Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553047_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 907829 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT | 4000 | 0.4406116129799775 | TruSeq Adapter, Index 21 (95% over 22bp) |
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT | 3498 | 0.3853148555509903 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT | 3379 | 0.372206660064836 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC | 3305 | 0.3640553452247064 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1011 | 0.11136458518068931 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 931 | 0.10255235292108977 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCCTG | 30 | 2.1649957E-6 | 45.000004 | 45 |
ATATGCG | 30 | 2.1649957E-6 | 45.000004 | 1 |
CGAACAG | 25 | 3.8901555E-5 | 45.0 | 1 |
GCACGTA | 20 | 7.032502E-4 | 45.0 | 9 |
CCGTACT | 40 | 6.8121153E-9 | 45.0 | 44 |
ATTCACG | 35 | 1.2115561E-7 | 45.0 | 1 |
CGTACTC | 40 | 6.8121153E-9 | 45.0 | 45 |
GTATCGA | 35 | 1.2115561E-7 | 45.0 | 37 |
CGCGTAG | 20 | 7.032502E-4 | 45.0 | 1 |
CAATACG | 25 | 3.8901555E-5 | 45.0 | 1 |
AACGGGA | 1175 | 0.0 | 42.31915 | 4 |
TATACGG | 65 | 0.0 | 41.538464 | 2 |
ATACACG | 55 | 6.002665E-11 | 40.909092 | 1 |
TTAACGG | 150 | 0.0 | 40.5 | 2 |
GCCGTAC | 50 | 1.0804797E-9 | 40.5 | 43 |
GCGTAAG | 50 | 1.0804797E-9 | 40.5 | 1 |
CGGGAAT | 790 | 0.0 | 40.44304 | 6 |
TGCAACG | 485 | 0.0 | 40.360825 | 1 |
CATACGA | 135 | 0.0 | 40.0 | 18 |
ATACGAA | 130 | 0.0 | 39.807693 | 19 |