##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254118
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492125705380964	31.0	31.0	34.0	30.0	34.0
2	31.694818942381097	31.0	31.0	34.0	30.0	34.0
3	31.86036408282766	33.0	31.0	34.0	30.0	34.0
4	35.57351702752265	37.0	35.0	37.0	33.0	37.0
5	35.32558889964505	37.0	35.0	37.0	33.0	37.0
6	35.337587262610285	37.0	35.0	37.0	33.0	37.0
7	35.41747928127877	37.0	35.0	37.0	33.0	37.0
8	35.511234938099626	37.0	35.0	37.0	33.0	37.0
9	37.16676898133938	39.0	37.0	39.0	34.0	39.0
10	36.71613974610221	38.0	37.0	39.0	32.0	39.0
11	36.7771940594527	39.0	37.0	39.0	32.0	39.0
12	36.83888193673805	39.0	37.0	39.0	33.0	39.0
13	36.79343454615572	39.0	37.0	39.0	33.0	39.0
14	37.87137078050355	39.0	37.0	41.0	33.0	41.0
15	37.92452718815668	40.0	37.0	41.0	33.0	41.0
16	37.78418293863481	39.0	37.0	41.0	33.0	41.0
17	37.7233253842703	39.0	37.0	41.0	33.0	41.0
18	37.60175981237063	39.0	36.0	41.0	33.0	41.0
19	37.57243878827946	39.0	36.0	41.0	32.0	41.0
20	37.53093838295595	39.0	36.0	41.0	32.0	41.0
21	37.521895339960174	39.0	36.0	41.0	32.0	41.0
22	37.517633540323786	39.0	36.0	41.0	32.0	41.0
23	37.32691505521057	39.0	36.0	40.0	32.0	41.0
24	37.186787240573274	39.0	36.0	40.0	31.0	41.0
25	37.274663738892954	39.0	36.0	41.0	32.0	41.0
26	37.11907460313712	39.0	36.0	40.0	31.0	41.0
27	37.06785823908578	39.0	36.0	40.0	31.0	41.0
28	36.99640718091595	39.0	36.0	40.0	31.0	41.0
29	36.74300128286859	39.0	35.0	40.0	31.0	41.0
30	36.82332617130624	39.0	35.0	40.0	31.0	41.0
31	36.6117827151166	39.0	35.0	40.0	30.0	41.0
32	36.531571946890814	39.0	35.0	40.0	30.0	41.0
33	36.45210099245232	39.0	35.0	40.0	30.0	41.0
34	36.19248144562762	39.0	35.0	40.0	30.0	41.0
35	36.16159028482831	39.0	35.0	40.0	30.0	41.0
36	36.01890853855296	38.0	35.0	40.0	29.0	41.0
37	35.93566374676332	38.0	35.0	40.0	28.0	41.0
38	35.94125563714495	38.0	35.0	40.0	28.0	41.0
39	35.734501294674125	38.0	35.0	40.0	27.0	41.0
40	35.54982724561031	38.0	34.0	40.0	26.0	41.0
41	35.3925223714967	38.0	34.0	40.0	26.0	41.0
42	35.352060853619186	38.0	34.0	40.0	26.0	41.0
43	35.289345894427	38.0	34.0	40.0	26.0	41.0
44	35.248235071895735	38.0	34.0	40.0	26.0	41.0
45	34.96409542023784	38.0	34.0	40.0	26.0	41.0
46	34.630561392738805	37.0	33.0	40.0	25.0	41.0
47	34.43298782455395	37.0	33.0	40.0	24.0	41.0
48	34.17644952344973	37.0	33.0	40.0	24.0	41.0
49	33.91818761362831	36.0	33.0	39.0	24.0	41.0
50	33.77642669940736	36.0	33.0	39.0	23.0	41.0
51	32.31722664274077	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	7.0
14	5.0
15	6.0
16	34.0
17	62.0
18	194.0
19	332.0
20	503.0
21	884.0
22	1144.0
23	1562.0
24	1811.0
25	2104.0
26	2409.0
27	2848.0
28	3495.0
29	4488.0
30	5662.0
31	7125.0
32	9075.0
33	11817.0
34	16382.0
35	22027.0
36	26412.0
37	36930.0
38	50020.0
39	46768.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.921524646030583	36.41221794599359	19.612542204802494	25.053715203173326
2	19.14307526424732	35.490598855649736	21.12050307337536	24.24582280672758
3	19.350459235473284	32.34284859789547	23.60556906633926	24.70112310029199
4	20.276013505536795	33.13972249112617	18.842034015693496	27.742229987643537
5	16.855161775238276	36.248907987627796	19.43113041972627	27.46479981740766
6	19.50629235237173	35.77786697518476	22.169228468664166	22.546612203779347
7	75.97651484743308	9.416491551169141	8.513761323479644	6.0932322779181325
8	78.12394242045035	6.61661118063262	5.956288023674041	9.303158375242997
9	70.77774891979317	11.231789955847283	12.396996670837957	5.593464453521593
10	25.923389921217705	42.675056469828974	14.399609630171812	17.00194397878151
11	17.557984873169158	28.169196987226407	35.41504340503231	18.85777473457213
12	21.598627409313785	23.041657812512298	36.67469443329477	18.68502034487915
13	23.724411493872925	26.822972005131472	29.129774356794876	20.322842144200727
14	16.447870674253693	30.565721436498006	32.77296374125406	20.213444147994238
15	14.732132316482893	32.41997812040076	30.91477187763165	21.933117685484696
16	16.951180160397925	31.82773357259226	28.165655325478717	23.0554309415311
17	15.51759418852659	31.03243375124942	30.084842474755824	23.365129585468168
18	18.029812921556125	28.788987793072508	28.050748077664707	25.130451207706656
19	18.19745157761355	33.66113380398083	29.655120849369194	18.486293769036433
20	18.504002077774892	31.869839995592596	29.047922618625993	20.57823530800652
21	18.105368372173558	32.811134984534746	26.68287960711166	22.400617036180044
22	17.342730542503876	31.289007468971107	27.35461478525724	24.013647203267773
23	19.00770508189109	30.1678747668406	24.607465822964134	26.216954328304176
24	15.16696967550508	34.02080923035755	26.53609740356842	24.276123690568948
25	19.564926530194633	30.311902344580076	25.412997111578083	24.710174013647205
26	20.50149930347319	33.57023115245673	23.9581611692206	21.970108374849477
27	18.141178507622442	29.498500696526808	25.549547847850214	26.810772948000533
28	24.673576842254384	28.50840947906091	25.66720972146798	21.150803957216727
29	20.79978592622325	33.4348609701005	23.510731235095506	22.254621868580738
30	26.926073713786508	28.846441416979513	22.029923106588278	22.1975617626457
31	25.18357613392204	32.74974618090808	20.394068897126534	21.67260878804335
32	24.186795110932717	29.243894568664953	20.037935132497502	26.531375187904832
33	28.120794276674616	27.82368820784045	22.66388055942515	21.391636956059784
34	22.2125154455804	27.6713967526897	25.17216411273503	24.943923688994875
35	26.582926042232348	30.749494329406023	20.989461588710757	21.67811803965087
36	26.11503317356504	28.145192390936497	27.83037801336387	17.9093964221346
37	25.243390865660835	29.99748148497942	21.695432830417367	23.06369481894238
38	26.806444250308914	27.848479840074297	25.290219504324764	20.05485640529203
39	23.635869950180624	25.406307306054664	26.91820335434719	24.039619389417517
40	25.32209446005399	28.949149607662584	24.73850730762874	20.99024862465469
41	21.30270189439552	25.657765290140798	31.04345225446446	21.99608056099922
42	25.374825868297407	28.761441535034905	24.42408644802808	21.439646148639607
43	21.401474905358928	26.457393809175265	26.95873570545967	25.18239558000614
44	25.24063623985707	23.31200465925279	25.326423157745616	26.120935943144524
45	27.607646841231237	24.708993459731307	25.826978018086088	21.85638168095137
46	20.464508614108407	25.08873830267828	31.287826915055213	23.1589261681581
47	23.34545368686988	25.200497406716565	26.36098190604365	25.093067000369906
48	21.51520159925704	23.69804578975122	33.2302316246783	21.556520986313448
49	23.223069597588523	23.14672711102716	28.49857152976176	25.131631761622554
50	21.330641670405086	21.972863000653238	26.821791451215578	29.874703877726095
51	20.911545030261532	20.84622104691521	33.565508936793144	24.676724986030113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2224.0
1	1823.0
2	1422.0
3	973.0
4	524.0
5	525.5
6	527.0
7	572.5
8	618.0
9	716.0
10	814.0
11	968.5
12	1123.0
13	1130.5
14	1138.0
15	1127.5
16	1117.0
17	1150.0
18	1183.0
19	1163.0
20	1143.0
21	1142.0
22	1141.0
23	1303.5
24	1466.0
25	1475.0
26	1789.0
27	2094.0
28	2225.0
29	2356.0
30	3078.0
31	3800.0
32	4634.0
33	5468.0
34	5895.0
35	6322.0
36	7063.0
37	7804.0
38	8007.5
39	8211.0
40	9111.5
41	10012.0
42	9810.5
43	9609.0
44	12057.0
45	14505.0
46	19464.0
47	24423.0
48	26640.5
49	28858.0
50	31356.0
51	33854.0
52	30246.0
53	26638.0
54	22211.5
55	17785.0
56	14917.0
57	12049.0
58	10110.5
59	8172.0
60	6854.0
61	5536.0
62	4854.0
63	4172.0
64	3163.5
65	2155.0
66	1761.0
67	1367.0
68	1188.0
69	1009.0
70	867.0
71	725.0
72	548.5
73	372.0
74	396.0
75	271.5
76	123.0
77	166.5
78	210.0
79	162.5
80	115.0
81	63.0
82	11.0
83	7.0
84	3.0
85	5.5
86	8.0
87	7.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.10473874341841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92577790170503	29.312366695786995
2	10.220900331505407	7.789294737090644
3	3.829352170276048	4.377493920147333
4	1.9549524429160086	2.9797180837248836
5	1.179374373909182	2.2469876199246017
6	0.7642180706591897	1.7472197955280617
7	0.5628362817692681	1.5012710630494495
8	0.4337453914552158	1.3222203858050197
9	0.37178176410447067	1.2749982291691262
>10	3.264450434261755	26.737972123186864
>50	0.41412357612747985	10.55139738231845
>100	0.06919271720833205	4.783210949244052
>500	0.004130908490049674	1.105391983251875
>1k	0.005163635612562093	4.270457031772641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3140	1.2356464319725482	Illumina PCR Primer Index 8 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGT	2004	0.7886100158194225	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	2002	0.787822979875491	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1875	0.7378461974358369	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1831	0.7205314066693426	No Hit
AAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	891	0.35062451302150965	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	718	0.2825459038714298	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	692	0.27231443660031956	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	508	0.19990712975861608	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	472	0.185740482767848	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	412	0.16212940444990123	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	379	0.14914331137503048	No Hit
AACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	379	0.14914331137503048	TruSeq Adapter, Index 14 (95% over 21bp)
ATGCTTGGGATTATTTGTGTGTGTGTGTATGTGTGTATGTGTGTGTGTGTG	377	0.14835627543109894	No Hit
AACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	357	0.14048591599178334	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	351	0.13812480815998868	No Hit
TACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	339	0.13340259249639932	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	335	0.1318285206085362	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	321	0.12631926900101528	TruSeq Adapter, Index 14 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTAT	315	0.12395816116922061	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTATC	294	0.11569428375793923	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	275	0.10821744229058941	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.412729519357149	0.0
2	0.0	0.0	0.0	2.050228633941712	0.0
3	0.0	0.0	0.0	2.838051613817203	0.0
4	0.0	0.0	0.0	6.493440055407331	0.0
5	0.0	0.0	0.0	7.017212476093783	0.0
6	0.0	0.0	0.0	9.563667272684343	0.0
7	0.0	0.0	0.0	11.484034975877348	0.0
8	0.0	0.0	0.0	13.092736445273456	0.0
9	0.0	0.0	0.0	15.87018629140793	0.0
10	0.0	0.0	0.0	19.036825411816558	0.0
11	0.0	0.0	0.0	22.298302363468938	0.0
12	0.0	0.0	0.0	23.7063096671625	0.0
13	0.0	0.0	0.0	24.319410667485183	0.0
14	0.0	0.0	0.0	24.97894678849983	0.0
15	0.0	0.0	0.0	25.483043310587995	0.0
16	0.0	0.0	0.0	26.48848172896056	0.0
17	0.0	0.0	0.0	27.73435962820422	0.0
18	0.0	0.0	0.0	29.683454143350726	0.0
19	0.0	0.0	0.0	30.359911537159903	0.0
20	0.0	0.0	0.0	31.231553845064106	0.0
21	0.0	0.0	0.0	31.936738050826783	0.0
22	0.0	0.0	0.0	32.75210728873988	0.0
23	0.0	0.0	0.0	33.51120345666187	0.0
24	0.0	0.0	0.0	34.023170338189345	0.0
25	0.0	0.0	0.0	34.471387308258365	0.0
26	0.0	0.0	0.0	34.89835430784124	0.0
27	0.0	0.0	0.0	35.45518223817282	0.0
28	0.0	0.0	0.0	35.95495006256936	0.0
29	0.0	0.0	0.0	36.459440102629486	0.0
30	0.0	0.0	0.0	37.24372142075729	0.0
31	0.0	0.0	0.0	37.705318001873145	0.0
32	0.0	0.0	0.0	38.16258588529738	0.0
33	0.0	0.0	0.0	38.58522418718863	0.0
34	0.0	0.0	0.0	39.01297822271543	0.0
35	0.0	0.0	0.0	39.45883408495266	0.0
36	0.0	0.0	0.0	39.91295382460117	0.0
37	0.0	0.0	0.0	40.3414948960719	0.0
38	0.0	0.0	0.0	40.73973508370127	0.0
39	0.0	0.0	0.0	41.178901140415086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAC	35	1.2069358E-7	45.000004	6
GCTTGTC	35	1.2069358E-7	45.000004	38
CAGGGTA	35	1.2069358E-7	45.000004	5
GGGCGAG	35	1.2069358E-7	45.000004	7
TCGTCCC	35	1.2069358E-7	45.000004	38
TAGGGTG	35	1.2069358E-7	45.000004	5
ACGTCTC	35	1.2069358E-7	45.000004	34
ATAACGT	35	1.2069358E-7	45.000004	31
ACTCCTC	35	1.2069358E-7	45.000004	33
TTACCGG	35	1.2069358E-7	45.000004	2
CGGGTTA	25	3.882203E-5	45.0	6
CTTCGGA	20	7.0229074E-4	45.0	11
TTGATAC	25	3.882203E-5	45.0	31
AATCCGG	40	6.7793735E-9	45.0	2
GGTACGG	20	7.0229074E-4	45.0	8
ATCTCCC	20	7.0229074E-4	45.0	10
AAATCCG	20	7.0229074E-4	45.0	1
ACCGGGT	50	2.1827873E-11	45.0	4
CGAGTAG	20	7.0229074E-4	45.0	1
CGAAAGG	25	3.882203E-5	45.0	2
>>END_MODULE