##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347810
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63430033639056	31.0	31.0	34.0	30.0	34.0
2	31.83431758718841	33.0	31.0	34.0	30.0	34.0
3	31.999833242287455	33.0	31.0	34.0	30.0	34.0
4	35.68394813260113	37.0	35.0	37.0	33.0	37.0
5	35.435591271096285	37.0	35.0	37.0	33.0	37.0
6	35.446033754061126	37.0	35.0	37.0	33.0	37.0
7	35.48064747994595	37.0	35.0	37.0	33.0	37.0
8	35.54112302694	37.0	35.0	37.0	35.0	37.0
9	37.20834938615911	39.0	37.0	39.0	34.0	39.0
10	36.81433541301285	39.0	37.0	39.0	32.0	39.0
11	36.84277622840057	39.0	37.0	39.0	33.0	39.0
12	36.751674764957876	39.0	35.0	39.0	32.0	39.0
13	36.74706592679912	39.0	35.0	39.0	33.0	39.0
14	37.84519134009948	40.0	37.0	41.0	33.0	41.0
15	37.915022569793855	40.0	37.0	41.0	33.0	41.0
16	37.79152410798999	40.0	37.0	41.0	33.0	41.0
17	37.76703947557574	39.0	37.0	41.0	33.0	41.0
18	37.65722377159943	39.0	36.0	41.0	33.0	41.0
19	37.64112015180702	39.0	36.0	41.0	32.0	41.0
20	37.593461947615076	39.0	36.0	41.0	32.0	41.0
21	37.5236652195164	39.0	36.0	41.0	32.0	41.0
22	37.49571317673443	39.0	36.0	41.0	32.0	41.0
23	37.32095396912107	39.0	36.0	41.0	32.0	41.0
24	37.257252522929186	39.0	36.0	41.0	32.0	41.0
25	37.24978580259337	39.0	36.0	41.0	32.0	41.0
26	37.20401368563296	39.0	36.0	41.0	32.0	41.0
27	37.15158276070268	39.0	36.0	41.0	32.0	41.0
28	37.0130128518444	39.0	35.0	41.0	31.0	41.0
29	36.79797015611972	39.0	35.0	40.0	31.0	41.0
30	36.854225007906614	39.0	35.0	40.0	31.0	41.0
31	36.77082890083666	39.0	35.0	40.0	31.0	41.0
32	36.63341767056726	39.0	35.0	40.0	30.0	41.0
33	36.57791610361979	39.0	35.0	40.0	30.0	41.0
34	36.46824415629223	39.0	35.0	40.0	30.0	41.0
35	36.39305655386562	39.0	35.0	40.0	30.0	41.0
36	36.259590006037776	39.0	35.0	40.0	30.0	41.0
37	36.21902187976194	38.0	35.0	40.0	30.0	41.0
38	36.06655357810299	38.0	35.0	40.0	30.0	41.0
39	35.933762686524254	38.0	35.0	40.0	29.0	41.0
40	35.80386130358529	38.0	35.0	40.0	29.0	41.0
41	35.74720105804894	38.0	35.0	40.0	29.0	41.0
42	35.61939564704868	38.0	35.0	40.0	28.0	41.0
43	35.540806762312755	38.0	35.0	40.0	28.0	41.0
44	35.5195537793623	38.0	35.0	40.0	28.0	41.0
45	35.35338546907794	38.0	34.0	40.0	27.0	41.0
46	35.14508783531238	38.0	34.0	40.0	26.0	41.0
47	35.06720048302234	38.0	34.0	40.0	26.0	41.0
48	34.974747707081455	37.0	34.0	40.0	26.0	41.0
49	34.76140133981197	37.0	34.0	40.0	26.0	41.0
50	34.65739340444495	37.0	33.0	40.0	25.0	41.0
51	33.2524740519249	36.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	3.0
14	6.0
15	17.0
16	27.0
17	89.0
18	199.0
19	348.0
20	596.0
21	977.0
22	1351.0
23	1854.0
24	2212.0
25	2554.0
26	2869.0
27	3576.0
28	4432.0
29	5570.0
30	7110.0
31	9169.0
32	11973.0
33	15965.0
34	23670.0
35	29458.0
36	34490.0
37	48154.0
38	69567.0
39	71547.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.511831172191716	34.23334579224289	20.132831143440384	27.12199189212501
2	21.792070383255226	33.59679135160001	20.537362353008824	24.073775912135936
3	20.02357609039418	31.069836979960325	21.6632644259797	27.243322503665794
4	21.336074293436074	31.546246513901266	18.825508179753314	28.292171012909346
5	17.02596245076335	36.669733475173224	17.921278859147236	28.38302521491619
6	21.29898507805986	36.22006267789885	20.97064489232627	21.510307351715017
7	77.54952416549266	8.843621517495185	7.611914551047986	5.994939765964176
8	79.1742618096087	5.924786521376613	5.990627066501826	8.910324602512866
9	72.78140363991835	9.123084442655474	11.531008309134297	6.564503608291883
10	33.1885224691642	30.757885052183664	16.004140191483856	20.049452287168283
11	23.686495500416893	30.3395532043357	26.49923808976165	19.474713205485752
12	23.95790805324746	23.373393519450275	33.36160547425319	19.30709295304908
13	25.846007877864352	24.305799143210375	27.46614530922055	22.382047669704725
14	17.69011816796527	28.850234323337453	31.443891779994825	22.01575572870245
15	17.564474856962136	29.790115292832294	29.68891061211581	22.956499238089762
16	19.31830597165119	29.73376268652425	27.34337713119232	23.60455421063224
17	18.537132342370835	28.087749058393953	28.289295879934446	25.085822719300765
18	20.73200885540956	26.560765935424513	26.8287283286852	25.87849688048072
19	20.87404042436963	30.996808602397863	28.800494522871684	19.32865645036083
20	21.267646128633448	29.231189442511717	26.15910985883097	23.342054570023862
21	21.687415542968864	29.40599752738564	26.619418648112475	22.28716828153302
22	19.96348581121877	27.82323682470314	27.14039274316437	25.072884620913715
23	21.34929990512061	28.06647307437969	24.796871855323307	25.78735516517639
24	17.921278859147236	29.98361174204307	25.528593197435384	26.566516201374313
25	20.752422299531354	27.654179005779017	26.655932836893708	24.937465857795925
26	22.85644461056324	27.348552370547136	24.546735286507	25.248267732382622
27	18.196716598142665	27.668554670653517	26.26663983209224	27.86808889911158
28	21.358787843937783	25.882234553348095	28.252781691153217	24.50619591156091
29	21.173054253759236	30.39935597021362	22.958224317874702	25.46936545815244
30	21.443604266697335	25.716339380696358	26.17147293062304	26.668583421983268
31	22.255254305511627	26.785313820764213	21.987291912250942	28.97213996147322
32	22.8892211264771	27.004973980046575	23.279951697766023	26.8258531957103
33	21.556884505908396	23.47546074005923	27.70420631954228	27.263448434490094
34	21.29610994508496	25.53808113625255	26.17779822316782	26.98801069549467
35	22.390960581926915	23.58442827980794	27.01704953854116	27.00756159972399
36	22.6244213794888	24.79629682872833	27.04436330180271	25.534918489980164
37	23.116356631494206	26.813490123918232	22.433225036657944	27.636928207929618
38	23.477185819844166	26.75857508409764	27.275811506282167	22.488427589776027
39	24.051349874931716	23.63589316005865	27.06391420603203	25.248842758977602
40	26.46473649406285	24.2014318162215	25.48345360972945	23.850378079986196
41	23.637905753141084	21.90190046289641	29.440786636381933	25.019407147580573
42	26.669445961875738	23.331416578016732	25.321871136540064	24.677266323567466
43	21.144015410712747	23.092205514505046	27.577412955349185	28.186366119433025
44	24.013685632960524	21.568385037808	25.984301773957046	28.433627555274434
45	25.048445990627066	23.087317788447717	27.0043989534516	24.85983726747362
46	20.178258244443807	23.57379028780081	31.90420056927633	24.343750898479055
47	21.66125183289727	23.648256231850723	27.5828757080015	27.10761622725051
48	22.88577096690722	22.20235185877347	30.63252925447802	24.279347919841292
49	23.807251085362697	21.861361087950318	28.382737701618698	25.948650125068284
50	21.483568615048444	21.535321008596647	27.957218021333485	29.02389235502142
51	21.550846726661106	20.39303067766884	31.88321209855956	26.17291049711049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2292.0
1	1803.5
2	1315.0
3	932.5
4	550.0
5	515.5
6	481.0
7	509.0
8	537.0
9	574.0
10	611.0
11	772.5
12	934.0
13	893.5
14	853.0
15	918.0
16	983.0
17	952.0
18	921.0
19	1107.5
20	1294.0
21	1265.5
22	1237.0
23	1469.0
24	1701.0
25	1769.5
26	2154.0
27	2470.0
28	3096.5
29	3723.0
30	4495.0
31	5267.0
32	5626.5
33	5986.0
34	6752.0
35	7518.0
36	7832.5
37	8147.0
38	8959.0
39	9771.0
40	10123.0
41	10475.0
42	12512.5
43	14550.0
44	15667.0
45	16784.0
46	24177.5
47	31571.0
48	33140.0
49	34709.0
50	36346.0
51	37983.0
52	35239.0
53	32495.0
54	29261.0
55	26027.0
56	22650.5
57	19274.0
58	17032.5
59	14791.0
60	13592.5
61	12394.0
62	11307.0
63	10220.0
64	8800.0
65	7380.0
66	6410.5
67	5441.0
68	5113.0
69	4785.0
70	4074.5
71	3364.0
72	3151.0
73	2938.0
74	2381.0
75	1492.5
76	1161.0
77	855.5
78	550.0
79	419.0
80	288.0
81	262.5
82	237.0
83	150.0
84	63.0
85	56.0
86	49.0
87	31.5
88	14.0
89	9.0
90	4.0
91	4.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.67963814142392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.97506693354094	22.25232856216533
2	10.802279644047964	6.4121550187562635
3	3.903217927485001	3.475382870246204
4	1.9523469070745103	2.317797989139986
5	1.1133029064786166	1.6521213703040425
6	0.7608254939487012	1.354861520950054
7	0.534174172801501	1.10978673072701
8	0.3963870851890948	0.9411700201876889
9	0.36243956543677613	0.9681367635268605
>10	4.076696252568978	29.019803058579047
>50	1.0044468356413176	19.894069507182554
>100	0.10583634649898577	4.961293427811215
>500	0.006989192693329249	1.350412982351912
>1k	0.005990736594282214	4.29068017807183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3967	1.1405652511428654	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGT	2626	0.7550099192087634	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC	2455	0.7058451453379718	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	2395	0.6885943474885714	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1949	0.5603634168080274	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	1087	0.3125269543716397	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	925	0.26594980017825826	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	795	0.2285730715045571	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	668	0.19205888272332594	No Hit
CTCCCGGGGAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTC	613	0.1762456513613755	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	534	0.15353210085966476	No Hit
CTCCCGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGG	518	0.14893188809982463	No Hit
AACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	504	0.1449067019349645	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	467	0.13426870992783416	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	450	0.129380983870504	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	399	0.11471780569851356	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTAT	383	0.1101175929386734	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGTC	371	0.10666743336879331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3104856099594606	0.0
2	0.0	0.0	0.0	1.8288720853339466	0.0
3	0.0	0.0	0.0	2.4289123371955954	0.0
4	0.0	0.0	0.0	5.127224634139329	0.0
5	0.0	0.0	0.0	5.48891636238176	0.0
6	0.0	0.0	0.0	7.468445415600471	0.0
7	0.0	0.0	0.0	8.972427474770708	0.0
8	0.0	0.0	0.0	10.240936143296627	0.0
9	0.0	0.0	0.0	12.430349903683044	0.0
10	0.0	0.0	0.0	14.87881314510796	0.0
11	0.0	0.0	0.0	17.196745349472412	0.0
12	0.0	0.0	0.0	18.247893965095887	0.0
13	0.0	0.0	0.0	18.71682815330209	0.0
14	0.0	0.0	0.0	19.28265432276243	0.0
15	0.0	0.0	0.0	19.697248497743022	0.0
16	0.0	0.0	0.0	20.495672924872775	0.0
17	0.0	0.0	0.0	21.433541301285185	0.0
18	0.0	0.0	0.0	23.189960035651648	0.0
19	0.0	0.0	0.0	23.712946723785976	0.0
20	0.0	0.0	0.0	24.53408470141744	0.0
21	0.0	0.0	0.0	25.09502314482045	0.0
22	0.0	0.0	0.0	25.671774819585405	0.0
23	0.0	0.0	0.0	26.236738449153272	0.0
24	0.0	0.0	0.0	26.638682039044305	0.0
25	0.0	0.0	0.0	26.994335988039445	0.0
26	0.0	0.0	0.0	27.289612144561687	0.0
27	0.0	0.0	0.0	27.75078347373566	0.0
28	0.0	0.0	0.0	28.129150973232512	0.0
29	0.0	0.0	0.0	28.513268738679162	0.0
30	0.0	0.0	0.0	29.13602254104252	0.0
31	0.0	0.0	0.0	29.495701676202525	0.0
32	0.0	0.0	0.0	29.841292659785516	0.0
33	0.0	0.0	0.0	30.164170092866794	0.0
34	0.0	0.0	0.0	30.480434720105805	0.0
35	0.0	0.0	0.0	30.825163163796326	0.0
36	0.0	0.0	0.0	31.136252551680514	0.0
37	0.0	0.0	0.0	31.459417498059285	0.0
38	0.0	0.0	0.0	31.785170064115466	0.0
39	2.875132974900089E-4	0.0	0.0	32.10747247060176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	7.0264953E-4	45.0	30
ACAACGT	20	7.0264953E-4	45.0	28
TTAGGTC	35	1.2086639E-7	45.0	25
AGACTAC	20	7.0264953E-4	45.0	26
TCGTTGT	20	7.0264953E-4	45.0	32
TCGCACG	20	7.0264953E-4	45.0	37
ATTAGGT	35	1.2086639E-7	45.0	24
GAAGATC	20	7.0264953E-4	45.0	20
TACTTCG	20	7.0264953E-4	45.0	28
CAACGTG	20	7.0264953E-4	45.0	29
GTACAAC	20	7.0264953E-4	45.0	12
CGAAAGT	20	7.0264953E-4	45.0	16
ACTTGCA	20	7.0264953E-4	45.0	10
GCGAAGC	20	7.0264953E-4	45.0	30
GCGAACG	20	7.0264953E-4	45.0	40
GGCACGC	20	7.0264953E-4	45.0	8
CGTGATT	20	7.0264953E-4	45.0	5
CGTTGTG	20	7.0264953E-4	45.0	33
CTATAAC	20	7.0264953E-4	45.0	28
CGCATAG	20	7.0264953E-4	45.0	1
>>END_MODULE