##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340098
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624978682615012	31.0	31.0	34.0	30.0	34.0
2	31.829213932454763	33.0	31.0	34.0	30.0	34.0
3	32.00620409411405	33.0	31.0	34.0	30.0	34.0
4	35.69603761268811	37.0	35.0	37.0	33.0	37.0
5	35.42775611735441	37.0	35.0	37.0	33.0	37.0
6	34.82171609359655	37.0	35.0	37.0	32.0	37.0
7	35.16852789490088	37.0	35.0	37.0	33.0	37.0
8	35.56392275167746	37.0	35.0	37.0	33.0	37.0
9	37.18271204182324	39.0	37.0	39.0	34.0	39.0
10	36.803553681585896	39.0	37.0	39.0	32.0	39.0
11	36.82245411616652	39.0	37.0	39.0	32.0	39.0
12	36.71597304306406	39.0	35.0	39.0	32.0	39.0
13	36.702768025686716	39.0	35.0	39.0	32.0	39.0
14	37.83139565654606	40.0	37.0	41.0	33.0	41.0
15	37.894136395979984	40.0	37.0	41.0	33.0	41.0
16	37.75846667725185	40.0	37.0	41.0	33.0	41.0
17	37.76921946027321	39.0	37.0	41.0	33.0	41.0
18	37.69914554040306	39.0	36.0	41.0	33.0	41.0
19	37.645325759045924	39.0	36.0	41.0	32.0	41.0
20	37.58695140812354	39.0	36.0	41.0	32.0	41.0
21	37.5389887620627	39.0	36.0	41.0	32.0	41.0
22	37.50319319725491	39.0	36.0	41.0	32.0	41.0
23	37.35911119736076	39.0	36.0	41.0	32.0	41.0
24	37.27170991890573	39.0	36.0	41.0	32.0	41.0
25	37.26086892601544	39.0	36.0	41.0	32.0	41.0
26	37.23421190362778	39.0	36.0	41.0	32.0	41.0
27	37.148815929526194	39.0	35.0	41.0	31.0	41.0
28	37.03043828543537	39.0	35.0	41.0	31.0	41.0
29	36.771260048574234	39.0	35.0	40.0	31.0	41.0
30	36.84578562649589	39.0	35.0	40.0	31.0	41.0
31	36.782195131991365	39.0	35.0	40.0	31.0	41.0
32	36.67515539638575	39.0	35.0	40.0	30.0	41.0
33	36.47091720621703	39.0	35.0	40.0	30.0	41.0
34	36.41053167028327	39.0	35.0	40.0	30.0	41.0
35	36.358570176831385	39.0	35.0	40.0	30.0	41.0
36	36.27732300689801	39.0	35.0	40.0	30.0	41.0
37	36.21628177760528	38.0	35.0	40.0	30.0	41.0
38	35.973572323271526	38.0	35.0	40.0	29.0	41.0
39	35.905456662491396	38.0	35.0	40.0	29.0	41.0
40	35.797990579186	38.0	35.0	40.0	29.0	41.0
41	35.79988709136778	38.0	35.0	40.0	29.0	41.0
42	35.67897194338102	38.0	35.0	40.0	29.0	41.0
43	35.61207063846303	38.0	35.0	40.0	28.0	41.0
44	35.57403748331363	38.0	35.0	40.0	28.0	41.0
45	35.39605937112244	38.0	34.0	40.0	27.0	41.0
46	35.189789413639595	38.0	34.0	40.0	26.0	41.0
47	35.114811025057485	38.0	34.0	40.0	26.0	41.0
48	35.017568465559926	37.0	34.0	40.0	26.0	41.0
49	34.89688266323236	37.0	34.0	40.0	26.0	41.0
50	34.77106304653365	37.0	34.0	40.0	26.0	41.0
51	33.45724173620545	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	3.0
13	4.0
14	8.0
15	8.0
16	33.0
17	83.0
18	183.0
19	367.0
20	611.0
21	998.0
22	1412.0
23	1746.0
24	2198.0
25	2527.0
26	2821.0
27	3463.0
28	4120.0
29	5183.0
30	7011.0
31	9075.0
32	11788.0
33	15834.0
34	23267.0
35	28819.0
36	34024.0
37	47611.0
38	66841.0
39	70040.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.53377555880952	33.99520138313075	20.280330963428188	27.190692094631547
2	22.2512334680004	33.245123464413204	20.60170891919388	23.901934148392524
3	20.016583455356983	31.051638057265844	21.357667495839436	27.574110991537733
4	21.250051455756868	31.765255896829736	18.706960934789386	28.27773171262401
5	17.220036577692312	36.450670100970896	17.811630765250015	28.517662556086776
6	20.906032966968343	37.68031567371757	20.32649412816306	21.08715723115102
7	78.010455809796	8.69866920711089	7.399631870813707	5.891243112279402
8	79.8463972149204	5.822439414521697	5.832142500102912	8.499020870454986
9	73.47823274467947	9.137366288540361	11.00094678592641	6.383454180853754
10	33.53739216343525	30.717028621162136	15.782803780086915	19.962775435315702
11	23.851948556004444	30.453575145987333	26.083364206787458	19.611112091220768
12	24.175090709148538	23.394139336308946	33.15309116783986	19.27767878670266
13	25.990743844421317	24.492646237261027	27.332415950696564	22.184193967621095
14	17.804573975736403	29.061329381531202	31.004592793841773	22.129503848890614
15	17.916894542161376	29.67115360866574	29.62763673999847	22.784315109174415
16	19.555245840904682	29.85139577415921	27.011626060723675	23.58173232421243
17	18.829866685484774	28.12130621173897	27.88490376303301	25.16392333974325
18	21.02246999394292	26.59174708466383	26.622914571682283	25.762868349710967
19	21.285335403324922	30.830525319172708	28.36241318678734	19.52172609071503
20	21.470576128057207	29.171885750577776	26.082188075201852	23.275350046163165
21	21.926327117477904	29.168357355820966	26.615269716375867	22.29004581032526
22	20.17683138389523	27.724361801598363	26.98222277108363	25.11658404342278
23	21.3997142000247	28.081023704932107	24.927226858140887	25.592035236902305
24	18.26620562308511	29.748190227522652	25.372098630394767	26.613505518997467
25	20.86927885491829	27.771995130815235	26.356520767543472	25.002205246723
26	22.969261801010298	27.184811436703537	24.59614581679398	25.249780945492184
27	18.313250886509184	27.590576833736158	26.13805432551794	27.958117954236723
28	21.258284376856082	26.016030673511754	28.368293844715346	24.357391104916818
29	21.305329640280153	30.273627013390257	23.06188216337644	25.359161182953148
30	21.656698951478692	25.768160941846176	26.1136495951167	26.461490511558434
31	22.208304665125933	26.71465283535922	22.259760421996013	28.817282077518836
32	23.026892248704787	27.109539015225025	23.25947226975754	26.604096466312647
33	21.832824656422563	23.57526360049162	27.465024786973167	27.12688695611265
34	21.441760904209964	25.64878358590759	26.207740121964846	26.701715387917602
35	22.475874600850343	23.876059253509283	26.93223717869555	26.71582896694482
36	22.83518280025169	24.9328134831725	26.908420514087116	25.32358320248869
37	23.07570170950726	26.83667648736541	22.70345606266429	27.384165740463047
38	23.503225540873512	26.96957935653841	27.0295620674041	22.497633035183977
39	23.99220224758746	23.63142388370411	27.066610212350557	25.309763656357877
40	26.26360637228093	24.297114361154726	25.5885068421455	23.850772424418846
41	23.68405577215979	22.223006309945955	29.053684526224792	25.039253391669458
42	26.379749366359107	23.60643108751007	25.328581761727502	24.68523778440332
43	21.20859281736441	23.300930908150004	27.31124558215573	28.17923069232986
44	24.016018912195896	21.802539268093316	25.76610271157137	28.41533910813942
45	25.05189680621468	23.18567001276103	26.91871166546113	24.84372151556316
46	20.3532511217355	23.84930225993684	31.60412586960229	24.193320748725366
47	21.519973654652482	24.051596892660353	27.45885009614876	26.96957935653841
48	22.941916741645056	22.387076666137407	30.485624731694983	24.185381860522558
49	23.92751501037936	22.194779151891513	28.273909284970806	25.603796552758322
50	21.402948561885104	21.74579091908803	27.981640585948757	28.869619933078113
51	21.74579091908803	20.72108627513246	31.465930408294078	26.067192397485428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2149.0
1	1763.0
2	1377.0
3	949.0
4	521.0
5	486.5
6	452.0
7	502.5
8	553.0
9	580.5
10	608.0
11	720.5
12	833.0
13	831.0
14	829.0
15	876.0
16	923.0
17	911.5
18	900.0
19	1078.0
20	1256.0
21	1217.0
22	1178.0
23	1478.0
24	1778.0
25	1765.0
26	2112.0
27	2472.0
28	3103.0
29	3734.0
30	4518.5
31	5303.0
32	5607.0
33	5911.0
34	6681.5
35	7452.0
36	7649.0
37	7846.0
38	8796.0
39	9746.0
40	10052.5
41	10359.0
42	12303.5
43	14248.0
44	15283.5
45	16319.0
46	23377.0
47	30435.0
48	31934.0
49	33433.0
50	35000.0
51	36567.0
52	33967.0
53	31367.0
54	28310.0
55	25253.0
56	22043.5
57	18834.0
58	16791.5
59	14749.0
60	13498.5
61	12248.0
62	11192.0
63	10136.0
64	8783.5
65	7431.0
66	6477.5
67	5524.0
68	5185.5
69	4847.0
70	4111.0
71	3375.0
72	3234.5
73	3094.0
74	2516.5
75	1535.0
76	1131.0
77	856.0
78	581.0
79	435.0
80	289.0
81	251.0
82	213.0
83	141.0
84	69.0
85	60.0
86	51.0
87	34.0
88	17.0
89	11.0
90	5.0
91	7.5
92	10.0
93	5.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.272904762670137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6713033938951	22.90790160910504
2	10.482759595957592	6.346871657967815
3	3.726661178366623	3.3845057680629855
4	1.9151209440360493	2.31905095791193
5	1.1807368234834148	1.787216670354554
6	0.6792673093902962	1.233803673934058
7	0.535421997249387	1.1346145391398528
8	0.4295362662070106	1.0402648383198208
9	0.32664734378341287	0.8899707536400988
>10	3.973709884797881	29.50481359423147
>50	0.9759463442482211	19.732372259809143
>100	0.09090186010909737	4.600450241961572
>500	0.005993529237962464	1.1318928058674924
>1k	0.005993529237962464	3.9862706296941717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3737	1.098800933848479	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC	2210	0.6498127010449929	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	2181	0.6412857470493799	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT	2155	0.6336408917429681	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1799	0.5289651806244082	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	1100	0.3234361860404942	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	800	0.2352263171203594	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	721	0.21199771830472391	No Hit
CTCCCGGGGAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTC	600	0.17641973784026957	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	579	0.17024504701586013	No Hit
CTCCCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGG	525	0.15436727061023586	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	518	0.15230904033543274	No Hit
AACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	492	0.14466418502902104	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	482	0.14172385606501656	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	468	0.13760739551541026	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	379	0.11143846773577029	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGTC	370	0.10879217166816624	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTAT	356	0.10467571111855994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2222947503366677	0.0
2	0.0	0.0	0.0	1.7024504701586014	0.0
3	0.0	0.0	0.0	2.2640533022834597	0.0
4	0.0	0.0	0.0	4.842133737922599	0.0
5	0.0	0.0	0.0	5.201147904427548	0.0
6	0.0	0.0	0.0	7.040323671412358	0.0
7	0.0	0.0	0.0	8.484319225634964	0.0
8	0.0	0.0	0.0	9.66015677834036	0.0
9	0.0	0.0	0.0	11.653405783039007	0.0
10	0.0	0.0	0.0	14.020076566166223	0.0
11	0.0	0.0	0.0	16.245611559021224	0.0
12	0.0	0.0	0.0	17.255908591053167	0.0
13	0.0	0.0	0.0	17.731065751636294	0.0
14	0.0	0.0	0.0	18.264147392810308	0.0
15	0.0	0.0	0.0	18.6510946844733	0.0
16	0.0	0.0	0.0	19.418520544078472	0.0
17	0.0	0.0	0.0	20.30708795700063	0.0
18	0.0	0.0	0.0	22.018065381154845	0.0
19	0.0	0.0	0.0	22.51880340372481	0.0
20	0.0	0.0	0.0	23.28564119753718	0.0
21	0.0	0.0	0.0	23.84195143752683	0.0
22	0.0	0.0	0.0	24.39120488800287	0.0
23	0.0	0.0	0.0	24.9354597792401	0.0
24	0.0	0.0	0.0	25.31917270904269	0.0
25	0.0	0.0	0.0	25.657310539903204	0.0
26	0.0	0.0	0.0	25.96192862057407	0.0
27	0.0	0.0	0.0	26.43355738640039	0.0
28	0.0	0.0	0.0	26.808743362207363	0.0
29	0.0	0.0	0.0	27.171285923469117	0.0
30	0.0	0.0	0.0	27.769348834747632	0.0
31	0.0	0.0	0.0	28.103370205058543	0.0
32	0.0	0.0	0.0	28.462972437356292	0.0
33	0.0	0.0	0.0	28.7972878405636	0.0
34	0.0	0.0	0.0	29.113373204194087	0.0
35	0.0	0.0	0.0	29.432692929684972	0.0
36	0.0	0.0	0.0	29.74524989855865	0.0
37	0.0	0.0	0.0	30.051044110815116	0.0
38	2.9403289640044926E-4	0.0	0.0	30.37271609947721	0.0
39	2.9403289640044926E-4	0.0	0.0	30.689095496004093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	25	3.8849925E-5	45.000004	41
TGTCAAC	35	1.2085547E-7	45.000004	25
CGGGTAC	70	0.0	45.000004	6
CGCATAG	35	1.2085547E-7	45.000004	1
ATAGCGG	50	2.1827873E-11	45.000004	2
CACGCGA	25	3.8849925E-5	45.000004	40
GTTAGCG	25	3.8849925E-5	45.000004	39
ATATGTT	25	3.8849925E-5	45.000004	22
AGGCGTT	25	3.8849925E-5	45.000004	41
GCGTTAC	25	3.8849925E-5	45.000004	43
CGTTACG	25	3.8849925E-5	45.000004	44
GTTACGT	25	3.8849925E-5	45.000004	45
CGGATAG	35	1.2085547E-7	45.000004	29
TACGAAA	35	1.2085547E-7	45.000004	20
TGCGAGG	50	2.1827873E-11	45.000004	2
CGGGCTA	25	3.8849925E-5	45.000004	6
CGGGCCA	35	1.2085547E-7	45.000004	6
CATACGA	35	1.2085547E-7	45.000004	18
ATGACGG	25	3.8849925E-5	45.000004	2
GTTCACC	25	3.8849925E-5	45.000004	40
>>END_MODULE