##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554770
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82387115381149	33.0	31.0	34.0	30.0	34.0
2	32.02804585684157	33.0	31.0	34.0	30.0	34.0
3	32.16540368080466	34.0	31.0	34.0	30.0	34.0
4	35.82121419687438	37.0	35.0	37.0	35.0	37.0
5	35.59579104854264	37.0	35.0	37.0	33.0	37.0
6	35.61955044432828	37.0	35.0	37.0	33.0	37.0
7	35.700856210681906	37.0	35.0	37.0	35.0	37.0
8	35.72017953386088	37.0	35.0	37.0	35.0	37.0
9	37.396423743172846	39.0	37.0	39.0	35.0	39.0
10	36.992840276150474	39.0	37.0	39.0	33.0	39.0
11	36.977596120914974	39.0	37.0	39.0	33.0	39.0
12	36.82892550065793	39.0	35.0	39.0	33.0	39.0
13	36.803594282315196	39.0	35.0	39.0	33.0	39.0
14	37.94585864412279	40.0	37.0	41.0	33.0	41.0
15	37.969535122663444	40.0	37.0	41.0	33.0	41.0
16	37.877208572922115	40.0	37.0	41.0	33.0	41.0
17	37.84162806207978	40.0	37.0	41.0	33.0	41.0
18	37.78447104205346	40.0	36.0	41.0	33.0	41.0
19	37.795407105647385	40.0	36.0	41.0	33.0	41.0
20	37.72749607945635	40.0	36.0	41.0	33.0	41.0
21	37.704010671088916	40.0	36.0	41.0	33.0	41.0
22	37.698752636227624	40.0	36.0	41.0	33.0	41.0
23	37.55265966076031	39.0	36.0	41.0	33.0	41.0
24	37.44139733583287	39.0	36.0	41.0	32.0	41.0
25	37.454747012275355	39.0	36.0	41.0	32.0	41.0
26	37.38531643744254	39.0	36.0	41.0	32.0	41.0
27	37.32346738287939	39.0	35.0	41.0	32.0	41.0
28	37.255150783207455	39.0	35.0	41.0	32.0	41.0
29	37.04566937649837	39.0	35.0	41.0	31.0	41.0
30	37.05007480577537	39.0	35.0	41.0	31.0	41.0
31	37.005665410891	39.0	35.0	41.0	31.0	41.0
32	36.91346864466355	39.0	35.0	41.0	31.0	41.0
33	36.820851884564775	39.0	35.0	41.0	31.0	41.0
34	36.70293635200173	39.0	35.0	41.0	31.0	41.0
35	36.627517710042	39.0	35.0	41.0	31.0	41.0
36	36.50529228328857	39.0	35.0	40.0	30.0	41.0
37	36.45143573012239	39.0	35.0	40.0	30.0	41.0
38	36.38217819997477	39.0	35.0	40.0	30.0	41.0
39	36.31618148061359	39.0	35.0	40.0	30.0	41.0
40	36.211734592714095	38.0	35.0	40.0	30.0	41.0
41	36.099300611064045	38.0	35.0	40.0	30.0	41.0
42	36.00101663752546	38.0	35.0	40.0	29.0	41.0
43	35.88813922886962	38.0	35.0	40.0	29.0	41.0
44	35.83388431241776	38.0	35.0	40.0	29.0	41.0
45	35.659911314598844	38.0	34.0	40.0	28.0	41.0
46	35.51263045946969	38.0	34.0	40.0	28.0	41.0
47	35.39161634551255	38.0	34.0	40.0	28.0	41.0
48	35.29430394577933	37.0	34.0	40.0	28.0	41.0
49	35.092382428754256	37.0	34.0	40.0	27.0	41.0
50	34.99348558862231	37.0	34.0	40.0	27.0	41.0
51	33.68304162085188	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	8.0
13	11.0
14	16.0
15	22.0
16	59.0
17	127.0
18	276.0
19	530.0
20	773.0
21	1201.0
22	1639.0
23	2198.0
24	2831.0
25	3430.0
26	4207.0
27	5208.0
28	6424.0
29	8213.0
30	10739.0
31	13366.0
32	17838.0
33	24082.0
34	38595.0
35	46639.0
36	50885.0
37	73814.0
38	110059.0
39	131532.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.22135299313229	30.987075725075258	21.244479694287723	27.54709158750473
2	24.235268669899238	30.011356057465257	19.610649458334084	26.14272581430142
3	20.77834057357103	30.856571191665015	21.27061665194585	27.0944715828181
4	20.98220884330443	31.068911440777256	19.468969122338986	28.479910593579323
5	18.43755069668511	33.82753213043243	17.681922238044596	30.052994934837862
6	20.516970996989745	37.002361338933255	19.934206968653676	22.546460695423328
7	84.71294410296159	5.45523370045244	5.418101195089857	4.413721001496115
8	85.18539214449231	5.003334715287417	4.398038826901239	5.413234313319033
9	78.94028155812319	7.613425383492258	8.466211222668854	4.980081835715702
10	37.99484471042053	30.911188420426484	13.751284316022858	17.342682553130125
11	29.061593092632982	27.238855742019215	23.499287993222417	20.200263172125386
12	28.50803035492186	21.83175009463381	29.1865097247508	20.473709825693533
13	27.588189700236132	22.365124285740038	26.77451917010653	23.2721668439173
14	21.94531066928637	25.972925716963786	28.698379508625194	23.383384105124648
15	21.643924509256088	27.902734466535684	28.591308109667068	21.86203291454116
16	24.316383366079634	25.277141878616366	27.36593543270184	23.04053932260216
17	22.736809849126665	25.0817455882618	28.112731402202712	24.068713160408816
18	24.576130648737315	25.37538078843485	26.658975791769564	23.38951277105828
19	24.34792797014979	30.420534635975272	24.64300520936604	20.588532184508896
20	26.05944805955621	27.33348955422968	23.976422661643564	22.630639724570543
21	25.216936748562468	27.999170827550156	24.737458766696108	22.04643365719127
22	23.04666798853579	26.527029219316113	25.30273807163329	25.123564720514807
23	24.07736539466806	26.69106116048092	24.66085044252573	24.570723002325288
24	23.788056311624636	26.445013248733712	24.974133424662472	24.792797014979183
25	25.00639904825423	25.41485660724264	23.535699479063396	26.04304486543973
26	23.098040629450043	26.885015411792274	25.13239720965445	24.88454674910323
27	20.969050237035166	26.48557059682391	25.191520810425942	27.353858355714983
28	23.905402238765614	26.793085422787822	26.442129170647295	22.85938316779927
29	24.17055716783532	29.824071236728734	23.35147899129369	22.653892604142257
30	24.99432197126737	26.106314328460446	24.866881770823944	24.03248192944824
31	25.189718261621934	27.508697297979346	23.17735277682643	24.12423166357229
32	26.430953368062436	25.888926942696973	23.416190493357607	24.263929195882977
33	24.781981722155127	26.576779566306758	25.04893920002884	23.592299511509275
34	23.051354615426213	27.08996521080808	25.110946878886747	24.74773329487896
35	24.069614434810823	27.751500621879337	25.431440056239524	22.747444887070316
36	22.665428916487915	30.43026839951692	24.651477188744884	22.252825495250285
37	23.454945292643796	30.186203291454117	22.795572940137355	23.563278475764733
38	24.310254700146004	27.751140112118534	24.177947617931757	23.760657569803705
39	24.27636678263064	25.344737458766698	23.635200173044684	26.74369558555798
40	24.756926293779404	25.016132811795877	24.19903743893866	26.027903455486058
41	22.663265857923104	24.569641473042882	26.576058546785152	26.19103412224886
42	24.5323287127999	26.878526236097844	22.882455792490582	25.706689258611675
43	21.783261531805973	25.791409052400095	26.04899327649296	26.37633613930097
44	24.086738648448907	23.285505705066964	24.935558880256682	27.692196766227443
45	24.480415307244442	24.805595111487644	26.474935558880258	24.239054022387656
46	22.284550354200842	25.277502388377165	27.955729401373542	24.482217856048454
47	23.220253438361844	25.852515456855997	25.340231086756678	25.587000018025485
48	22.965192782594592	24.352434342159814	27.954107107449932	24.728265767795662
49	24.24139733583287	23.861239793067398	27.039313589415432	24.858049281684302
50	22.79250860717054	22.30257584224093	26.88663770571588	28.018277844872653
51	22.59332696432756	22.654974133424663	29.30295437748977	25.44874452475801
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1846.0
1	1479.0
2	1112.0
3	794.0
4	476.0
5	463.0
6	450.0
7	506.0
8	562.0
9	632.5
10	703.0
11	778.5
12	854.0
13	847.0
14	840.0
15	839.5
16	839.0
17	957.5
18	1076.0
19	1223.5
20	1371.0
21	1353.5
22	1336.0
23	1786.0
24	2236.0
25	2689.5
26	3419.5
27	3696.0
28	4691.0
29	5686.0
30	6220.5
31	6755.0
32	7838.0
33	8921.0
34	10307.0
35	11693.0
36	12374.5
37	13056.0
38	15000.5
39	16945.0
40	17203.5
41	17462.0
42	20105.0
43	22748.0
44	28290.0
45	33832.0
46	38481.0
47	43130.0
48	47843.5
49	52557.0
50	50646.5
51	48736.0
52	45323.5
53	41911.0
54	37403.0
55	32895.0
56	31995.5
57	31096.0
58	31473.0
59	31850.0
60	30559.0
61	29268.0
62	26647.0
63	24026.0
64	21240.0
65	18454.0
66	14966.0
67	11478.0
68	10245.5
69	9013.0
70	8169.0
71	7325.0
72	6557.0
73	5789.0
74	4522.0
75	2953.5
76	2652.0
77	1914.0
78	1176.0
79	1143.0
80	1110.0
81	941.5
82	773.0
83	548.5
84	324.0
85	272.0
86	220.0
87	138.0
88	56.0
89	37.5
90	19.0
91	16.5
92	14.0
93	8.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.185607703111753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6157709233558	21.594620631171825
2	9.387056834900433	5.291598028706349
3	3.3082281948612167	2.7973326627819537
4	1.6393150967275005	1.8482036887260012
5	0.9990647415002135	1.4079623436967887
6	0.6793192644513844	1.148821577579589
7	0.5021723827171791	0.9907823645022314
8	0.4445427330637345	1.0023765665122837
9	0.3494397106293224	0.8864253539718274
>10	4.67490890851267	31.917943407849524
>50	1.2790426601113558	24.41760352328199
>100	0.11598371728969474	4.9150490836581
>500	0.0019330619548282458	0.3837526775955941
>1k	0.0032217699247137424	1.397528089965971
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	2918	0.5259837410097878	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1470	0.26497467418930365	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGT	1128	0.20332750509220038	No Hit
AATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	1105	0.19918164284297998	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	1074	0.19359374155055248	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	949	0.17106188150044163	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	656	0.11824720154298178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6076392018313895	0.0
2	0.0	0.0	0.0	0.8661247003262613	0.0
3	0.0	0.0	0.0	1.1658885664329361	0.0
4	0.0	0.0	0.0	2.436324963498387	0.0
5	0.0	0.0	0.0	2.6519098004578474	0.0
6	0.0	0.0	0.0	3.8323990122032554	0.0
7	0.0	0.0	0.0	4.6644555401337495	0.0
8	0.0	0.0	0.0	5.551309551706113	0.0
9	0.0	0.0	0.0	6.639147754925465	0.0
10	0.0	0.0	0.0	8.165365827279773	0.0
11	0.0	0.0	0.0	9.48573282621627	0.0
12	0.0	0.0	0.0	10.14618670800512	0.0
13	0.0	0.0	0.0	10.450817455882618	0.0
14	0.0	0.0	0.0	10.768967319790184	0.0
15	0.0	0.0	0.0	11.018980838906213	0.0
16	0.0	0.0	0.0	11.528200876038719	0.0
17	0.0	0.0	0.0	12.110784649494384	0.0
18	0.0	0.0	0.0	13.012960325900824	0.0
19	0.0	0.0	0.0	13.357247147466518	0.0
20	0.0	0.0	0.0	13.817798366890784	0.0
21	0.0	0.0	0.0	14.206247634154694	0.0
22	0.0	0.0	0.0	14.636876543432413	0.0
23	1.8025488040088685E-4	0.0	0.0	15.04893920002884	0.0
24	1.8025488040088685E-4	0.0	0.0	15.362582691926384	0.0
25	1.8025488040088685E-4	0.0	0.0	15.646303873677379	0.0
26	1.8025488040088685E-4	0.0	0.0	15.904969627052653	0.0
27	1.8025488040088685E-4	0.0	0.0	16.22528254952503	0.0
28	1.8025488040088685E-4	0.0	0.0	16.523243866827695	0.0
29	1.8025488040088685E-4	0.0	0.0	16.832561241595617	0.0
30	1.8025488040088685E-4	0.0	0.0	17.26030607278692	0.0
31	1.8025488040088685E-4	0.0	0.0	17.551958469275554	0.0
32	1.8025488040088685E-4	0.0	0.0	17.8663229806947	0.0
33	1.8025488040088685E-4	0.0	0.0	18.18104800187465	0.0
34	1.8025488040088685E-4	0.0	0.0	18.466030967788452	0.0
35	1.8025488040088685E-4	0.0	0.0	18.752816482506265	0.0
36	1.8025488040088685E-4	0.0	0.0	19.03094976296483	0.0
37	1.8025488040088685E-4	0.0	0.0	19.31539196423743	0.0
38	1.8025488040088685E-4	0.0	0.0	19.671936117670384	0.0
39	1.8025488040088685E-4	0.0	0.0	19.999639490239197	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	25	3.8881866E-5	45.000004	38
CGGCATC	25	3.8881866E-5	45.000004	33
CATTACG	25	3.8881866E-5	45.000004	1
ACGATCA	25	3.8881866E-5	45.000004	36
CCACTAA	25	3.8881866E-5	45.000004	25
TCATGCG	25	3.8881866E-5	45.000004	1
TATTAGG	35	1.2104101E-7	45.000004	2
GGACGTC	35	1.2104101E-7	45.000004	9
CGTACAT	25	3.8881866E-5	45.000004	45
TCGACAG	25	3.8881866E-5	45.000004	1
TAGCTAG	25	3.8881866E-5	45.000004	1
GCATCGT	25	3.8881866E-5	45.000004	35
GTCATAC	20	7.030128E-4	45.0	9
AGCCGAT	20	7.030128E-4	45.0	18
ATCTCGA	20	7.030128E-4	45.0	26
AGTTTCG	20	7.030128E-4	45.0	41
CGTATAG	20	7.030128E-4	45.0	1
ACGTTCA	20	7.030128E-4	45.0	22
TTAGCCG	20	7.030128E-4	45.0	1
AGGACGA	20	7.030128E-4	45.0	31
>>END_MODULE