FastQCFastQC Report
Sat 18 Jun 2016
SRR3553039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430052
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC30860.7175876405643968No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGT29490.6857310278756987No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT26120.6073684112618939No Hit
AATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT24740.5752792685535703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15210.3536781598504367No Hit
AATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT6570.1527722228939756No Hit
AAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT6380.1483541525210905No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6230.14486620222670749No Hit
AACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG5790.134634881363184No Hit
GCAATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5700.13254211118655418No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTTC5320.12370597044078391No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT5300.12324091040153283No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTAT4780.11114934938100508No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC4720.10975416926325189No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA4510.10487103885111568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCC351.2095552E-745.00000443
CGCATTA351.2095552E-745.00000417
ATTCGAT351.2095552E-745.00000427
TATGCGG351.2095552E-745.0000042
TATTCGA351.2095552E-745.00000426
ATGAACG453.8380676E-1045.0000041
CACGTTC351.2095552E-745.00000442
TCACGCC207.0283574E-445.043
ACGCCTA207.0283574E-445.012
CATATAG207.0283574E-445.01
GGTGTCG207.0283574E-445.07
TACTTCG253.8867205E-545.029
CCGATTC253.8867205E-545.010
CGACGAA207.0283574E-445.033
CTATCGG207.0283574E-445.02
CCCTACG207.0283574E-445.031
TAGAACG253.8867205E-545.01
CAACCGA207.0283574E-445.025
CGACACG253.8867205E-545.01
CGTTATG253.8867205E-545.01