##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486255
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80491511655407	31.0	31.0	34.0	30.0	34.0
2	32.009207103268864	33.0	31.0	34.0	30.0	34.0
3	32.143284901954736	34.0	31.0	34.0	30.0	34.0
4	35.78407625628528	37.0	35.0	37.0	35.0	37.0
5	35.56931651088421	37.0	35.0	37.0	33.0	37.0
6	35.58931836176492	37.0	35.0	37.0	33.0	37.0
7	35.61745997470463	37.0	35.0	37.0	35.0	37.0
8	35.673202332109696	37.0	35.0	37.0	35.0	37.0
9	37.375346268932965	39.0	37.0	39.0	35.0	39.0
10	36.956950571202356	39.0	37.0	39.0	33.0	39.0
11	36.95090847394886	39.0	37.0	39.0	33.0	39.0
12	36.850226732887066	39.0	37.0	39.0	33.0	39.0
13	36.82591027341621	39.0	37.0	39.0	33.0	39.0
14	37.98626235205808	40.0	37.0	41.0	33.0	41.0
15	38.01118137602698	40.0	37.0	41.0	33.0	41.0
16	37.90385497321364	40.0	37.0	41.0	33.0	41.0
17	37.882166764352036	40.0	37.0	41.0	33.0	41.0
18	37.799773781246465	40.0	37.0	41.0	33.0	41.0
19	37.83150815929913	40.0	36.0	41.0	33.0	41.0
20	37.77786346669957	40.0	36.0	41.0	33.0	41.0
21	37.75030796598492	40.0	36.0	41.0	33.0	41.0
22	37.73787621721113	40.0	36.0	41.0	33.0	41.0
23	37.591588775436755	39.0	36.0	41.0	33.0	41.0
24	37.45753154209211	39.0	36.0	41.0	32.0	41.0
25	37.471978694306486	39.0	36.0	41.0	32.0	41.0
26	37.41219936041789	39.0	36.0	41.0	32.0	41.0
27	37.328276315924775	39.0	36.0	41.0	32.0	41.0
28	37.18991886972885	39.0	36.0	41.0	31.0	41.0
29	37.07832721514432	39.0	35.0	41.0	31.0	41.0
30	37.10310845132698	39.0	35.0	41.0	31.0	41.0
31	36.993273076883526	39.0	35.0	41.0	31.0	41.0
32	36.927819765349454	39.0	35.0	41.0	31.0	41.0
33	36.829439285971354	39.0	35.0	41.0	31.0	41.0
34	36.76808670347863	39.0	35.0	41.0	31.0	41.0
35	36.689039701391245	39.0	35.0	41.0	31.0	41.0
36	36.56905944411883	39.0	35.0	40.0	30.0	41.0
37	36.484387821204926	39.0	35.0	40.0	30.0	41.0
38	36.35342155864721	39.0	35.0	40.0	30.0	41.0
39	36.26745431923579	39.0	35.0	40.0	30.0	41.0
40	36.16429445455574	38.0	35.0	40.0	30.0	41.0
41	36.068519603911525	38.0	35.0	40.0	30.0	41.0
42	35.97852772722131	38.0	35.0	40.0	30.0	41.0
43	35.87943980010488	38.0	35.0	40.0	29.0	41.0
44	35.77959918149942	38.0	35.0	40.0	29.0	41.0
45	35.59577382237715	38.0	34.0	40.0	28.0	41.0
46	35.413093952761415	38.0	34.0	40.0	28.0	41.0
47	35.29992699303863	37.0	34.0	40.0	27.0	41.0
48	35.232413034313275	37.0	34.0	40.0	27.0	41.0
49	35.0722563264131	37.0	34.0	40.0	27.0	41.0
50	34.977884032040805	37.0	34.0	40.0	27.0	41.0
51	33.65372489743036	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	5.0
13	9.0
14	24.0
15	29.0
16	60.0
17	113.0
18	231.0
19	465.0
20	755.0
21	1107.0
22	1628.0
23	2146.0
24	2545.0
25	3096.0
26	3595.0
27	4391.0
28	5413.0
29	6966.0
30	9076.0
31	11756.0
32	15561.0
33	20867.0
34	32065.0
35	39586.0
36	47382.0
37	67865.0
38	99556.0
39	109935.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.629505095063287	32.148564025048586	20.026734943599553	27.195195936288574
2	23.73055289919898	31.389497280233623	19.25080461897564	25.629145201591758
3	21.046981522040905	29.833523562739718	22.19822932412006	26.92126559109932
4	22.18794665350485	30.986005285292695	18.767519100060667	28.05852896114179
5	18.998467882078334	34.857019465095476	16.828001768619348	29.31651088420685
6	20.061284716866666	37.022755550071466	20.355780403286342	22.56017932977553
7	82.29180162671848	7.23961707334629	5.813205005604055	4.655376294331163
8	83.60366474380726	5.027814624014149	4.765812176738542	6.602708455440047
9	77.88917337610924	7.999300778398166	8.555181951856536	5.556343893636055
10	36.655869862520696	31.551552169129366	13.107114579798665	18.685463388551273
11	26.905841585176503	30.044729617176174	22.666296490524516	20.383132307122807
12	26.876844454041603	24.52581464457949	28.819857893492095	19.777483007886808
13	25.395111618389528	25.608168553536725	26.267699046796434	22.729020781277313
14	21.967074888690092	26.92558431275771	28.997748095135268	22.10959270341693
15	20.49048338834562	28.308192203679138	27.502442134271117	23.69888227370413
16	22.33293230917934	26.94224223915435	26.675304109983443	24.049521341682862
17	21.636384201704868	26.655150075577627	26.822346299780982	24.886119422936524
18	23.755025655263186	25.853307421003386	25.794284891672064	24.597382032061365
19	22.986704506894533	28.7423265570534	25.91623736516848	22.35473157088359
20	24.72056842603161	28.50459121242969	24.70699530081953	22.067845060719172
21	24.552549588179044	27.09771621885636	25.495470483594	22.8542637093706
22	23.787518894407256	25.68014724784321	24.208697082806346	26.32363677494319
23	23.190507038488036	27.271287698841146	23.227935959527407	26.310269303143414
24	22.324089212450257	29.13553588137911	24.471933450555778	24.068441455614852
25	22.9124636250527	27.981820238352306	23.76284048493075	25.342875651664247
26	25.323955537732264	27.285066477465524	22.96860700661176	24.422370978190454
27	21.93170250177376	26.367646605176297	25.201180450586623	26.49947044246332
28	22.842952771693863	28.91281323585362	25.430278351893556	22.813955640558966
29	24.389672085634082	29.06581937460797	23.014467717555604	23.53004082220234
30	24.200059639489567	27.612466709853884	23.29621289241242	24.891260758244133
31	26.05567037871076	29.741390834027413	20.51475049099752	23.688188296264308
32	25.27110261077007	27.036842808814303	22.430206373199248	25.26184820721638
33	24.69506740290588	27.852875548837545	23.088297292572825	24.363759755683745
34	22.440283390402154	28.75240357425631	23.301765534544632	25.505547500796904
35	25.429867045068942	27.459871877924137	23.40438658728445	23.70587448972247
36	25.719427049593317	28.70469198260172	22.61262094991311	22.963260017891844
37	24.0805750069408	29.677843929625404	20.851199473527267	25.39038158990653
38	25.87469537588302	28.33883456211247	23.646646306978848	22.139823755025656
39	25.468324233169838	24.890026837770304	23.248912607582444	26.392736321477418
40	26.31499933162641	25.243339400108994	23.760989604220008	24.680671664044585
41	24.44190805235936	23.46875610533568	25.621330371924195	26.468005470380767
42	26.087546657617917	24.934242321415717	23.535182157509947	25.44302886345642
43	24.65311410679582	24.045613926849082	25.13064133016627	26.17063063618883
44	23.84016616795714	23.236984709668796	24.64385970324213	28.27898941913194
45	26.141222198229325	24.367050210280613	25.810325857831796	23.681401733658266
46	22.67596220090282	25.116245591304974	27.952000493568192	24.255791714224017
47	24.131988360016862	25.760969038878777	24.414144841698285	25.692897759406076
48	23.123258372664548	25.017531953398937	27.600950118764843	24.25825955517167
49	24.448900268377702	23.8720424468643	26.194075125191514	25.48498215956648
50	22.751025696393867	23.59811210167505	26.167751488416574	27.483110713514513
51	23.374566842500336	23.463820423440378	27.47982025891765	25.681792475141645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1706.0
1	1359.0
2	1012.0
3	729.0
4	446.0
5	442.5
6	439.0
7	453.0
8	467.0
9	540.0
10	613.0
11	651.0
12	689.0
13	748.0
14	807.0
15	806.5
16	806.0
17	796.5
18	787.0
19	933.5
20	1080.0
21	1151.5
22	1223.0
23	1457.5
24	1692.0
25	1937.5
26	2605.5
27	3028.0
28	3475.5
29	3923.0
30	5120.0
31	6317.0
32	8122.5
33	9928.0
34	9867.5
35	9807.0
36	11074.5
37	12342.0
38	12687.5
39	13033.0
40	13773.0
41	14513.0
42	16780.0
43	19047.0
44	21743.5
45	24440.0
46	29905.5
47	35371.0
48	39431.0
49	43491.0
50	45717.0
51	47943.0
52	44872.5
53	41802.0
54	38531.0
55	35260.0
56	32033.5
57	28807.0
58	28219.5
59	27632.0
60	25168.5
61	22705.0
62	20642.0
63	18579.0
64	16460.5
65	14342.0
66	13207.5
67	12073.0
68	10424.0
69	8775.0
70	7352.5
71	5930.0
72	5468.0
73	5006.0
74	4200.5
75	2852.0
76	2309.0
77	1690.5
78	1072.0
79	899.0
80	726.0
81	537.5
82	349.0
83	247.0
84	145.0
85	144.5
86	144.0
87	93.0
88	42.0
89	30.0
90	18.0
91	12.5
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.285699194735514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26060117986722	21.081095233982847
2	9.354531602985713	5.104898708534303
3	3.3203075872756957	2.71790742181208
4	1.5890995843988724	1.7343877300154737
5	0.8958472838232763	1.2221909755411384
6	0.6366073751102009	1.0422166405444258
7	0.4559948577640186	0.8709496966306651
8	0.3112692562063004	0.6794559434731338
9	0.27717538569117867	0.680663177833887
>10	4.341569212116707	30.18102726383493
>50	1.416897265796426	26.323183334873207
>100	0.1317691738367601	5.155079855236373
>500	0.004543764615060693	0.7141236163098005
>1k	0.0037864705125505776	2.4928204013777298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	2890	0.5943383615592642	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	2825	0.5809708897594883	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT	2633	0.5414854345970735	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	2326	0.4783498370196707	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1390	0.2858582431028987	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	645	0.13264645093623717	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	635	0.13058991681319473	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT	560	0.11516591089037645	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	559	0.11496025747807222	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	548	0.11269806994272553	No Hit
AACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	509	0.10467758686286002	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6595304932597094	0.0
2	0.0	0.0	0.0	0.9017902129541084	0.0
3	0.0	0.0	0.0	1.2705267812156174	0.0
4	0.0	0.0	0.0	2.896011352068359	0.0
5	0.0	0.0	0.0	3.141561526359626	0.0
6	0.0	0.0	0.0	4.358207113551532	0.0
7	0.0	0.0	0.0	5.239226331862912	0.0
8	0.0	0.0	0.0	6.02667324757586	0.0
9	0.0	0.0	0.0	7.382957501722347	0.0
10	0.0	0.0	0.0	8.731632579613578	0.0
11	0.0	0.0	0.0	10.476396129602781	0.0
12	0.0	0.0	0.0	11.102405116656898	0.0
13	0.0	0.0	0.0	11.403481712270311	0.0
14	0.0	0.0	0.0	11.781061377260903	0.0
15	0.0	0.0	0.0	12.044709051834943	0.0
16	0.0	0.0	0.0	12.559870849657074	0.0
17	0.0	0.0	0.0	13.123155545958396	0.0
18	0.0	0.0	0.0	14.077387379050087	0.0
19	0.0	0.0	0.0	14.420211617361261	0.0
20	2.0565341230424365E-4	0.0	0.0	14.791827333395029	0.0
21	2.0565341230424365E-4	0.0	0.0	15.175576600754749	0.0
22	2.0565341230424365E-4	0.0	0.0	15.548220583850037	0.0
23	2.0565341230424365E-4	0.0	0.0	15.950273004904833	0.0
24	2.0565341230424365E-4	0.0	0.0	16.2490874129829	0.0
25	2.0565341230424365E-4	0.0	0.0	16.517259462627635	0.0
26	2.0565341230424365E-4	0.0	0.0	16.771652733647983	0.0
27	2.0565341230424365E-4	0.0	0.0	17.062035351821574	0.0
28	2.0565341230424365E-4	0.0	0.0	17.365785441794944	0.0
29	2.0565341230424365E-4	0.0	0.0	17.643417548405672	0.0
30	2.0565341230424365E-4	0.0	0.0	18.07714059495532	0.0
31	2.0565341230424365E-4	0.0	0.0	18.3377034683448	0.0
32	2.0565341230424365E-4	0.0	0.0	18.649062734573423	0.0
33	2.0565341230424365E-4	0.0	0.0	18.94931671653762	0.0
34	2.0565341230424365E-4	0.0	0.0	19.218517033243874	0.0
35	2.0565341230424365E-4	0.0	0.0	19.523912350515676	0.0
36	2.0565341230424365E-4	0.0	0.0	19.78488653072976	0.0
37	2.0565341230424365E-4	0.0	0.0	20.05429250084832	0.0
38	2.0565341230424365E-4	0.0	0.0	20.328634152862183	0.0
39	2.0565341230424365E-4	0.0	0.0	20.609762367482084	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	20	7.029266E-4	45.000004	18
CTTCGAT	20	7.029266E-4	45.000004	19
GATACGA	20	7.029266E-4	45.000004	40
AGTCCGT	20	7.029266E-4	45.000004	33
GGTCGCT	20	7.029266E-4	45.000004	8
CGAAAAC	20	7.029266E-4	45.000004	26
CTATACG	20	7.029266E-4	45.000004	1
GACCGTC	20	7.029266E-4	45.000004	16
CGCGACT	20	7.029266E-4	45.000004	28
CGTTATG	20	7.029266E-4	45.000004	21
ATGTACC	20	7.029266E-4	45.000004	43
TAGCACG	40	6.8012014E-9	45.000004	1
TGCGACG	20	7.029266E-4	45.000004	1
CGCTAAG	20	7.029266E-4	45.000004	1
CGAATCC	20	7.029266E-4	45.000004	30
CATACGT	20	7.029266E-4	45.000004	22
CATACGA	20	7.029266E-4	45.000004	18
AATTCCG	20	7.029266E-4	45.000004	1
CTCGACG	20	7.029266E-4	45.000004	21
CGTCGAC	20	7.029266E-4	45.000004	37
>>END_MODULE