##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907829
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879109391746685	33.0	31.0	34.0	30.0	34.0
2	32.08209806031753	33.0	31.0	34.0	30.0	34.0
3	32.209558187720376	34.0	31.0	34.0	30.0	34.0
4	35.86302156022775	37.0	35.0	37.0	35.0	37.0
5	35.65023148632617	37.0	35.0	37.0	33.0	37.0
6	35.67266632813008	37.0	35.0	37.0	33.0	37.0
7	35.753676077763544	37.0	35.0	37.0	35.0	37.0
8	35.794916223209434	37.0	35.0	37.0	35.0	37.0
9	37.497084803415625	39.0	37.0	39.0	35.0	39.0
10	37.05637735740982	39.0	37.0	39.0	33.0	39.0
11	37.037877177309824	39.0	37.0	39.0	33.0	39.0
12	36.94546439913243	39.0	37.0	39.0	33.0	39.0
13	36.92246887905101	39.0	37.0	39.0	33.0	39.0
14	38.11623774962025	40.0	37.0	41.0	33.0	41.0
15	38.15189314287162	40.0	37.0	41.0	33.0	41.0
16	38.02799205577262	40.0	37.0	41.0	33.0	41.0
17	37.99781456639962	40.0	37.0	41.0	33.0	41.0
18	37.94614955019062	40.0	37.0	41.0	33.0	41.0
19	38.00867233807248	40.0	37.0	41.0	33.0	41.0
20	37.94451047499033	40.0	37.0	41.0	33.0	41.0
21	37.91885145770844	40.0	37.0	41.0	33.0	41.0
22	37.90126774976345	40.0	37.0	41.0	33.0	41.0
23	37.76550209345593	40.0	37.0	41.0	33.0	41.0
24	37.63694594466579	40.0	36.0	41.0	32.0	41.0
25	37.68179910533812	40.0	36.0	41.0	33.0	41.0
26	37.624834632954006	40.0	36.0	41.0	33.0	41.0
27	37.567350238866574	40.0	36.0	41.0	33.0	41.0
28	37.47879060924469	40.0	36.0	41.0	32.0	41.0
29	37.30060286683946	39.0	36.0	41.0	32.0	41.0
30	37.393123594862026	39.0	36.0	41.0	32.0	41.0
31	37.277012521080515	39.0	36.0	41.0	32.0	41.0
32	37.2357139945959	39.0	36.0	41.0	32.0	41.0
33	37.177423281256715	39.0	36.0	41.0	32.0	41.0
34	37.012893397324824	39.0	35.0	41.0	31.0	41.0
35	36.97535438942796	39.0	35.0	41.0	31.0	41.0
36	36.85642450285241	39.0	35.0	41.0	31.0	41.0
37	36.82865165135725	39.0	35.0	41.0	31.0	41.0
38	36.74788423811092	39.0	35.0	41.0	31.0	41.0
39	36.65309986792667	39.0	35.0	40.0	31.0	41.0
40	36.56676092083421	39.0	35.0	40.0	30.0	41.0
41	36.45262158402078	39.0	35.0	40.0	30.0	41.0
42	36.407366365251605	39.0	35.0	40.0	30.0	41.0
43	36.32530465539215	38.0	35.0	40.0	30.0	41.0
44	36.25290665973438	38.0	35.0	40.0	30.0	41.0
45	36.07699908242632	38.0	35.0	40.0	30.0	41.0
46	35.929831499103905	38.0	35.0	40.0	29.0	41.0
47	35.7875282679888	38.0	34.0	40.0	29.0	41.0
48	35.67537498802087	38.0	34.0	40.0	29.0	41.0
49	35.52483011668497	38.0	34.0	40.0	28.0	41.0
50	35.443491009870804	37.0	34.0	40.0	28.0	41.0
51	34.15834589994371	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	9.0
11	10.0
12	20.0
13	24.0
14	40.0
15	50.0
16	92.0
17	164.0
18	309.0
19	540.0
20	962.0
21	1399.0
22	2039.0
23	2796.0
24	3592.0
25	4626.0
26	5831.0
27	7332.0
28	9310.0
29	12064.0
30	15656.0
31	20606.0
32	26847.0
33	37023.0
34	57033.0
35	70914.0
36	84737.0
37	124175.0
38	191970.0
39	227590.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.508455887617604	31.58105766614638	20.20116123190601	26.70932521433001
2	24.96119863983195	29.577486509023178	19.31586234852599	26.145452502618888
3	21.494576621808733	29.328651100592733	21.124462866905553	28.05230941069298
4	21.51925087213561	31.162476633815402	18.822597647794904	28.495674846254087
5	18.78459489617538	33.75173077749224	17.730982376637012	29.732691949695372
6	20.802816389430166	35.34377068809214	20.75985675716462	23.09355616531307
7	85.0503784302991	5.374029690613541	5.272028102208676	4.303563776878685
8	86.12492000145402	3.966495892949003	4.360622980759592	5.547961124837387
9	79.61895907709491	6.885107217328374	8.02816389430168	5.467769811275031
10	36.772123384469985	30.993612233140823	13.922335594038085	18.31192878835111
11	28.12787430231905	28.991913675372782	23.11206185305823	19.768150169249935
12	26.596528641407136	24.653211122358947	28.807077103727686	19.94318313250623
13	25.69525758705659	25.44245667410933	26.65314723367506	22.20913850515901
14	22.761885773642394	26.446940998800432	29.048532267640713	21.74264095991646
15	21.331880783715874	27.5282018970533	27.672612353207487	23.467304966023335
16	23.481074078928962	27.699820120309	26.836661970481224	21.98244383028081
17	22.670899475562027	27.182321780864015	26.85395597629069	23.292822767283266
18	24.651668981713517	26.14247837423127	25.939246267744252	23.266606376310957
19	24.116766483555825	28.337495277194275	25.69173269415275	21.85400554509715
20	25.782829145136365	27.3641842241215	24.98455105531989	21.868435575422243
21	25.050312338557152	27.250836886682407	25.258611478593433	22.440239296167007
22	24.230774738414393	26.07076883421878	25.578165050907163	24.12029137645966
23	23.891283490613322	26.851863071129035	24.926170016600043	24.330683421657604
24	21.692080777327007	27.42697137897115	26.42325812460276	24.457689719099083
25	24.486439626846025	27.028768633740498	24.35976378811428	24.1250279512992
26	25.11442132824574	26.823994386608053	24.007604956440034	24.053979328706177
27	22.97459102980848	25.696138810282555	25.83724467933939	25.49202548056958
28	24.348197733273558	27.410999208000625	25.421527622492786	22.819275436233035
29	23.372463316329398	29.259254771548388	24.594059013316386	22.77422289880583
30	25.111667505664613	25.271609521176348	26.13807225810147	23.478650715057572
31	25.098228851468722	28.18041723716691	23.37697958536244	23.344374326001923
32	24.590754426219036	26.692031208520543	25.170158697287704	23.547055667972714
33	25.368323770225448	26.065151036153285	24.743646655923087	23.822878537698177
34	23.02184662530058	28.01254421262154	26.401447849760252	22.56416131231763
35	25.357418632804198	27.121957989885757	26.082334889059506	21.43828848825054
36	23.383478606653895	29.8113411226123	25.622776976721383	21.18240329401242
37	24.21810715454122	28.564410257878965	24.635256199130012	22.582226388449808
38	24.868449895299666	28.522772460452355	25.2935299489221	21.31524769532588
39	24.038668075155122	25.947177276777893	25.752316790937503	24.261837857129482
40	27.204682820222754	25.052184937912315	25.544898874127174	22.198233367737757
41	23.368938423425554	24.708507879787934	28.3005940546072	23.62195964217931
42	26.355734394913576	25.321729092152818	25.067826650173103	23.254709862760496
43	23.41949860601501	25.54170443993307	26.781805824665216	24.2569911293867
44	24.694628613979063	24.263710456484645	25.99289073162457	25.048770197911725
45	26.277195374899897	24.53942317330687	26.071539904541496	23.11184154725174
46	22.61229813103569	24.636578033968952	28.844418937927735	23.90670489706762
47	23.81913333898785	26.17398210455934	26.277856292319367	23.729028264133444
48	23.402424906012033	25.441244992173633	28.22954543201418	22.92678466980015
49	24.3120675810092	25.0041582720975	26.81936796467176	23.864406182221543
50	22.55006174070227	25.481120343148323	26.58242906979178	25.386388846357626
51	23.385020747299325	23.98039718933852	28.29717931460661	24.337402748755547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1333.0
1	1084.0
2	835.0
3	615.5
4	396.0
5	399.5
6	403.0
7	442.5
8	482.0
9	588.5
10	695.0
11	713.5
12	732.0
13	831.5
14	931.0
15	992.0
16	1053.0
17	1112.0
18	1171.0
19	1291.5
20	1412.0
21	1754.0
22	2096.0
23	3062.5
24	4029.0
25	4032.5
26	5311.0
27	6586.0
28	8540.0
29	10494.0
30	11656.5
31	12819.0
32	15160.0
33	17501.0
34	19948.0
35	22395.0
36	24192.0
37	25989.0
38	28366.0
39	30743.0
40	34784.0
41	38825.0
42	47395.0
43	55965.0
44	61502.0
45	67039.0
46	72479.0
47	77919.0
48	75827.5
49	73736.0
50	71079.0
51	68422.0
52	63722.5
53	59023.0
54	56055.0
55	53087.0
56	50036.0
57	46985.0
58	46983.0
59	46981.0
60	44110.0
61	41239.0
62	38432.0
63	35625.0
64	30679.0
65	25733.0
66	22915.0
67	20097.0
68	17257.0
69	14417.0
70	13024.0
71	11631.0
72	10395.0
73	9159.0
74	7563.5
75	4992.0
76	4016.0
77	3375.5
78	2735.0
79	2176.0
80	1617.0
81	1177.0
82	737.0
83	541.0
84	345.0
85	293.0
86	241.0
87	150.0
88	59.0
89	44.0
90	29.0
91	37.0
92	45.0
93	28.5
94	12.0
95	9.0
96	6.0
97	3.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	907829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.96803153957934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85710192663724	19.698596153320004
2	9.277800490579915	4.818524315146078
3	3.180557907285243	2.4777848414952492
4	1.616482276499261	1.6790745095721527
5	1.0064121338992114	1.3067271017455033
6	0.706791063982019	1.1012383584826746
7	0.49481152383620286	0.8994496879988082
8	0.4543668079134639	0.9439209278747853
9	0.36703604864383244	0.8578083318611056
>10	5.550454558571425	35.783692345866164
>50	1.3697971755648208	23.64951177211587
>100	0.11197717937148247	4.29281823116458
>500	0.004273938143949712	0.8046467637721556
>1k	0.002136969071974856	1.6862066595848773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	4000	0.4406116129799775	TruSeq Adapter, Index 21 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	3498	0.3853148555509903	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGT	3379	0.372206660064836	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	3305	0.3640553452247064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1011	0.11136458518068931	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	931	0.10255235292108977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5059322846042592	0.0
2	0.0	0.0	0.0	0.7271193143202079	0.0
3	0.0	0.0	0.0	1.0560358834097612	0.0
4	0.0	0.0	0.0	2.409594758484252	0.0
5	0.0	0.0	0.0	2.622740626263316	0.0
6	0.0	0.0	0.0	3.644518956763884	0.0
7	0.0	0.0	0.0	4.380560656246937	0.0
8	0.0	0.0	0.0	5.00986419248559	0.0
9	0.0	0.0	0.0	6.111613530742023	0.0
10	0.0	0.0	0.0	7.289478525140748	0.0
11	0.0	0.0	0.0	8.62210834859869	0.0
12	0.0	0.0	0.0	9.132336596429504	0.0
13	0.0	0.0	0.0	9.379409558408026	0.0
14	0.0	0.0	0.0	9.662612672650907	0.0
15	0.0	0.0	0.0	9.897678968175725	0.0
16	0.0	0.0	0.0	10.317912294055379	0.0
17	0.0	0.0	0.0	10.812608982528648	0.0
18	0.0	0.0	0.0	11.511969765231118	0.0
19	0.0	0.0	0.0	11.813678567219156	1.1015290324499438E-4
20	1.1015290324499438E-4	0.0	0.0	12.176852689217903	1.1015290324499438E-4
21	1.1015290324499438E-4	0.0	0.0	12.574724975738823	1.1015290324499438E-4
22	1.1015290324499438E-4	0.0	0.0	12.974029250001928	1.1015290324499438E-4
23	1.1015290324499438E-4	0.0	0.0	13.376197499749402	1.1015290324499438E-4
24	1.1015290324499438E-4	0.0	0.0	13.685396699158101	1.1015290324499438E-4
25	1.1015290324499438E-4	0.0	0.0	13.955051006301847	1.1015290324499438E-4
26	1.1015290324499438E-4	0.0	0.0	14.20697069602315	1.1015290324499438E-4
27	1.1015290324499438E-4	0.0	0.0	14.510001332850129	1.1015290324499438E-4
28	1.1015290324499438E-4	0.0	0.0	14.789128789672946	1.1015290324499438E-4
29	1.1015290324499438E-4	0.0	0.0	15.069467928431456	1.1015290324499438E-4
30	1.1015290324499438E-4	0.0	0.0	15.538829449158376	1.1015290324499438E-4
31	1.1015290324499438E-4	0.0	0.0	15.812008649205962	1.1015290324499438E-4
32	1.1015290324499438E-4	0.0	0.0	16.114158062806982	1.1015290324499438E-4
33	1.1015290324499438E-4	0.0	0.0	16.413333348020387	1.1015290324499438E-4
34	1.1015290324499438E-4	0.0	0.0	16.67847138613109	1.1015290324499438E-4
35	1.1015290324499438E-4	0.0	0.0	16.987119821023562	1.1015290324499438E-4
36	1.1015290324499438E-4	0.0	0.0	17.26316299655552	1.1015290324499438E-4
37	1.1015290324499438E-4	0.0	0.0	17.556390024993693	1.1015290324499438E-4
38	1.1015290324499438E-4	0.0	0.0	17.849837359238357	1.1015290324499438E-4
39	1.1015290324499438E-4	0.0	0.0	18.154299983807523	1.1015290324499438E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTG	30	2.1649957E-6	45.000004	45
ATATGCG	30	2.1649957E-6	45.000004	1
CGAACAG	25	3.8901555E-5	45.0	1
GCACGTA	20	7.032502E-4	45.0	9
CCGTACT	40	6.8121153E-9	45.0	44
ATTCACG	35	1.2115561E-7	45.0	1
CGTACTC	40	6.8121153E-9	45.0	45
GTATCGA	35	1.2115561E-7	45.0	37
CGCGTAG	20	7.032502E-4	45.0	1
CAATACG	25	3.8901555E-5	45.0	1
AACGGGA	1175	0.0	42.31915	4
TATACGG	65	0.0	41.538464	2
ATACACG	55	6.002665E-11	40.909092	1
TTAACGG	150	0.0	40.5	2
GCCGTAC	50	1.0804797E-9	40.5	43
GCGTAAG	50	1.0804797E-9	40.5	1
CGGGAAT	790	0.0	40.44304	6
TGCAACG	485	0.0	40.360825	1
CATACGA	135	0.0	40.0	18
ATACGAA	130	0.0	39.807693	19
>>END_MODULE