FastQCFastQC Report
Sat 18 Jun 2016
SRR3553047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907829
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT40000.4406116129799775TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT34980.3853148555509903No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT33790.372206660064836No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC33050.3640553452247064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10110.11136458518068931No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9310.10255235292108977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG302.1649957E-645.00000445
ATATGCG302.1649957E-645.0000041
CGAACAG253.8901555E-545.01
GCACGTA207.032502E-445.09
CCGTACT406.8121153E-945.044
ATTCACG351.2115561E-745.01
CGTACTC406.8121153E-945.045
GTATCGA351.2115561E-745.037
CGCGTAG207.032502E-445.01
CAATACG253.8901555E-545.01
AACGGGA11750.042.319154
TATACGG650.041.5384642
ATACACG556.002665E-1140.9090921
TTAACGG1500.040.52
GCCGTAC501.0804797E-940.543
GCGTAAG501.0804797E-940.51
CGGGAAT7900.040.443046
TGCAACG4850.040.3608251
CATACGA1350.040.018
ATACGAA1300.039.80769319