##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	898370
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865904916682435	33.0	31.0	34.0	30.0	34.0
2	32.068996070661306	34.0	31.0	34.0	30.0	34.0
3	32.219934993376896	34.0	31.0	34.0	30.0	34.0
4	35.86556318666028	37.0	35.0	37.0	35.0	37.0
5	35.637304228769885	37.0	35.0	37.0	33.0	37.0
6	34.97144383717177	37.0	35.0	37.0	33.0	37.0
7	35.39414049890357	37.0	35.0	37.0	33.0	37.0
8	35.78921045894231	37.0	35.0	37.0	35.0	37.0
9	37.4602947560582	39.0	37.0	39.0	35.0	39.0
10	37.03548760532965	39.0	37.0	39.0	33.0	39.0
11	37.01832207219742	39.0	37.0	39.0	33.0	39.0
12	36.89376426194107	39.0	37.0	39.0	33.0	39.0
13	36.87330943820475	39.0	37.0	39.0	33.0	39.0
14	38.07436468270312	40.0	37.0	41.0	33.0	41.0
15	38.11005265091221	40.0	37.0	41.0	33.0	41.0
16	37.980075024767075	40.0	37.0	41.0	33.0	41.0
17	37.974886739316766	40.0	37.0	41.0	33.0	41.0
18	37.965130180215276	40.0	37.0	41.0	33.0	41.0
19	38.006564110555786	40.0	37.0	41.0	33.0	41.0
20	37.93165510869686	40.0	37.0	41.0	33.0	41.0
21	37.91666907844207	40.0	37.0	41.0	33.0	41.0
22	37.90457049990538	40.0	37.0	41.0	33.0	41.0
23	37.804391286440996	40.0	37.0	41.0	33.0	41.0
24	37.65817090953616	40.0	36.0	41.0	33.0	41.0
25	37.70678784910449	40.0	36.0	41.0	33.0	41.0
26	37.6556674866703	40.0	36.0	41.0	33.0	41.0
27	37.56321337533533	40.0	36.0	41.0	33.0	41.0
28	37.501813284058905	40.0	36.0	41.0	32.0	41.0
29	37.27703062212674	39.0	36.0	41.0	32.0	41.0
30	37.379806761134056	39.0	36.0	41.0	32.0	41.0
31	37.292508654563264	39.0	36.0	41.0	32.0	41.0
32	37.2618709440431	39.0	36.0	41.0	32.0	41.0
33	37.054725781136945	39.0	35.0	41.0	31.0	41.0
34	36.95301713102619	39.0	35.0	41.0	31.0	41.0
35	36.931971236795526	39.0	35.0	41.0	31.0	41.0
36	36.860055433729976	39.0	35.0	41.0	31.0	41.0
37	36.83299308748066	39.0	35.0	41.0	31.0	41.0
38	36.63876687778978	39.0	35.0	40.0	31.0	41.0
39	36.60865456326458	39.0	35.0	40.0	30.0	41.0
40	36.54138940525618	39.0	35.0	40.0	30.0	41.0
41	36.470622349366074	39.0	35.0	40.0	30.0	41.0
42	36.43654396295513	39.0	35.0	40.0	30.0	41.0
43	36.355467123790866	38.0	35.0	40.0	30.0	41.0
44	36.28115809744315	38.0	35.0	40.0	30.0	41.0
45	36.0814586417623	38.0	35.0	40.0	30.0	41.0
46	35.93191446731302	38.0	35.0	40.0	29.0	41.0
47	35.79955140977548	38.0	34.0	40.0	29.0	41.0
48	35.6803989447555	38.0	34.0	40.0	29.0	41.0
49	35.59461357792446	38.0	34.0	40.0	28.0	41.0
50	35.50151830537529	37.0	34.0	40.0	28.0	41.0
51	34.288984494139385	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	13.0
11	8.0
12	7.0
13	17.0
14	31.0
15	53.0
16	77.0
17	180.0
18	300.0
19	523.0
20	1041.0
21	1490.0
22	2178.0
23	2800.0
24	3570.0
25	4541.0
26	5751.0
27	7218.0
28	9402.0
29	12027.0
30	15449.0
31	20417.0
32	27022.0
33	37209.0
34	56966.0
35	70583.0
36	85363.0
37	124265.0
38	187107.0
39	222707.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.65254850451373	31.287554125805627	20.399946569898816	26.65995079978183
2	25.153555884546456	29.35750303327137	19.312087447265604	26.17685363491657
3	21.51507730667765	29.3695248060376	20.985451428698642	28.129946458586108
4	21.487471754399635	31.167447710854102	18.827654529870767	28.517426004875496
5	18.906463929116065	33.77650633925888	17.638723465832566	29.678306265792493
6	20.4012823224284	36.62945111702305	20.327593307879827	22.641673252668724
7	85.29748321960885	5.317296882130971	5.1771541792357265	4.2080657190244555
8	86.52025334772978	3.887262486503334	4.2596035041241365	5.332880661642752
9	79.78227233767824	6.889588922159022	7.9241292563197785	5.40400948384296
10	36.913855093113085	31.102775025880202	13.74789897258368	18.235470908423032
11	28.102786157151282	29.120740897403074	22.955797722541938	19.820675222903702
12	26.727517615236486	24.652203435110255	28.6319667842871	19.98831216536616
13	25.690083150594965	25.58522657702283	26.647149838040008	22.077540434342197
14	22.861070605652458	26.603849193539407	28.809176619878222	21.725903580929906
15	21.429923082916837	27.47587296993444	27.750704052895802	23.343499894252925
16	23.60675445529125	27.621915246502	26.69200886049178	22.07932143771497
17	22.75209546178078	27.079822344913566	26.762469806427198	23.405612386878456
18	24.781214866925655	26.082571768870288	26.03927112436969	23.096942239834366
19	24.258156438883756	28.23023921101551	25.598361476897047	21.913242873203693
20	25.853824148179484	27.339960150049535	24.9427296102942	21.863486091476787
21	25.26976635462003	27.2564756169507	25.213998686510013	22.259759341919253
22	24.348987610895286	25.896679541836882	25.582777697385268	24.171555149882565
23	23.797655754310583	27.047430346071216	24.979462804857686	24.17545109476051
24	21.758295579772255	27.390162182619633	26.426416732526686	24.425125505081425
25	24.506383783964292	27.10186226165166	24.332513329697118	24.059240624686932
26	25.092667831739707	26.771486136001872	24.13604639513786	23.999799637120564
27	23.027037857452942	25.779801195498514	25.738949430635483	25.45421151641306
28	24.32360831283324	27.445039349043267	25.4943954050113	22.736956933112193
29	23.475516769259883	29.153912085221013	24.62404131927825	22.74652982624086
30	25.052372630430668	25.348241815732937	26.158153099502435	23.44123245433396
31	25.13240646949475	28.065830337166204	23.397152620857774	23.40461057248127
32	24.57606554092412	26.595834678361925	25.322417266827703	23.50568251388626
33	25.300822600932797	26.189543283947593	24.650645057159075	23.858989057960528
34	23.224617919120185	27.975555728708663	26.289613410955397	22.510212941215755
35	25.274664113895167	27.220298985941206	26.1391186259559	21.365918274207733
36	23.380010463394814	29.77180894286319	25.527121341986042	21.32105925175596
37	24.236116522145664	28.596235404120797	24.501819962821557	22.665828110911985
38	24.88106236851186	28.483141689949576	25.315515878758195	21.32028006278037
39	24.05957456281933	26.0389371862373	25.574429244075382	24.327059006867994
40	27.20204370137026	24.911784676692232	25.587341518528	22.298830103409507
41	23.416187094404307	24.905885103019916	28.13807228647439	23.539855516101383
42	26.255996972294266	25.380633814575287	24.976012110822936	23.38735710230751
43	23.41117802241838	25.703551988601582	26.614869151908454	24.270400837071584
44	24.82017431570511	24.339192092345023	25.903246991773994	24.937386600175873
45	26.110066008437506	24.70051315159678	26.023353406725512	23.1660674332402
46	22.666607299887573	24.70273940581275	28.63408172579227	23.996571568507406
47	23.832607945501298	26.379331455858946	26.150472522457342	23.63758807618242
48	23.4668343778176	25.567527856005878	28.029653706156704	22.935984060019816
49	24.316929550185336	25.202199539165377	26.697240557899306	23.78363035274998
50	22.672172935427497	25.608602246290502	26.59238398432717	25.126840833954827
51	23.470507697273952	24.11400647839977	28.05514431692955	24.36034150739673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1282.0
1	1054.0
2	826.0
3	615.5
4	405.0
5	405.5
6	406.0
7	473.0
8	540.0
9	610.0
10	680.0
11	739.5
12	799.0
13	852.5
14	906.0
15	955.0
16	1004.0
17	1058.5
18	1113.0
19	1287.0
20	1461.0
21	1780.0
22	2099.0
23	2996.5
24	3894.0
25	4010.0
26	5345.5
27	6565.0
28	8599.0
29	10633.0
30	11632.5
31	12632.0
32	14859.0
33	17086.0
34	19493.0
35	21900.0
36	23878.0
37	25856.0
38	28276.5
39	30697.0
40	34678.5
41	38660.0
42	46909.0
43	55158.0
44	59992.5
45	64827.0
46	70587.5
47	76348.0
48	74876.0
49	73404.0
50	70719.5
51	68035.0
52	63083.0
53	58131.0
54	55404.0
55	52677.0
56	50048.5
57	47420.0
58	46974.5
59	46529.0
60	43658.0
61	40787.0
62	37918.5
63	35050.0
64	30425.0
65	25800.0
66	22812.0
67	19824.0
68	17088.0
69	14352.0
70	12945.0
71	11538.0
72	10277.5
73	9017.0
74	7498.0
75	5025.0
76	4071.0
77	3391.0
78	2711.0
79	2181.5
80	1652.0
81	1211.0
82	770.0
83	548.0
84	326.0
85	287.0
86	248.0
87	150.5
88	53.0
89	44.5
90	36.0
91	40.5
92	45.0
93	27.0
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	898370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.870015864453748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.57815408932426	19.552080452969488
2	9.398708404185689	4.862894710433172
3	3.209824762978972	2.491146526211475
4	1.6484338972558399	1.705800442940476
5	0.9576718548965455	1.238749303955724
6	0.6887604679698284	1.069094653991283
7	0.535849539689559	0.9703705264910392
8	0.43953797469584827	0.9096683502729173
9	0.3804783497476476	0.8858682849647544
>10	5.66440346599452	36.302878398335196
>50	1.3888436290544477	23.701367594315357
>100	0.10362538615588918	4.084229586864254
>500	0.003951815573741536	0.7591407684331473
>1k	0.0017563624772184607	1.466710399821756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3703	0.4121909680866458	TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	3241	0.36076449569776375	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT	3048	0.33928114251366365	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	2920	0.3250331155314625	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	980	0.10908645658247716	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	907	0.10096062869419059	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.339381323953382E-4	0.0	0.0	0.4616138116811559	0.0
2	3.339381323953382E-4	0.0	0.0	0.6697685808742501	0.0
3	3.339381323953382E-4	0.0	0.0	0.9768803499671628	0.0
4	3.339381323953382E-4	0.0	0.0	2.2212451439829914	0.0
5	3.339381323953382E-4	0.0	0.0	2.4241682157685585	0.0
6	3.339381323953382E-4	0.0	0.0	3.391920923450249	0.0
7	3.339381323953382E-4	0.0	0.0	4.076494094860692	0.0
8	3.339381323953382E-4	0.0	0.0	4.693166512684083	0.0
9	3.339381323953382E-4	0.0	0.0	5.728040784977236	0.0
10	3.339381323953382E-4	0.0	0.0	6.889143671315828	0.0
11	3.339381323953382E-4	0.0	0.0	8.137849661052796	0.0
12	3.339381323953382E-4	0.0	0.0	8.627625588565959	0.0
13	3.339381323953382E-4	0.0	0.0	8.858599463472734	0.0
14	3.339381323953382E-4	0.0	0.0	9.125527343967407	0.0
15	3.339381323953382E-4	0.0	0.0	9.343477631710766	0.0
16	3.339381323953382E-4	0.0	0.0	9.75711566503779	0.0
17	3.339381323953382E-4	0.0	0.0	10.23854313924107	0.0
18	3.339381323953382E-4	0.0	0.0	10.921780558121933	0.0
19	3.339381323953382E-4	0.0	0.0	11.21353117312466	0.0
20	3.339381323953382E-4	0.0	0.0	11.562830459610183	0.0
21	3.339381323953382E-4	0.0	0.0	11.93550541536338	0.0
22	3.339381323953382E-4	0.0	0.0	12.333448356467825	0.0
23	3.339381323953382E-4	0.0	0.0	12.724601222213565	0.0
24	3.339381323953382E-4	0.0	0.0	13.016797088059485	0.0
25	3.339381323953382E-4	0.0	0.0	13.292741298128833	0.0
26	3.339381323953382E-4	0.0	0.0	13.532842815321082	0.0
27	3.339381323953382E-4	0.0	0.0	13.81513184990594	0.0
28	3.339381323953382E-4	0.0	0.0	14.10320914545232	0.0
29	4.452508431937843E-4	0.0	0.0	14.363680888720683	0.0
30	4.452508431937843E-4	0.0	0.0	14.839097476540847	0.0
31	4.452508431937843E-4	0.0	0.0	15.116934002693768	0.0
32	4.452508431937843E-4	0.0	0.0	15.421485579438317	0.0
33	4.452508431937843E-4	0.0	0.0	15.701770985228803	0.0
34	4.452508431937843E-4	0.0	0.0	15.96357848102675	0.0
35	4.452508431937843E-4	0.0	0.0	16.266237741687725	0.0
36	4.452508431937843E-4	0.0	0.0	16.544630831394638	0.0
37	4.452508431937843E-4	0.0	0.0	16.820018477909993	0.0
38	4.452508431937843E-4	0.0	0.0	17.111435154780324	0.0
39	4.452508431937843E-4	0.0	0.0	17.409975845141755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.1649703E-6	45.000004	1
TCGATAG	25	3.8901224E-5	45.000004	1
ATGTACG	25	3.8901224E-5	45.000004	1
CGTAACG	30	2.1649703E-6	45.000004	1
TCGAACG	25	3.8901224E-5	45.000004	1
CTATCGC	20	7.0324645E-4	45.0	18
TTGTCGA	20	7.0324645E-4	45.0	29
TCGGTAT	35	1.2115379E-7	45.0	20
TACGTCG	20	7.0324645E-4	45.0	24
TTAACGG	145	0.0	43.44828	2
TACGAAT	105	0.0	42.857143	11
TACGCGG	165	0.0	42.272728	2
AACGGGA	1055	0.0	41.800945	4
ATTAGCG	60	3.6379788E-12	41.250004	1
CGACACG	55	6.002665E-11	40.909092	1
CGGGATT	295	0.0	40.423725	6
TGCAACG	380	0.0	40.263157	1
ACGGGTA	135	0.0	40.0	5
ACTACGG	165	0.0	39.545456	2
CTAGCGG	205	0.0	39.512196	2
>>END_MODULE