Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553041_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 527915 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT | 4205 | 0.7965297443717265 | TruSeq Adapter, Index 22 (95% over 22bp) |
AATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT | 3554 | 0.6732144379303486 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGT | 3525 | 0.6677211293484747 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC | 3201 | 0.6063476127785723 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1731 | 0.3278936950077191 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT | 1206 | 0.2284458672324143 | TruSeq Adapter, Index 23 (95% over 21bp) |
AATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT | 919 | 0.1740810547152477 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT | 886 | 0.16783004839794285 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC | 867 | 0.1642309841546461 | TruSeq Adapter, Index 23 (95% over 23bp) |
AACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG | 851 | 0.16120019321292253 | TruSeq Adapter, Index 23 (95% over 22bp) |
AATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT | 797 | 0.15097127378460548 | No Hit |
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACTC | 605 | 0.11460178248392261 | No Hit |
AACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT | 595 | 0.11270753814534536 | No Hit |
CACCCTGGGAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC | 551 | 0.10437286305560553 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTGGTA | 30 | 2.1632623E-6 | 45.000004 | 21 |
CGAAACG | 30 | 2.1632623E-6 | 45.000004 | 1 |
GTAATCA | 30 | 2.1632623E-6 | 45.000004 | 21 |
CGTTACG | 45 | 3.8380676E-10 | 45.000004 | 1 |
AATTGCG | 30 | 2.1632623E-6 | 45.000004 | 1 |
GACGGTA | 30 | 2.1632623E-6 | 45.000004 | 4 |
ACGTCCC | 30 | 2.1632623E-6 | 45.000004 | 39 |
TCGAAAG | 45 | 3.8380676E-10 | 45.000004 | 1 |
ACGGTAA | 30 | 2.1632623E-6 | 45.000004 | 5 |
AATCCGA | 20 | 7.0298184E-4 | 45.0 | 31 |
ACTTGCG | 25 | 3.8879298E-5 | 45.0 | 1 |
ACGCAGA | 20 | 7.0298184E-4 | 45.0 | 2 |
CGATTGG | 20 | 7.0298184E-4 | 45.0 | 2 |
ATCACGT | 20 | 7.0298184E-4 | 45.0 | 36 |
CGTTCAT | 55 | 1.8189894E-12 | 45.0 | 17 |
CATTACG | 20 | 7.0298184E-4 | 45.0 | 1 |
ATCCGAA | 20 | 7.0298184E-4 | 45.0 | 32 |
ATATCCG | 25 | 3.8879298E-5 | 45.0 | 1 |
GTTCGCA | 20 | 7.0298184E-4 | 45.0 | 15 |
TACGATG | 20 | 7.0298184E-4 | 45.0 | 1 |