FastQCFastQC Report
Sat 18 Jun 2016
SRR3553034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340098
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT37371.098800933848479Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC22100.6498127010449929No Hit
AATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT21810.6412857470493799No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT21550.6336408917429681No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17990.5289651806244082No Hit
AAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT11000.3234361860404942No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT8000.2352263171203594No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC7210.21199771830472391No Hit
CTCCCGGGGAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTC6000.17641973784026957No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT5790.17024504701586013No Hit
CTCCCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGG5250.15436727061023586No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.15230904033543274No Hit
AACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG4920.14466418502902104No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT4820.14172385606501656No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT4680.13760739551541026No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3790.11143846773577029No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGTC3700.10879217166816624No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTAT3560.10467571111855994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC253.8849925E-545.00000441
TGTCAAC351.2085547E-745.00000425
CGGGTAC700.045.0000046
CGCATAG351.2085547E-745.0000041
ATAGCGG502.1827873E-1145.0000042
CACGCGA253.8849925E-545.00000440
GTTAGCG253.8849925E-545.00000439
ATATGTT253.8849925E-545.00000422
AGGCGTT253.8849925E-545.00000441
GCGTTAC253.8849925E-545.00000443
CGTTACG253.8849925E-545.00000444
GTTACGT253.8849925E-545.00000445
CGGATAG351.2085547E-745.00000429
TACGAAA351.2085547E-745.00000420
TGCGAGG502.1827873E-1145.0000042
CGGGCTA253.8849925E-545.0000046
CGGGCCA351.2085547E-745.0000046
CATACGA351.2085547E-745.00000418
ATGACGG253.8849925E-545.0000042
GTTCACC253.8849925E-545.00000440