##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553039_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430052
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.853547943039448	33.0	31.0	34.0	30.0	34.0
2	32.05990670895613	33.0	31.0	34.0	30.0	34.0
3	32.18769358124134	34.0	31.0	34.0	30.0	34.0
4	35.848560174118475	37.0	35.0	37.0	35.0	37.0
5	35.61968320110126	37.0	35.0	37.0	33.0	37.0
6	35.64223861300494	37.0	35.0	37.0	33.0	37.0
7	35.65082594662971	37.0	35.0	37.0	35.0	37.0
8	35.70053388892506	37.0	35.0	37.0	35.0	37.0
9	37.43158966822617	39.0	37.0	39.0	35.0	39.0
10	37.01134048905714	39.0	37.0	39.0	33.0	39.0
11	37.012721717373715	39.0	37.0	39.0	33.0	39.0
12	36.90879940100267	39.0	37.0	39.0	33.0	39.0
13	36.90471617385805	39.0	37.0	39.0	33.0	39.0
14	38.06089961213993	40.0	37.0	41.0	33.0	41.0
15	38.10561513491392	40.0	37.0	41.0	33.0	41.0
16	37.97403569800861	40.0	37.0	41.0	33.0	41.0
17	37.95064550333448	40.0	37.0	41.0	33.0	41.0
18	37.88389311060058	40.0	37.0	41.0	33.0	41.0
19	37.866483588031215	40.0	37.0	41.0	33.0	41.0
20	37.85743351966739	40.0	37.0	41.0	33.0	41.0
21	37.80454921730395	40.0	36.0	41.0	33.0	41.0
22	37.76312166900747	40.0	36.0	41.0	33.0	41.0
23	37.590503008938455	39.0	36.0	41.0	33.0	41.0
24	37.49127547366365	39.0	36.0	41.0	32.0	41.0
25	37.523332062169224	39.0	36.0	41.0	32.0	41.0
26	37.44562750551096	39.0	36.0	41.0	32.0	41.0
27	37.396668309878805	39.0	36.0	41.0	32.0	41.0
28	37.326679099271715	39.0	36.0	41.0	32.0	41.0
29	37.16323142317673	39.0	35.0	41.0	31.0	41.0
30	37.2148530875336	39.0	36.0	41.0	32.0	41.0
31	37.08879158799401	39.0	35.0	41.0	31.0	41.0
32	37.05992996195809	39.0	35.0	41.0	31.0	41.0
33	36.94928520271967	39.0	35.0	41.0	31.0	41.0
34	36.892485094825744	39.0	35.0	41.0	31.0	41.0
35	36.81909397003153	39.0	35.0	41.0	31.0	41.0
36	36.695822830727444	39.0	35.0	41.0	31.0	41.0
37	36.57237264330825	39.0	35.0	40.0	30.0	41.0
38	36.46058616167347	39.0	35.0	40.0	30.0	41.0
39	36.39084575818738	39.0	35.0	40.0	30.0	41.0
40	36.26369834345614	38.0	35.0	40.0	30.0	41.0
41	36.19675062550575	38.0	35.0	40.0	30.0	41.0
42	36.11911815315357	38.0	35.0	40.0	30.0	41.0
43	36.04674085924493	38.0	35.0	40.0	30.0	41.0
44	35.95513565801345	38.0	35.0	40.0	30.0	41.0
45	35.69573679462019	38.0	35.0	40.0	29.0	41.0
46	35.54689897965827	38.0	34.0	40.0	29.0	41.0
47	35.365334889734264	37.0	34.0	40.0	28.0	41.0
48	35.296219992000964	37.0	34.0	40.0	28.0	41.0
49	35.120478453768385	37.0	34.0	40.0	27.0	41.0
50	35.010894031419454	37.0	34.0	40.0	27.0	41.0
51	33.62764270367305	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	5.0
11	2.0
12	6.0
13	8.0
14	11.0
15	28.0
16	46.0
17	90.0
18	186.0
19	358.0
20	618.0
21	900.0
22	1376.0
23	1725.0
24	2147.0
25	2563.0
26	3103.0
27	3759.0
28	4538.0
29	5779.0
30	7595.0
31	9872.0
32	13126.0
33	18401.0
34	28221.0
35	34906.0
36	42262.0
37	59699.0
38	88930.0
39	99761.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.17849004306456	34.60279222047566	19.31510608019495	25.903611656264825
2	22.724461227944527	32.89020862593361	19.64948424841647	24.735845897705396
3	20.858175290429994	28.79628510040646	23.059769516244547	27.285770092918998
4	22.3198589937961	31.853357268423355	18.62123650163236	27.205547236148185
5	17.676467031893818	35.41618222912578	17.248379265763212	29.658971473217193
6	20.096639476156373	36.19585538493019	20.834922288467443	22.872582850445994
7	81.78848139294783	7.632797894208141	5.9739287341995855	4.604791978644443
8	82.73836652311813	5.144959214234557	4.769888292578572	7.346785970068735
9	77.55108684531173	8.066466380809763	9.004027419939915	5.378419353938593
10	36.81926836754625	31.177625031391553	14.756587575455992	17.246519025606204
11	27.278329132290978	28.217982941597757	24.201259382586294	20.302428543524968
12	26.746067917368133	23.521341605201233	29.411559532335623	20.32103094509501
13	25.509938333038797	25.25880591184322	26.623059536986226	22.60819621813176
14	20.763303042422777	26.922325672244284	29.365053528410517	22.94931775692242
15	19.802721531349697	28.316808199938613	27.49179169030722	24.38867857840447
16	21.298587147600756	27.273213471859215	28.500739445462408	22.927459935077618
17	20.694009096574366	26.670960721029086	27.84198189986327	24.793048282533274
18	22.723066047826773	25.740608112507324	25.886404434812533	25.649921404853366
19	22.39333847999777	29.399235441295474	26.281007878117062	21.926418200589694
20	25.59132383990773	27.31483634537219	25.030228902551322	22.06361091216876
21	24.45169421372299	27.906857775338796	24.744682038451163	22.89676597248705
22	22.768409401653752	27.612939830532124	24.12173411587436	25.496916651939767
23	23.268581473868277	28.391217806218783	23.637141554974747	24.703059164938193
24	21.468334061927393	28.139387794964332	25.821063499297757	24.571214643810517
25	22.67795522401942	27.711765088872976	24.173588310250853	25.43669137685675
26	25.408090184442813	27.514114572191268	24.32589547310558	22.75189977026034
27	22.494489038534876	26.360533144828995	25.655269595304752	25.489708221331377
28	23.01419363239794	27.307627914763792	27.76361928324947	21.91455916958879
29	24.689572423799913	28.098229981490608	23.541106656869403	23.671090937840074
30	25.666198506227154	23.905481197622613	25.940816459404907	24.487503836745326
31	25.67549970701217	25.68945150818971	24.22893045492173	24.406118329876385
32	26.60375954535731	24.57144717383014	24.44402072307535	24.380772557737203
33	25.955930910680568	24.130802786639755	25.58202263912271	24.331243663556965
34	24.453786983899622	23.957335391999106	26.2721717373713	25.316705886729977
35	26.635151098006755	22.5921516467776	28.26960460595463	22.50309264926102
36	27.135788230260527	25.454131128328665	25.77246472519602	21.637615916214784
37	26.325421111865545	25.006975900588767	24.952563875996393	23.715039111549302
38	26.164510338284675	25.092081887771712	27.14485690102592	21.598550872917695
39	25.841293611005177	22.472166156650825	25.331355277966384	26.35518495437761
40	26.461683703366106	23.70736562090166	26.18799587026685	23.642954805465386
41	22.479607117278842	21.766902607126582	29.663854603629332	26.08963567196525
42	25.39995163375592	23.80456316910513	26.560508961706958	24.234976235431994
43	23.840372792127464	23.303460976812108	27.949178238910644	24.906987992149787
44	25.368792611126096	21.90897844911778	25.707588849720498	27.014640090035623
45	26.620501706770344	22.853747918856325	26.973482276561906	23.552268097811428
46	23.20277547831425	22.51030107986941	29.573167895975374	24.713755545840968
47	23.959893222214987	24.983722898626212	26.49842344646694	24.55796043269186
48	23.828746291146185	23.518318714946098	28.49562378503065	24.157311208877065
49	24.152195548445306	22.98884786025876	28.243793773776193	24.615162817519742
50	23.299042906439222	22.476119166984457	27.418079674085927	26.806758252490397
51	23.577846399970237	22.360319217210943	28.72885139471506	25.332982988103765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1916.0
1	1562.5
2	1209.0
3	875.5
4	542.0
5	494.0
6	446.0
7	483.0
8	520.0
9	596.5
10	673.0
11	713.0
12	753.0
13	814.5
14	876.0
15	817.0
16	758.0
17	870.0
18	982.0
19	981.5
20	981.0
21	982.0
22	983.0
23	1317.5
24	1652.0
25	2047.0
26	2623.0
27	2804.0
28	4093.5
29	5383.0
30	5763.0
31	6143.0
32	6001.5
33	5860.0
34	8437.0
35	11014.0
36	11207.0
37	11400.0
38	12208.5
39	13017.0
40	13451.5
41	13886.0
42	14939.0
43	15992.0
44	18394.0
45	20796.0
46	26804.5
47	32813.0
48	35375.0
49	37937.0
50	39178.5
51	40420.0
52	38469.5
53	36519.0
54	32812.0
55	29105.0
56	27201.5
57	25298.0
58	23520.0
59	21742.0
60	20251.0
61	18760.0
62	18012.0
63	17264.0
64	14891.0
65	12518.0
66	11105.0
67	9692.0
68	8296.5
69	6901.0
70	6387.5
71	5874.0
72	5503.5
73	5133.0
74	4437.0
75	3175.0
76	2609.0
77	2013.0
78	1417.0
79	1067.5
80	718.0
81	528.0
82	338.0
83	218.0
84	98.0
85	77.0
86	56.0
87	43.0
88	30.0
89	26.5
90	23.0
91	12.5
92	2.0
93	3.5
94	5.0
95	6.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.32133172897847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78804693138144	20.979517030326395
2	9.565998089823566	5.227116142616881
3	3.3612643986540967	2.7550265899330166
4	1.64295900990554	1.7955131250697314
5	0.9639810198054939	1.3168622611272431
6	0.6525205470542937	1.069663819562692
7	0.434429769134669	0.8308439884830275
8	0.336937654973128	0.7364468354803938
9	0.30187528999132335	0.7422871444771068
>10	4.471687005538635	31.323715542196567
>50	1.3639970840911777	24.918599093502383
>100	0.10604115261900066	4.385965028159123
>500	0.005986194099459714	0.9647160452412645
>1k	0.0042758529281855095	2.9537273538241866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	3086	0.7175876405643968	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGT	2949	0.6857310278756987	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	2612	0.6073684112618939	No Hit
AATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	2474	0.5752792685535703	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1521	0.3536781598504367	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	657	0.1527722228939756	No Hit
AAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	638	0.1483541525210905	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	623	0.14486620222670749	No Hit
AACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	579	0.134634881363184	No Hit
GCAATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	570	0.13254211118655418	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTTC	532	0.12370597044078391	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	530	0.12324091040153283	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTAT	478	0.11114934938100508	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	472	0.10975416926325189	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	451	0.10487103885111568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6966599387980988	0.0
2	0.0	0.0	0.0	0.9263995981881261	0.0
3	0.0	0.0	0.0	1.3198403913945291	0.0
4	0.0	0.0	0.0	3.0342842260935887	0.0
5	0.0	0.0	0.0	3.291462427799429	0.0
6	0.0	0.0	0.0	4.564099225209975	0.0
7	0.0	0.0	0.0	5.496312073888739	0.0
8	0.0	0.0	0.0	6.296215341400575	0.0
9	0.0	0.0	0.0	7.834168891203855	0.0
10	0.0	0.0	0.0	9.325848967101653	0.0
11	0.0	0.0	0.0	11.20538911573484	0.0
12	0.0	0.0	0.0	11.910652665259084	0.0
13	0.0	0.0	0.0	12.225265781812432	0.0
14	0.0	0.0	0.0	12.619171635058086	0.0
15	0.0	0.0	0.0	12.879372727019058	0.0
16	0.0	0.0	0.0	13.389311060057853	0.0
17	0.0	0.0	0.0	13.993889111084242	0.0
18	0.0	0.0	0.0	14.988652535042274	0.0
19	0.0	0.0	0.0	15.323495763303043	0.0
20	0.0	0.0	0.0	15.709263065861803	0.0
21	0.0	0.0	0.0	16.09200747816543	0.0
22	0.0	0.0	0.0	16.48940128170547	0.0
23	0.0	0.0	0.0	16.8958637560109	0.0
24	0.0	0.0	0.0	17.20931422246612	0.0
25	0.0	0.0	0.0	17.467887604289714	0.0
26	0.0	0.0	0.0	17.7127417149554	0.0
27	0.0	0.0	0.0	17.998753639094808	0.0
28	0.0	0.0	0.0	18.27244147219406	0.0
29	0.0	0.0	0.0	18.563801586784855	0.0
30	0.0	0.0	0.0	19.02421102564341	0.0
31	0.0	0.0	0.0	19.27301814664273	0.0
32	0.0	0.0	0.0	19.580190302568063	0.0
33	0.0	0.0	0.0	19.86596969668784	0.0
34	0.0	0.0	0.0	20.11547440774604	0.0
35	0.0	0.0	0.0	20.392185131100426	0.0
36	0.0	0.0	0.0	20.657269353473534	0.0
37	0.0	0.0	0.0	20.91933068559151	0.0
38	0.0	0.0	0.0	21.201157069377658	0.0
39	0.0	0.0	0.0	21.46600876173114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCC	35	1.2095552E-7	45.000004	43
CGCATTA	35	1.2095552E-7	45.000004	17
ATTCGAT	35	1.2095552E-7	45.000004	27
TATGCGG	35	1.2095552E-7	45.000004	2
TATTCGA	35	1.2095552E-7	45.000004	26
ATGAACG	45	3.8380676E-10	45.000004	1
CACGTTC	35	1.2095552E-7	45.000004	42
TCACGCC	20	7.0283574E-4	45.0	43
ACGCCTA	20	7.0283574E-4	45.0	12
CATATAG	20	7.0283574E-4	45.0	1
GGTGTCG	20	7.0283574E-4	45.0	7
TACTTCG	25	3.8867205E-5	45.0	29
CCGATTC	25	3.8867205E-5	45.0	10
CGACGAA	20	7.0283574E-4	45.0	33
CTATCGG	20	7.0283574E-4	45.0	2
CCCTACG	20	7.0283574E-4	45.0	31
TAGAACG	25	3.8867205E-5	45.0	1
CAACCGA	20	7.0283574E-4	45.0	25
CGACACG	25	3.8867205E-5	45.0	1
CGTTATG	25	3.8867205E-5	45.0	1
>>END_MODULE