.0	31.0	41.0
39	36.52236048806999	39.0	35.0	40.0	30.0	41.0
40	36.42476056917094	39.0	35.0	40.0	30.0	41.0
41	36.39360147333384	39.0	35.0	40.0	30.0	41.0
42	36.39713555966692	39.0	35.0	40.0	30.0	41.0
43	36.37827677387198	38.0	35.0	40.0	30.0	41.0
44	36.30946346655853	38.0	35.0	40.0	30.0	41.0
45	36.075971592141094	38.0	35.0	40.0	30.0	41.0
46	35.88828485580646	38.0	35.0	40.0	30.0	41.0
47	35.808522920873074	38.0	35.0	40.0	29.0	41.0
48	35.71012888855097	38.0	35.0	40.0	29.0	41.0
49	35.590227617687894	38.0	34.0	40.0	29.0	41.0
50	35.51245800632475	37.0	34.0	40.0	29.0	41.0
51	34.18811617710419	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	2.0
11	5.0
12	7.0
13	9.0
14	25.0
15	29.0
16	61.0
17	111.0
18	233.0
19	421.0
20	823.0
21	1384.0
22	1849.0
23	2492.0
24	3082.0
25	3663.0
26	4412.0
27	5608.0
28	7093.0
29	9149.0
30	12435.0
31	16127.0
32	21933.0
33	30203.0
34	47303.0
35	57278.0
36	67507.0
37	96907.0
38	143876.0
39	176137.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.193572186881358	34.76611670088113	18.980014812186543	26.06029630005097
2	23.120527831985875	33.17887545654482	19.192482392928447	24.50811431854086
3	20.666942635161202	28.38406366424209	24.027985458149942	26.92100824244677
4	24.725932308870696	29.182541895115076	18.342189669203886	27.749336126810338
5	18.69010709267191	34.78864474125138	17.61298516246941	28.908263003607303
6	20.312970800843676	36.233255329993554	21.6478979930332	21.805875876129573
7	81.37480182364487	8.138254583752214	6.012311574062341	4.474632018540578
8	82.82476282197497	3.980141532413626	4.588961823419588	8.60613382219181
9	78.25114822605762	7.153779219572362	8.508981648095114	6.086090906274904
10	38.556881893932356	29.628315493941365	13.896844103144632	17.91795850898165
11	28.382655661718946	27.088560542700492	22.66686941903	21.861914376550562
12	27.615294286607362	22.864975374035872	29.723496464505665	19.796233874851104
13	25.67013880088873	25.00964481728351	26.103240377006752	23.216976004821
14	21.632269165026344	25.32250297792534	29.48554403809492	23.559683818953403
15	20.180196162911525	27.8160754464072	26.872432155398425	25.131296235282854
16	22.932981895903556	26.080993937141134	27.99925657466778	22.986767592287524
17	22.238695851742964	25.994824182724933	26.931286660865695	24.835193304666404
18	23.00014361625736	25.765882972462283	26.687561424110072	24.54641198717028
19	23.571229439643158	29.036954434222345	26.237000616705103	21.15481550942939
20	25.66802679710402	28.215807362726792	24.295083536789697	21.821082303379487
21	24.693407450586154	25.901192014936093	25.936110477509978	23.469290056967782
22	23.052802910622816	26.285295103248824	25.441056790373768	25.220845195754592
23	23.16544311247406	26.59660446111519	23.99869900566862	26.23925342074213
24	22.236724648210572	29.36755342665574	24.77394519490979	23.6217767302239
25	24.30451715369474	25.933012871959065	24.292267531743416	25.47020244260278
26	25.314054962786493	26.554364385420982	23.343555431651332	24.788025220141193
27	22.071143551489243	25.18958753974087	25.69675004857609	27.0425188601938
28	23.169526319791164	25.677742014513687	26.904816213430653	24.24791545226449
29	21.930061698670563	30.333865558287084	23.90112443081498	23.834948312227375
30	24.715513090199455	24.602168887086645	25.07736973864657	25.604948284067326
31	23.77327780171382	28.575974408146138	21.86810958765238	25.782638202487657
32	22.976911574625543	27.335664985511656	23.211907195737695	26.475516244125107
33	25.428807168422445	25.520468132678893	24.413214948481187	24.637509750417472
34	20.91770788453253	27.560241387952566	26.035093054886755	25.486957672628147
35	22.33190561877487	27.26061845102826	25.850363123850716	24.557112806346147
36	22.642792575884297	29.119181781573754	25.342496613753934	22.89552902878802
37	22.377665700776937	29.581992210930043	22.500865921551732	25.53947616674129
38	24.70551627228516	26.99563237617322	24.65764418649838	23.64120716504324
39	22.00834663895718	26.2139093753256	26.984227555735778	24.793516429981445
40	24.00292301323804	26.827657675162552	25.679290817289147	23.490128494310262
41	21.278156530456503	24.857580544784337	29.778690163412776	24.085572761346388
42	22.808655273110247	27.409725918228844	25.10200978280153	24.679609025859374
43	22.217012612886602	24.705797872789788	27.556580581392403	25.52060893293121
44	23.436765198683236	22.215604610363464	26.27515748508221	28.07247270587109
45	25.802772638568566	24.912351842934502	25.5406025687598	23.744272949737123
46	21.027109680580548	25.20662437027087	30.112527561649387	23.65373838749919
47	22.75444717596934	26.653628563302384	25.48906967641286	25.102854584315416
48	21.733504546440148	23.940689301715228	29.477377623460697	24.848428528383923
49	22.63349975923157	25.836564699123944	26.86257613773644	24.66735940390805
50	22.321345599851313	23.73920414065382	26.787388803000734	27.15206145649413
51	21.870643992194033	23.29864015116315	29.338830175183674	25.49188568145914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1286.0
1	1063.5
2	841.0
3	592.0
4	343.0
5	351.0
6	359.0
7	381.0
8	403.0
9	470.5
10	538.0
11	567.5
12	597.0
13	708.0
14	819.0
15	828.5
16	838.0
17	901.5
18	965.0
19	1066.0
20	1167.0
21	1234.0
22	1301.0
23	2197.0
24	3093.0
25	3384.0
26	4300.0
27	4925.0
28	6179.5
29	7434.0
30	8551.5
31	9669.0
32	11262.0
33	12855.0
34	14902.5
35	16950.0
36	18333.0
37	19716.0
38	21398.0
39	23080.0
40	25105.5
41	27131.0
42	38826.0
43	50521.0
44	53833.0
45	57145.0
46	59804.0
47	62463.0
48	59546.0
49	56629.0
50	54245.5
51	51862.0
52	47333.0
53	42804.0
54	40475.5
55	38147.0
56	36741.0
57	35335.0
58	35499.5
59	35664.0
60	34676.0
61	33688.0
62	30343.5
63	26999.0
64	24942.0
65	22885.0
66	19997.0
67	17109.0
68	14607.0
69	12105.0
70	10579.5
71	9054.0
72	8004.5
73	6955.0
74	5646.5
75	3868.5
76	3399.0
77	2826.5
78	2254.0
79	1830.0
80	1406.0
81	1096.0
82	786.0
83	569.5
84	353.0
85	277.0
86	201.0
87	134.0
88	67.0
89	52.0
90	37.0
91	30.0
92	23.0
93	16.0
94	9.0
95	6.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	710226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.45692782691178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94674627405091	19.333708385872953
2	8.824660808105403	4.4929750657783245
3	3.092077908725376	2.3614441247283064
4	1.5636525444693972	1.592231598836976
5	0.9629491962255382	1.2256864094648119
6	0.6770279362519946	1.0341030785982035
7	0.531474903751138	0.9470802786625324
8	0.4250436907894196	0.865624524776837
9	0.3512505763059901	0.8047584513164485
>10	6.475011817784501	42.07150132305085
>50	1.078399261738883	17.22004965262873
>100	0.06380058459502283	2.89302928383242
>500	0.0022584277732751444	0.42630787240893075
>1k	0.003952248603231502	1.6297724090508652
>5k	0.0016938208299563582	3.1017275409928273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	7893	1.1113363915148136	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGT	7074	0.9960209848696049	No Hit
AATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	6613	0.9311120685528269	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3245	0.4568968187590992	TruSeq Adapter, Index 15 (95% over 24bp)
AATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	1711	0.2409092317093432	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	1434	0.2019075618183508	No Hit
AACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	1286	0.18106912447587106	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTATC	1262	0.1776899184203338	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTAT	1226	0.17262110933702793	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1175	0.16544029646901126	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	979	0.13784344701545703	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	747	0.10517778847859696	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4080025231405215E-4	0.0	0.0	0.5175817275064557	0.0
2	1.4080025231405215E-4	0.0	0.0	0.7192076888201784	0.0
3	1.4080025231405215E-4	0.0	0.0	1.1249940159892766	0.0
4	1.4080025231405215E-4	0.0	0.0	2.9071028095282347	0.0
5	1.4080025231405215E-4	0.0	0.0	3.1257656013719575	0.0
6	1.4080025231405215E-4	0.0	0.0	4.198241123248093	0.0
7	1.4080025231405215E-4	0.0	0.0	5.002773764970587	0.0
8	1.4080025231405215E-4	0.0	0.0	5.589910817120185	0.0
9	1.4080025231405215E-4	0.0	0.0	7.303309087529885	0.0
10	1.4080025231405215E-4	0.0	0.0	8.381135018993954	0.0
11	1.4080025231405215E-4	0.0	0.0	10.459628343653991	0.0
12	1.4080025231405215E-4	0.0	0.0	10.973549264600281	0.0
13	1.4080025231405215E-4	0.0	0.0	11.269933795721363	0.0
14	1.4080025231405215E-4	0.0	0.0	11.678676928189056	0.0
15	1.4080025231405215E-4	0.0	0.0	11.924654968981704	0.0
16	1.4080025231405215E-4	1.4080025231405215E-4	0.0	12.297071636352372	0.0
17	1.4080025231405215E-4	1.4080025231405215E-4	0.0	12.717501189762132	0.0
18	1.4080025231405215E-4	1.4080025231405215E-4	0.0	13.495844984554212	0.0
19	1.4080025231405215E-4	1.4080025231405215E-4	0.0	13.77997989372397	0.0
20	1.4080025231405215E-4	1.4080025231405215E-4	0.0	14.102271671270834	0.0
21	1.4080025231405215E-4	1.4080025231405215E-4	0.0	14.457088307102246	0.0
22	1.4080025231405215E-4	1.4080025231405215E-4	0.0	14.789236102311095	0.0
23	1.4080025231405215E-4	1.4080025231405215E-4	0.0	15.143771137637879	0.0
24	1.4080025231405215E-4	1.4080025231405215E-4	0.0	15.409742814259124	0.0
25	1.4080025231405215E-4	1.4080025231405215E-4	0.0	15.654312852528632	0.0
26	1.4080025231405215E-4	1.4080025231405215E-4	0.0	15.876214050175578	0.0
27	1.4080025231405215E-4	1.4080025231405215E-4	0.0	16.117827283146493	0.0
28	1.4080025231405215E-4	1.4080025231405215E-4	0.0	16.3614117196498	0.0
29	1.4080025231405215E-4	1.4080025231405215E-4	0.0	16.63160740384047	0.0
30	1.4080025231405215E-4	1.4080025231405215E-4	0.0	16.98008802831775	0.0
31	1.4080025231405215E-4	1.4080025231405215E-4	0.0	17.2150836494299	0.0
32	1.4080025231405215E-4	1.4080025231405215E-4	0.0	17.46936890510908	0.0
33	1.4080025231405215E-4	1.4080025231405215E-4	0.0	17.745619000149247	0.0
34	1.4080025231405215E-4	1.4080025231405215E-4	0.0	17.986528231858593	0.0
35	1.4080025231405215E-4	1.4080025231405215E-4	0.0	18.26235592614182	0.0
36	1.4080025231405215E-4	1.4080025231405215E-4	0.0	18.510305170466864	0.0
37	1.4080025231405215E-4	1.4080025231405215E-4	0.0	18.746567993849844	0.0
38	1.4080025231405215E-4	1.4080025231405215E-4	0.0	18.99902284624894	0.0
39	1.4080025231405215E-4	1.4080025231405215E-4	0.0	19.25471610445126	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.0314633E-4	45.000004	30
CGAACAG	40	6.8084773E-9	45.000004	1
GCGAAGT	20	7.0314633E-4	45.000004	32
CTATACG	20	7.0314633E-4	45.000004	1
CGGTTAG	30	2.1643245E-6	45.000004	1
CGACAAA	20	7.0314633E-4	45.000004	43
AATTGCG	30	2.1643245E-6	45.000004	1
TACGAAT	60	0.0	45.000004	11
CCATACG	30	2.1643245E-6	45.000004	1
CGAATAT	60	0.0	45.000004	13
ACGCTAG	20	7.0314633E-4	45.000004	1
TGGATCG	20	7.0314633E-4	45.000004	37
TAGGCGT	20	7.0314633E-4	45.000004	29
ACTCACG	20	7.0314633E-4	45.000004	1
CGCCCGA	20	7.0314633E-4	45.000004	37
CTAGCAG	100	0.0	42.749996	1
CTACGAA	65	0.0	41.53846	10
TTTGCGC	60	3.6379788E-12	41.250004	13
TACGCGG	105	0.0	40.714287	2
TAAACGG	50	1.0804797E-9	40.499996	2
>>END_MODULE