##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248631
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46682433003125	31.0	31.0	34.0	30.0	34.0
2	31.67762668372005	31.0	31.0	34.0	30.0	34.0
3	31.867518531478375	33.0	31.0	34.0	30.0	34.0
4	35.57844757894229	37.0	35.0	37.0	33.0	37.0
5	35.309679806621055	37.0	35.0	37.0	33.0	37.0
6	34.706734075799076	37.0	35.0	37.0	32.0	37.0
7	35.101523945123496	37.0	35.0	37.0	32.0	37.0
8	35.501309169009495	37.0	35.0	37.0	33.0	37.0
9	37.13516013690972	39.0	37.0	39.0	34.0	39.0
10	36.691474514441076	38.0	35.0	39.0	32.0	39.0
11	36.75293507245677	39.0	37.0	39.0	32.0	39.0
12	36.796755030547274	39.0	37.0	39.0	32.0	39.0
13	36.728151356830004	39.0	35.0	39.0	32.0	39.0
14	37.824161106217645	39.0	37.0	41.0	33.0	41.0
15	37.863552815216124	39.0	37.0	41.0	33.0	41.0
16	37.730287051896184	39.0	37.0	41.0	33.0	41.0
17	37.7174205951792	39.0	37.0	41.0	33.0	41.0
18	37.617778957571666	39.0	36.0	41.0	33.0	41.0
19	37.567809323857446	39.0	36.0	41.0	32.0	41.0
20	37.530883115942906	39.0	36.0	41.0	32.0	41.0
21	37.50652975694905	39.0	36.0	41.0	32.0	41.0
22	37.51019784339041	39.0	36.0	41.0	32.0	41.0
23	37.35901798247202	39.0	36.0	40.0	32.0	41.0
24	37.21683136857431	39.0	36.0	40.0	32.0	41.0
25	37.27257662962382	39.0	36.0	41.0	32.0	41.0
26	37.14655855464524	39.0	36.0	40.0	32.0	41.0
27	37.076418467528185	39.0	36.0	40.0	31.0	41.0
28	37.02227397227216	39.0	36.0	40.0	31.0	41.0
29	36.700697016864346	39.0	35.0	40.0	30.0	41.0
30	36.81121018698392	39.0	35.0	40.0	31.0	41.0
31	36.616938354428854	39.0	35.0	40.0	30.0	41.0
32	36.54428852395719	39.0	35.0	40.0	30.0	41.0
33	36.299572458784304	39.0	35.0	40.0	30.0	41.0
34	36.13072384376848	39.0	35.0	40.0	29.0	41.0
35	36.11307117776946	39.0	35.0	40.0	29.0	41.0
36	36.02257160209306	38.0	35.0	40.0	29.0	41.0
37	35.94030913281127	38.0	35.0	40.0	28.0	41.0
38	35.83115540700878	38.0	35.0	40.0	28.0	41.0
39	35.681282704087586	38.0	35.0	40.0	27.0	41.0
40	35.527404064658064	38.0	34.0	40.0	26.0	41.0
41	35.43997731578122	38.0	34.0	40.0	26.0	41.0
42	35.374434402789674	38.0	34.0	40.0	26.0	41.0
43	35.348983031078184	38.0	34.0	40.0	26.0	41.0
44	35.26664414332887	38.0	34.0	40.0	26.0	41.0
45	34.98204568215548	38.0	34.0	40.0	26.0	41.0
46	34.645394178521585	37.0	33.0	40.0	25.0	41.0
47	34.4790150866143	37.0	33.0	40.0	24.0	41.0
48	34.215025479525885	37.0	33.0	40.0	24.0	41.0
49	34.0260506533779	36.0	33.0	39.0	24.0	41.0
50	33.8932273127647	36.0	33.0	39.0	24.0	41.0
51	32.508729804408944	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	0.0
11	2.0
12	3.0
13	5.0
14	0.0
15	8.0
16	29.0
17	64.0
18	163.0
19	321.0
20	543.0
21	830.0
22	1214.0
23	1579.0
24	1786.0
25	2051.0
26	2340.0
27	2816.0
28	3388.0
29	4235.0
30	5490.0
31	7155.0
32	9001.0
33	11753.0
34	16199.0
35	21887.0
36	26050.0
37	35827.0
38	48173.0
39	45701.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.026589604675202	35.97821671472986	19.647188001496193	25.348005679098744
2	19.187068386484388	35.178638222908646	21.27128153770045	24.363011852906517
3	19.642763774428772	32.260257168253354	23.4777642369616	24.619214820356273
4	20.177693047126063	33.178887588434264	18.989586978293133	27.653832386146536
5	16.77184261013309	36.26418266426954	19.353580205203695	27.610394520393676
6	19.143630520731524	37.17396463031561	21.652971672880696	22.02943317607217
7	76.61635113883627	9.226524447876571	8.192461921482035	5.964662491805125
8	78.87914218259188	6.536594390884483	5.800563887849866	8.783699538673778
9	71.21839191412172	11.363426121441012	11.976784873969859	5.441397090467399
10	26.054273200043436	42.960451432041864	14.096391841725286	16.888883526189414
11	17.45800000804405	28.56803857926003	35.054759865020856	18.91920154767507
12	21.867747786880958	23.165655127478068	36.3442209539438	18.622376131697173
13	23.789873346445134	27.003873209696298	29.006841463856077	20.199411980002495
14	16.577176619166558	30.96717625718434	32.23853823537692	20.21710888827218
15	15.109942042625416	32.311336880759036	30.76808603915039	21.810635037465158
16	17.20742787504374	31.907927812702358	27.937787323382846	22.94685698887106
17	15.793284023311655	30.63817464435247	29.906166165924603	23.662375166411266
18	18.294178923786657	28.621933708990433	28.135670934034774	24.94821643318814
19	18.489247117213864	33.58269885895162	29.35474659233965	18.573307431494865
20	18.57531844379824	31.652529250173956	29.232879246755232	20.539273059272574
21	18.459484135123937	32.771054293310165	26.670045167336333	22.09941640422956
22	17.538440500178982	31.075770921566498	27.36263780461809	24.023150773636434
23	18.965857033113327	30.13743258081253	24.776878184940735	26.11983220113341
24	15.276051658884048	33.896014575817176	26.548982226673264	24.278951538625513
25	19.782328028282876	30.441095438621897	25.20401719817722	24.57255933491801
26	20.443146671171334	33.599189159839284	23.84577948847891	22.111884680510475
27	18.37823923806766	29.56268526450845	25.203614995716546	26.855460501707352
28	24.534752303614592	28.59579054904658	25.789221778458842	21.080235368879986
29	20.641834686744616	33.441927997715496	23.676854454995556	22.23938286054434
30	26.599659736718266	29.05550796159771	22.189107552960007	22.155724748724012
31	25.042331808986006	32.73002964232135	20.60362545298052	21.62401309571212
32	24.03240143023195	29.374052310452036	20.325703552654335	26.267842706661682
33	27.612807735157723	27.847291769731047	22.956912050387924	21.582988444723306
34	21.96789619958895	27.850107186955768	25.31140525517735	24.87059135827793
35	26.26703830174033	30.897997433948298	21.02513363176756	21.80983063254381
36	26.023705813032166	28.237428156585466	27.834823493450134	17.90404253693224
37	25.04514722621073	30.330489761936363	21.83838700725171	22.785976004601196
38	26.54616680944854	27.943015955371614	25.322264721615568	20.18855251356428
39	23.748848695456317	25.47389504928991	26.750485659471263	24.026770595782505
40	25.271185009109885	28.787238920327713	24.807847774412682	21.133728296149716
41	21.35091762491403	25.866846853369047	30.730681210307644	22.051554311409276
42	25.29732816905374	28.771150821900726	24.33646649050199	21.595054518543545
43	21.33120970434097	26.47256375914508	26.902518189606283	25.293708346907668
44	25.160579332424355	23.553780502029113	25.307383230570608	25.97825693497593
45	27.37229066367428	24.865764928749833	25.830246429447655	21.93169797812823
46	20.475322868025305	25.524974761795594	31.046410141937248	22.953292228241853
47	23.336188970804123	25.040722999143313	26.398960708841617	25.224127321210947
48	21.482035627093968	24.049293933580284	33.11936162425442	21.34930881507133
49	23.20305995632081	23.406172198961514	28.36774175384405	25.023026090873625
50	21.22301724241949	22.38779556853329	26.715091842931894	29.674095346115326
51	20.85741520566623	21.327187679734223	33.278231596220905	24.537165518378643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2251.0
1	1809.5
2	1368.0
3	923.5
4	479.0
5	477.0
6	475.0
7	534.5
8	594.0
9	680.0
10	766.0
11	891.5
12	1017.0
13	1044.0
14	1071.0
15	1087.0
16	1103.0
17	1139.0
18	1175.0
19	1155.5
20	1136.0
21	1150.5
22	1165.0
23	1335.0
24	1505.0
25	1483.0
26	1842.5
27	2224.0
28	2265.5
29	2307.0
30	3061.0
31	3815.0
32	4710.5
33	5606.0
34	5894.0
35	6182.0
36	6937.5
37	7693.0
38	7771.0
39	7849.0
40	8964.0
41	10079.0
42	9886.0
43	9693.0
44	11932.5
45	14172.0
46	18811.0
47	23450.0
48	25654.5
49	27859.0
50	30362.5
51	32866.0
52	29227.0
53	25588.0
54	21438.0
55	17288.0
56	14585.5
57	11883.0
58	10051.0
59	8219.0
60	6865.5
61	5512.0
62	4854.5
63	4197.0
64	3178.5
65	2160.0
66	1773.0
67	1386.0
68	1225.5
69	1065.0
70	888.0
71	711.0
72	543.0
73	375.0
74	375.0
75	249.0
76	123.0
77	183.5
78	244.0
79	179.0
80	114.0
81	66.0
82	18.0
83	11.0
84	4.0
85	3.5
86	3.0
87	2.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	248631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.81535287232887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33635902058919	30.01838065245283
2	10.273866144424757	7.9756747951783975
3	3.7023221113494356	4.31120817597162
4	1.8806925922471944	2.919989864497991
5	1.1014745044400924	2.137706078485788
6	0.6994311293481302	1.62891996573235
7	0.547110572290093	1.4865402946535227
8	0.4103329292175697	1.274177395417305
9	0.3139668625073829	1.0968061102597826
>10	3.2619395484265388	27.20738765479767
>50	0.39271762670065385	10.08080247434954
>100	0.07149740433336442	4.9535255056690435
>500	0.003108582797102801	0.8442229649560996
>1k	0.005180971328504668	4.0646580675780575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3101	1.2472298305521035	Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1919	0.7718265220346618	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	1717	0.6905816249783816	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	1711	0.6881684102143337	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1658	0.666851679798577	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	838	0.3370456620453604	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	690	0.27751969786551156	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	571	0.22965760504522767	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.19185057374181014	No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	413	0.16610961625863227	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	404	0.16248979411256037	No Hit
ATGCTTGGGATTATTTGTGTGTGTGTGTATGTGTGTATGTGTGTGTGTGTG	389	0.15645675720244057	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	385	0.15484794735974194	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	354	0.14237967107882765	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	347	0.1395642538541051	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	324	0.13031359725858804	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	308	0.12387835788779357	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	308	0.12387835788779357	TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTATC	297	0.11945413082037236	No Hit
TACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	291	0.11704091605632444	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTAT	285	0.11462770129227651	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	257	0.10336603239338618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.022024606746544E-4	0.0	0.0	1.4077086123612905	0.0
2	4.022024606746544E-4	0.0	0.0	2.023882782114861	0.0
3	4.022024606746544E-4	0.0	0.0	2.7949048992281735	0.0
4	4.022024606746544E-4	0.0	0.0	6.230116115850397	0.0
5	4.022024606746544E-4	0.0	0.0	6.735282406457762	0.0
6	4.022024606746544E-4	0.0	0.0	9.126376035168583	0.0
7	4.022024606746544E-4	0.0	0.0	10.937493715586552	0.0
8	4.022024606746544E-4	0.0	0.0	12.471493900599684	0.0
9	4.022024606746544E-4	0.0	0.0	15.063286557187157	0.0
10	4.022024606746544E-4	0.0	0.0	18.14496181087636	0.0
11	4.022024606746544E-4	0.0	0.0	21.255193439273462	0.0
12	4.022024606746544E-4	0.0	0.0	22.645205143365068	0.0
13	4.022024606746544E-4	0.0	0.0	23.240464785163557	0.0
14	4.022024606746544E-4	0.0	0.0	23.894445986220543	0.0
15	4.022024606746544E-4	0.0	0.0	24.398003466985212	0.0
16	4.022024606746544E-4	0.0	0.0	25.362082765222357	0.0
17	4.022024606746544E-4	0.0	0.0	26.601268546560966	0.0
18	4.022024606746544E-4	0.0	0.0	28.514947854450973	0.0
19	4.022024606746544E-4	0.0	0.0	29.17053786535066	0.0
20	4.022024606746544E-4	0.0	0.0	29.98338903837414	0.0
21	4.022024606746544E-4	0.0	0.0	30.71459311188066	0.0
22	4.022024606746544E-4	0.0	0.0	31.52181345045469	0.0
23	4.022024606746544E-4	0.0	0.0	32.254224131343236	0.0
24	4.022024606746544E-4	0.0	0.0	32.770652090849495	0.0
25	4.022024606746544E-4	0.0	0.0	33.18612723272641	0.0
26	4.022024606746544E-4	0.0	0.0	33.60562439921007	0.0
27	4.022024606746544E-4	0.0	0.0	34.13411843253657	0.0
28	4.022024606746544E-4	0.0	0.0	34.62279442225628	0.0
29	4.022024606746544E-4	0.0	0.0	35.12434089071757	0.0
30	4.022024606746544E-4	0.0	0.0	35.89737402013426	0.0
31	4.022024606746544E-4	0.0	0.0	36.36996191142698	0.0
32	4.022024606746544E-4	0.0	0.0	36.801927354191555	0.0
33	4.022024606746544E-4	0.0	0.0	37.25199190768649	0.0
34	4.022024606746544E-4	0.0	0.0	37.658216392967894	0.0
35	4.022024606746544E-4	0.0	0.0	38.11914041290105	0.0
36	4.022024606746544E-4	0.0	0.0	38.56880276393531	0.0
37	4.022024606746544E-4	0.0	0.0	38.99151755010437	0.0
38	4.022024606746544E-4	0.0	0.0	39.38044732957676	0.0
39	4.022024606746544E-4	0.0	0.0	39.80637973543122	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTTT	30	2.1586166E-6	45.000004	21
TCGTTAG	30	2.1586166E-6	45.000004	1
CAACCCG	30	2.1586166E-6	45.000004	1
TCTAGTT	30	2.1586166E-6	45.000004	36
ACGGGCC	30	2.1586166E-6	45.000004	5
TGCATAG	30	2.1586166E-6	45.000004	1
GTTGTAT	30	2.1586166E-6	45.000004	21
ACTGTTA	30	2.1586166E-6	45.000004	22
CGTACGG	30	2.1586166E-6	45.000004	2
ATGCGGG	95	0.0	45.000004	3
TTTGCGC	30	2.1586166E-6	45.000004	13
CTCTAGT	30	2.1586166E-6	45.000004	35
TCTGCAG	20	7.0226105E-4	45.0	1
CTTCGCT	20	7.0226105E-4	45.0	22
AACGTAC	20	7.0226105E-4	45.0	15
CGGGTAG	20	7.0226105E-4	45.0	6
AGCCGTT	25	3.881959E-5	45.0	45
CAGCGAT	20	7.0226105E-4	45.0	34
TCGTTGT	25	3.881959E-5	45.0	12
ATCTCGC	25	3.881959E-5	45.0	41
>>END_MODULE