SRR3553037_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254118
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3140	1.2356464319725482	Illumina PCR Primer Index 8 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	2004	0.7886100158194225	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	2002	0.787822979875491	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1875	0.7378461974358369	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1831	0.7205314066693426	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	891	0.35062451302150965	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	718	0.2825459038714298	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	692	0.27231443660031956	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	508	0.19990712975861608	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	472	0.185740482767848	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	412	0.16212940444990123	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	379	0.14914331137503048	No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	379	0.14914331137503048	TruSeq Adapter, Index 14 (95% over 21bp)
ATGCTTGGGATTATTTGTGTGTGTGTGTATGTGTGTATGTGTGTGTGTGTG	377	0.14835627543109894	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	357	0.14048591599178334	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	351	0.13812480815998868	No Hit
TACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	339	0.13340259249639932	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	335	0.1318285206085362	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	321	0.12631926900101528	TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTAT	315	0.12395816116922061	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTATC	294	0.11569428375793923	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	275	0.10821744229058941	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAC	35	1.2069358E-7	45.000004	6
GCTTGTC	35	1.2069358E-7	45.000004	38
CAGGGTA	35	1.2069358E-7	45.000004	5
GGGCGAG	35	1.2069358E-7	45.000004	7
TCGTCCC	35	1.2069358E-7	45.000004	38
TAGGGTG	35	1.2069358E-7	45.000004	5
ACGTCTC	35	1.2069358E-7	45.000004	34
ATAACGT	35	1.2069358E-7	45.000004	31
ACTCCTC	35	1.2069358E-7	45.000004	33
TTACCGG	35	1.2069358E-7	45.000004	2
CGGGTTA	25	3.882203E-5	45.0	6
CTTCGGA	20	7.0229074E-4	45.0	11
TTGATAC	25	3.882203E-5	45.0	31
AATCCGG	40	6.7793735E-9	45.0	2
GGTACGG	20	7.0229074E-4	45.0	8
ATCTCCC	20	7.0229074E-4	45.0	10
AAATCCG	20	7.0229074E-4	45.0	1
ACCGGGT	50	2.1827873E-11	45.0	4
CGAGTAG	20	7.0229074E-4	45.0	1
CGAAAGG	25	3.882203E-5	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content