FastQCFastQC Report
Sat 18 Jun 2016
SRR3553048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898370
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT37030.4121909680866458TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT32410.36076449569776375No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT30480.33928114251366365No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC29200.3250331155314625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9800.10908645658247716No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9070.10096062869419059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG302.1649703E-645.0000041
TCGATAG253.8901224E-545.0000041
ATGTACG253.8901224E-545.0000041
CGTAACG302.1649703E-645.0000041
TCGAACG253.8901224E-545.0000041
CTATCGC207.0324645E-445.018
TTGTCGA207.0324645E-445.029
TCGGTAT351.2115379E-745.020
TACGTCG207.0324645E-445.024
TTAACGG1450.043.448282
TACGAAT1050.042.85714311
TACGCGG1650.042.2727282
AACGGGA10550.041.8009454
ATTAGCG603.6379788E-1241.2500041
CGACACG556.002665E-1140.9090921
CGGGATT2950.040.4237256
TGCAACG3800.040.2631571
ACGGGTA1350.040.05
ACTACGG1650.039.5454562
CTAGCGG2050.039.5121962
Produced by FastQC (version 0.11.5)