FastQCFastQC Report
Sat 18 Jun 2016
SRR3553041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527915
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT42050.7965297443717265TruSeq Adapter, Index 22 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT35540.6732144379303486No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGT35250.6677211293484747No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC32010.6063476127785723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17310.3278936950077191No Hit
AAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT12060.2284458672324143TruSeq Adapter, Index 23 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT9190.1740810547152477No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT8860.16783004839794285No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC8670.1642309841546461TruSeq Adapter, Index 23 (95% over 23bp)
AACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG8510.16120019321292253TruSeq Adapter, Index 23 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT7970.15097127378460548No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACTC6050.11460178248392261No Hit
AACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT5950.11270753814534536No Hit
CACCCTGGGAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC5510.10437286305560553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA302.1632623E-645.00000421
CGAAACG302.1632623E-645.0000041
GTAATCA302.1632623E-645.00000421
CGTTACG453.8380676E-1045.0000041
AATTGCG302.1632623E-645.0000041
GACGGTA302.1632623E-645.0000044
ACGTCCC302.1632623E-645.00000439
TCGAAAG453.8380676E-1045.0000041
ACGGTAA302.1632623E-645.0000045
AATCCGA207.0298184E-445.031
ACTTGCG253.8879298E-545.01
ACGCAGA207.0298184E-445.02
CGATTGG207.0298184E-445.02
ATCACGT207.0298184E-445.036
CGTTCAT551.8189894E-1245.017
CATTACG207.0298184E-445.01
ATCCGAA207.0298184E-445.032
ATATCCG253.8879298E-545.01
GTTCGCA207.0298184E-445.015
TACGATG207.0298184E-445.01