FastQCFastQC Report
Sat 18 Jun 2016
SRR3553045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486255
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC28900.5943383615592642No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT28250.5809708897594883No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT26330.5414854345970735No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT23260.4783498370196707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13900.2858582431028987No Hit
AATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT6450.13264645093623717No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6350.13058991681319473No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT5600.11516591089037645No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT5590.11496025747807222No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC5480.11269806994272553No Hit
AACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG5090.10467758686286002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA207.029266E-445.00000418
CTTCGAT207.029266E-445.00000419
GATACGA207.029266E-445.00000440
AGTCCGT207.029266E-445.00000433
GGTCGCT207.029266E-445.0000048
CGAAAAC207.029266E-445.00000426
CTATACG207.029266E-445.0000041
GACCGTC207.029266E-445.00000416
CGCGACT207.029266E-445.00000428
CGTTATG207.029266E-445.00000421
ATGTACC207.029266E-445.00000443
TAGCACG406.8012014E-945.0000041
TGCGACG207.029266E-445.0000041
CGCTAAG207.029266E-445.0000041
CGAATCC207.029266E-445.00000430
CATACGT207.029266E-445.00000422
CATACGA207.029266E-445.00000418
AATTCCG207.029266E-445.0000041
CTCGACG207.029266E-445.00000421
CGTCGAC207.029266E-445.00000437