##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726721
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783383169056627	31.0	31.0	34.0	30.0	34.0
2	32.00148750345731	33.0	31.0	34.0	30.0	34.0
3	32.15171021616273	34.0	31.0	34.0	30.0	34.0
4	35.80104882066158	37.0	35.0	37.0	35.0	37.0
5	35.551314741145504	37.0	35.0	37.0	33.0	37.0
6	34.87550380407337	37.0	35.0	37.0	32.0	37.0
7	35.26103965620919	37.0	35.0	37.0	33.0	37.0
8	35.66183996334219	37.0	35.0	37.0	33.0	37.0
9	37.348711541293014	39.0	37.0	39.0	34.0	39.0
10	36.942588696349766	39.0	37.0	39.0	33.0	39.0
11	36.93775190203668	39.0	37.0	39.0	33.0	39.0
12	36.76963236235089	39.0	35.0	39.0	33.0	39.0
13	36.77110197723748	39.0	35.0	39.0	33.0	39.0
14	37.90940539766981	40.0	37.0	41.0	33.0	41.0
15	37.97560411767377	40.0	37.0	41.0	33.0	41.0
16	37.83527928875043	40.0	37.0	41.0	33.0	41.0
17	37.81744025561392	40.0	37.0	41.0	33.0	41.0
18	37.77600757374563	40.0	37.0	41.0	33.0	41.0
19	37.766277567319506	40.0	36.0	41.0	33.0	41.0
20	37.707080158685386	40.0	36.0	41.0	33.0	41.0
21	37.73463956594071	40.0	36.0	41.0	33.0	41.0
22	37.70708841494879	40.0	36.0	41.0	33.0	41.0
23	37.524578208143154	39.0	36.0	41.0	32.0	41.0
24	37.44923430037112	39.0	36.0	41.0	32.0	41.0
25	37.492663621940196	39.0	36.0	41.0	32.0	41.0
26	37.366801014419565	39.0	36.0	41.0	32.0	41.0
27	37.25774403106557	39.0	36.0	41.0	32.0	41.0
28	37.22870124848463	39.0	36.0	41.0	32.0	41.0
29	36.9937775294783	39.0	35.0	41.0	31.0	41.0
30	37.08012428428517	39.0	35.0	41.0	31.0	41.0
31	37.03794165849067	39.0	35.0	41.0	31.0	41.0
32	37.00515053232258	39.0	35.0	41.0	31.0	41.0
33	36.72817215960458	39.0	35.0	41.0	30.0	41.0
34	36.69538378552429	39.0	35.0	41.0	30.0	41.0
35	36.67623338254984	39.0	35.0	41.0	30.0	41.0
36	36.586527704579886	39.0	35.0	40.0	30.0	41.0
37	36.580437334272716	39.0	35.0	40.0	30.0	41.0
38	36.3709539149137	39.0	35.0	40.0	30.0	41.0
39	36.31465032660402	39.0	35.0	40.0	30.0	41.0
40	36.191279734588655	38.0	35.0	40.0	30.0	41.0
41	36.18663283433395	38.0	35.0	40.0	30.0	41.0
42	36.1696029150114	38.0	35.0	40.0	30.0	41.0
43	36.06519420795601	38.0	35.0	40.0	30.0	41.0
44	35.99770063064092	38.0	35.0	40.0	30.0	41.0
45	35.76914799489763	38.0	35.0	40.0	29.0	41.0
46	35.58599104745838	38.0	34.0	40.0	28.0	41.0
47	35.43826310234602	38.0	34.0	40.0	28.0	41.0
48	35.376780084791825	37.0	34.0	40.0	28.0	41.0
49	35.28795507491871	37.0	34.0	40.0	27.0	41.0
50	35.20149823659974	37.0	34.0	40.0	27.0	41.0
51	33.91533752292833	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	1.0
10	1.0
11	1.0
12	8.0
13	10.0
14	24.0
15	37.0
16	60.0
17	141.0
18	280.0
19	596.0
20	1151.0
21	1667.0
22	2231.0
23	2898.0
24	3568.0
25	4522.0
26	5237.0
27	6580.0
28	8171.0
29	10913.0
30	13721.0
31	18402.0
32	23878.0
33	32509.0
34	48807.0
35	59754.0
36	68646.0
37	98646.0
38	144616.0
39	169586.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.895117933842563	34.38940115945459	19.80581268464789	25.909668222054954
2	24.62017748214239	31.98559006826554	18.714334662133062	24.67989778745901
3	21.617512085105563	28.91426008055361	21.53646309931872	27.931764735022107
4	22.698807382750736	29.543662561010347	18.886340149796137	28.871189906442773
5	19.878192593856514	35.03407772721581	17.404340868091055	27.68338881083662
6	19.712792116919697	36.867105808143705	22.586522200404282	20.83357987453232
7	83.15598420852018	6.994843963501811	5.641091973398319	4.2080798545796805
8	82.99952801694185	5.663108675819193	4.340868091055577	6.996495216183376
9	77.68098073400934	9.137481922223246	7.5740208415609285	5.607516502206487
10	35.87497815530307	32.459912401045244	14.823157718023836	16.841951725627855
11	29.097961941377775	28.881923048872952	22.47919077610252	19.54092423364675
12	26.499853451324512	25.87210222354934	28.0821663334347	19.545877991691444
13	26.250239087627854	25.119819022706103	26.798042164737225	21.831899724928824
14	20.672307529299413	27.974009282792156	29.14089451109848	22.212788676809943
15	20.67519722149215	27.399648558387607	27.116458723499115	24.808695496621127
16	23.79345030623857	27.40074939350865	26.881155216376023	21.924645083876758
17	23.056165983919552	26.422932597241584	26.464351518670853	24.056549900168015
18	23.892387862742375	25.828619236268114	25.453647273162606	24.825345627826913
19	23.821934414995578	28.128951826079057	26.43683064064476	21.612283118280608
20	25.006983422799124	29.235841540288504	23.84876727107102	21.90840776584136
21	24.971756698925724	26.855973612982147	24.866902153646308	23.30536753444582
22	24.156725896182994	25.642853309591988	24.659945150890096	25.540475643334926
23	22.28626942113961	28.300682104961876	24.299696857528545	25.113351616369968
24	20.70106684683668	29.17061705936666	25.55740098332097	24.570915110475685
25	23.03538772100985	27.478220665152104	24.28139547364119	25.204996140196855
26	25.123671945629756	27.787692938555512	22.87136328797434	24.217271827840396
27	23.268214349110593	26.63332970975106	23.94453992660182	26.153916014536527
28	22.03225171695878	28.025335720310824	24.431521863273524	25.510890699456873
29	23.062082972695162	30.958786109112026	23.567366293254217	22.411764624938595
30	25.378790484931628	25.42612639513651	24.91492608580184	24.280157034130017
31	25.226324820667077	28.13665767192636	20.8822918286385	25.754725678768054
32	26.381926488982703	25.77825602948036	23.35793241147566	24.481885070061274
33	23.923211246131597	25.517082897012745	24.02889141775179	26.530814439103867
34	22.45235792002708	27.951717371591023	24.57229115437699	25.02363355400491
35	25.119543813925837	25.513092369698963	25.70862820807435	23.658735608300844
36	23.472832077234592	29.97037377480491	24.32240158190007	22.234392566060425
37	25.72734240513209	26.73845946381073	22.30319476112566	25.231003369931514
38	25.36943338640276	27.16283140297308	25.0335410700943	22.434194140529858
39	24.253048969274317	25.451445602920515	25.31040110303679	24.98510432476838
40	25.28411866452187	25.406448967347856	25.648219880807076	23.661212487323198
41	22.599732221856804	25.7878883367895	26.869321238824806	24.743058202528896
42	26.371606159722923	25.40452250588603	22.67169931789504	25.552172016496016
43	23.52264486646182	24.67659528209588	26.008055360998238	25.792704490444063
44	25.154495329018978	23.50117858160147	24.751176861546593	26.593149227832964
45	25.69349172516	24.199658465903696	25.60776419010872	24.499085618827582
46	22.61583193550207	24.092189437211804	28.937240013705395	24.35473861358073
47	23.487555746978554	27.183472061492647	25.150229592924934	24.17874259860387
48	23.20973248330515	26.604983205384187	27.11191777862481	23.07336653268586
49	24.232545915144875	25.047163904717216	25.987277098088533	24.73301308204937
50	22.849897003113988	24.71209721474954	25.763257150956143	26.67474863118033
51	22.749583402708883	23.43677972702041	28.0473524227317	25.76628444753901
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2063.0
1	1712.0
2	1361.0
3	935.0
4	509.0
5	513.5
6	518.0
7	519.5
8	521.0
9	552.5
10	584.0
11	623.0
12	662.0
13	858.0
14	1054.0
15	1097.5
16	1141.0
17	1205.5
18	1270.0
19	1374.5
20	1479.0
21	1763.5
22	2048.0
23	2430.5
24	2813.0
25	3218.0
26	4341.5
27	5060.0
28	6392.5
29	7725.0
30	9983.5
31	12242.0
32	13061.5
33	13881.0
34	14584.0
35	15287.0
36	17022.5
37	18758.0
38	20953.0
39	23148.0
40	23465.0
41	23782.0
42	26379.0
43	28976.0
44	32807.5
45	36639.0
46	47268.5
47	57898.0
48	60245.0
49	62592.0
50	67957.0
51	73322.0
52	68290.5
53	63259.0
54	56287.5
55	49316.0
56	46140.0
57	42964.0
58	39956.5
59	36949.0
60	35454.0
61	33959.0
62	30873.0
63	27787.0
64	23689.0
65	19591.0
66	17406.0
67	15221.0
68	13109.5
69	10998.0
70	9613.0
71	8228.0
72	7162.0
73	6096.0
74	5100.5
75	4129.0
76	4153.0
77	3330.0
78	2507.0
79	1968.0
80	1429.0
81	1094.5
82	760.0
83	507.0
84	254.0
85	181.5
86	109.0
87	81.0
88	53.0
89	33.5
90	14.0
91	10.0
92	6.0
93	4.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726721.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.2659826104271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02442164214641	17.687828718939556
2	10.031507449360424	4.66785755746379
3	3.6123973993284215	2.5213792522418137
4	1.7562683370155083	1.6344523435298612
5	1.0426761523935584	1.212944261494778
6	0.6883615524266936	0.9609244745067632
7	0.46274344544867446	0.7536326668428586
8	0.36198841849870006	0.6737612999973409
9	0.2834621026486607	0.5935521915844956
>10	3.559865216738508	20.667382175254104
>50	1.6277047814567192	27.303016938527296
>100	0.5365330184334531	16.639276641169097
>500	0.00543171784690785	0.9819279455839283
>1k	0.006035242052119833	2.9733053407321153
>5k	6.035242052119834E-4	0.7287581921322163
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC	5190	0.7141667847771016	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGT	4691	0.6455021941020007	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	4301	0.5918364819511202	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	3421	0.47074461863631295	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1700	0.23392746322178662	No Hit
ACCCGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTC	1414	0.1945726076444743	No Hit
ACCCGTGAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC	1197	0.16471245498616388	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	1196	0.1645748505960334	No Hit
ACCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	1119	0.1539793125559878	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT	1117	0.15370410377572685	No Hit
ACCCGTGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAG	1019	0.14021887354294152	No Hit
AACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	992	0.13650355500941902	No Hit
ACCCGTGAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGT	972	0.13375146720680978	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCGTAGTC	946	0.13017375306341772	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTAT	840	0.11558768770958869	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	784	0.10788184186228277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5325289898048907	0.0
2	0.0	0.0	0.0	0.7302664984223657	0.0
3	0.0	0.0	0.0	1.0887259347122211	0.0
4	0.0	0.0	0.0	2.731722352869946	0.0
5	0.0	0.0	0.0	2.9289094439268992	0.0
6	0.0	0.0	0.0	3.9017724821492705	0.0
7	0.0	0.0	0.0	4.64511139763403	0.0
8	0.0	0.0	0.0	5.238599132266716	0.0
9	0.0	0.0	0.0	6.591112682859034	0.0
10	0.0	0.0	0.0	7.763089273600185	0.0
11	0.0	0.0	0.0	9.601071112572775	0.0
12	0.0	0.0	0.0	10.136627398960536	0.0
13	0.0	0.0	0.0	10.416377124095767	0.0
14	0.0	0.0	0.0	10.845702821302812	0.0
15	0.0	0.0	0.0	11.074126108919378	0.0
16	0.0	0.0	0.0	11.635001603091146	0.0
17	0.0	0.0	0.0	12.110011957821502	0.0
18	0.0	0.0	0.0	13.150576355988061	0.0
19	0.0	0.0	0.0	13.453856431835602	0.0
20	0.0	0.0	0.0	13.816031186658979	0.0
21	0.0	0.0	0.0	14.236687807287804	0.0
22	0.0	0.0	0.0	14.596248078698704	0.0
23	0.0	0.0	0.0	14.949065734993209	0.0
24	0.0	0.0	0.0	15.208450010389132	0.0
25	0.0	0.0	0.0	15.629519444188347	0.0
26	0.0	0.0	0.0	15.878858599104746	0.0
27	0.0	0.0	0.0	16.143059028155236	0.0
28	0.0	0.0	0.0	16.494913453718826	0.0
29	0.0	0.0	0.0	16.767920563737665	0.0
30	0.0	0.0	0.0	17.207291381424234	0.0
31	0.0	0.0	0.0	17.448924690493325	0.0
32	0.0	0.0	0.0	17.715189185395772	0.0
33	0.0	0.0	0.0	17.98847150419487	0.0
34	0.0	0.0	0.0	18.233544923017224	0.0
35	0.0	0.0	0.0	18.518661219367544	0.0
36	0.0	0.0	0.0	18.775981428911507	0.0
37	0.0	0.0	0.0	19.028210276020648	0.0
38	0.0	0.0	0.0	19.27865026605809	0.0
39	0.0	0.0	0.0	19.5465660136421	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	35	1.2111013E-7	45.000004	1
TGTCGAA	35	1.2111013E-7	45.000004	39
ATCGCAA	35	1.2111013E-7	45.000004	29
TTGATCG	20	7.0315733E-4	45.0	25
CTGTCGT	20	7.0315733E-4	45.0	41
GGTACGA	40	6.8084773E-9	45.0	9
TCCGCGC	20	7.0315733E-4	45.0	44
CGTATAA	20	7.0315733E-4	45.0	4
CAACGGT	20	7.0315733E-4	45.0	41
CGAAACG	40	6.8084773E-9	45.0	1
CGTTGCC	20	7.0315733E-4	45.0	12
CGCATAA	20	7.0315733E-4	45.0	12
ATTCGGT	20	7.0315733E-4	45.0	31
TTCGTAC	20	7.0315733E-4	45.0	33
CAATTCG	20	7.0315733E-4	45.0	44
TTCCGCA	20	7.0315733E-4	45.0	13
TCGATAC	20	7.0315733E-4	45.0	25
ATTCCGC	20	7.0315733E-4	45.0	24
CCCGTTG	20	7.0315733E-4	45.0	32
TACGATT	40	6.8084773E-9	45.0	11
>>END_MODULE