FastQCFastQC Report
Sat 18 Jun 2016
SRR3553035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554770
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT29180.5259837410097878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14700.26497467418930365No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGT11280.20332750509220038No Hit
AATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT11050.19918164284297998No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC10740.19359374155055248No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9490.17106188150044163No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC6560.11824720154298178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA253.8881866E-545.00000438
CGGCATC253.8881866E-545.00000433
CATTACG253.8881866E-545.0000041
ACGATCA253.8881866E-545.00000436
CCACTAA253.8881866E-545.00000425
TCATGCG253.8881866E-545.0000041
TATTAGG351.2104101E-745.0000042
GGACGTC351.2104101E-745.0000049
CGTACAT253.8881866E-545.00000445
TCGACAG253.8881866E-545.0000041
TAGCTAG253.8881866E-545.0000041
GCATCGT253.8881866E-545.00000435
GTCATAC207.030128E-445.09
AGCCGAT207.030128E-445.018
ATCTCGA207.030128E-445.026
AGTTTCG207.030128E-445.041
CGTATAG207.030128E-445.01
ACGTTCA207.030128E-445.022
TTAGCCG207.030128E-445.01
AGGACGA207.030128E-445.031