SRR3553038_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248631
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3101	1.2472298305521035	Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1919	0.7718265220346618	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	1717	0.6905816249783816	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	1711	0.6881684102143337	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1658	0.666851679798577	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	838	0.3370456620453604	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	690	0.27751969786551156	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	571	0.22965760504522767	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.19185057374181014	No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	413	0.16610961625863227	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	404	0.16248979411256037	No Hit
ATGCTTGGGATTATTTGTGTGTGTGTGTATGTGTGTATGTGTGTGTGTGTG	389	0.15645675720244057	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	385	0.15484794735974194	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	354	0.14237967107882765	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	347	0.1395642538541051	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	324	0.13031359725858804	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	308	0.12387835788779357	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	308	0.12387835788779357	TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTATC	297	0.11945413082037236	No Hit
TACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	291	0.11704091605632444	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTAT	285	0.11462770129227651	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	257	0.10336603239338618	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTTT	30	2.1586166E-6	45.000004	21
TCGTTAG	30	2.1586166E-6	45.000004	1
CAACCCG	30	2.1586166E-6	45.000004	1
TCTAGTT	30	2.1586166E-6	45.000004	36
ACGGGCC	30	2.1586166E-6	45.000004	5
TGCATAG	30	2.1586166E-6	45.000004	1
GTTGTAT	30	2.1586166E-6	45.000004	21
ACTGTTA	30	2.1586166E-6	45.000004	22
CGTACGG	30	2.1586166E-6	45.000004	2
ATGCGGG	95	0.0	45.000004	3
TTTGCGC	30	2.1586166E-6	45.000004	13
CTCTAGT	30	2.1586166E-6	45.000004	35
TCTGCAG	20	7.0226105E-4	45.0	1
CTTCGCT	20	7.0226105E-4	45.0	22
AACGTAC	20	7.0226105E-4	45.0	15
CGGGTAG	20	7.0226105E-4	45.0	6
AGCCGTT	25	3.881959E-5	45.0	45
CAGCGAT	20	7.0226105E-4	45.0	34
TCGTTGT	25	3.881959E-5	45.0	12
ATCTCGC	25	3.881959E-5	45.0	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content