SRR3553032_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417401
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2827	0.6772863505358157	Illumina Single End Adapter 1 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTC	1975	0.47316609207931937	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1271	0.3045033433077544	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGT	1217	0.29156614382811735	No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGT	691	0.16554823778572644	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	643	0.1540485049149379	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	634	0.15189230500166506	No Hit
AGCCCAGGGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	625	0.14973610508839222	No Hit
AGCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	617	0.1478194829432608	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	609	0.1459028607981294	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	582	0.13943426105831083	No Hit
AGCCCAGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	559	0.133923972391058	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	536	0.12841368372380516	No Hit
AGCCCAGGGAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGAC	529	0.12673663934681517	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
AGCCCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	509	0.12194508398398662	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	476	0.11403901763531951	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	443	0.1061329512866524	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.1621672E-6	45.000004	1
TACCACG	25	3.886522E-5	45.000004	1
CCGATCA	25	3.886522E-5	45.000004	22
TAGCGTG	25	3.886522E-5	45.000004	1
ACTCGCA	30	2.1621672E-6	45.000004	12
TTACGCG	30	2.1621672E-6	45.000004	1
TCGAGTG	25	3.886522E-5	45.000004	39
ATACGAG	25	3.886522E-5	45.000004	1
GATTAGC	25	3.886522E-5	45.000004	9
TACGTTC	25	3.886522E-5	45.000004	21
TATCCGG	50	2.1827873E-11	45.000004	2
CTTCGGC	20	7.028119E-4	45.0	35
CGGAACA	20	7.028119E-4	45.0	41
CGGAAAT	20	7.028119E-4	45.0	16
CGAACAG	35	1.209446E-7	45.0	1
CGTGCGT	20	7.028119E-4	45.0	14
CGTGACA	20	7.028119E-4	45.0	18
CGTTGAT	45	3.8380676E-10	45.0	25
ATTACGG	55	1.8189894E-12	45.0	2
GTCTACG	20	7.028119E-4	45.0	25