##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421283
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85931547202237	33.0	31.0	34.0	30.0	34.0
2	32.061194019222235	33.0	31.0	34.0	30.0	34.0
3	32.21047371956618	34.0	31.0	34.0	30.0	34.0
4	35.87025586126191	37.0	35.0	37.0	35.0	37.0
5	35.63021057104132	37.0	35.0	37.0	33.0	37.0
6	35.010456154176644	37.0	35.0	37.0	33.0	37.0
7	35.34251322745043	37.0	35.0	37.0	33.0	37.0
8	35.72810675958916	37.0	35.0	37.0	35.0	37.0
9	37.41386668818822	39.0	37.0	39.0	35.0	39.0
10	37.01314555773672	39.0	37.0	39.0	33.0	39.0
11	37.01223880384445	39.0	37.0	39.0	33.0	39.0
12	36.86922092750004	39.0	37.0	39.0	33.0	39.0
13	36.882214568354286	39.0	37.0	39.0	33.0	39.0
14	38.047262766358955	40.0	37.0	41.0	33.0	41.0
15	38.093037222009904	40.0	37.0	41.0	33.0	41.0
16	37.95555481707071	40.0	37.0	41.0	33.0	41.0
17	37.95792377095682	40.0	37.0	41.0	33.0	41.0
18	37.9282074045238	40.0	37.0	41.0	33.0	41.0
19	37.88979854397163	40.0	37.0	41.0	33.0	41.0
20	37.87004222814593	40.0	37.0	41.0	33.0	41.0
21	37.838400789967785	40.0	37.0	41.0	33.0	41.0
22	37.80296855083163	40.0	36.0	41.0	33.0	41.0
23	37.6658065955664	39.0	36.0	41.0	33.0	41.0
24	37.55559327103159	39.0	36.0	41.0	32.0	41.0
25	37.59643754910594	39.0	36.0	41.0	33.0	41.0
26	37.52023699033666	39.0	36.0	41.0	33.0	41.0
27	37.43056567675411	39.0	36.0	41.0	32.0	41.0
28	37.38865323309984	39.0	36.0	41.0	32.0	41.0
29	37.15618004999015	39.0	35.0	41.0	32.0	41.0
30	37.23045316331302	39.0	36.0	41.0	32.0	41.0
31	37.12317135986973	39.0	35.0	41.0	32.0	41.0
32	37.11044119985853	39.0	35.0	41.0	32.0	41.0
33	36.83975142600105	39.0	35.0	41.0	31.0	41.0
34	36.81943016926864	39.0	35.0	41.0	31.0	41.0
35	36.773119257126446	39.0	35.0	41.0	31.0	41.0
36	36.70569427202142	39.0	35.0	41.0	31.0	41.0
37	36.58957517868036	39.0	35.0	40.0	31.0	41.0
38	36.36554999845709	39.0	35.0	40.0	30.0	41.0
39	36.37575216659585	39.0	35.0	40.0	30.0	41.0
40	36.27539444981165	38.0	35.0	40.0	30.0	41.0
41	36.24229793274355	38.0	35.0	40.0	30.0	41.0
42	36.172444651220204	38.0	35.0	40.0	30.0	41.0
43	36.111025130375545	38.0	35.0	40.0	30.0	41.0
44	35.99810816007292	38.0	35.0	40.0	30.0	41.0
45	35.71994597455867	38.0	35.0	40.0	29.0	41.0
46	35.57064016350054	38.0	34.0	40.0	29.0	41.0
47	35.43087663162292	38.0	34.0	40.0	28.0	41.0
48	35.33665968007254	37.0	34.0	40.0	28.0	41.0
49	35.225945504565814	37.0	34.0	40.0	27.0	41.0
50	35.110716549208014	37.0	34.0	40.0	27.0	41.0
51	33.8092873436621	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	5.0
12	3.0
13	9.0
14	17.0
15	27.0
16	37.0
17	100.0
18	167.0
19	387.0
20	622.0
21	985.0
22	1445.0
23	1771.0
24	2101.0
25	2441.0
26	2963.0
27	3501.0
28	4412.0
29	5508.0
30	7226.0
31	9634.0
32	13046.0
33	17884.0
34	27518.0
35	34268.0
36	41514.0
37	59236.0
38	86781.0
39	97645.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.154385531815905	34.419143426152964	19.40121011291697	26.025260929114157
2	23.016119805451442	32.7079421671418	19.66848887802261	24.60744914938414
3	20.824006665353217	28.98621591661662	22.849248604857067	27.340528813173094
4	22.2069250361396	32.145137591595194	18.50679946734143	27.141137904923767
5	17.712084275890554	35.48161212296722	16.984782201038257	29.82152140010397
6	19.627186475599537	37.495460296285394	20.327665725889723	22.549687502225346
7	82.31189010712514	7.507067695587052	5.804649131344013	4.3763930659437955
8	83.49280649824465	5.097048777187782	4.476563260326194	6.933581464241377
9	77.99958697597576	8.155325517526224	8.688933567222033	5.156153939275974
10	36.717123643726424	31.46364795161447	14.612742503257905	17.206485901401194
11	27.399396605132413	28.401810659343006	23.951832853450057	20.24695988207452
12	27.06850264549009	23.62283785483867	29.125789552391147	20.182869947280093
13	25.677276320193315	25.364897230602708	26.53584407630975	22.42198237289423
14	20.810951308265466	27.060669431237432	29.05386640334407	23.074512857153028
15	20.019321928489873	28.414391276173024	27.444022189359647	24.122264605977456
16	21.525910136416613	27.26908040438375	28.36335669846635	22.841652760733282
17	20.899965106591058	26.70224053664639	27.548227675932807	24.849566680829753
18	22.811981494624753	25.888773105014916	25.794774533983094	25.504470866377233
19	22.6531808784119	29.41182055767738	25.96283258522181	21.97216597868891
20	25.64807979434252	27.194546183919122	25.108062751167264	22.04931127057109
21	24.665367460827994	27.927782511993126	24.620267136343028	22.786582890835852
22	22.97078211083761	27.363553715673312	24.235727527576476	25.429936645912605
23	23.218121785118317	28.38115945813147	23.732977594633535	24.667741162116677
24	21.639135687886764	28.039583842690067	25.814950994936897	24.50632947448627
25	22.882955163156357	27.764234493202906	24.02992762584771	25.32288271779303
26	25.46079476266548	27.512622156602568	24.378386975026288	22.648196105705665
27	22.431951918306698	26.5339451152788	25.459607912021138	25.574495054393363
28	23.055048506585834	27.48152666972083	27.581934234232097	21.88149058946124
29	24.59296957152318	28.26223702356848	23.45834035553298	23.686453049375363
30	25.494738691093637	24.19181405373585	25.93624713078857	24.377200124381947
31	25.714068690167892	25.948590377489715	23.99622106754842	24.341119864793974
32	26.591388686464917	24.739189570906017	24.43772950724335	24.231692235385715
33	25.778633365220056	24.26041402097877	25.42827505501053	24.532677558790645
34	24.43488106569693	24.185405060256407	26.296100246152825	25.083613627893836
35	26.557207387907887	22.879394611223333	28.003503583102095	22.559894417766678
36	26.944358068091994	25.702674923982215	25.63193862557948	21.721028382346308
37	26.14442073380602	25.203248172843434	24.945226842763653	23.707104250586898
38	25.966155767025967	25.28205505562769	26.939135925256895	21.81265325208945
39	25.692942748698616	22.74765418970146	25.07696726428553	26.482435797314395
40	26.39152303795786	23.842405224041798	26.132552227362606	23.633519510637743
41	22.405366463873452	22.073523023715648	29.549732602549827	25.971377909861065
42	25.429461905654872	23.887505548526764	26.490506381695916	24.192526164122455
43	23.851425288938792	23.398997823315916	27.755689168563652	24.993887719181643
44	25.498061872897793	22.04290227709165	25.590161482898672	26.868874367111893
45	26.488370050536105	22.92425756557943	26.912787840952518	23.67458454293195
46	23.19462214236036	22.800825098567945	29.320670428191974	24.683882330879715
47	24.00286743115673	25.119931257610677	26.594711868269073	24.282489442963517
48	23.858546392804836	23.696185224658958	28.297842542898717	24.14742583963749
49	23.869228048603908	23.337518959939043	28.24253530287242	24.550717688584633
50	23.392588829836473	22.689023767871003	27.37898277404975	26.539404628242774
51	23.549727855147253	22.553722794416107	28.628736502541045	25.267812847895595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1790.0
1	1501.5
2	1213.0
3	877.0
4	541.0
5	514.5
6	488.0
7	492.5
8	497.0
9	564.5
10	632.0
11	694.5
12	757.0
13	812.0
14	867.0
15	792.5
16	718.0
17	834.5
18	951.0
19	932.0
20	913.0
21	927.0
22	941.0
23	1357.5
24	1774.0
25	2107.5
26	2591.5
27	2742.0
28	4087.0
29	5432.0
30	5838.0
31	6244.0
32	6030.0
33	5816.0
34	8314.0
35	10812.0
36	11204.5
37	11597.0
38	12141.5
39	12686.0
40	13227.0
41	13768.0
42	14819.5
43	15871.0
44	18287.0
45	20703.0
46	26264.0
47	31825.0
48	33711.0
49	35597.0
50	37362.5
51	39128.0
52	37451.0
53	35774.0
54	32172.5
55	28571.0
56	26585.0
57	24599.0
58	23004.5
59	21410.0
60	19943.5
61	18477.0
62	17715.0
63	16953.0
64	14588.0
65	12223.0
66	11010.0
67	9797.0
68	8346.5
69	6896.0
70	6353.5
71	5811.0
72	5473.0
73	5135.0
74	4378.5
75	3135.0
76	2648.0
77	2014.5
78	1381.0
79	1061.0
80	741.0
81	521.5
82	302.0
83	192.0
84	82.0
85	73.0
86	64.0
87	46.5
88	29.0
89	21.5
90	14.0
91	8.0
92	2.0
93	1.5
94	1.0
95	4.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.931413981746445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40113530994869	18.29994091142517
2	11.079554212879597	5.524579056290082
3	3.839965935908675	2.8720734127183083
4	1.8282432385566876	1.823227561591424
5	1.03756344281057	1.2933961862518213
6	0.7065739358718036	1.0569532382359141
7	0.533158303512962	0.9304673267881094
8	0.391645743023356	0.7811425722803179
9	0.33683506653496903	0.7557997038617179
>10	5.180084201314204	33.08176136914374
>50	1.527716250494214	25.241769016309014
>100	0.12755882587296763	4.860521947596371
>500	0.004982766635662798	0.7140591973312871
>1k	0.004982766635662798	2.7643085001767225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	2641	0.6268945103410296	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGT	2578	0.6119401922223304	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	2301	0.546188666525827	No Hit
AATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	2199	0.5219769133812663	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1407	0.33397977131761786	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	636	0.15096740196020253	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	601	0.14265944744981401	No Hit
AAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	589	0.1398110059033951	No Hit
GCAATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	542	0.1286546098465877	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTTC	506	0.12010928520733094	No Hit
AACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	461	0.10942762940826	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	459	0.10895288915052354	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	450	0.10681655799070933	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	445	0.10562970734636812	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	433	0.1027812657999492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6038696078408101	0.0
2	0.0	0.0	0.0	0.8341186328430058	0.0
3	0.0	0.0	0.0	1.1809163911195086	0.0
4	0.0	0.0	0.0	2.757766157191247	0.0
5	0.0	0.0	0.0	2.987540441935706	0.0
6	0.0	0.0	0.0	4.181037449885231	0.0
7	0.0	0.0	0.0	5.053847413733761	0.0
8	0.0	0.0	0.0	5.7913564041273915	0.0
9	0.0	0.0	0.0	7.1980117878006	0.0
10	0.0	0.0	0.0	8.65166645698972	0.0
11	0.0	0.0	0.0	10.401796417135275	0.0
12	0.0	0.0	0.0	11.037473622244429	0.0
13	0.0	0.0	0.0	11.336322614489548	0.0
14	0.0	0.0	0.0	11.717539041451946	0.0
15	0.0	0.0	0.0	11.982444105268904	0.0
16	0.0	0.0	0.0	12.482820336923162	0.0
17	0.0	0.0	0.0	13.054882347495626	0.0
18	0.0	0.0	0.0	14.026438284953345	0.0
19	0.0	0.0	0.0	14.354958543306994	0.0
20	0.0	0.0	0.0	14.740685002717887	0.0
21	0.0	0.0	0.0	15.104336040144036	0.0
22	0.0	0.0	0.0	15.477244512596046	0.0
23	0.0	0.0	0.0	15.839708699377853	0.0
24	0.0	0.0	0.0	16.137845581236366	0.0
25	0.0	0.0	0.0	16.398003242475962	0.0
26	0.0	0.0	0.0	16.653650871267057	0.0
27	0.0	0.0	0.0	16.921404376630438	0.0
28	0.0	0.0	0.0	17.179900446967952	0.0
29	0.0	0.0	0.0	17.43982073807868	0.0
30	0.0	0.0	0.0	17.880617067387007	0.0
31	2.3737012886824297E-4	0.0	0.0	18.134128365018288	0.0
32	2.3737012886824297E-4	0.0	0.0	18.434638948165485	0.0
33	2.3737012886824297E-4	0.0	0.0	18.730639498864186	0.0
34	2.3737012886824297E-4	0.0	0.0	18.97204491992319	0.0
35	2.3737012886824297E-4	0.0	0.0	19.26211121740018	0.0
36	2.3737012886824297E-4	0.0	0.0	19.497345015108607	0.0
37	2.3737012886824297E-4	0.0	0.0	19.74990683222442	0.0
38	2.3737012886824297E-4	0.0	0.0	20.026917772613658	0.0
39	2.3737012886824297E-4	0.0	0.0	20.302504492229687	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTCG	20	7.0281915E-4	45.000004	29
ATAGCGA	20	7.0281915E-4	45.000004	12
ATTCGGC	20	7.0281915E-4	45.000004	17
AAGCGCG	20	7.0281915E-4	45.000004	1
ATCCGTT	20	7.0281915E-4	45.000004	28
TCTACGT	20	7.0281915E-4	45.000004	28
TCTACCG	20	7.0281915E-4	45.000004	25
GTATGCG	20	7.0281915E-4	45.000004	1
TACGAAG	20	7.0281915E-4	45.000004	1
TGCGACG	20	7.0281915E-4	45.000004	1
TATTCCG	20	7.0281915E-4	45.000004	1
GTCGTTT	20	7.0281915E-4	45.000004	23
TCGTCGG	20	7.0281915E-4	45.000004	13
AATCTGA	20	7.0281915E-4	45.000004	35
TGTTACG	20	7.0281915E-4	45.000004	1
GGACGTA	20	7.0281915E-4	45.000004	9
GCGATTG	20	7.0281915E-4	45.000004	1
CATAACG	20	7.0281915E-4	45.000004	1
CAGTACG	20	7.0281915E-4	45.000004	1
ATCGGAC	20	7.0281915E-4	45.000004	40
>>END_MODULE