##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417401
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717453959142407	31.0	31.0	34.0	30.0	34.0
2	31.92754928713635	33.0	31.0	34.0	30.0	34.0
3	32.10721823857633	34.0	31.0	34.0	30.0	34.0
4	35.78615527993464	37.0	35.0	37.0	35.0	37.0
5	35.55068627051684	37.0	35.0	37.0	33.0	37.0
6	34.95287505300658	37.0	35.0	37.0	33.0	37.0
7	35.349678127268504	37.0	35.0	37.0	33.0	37.0
8	35.702442016190666	37.0	35.0	37.0	35.0	37.0
9	37.217785774351285	39.0	37.0	39.0	34.0	39.0
10	36.88846217426408	39.0	37.0	39.0	33.0	39.0
11	36.9187256379357	39.0	37.0	39.0	33.0	39.0
12	36.83055862348198	39.0	37.0	39.0	33.0	39.0
13	36.790141853996516	39.0	37.0	39.0	33.0	39.0
14	37.941126159256925	40.0	37.0	41.0	33.0	41.0
15	37.97151420336798	40.0	37.0	41.0	33.0	41.0
16	37.85486139228224	40.0	37.0	41.0	33.0	41.0
17	37.83282263339091	40.0	37.0	41.0	33.0	41.0
18	37.794133219613755	40.0	37.0	41.0	33.0	41.0
19	37.75692439644371	40.0	36.0	41.0	33.0	41.0
20	37.626035874375	39.0	36.0	41.0	32.0	41.0
21	37.5463475171358	39.0	36.0	41.0	32.0	41.0
22	37.525396441311834	39.0	36.0	41.0	32.0	41.0
23	37.38061959602397	39.0	36.0	41.0	32.0	41.0
24	37.28340372926754	39.0	36.0	41.0	32.0	41.0
25	37.21246235634318	39.0	36.0	41.0	31.0	41.0
26	37.221602248197776	39.0	36.0	41.0	32.0	41.0
27	37.145967546795525	39.0	35.0	41.0	31.0	41.0
28	36.92027570609558	39.0	35.0	41.0	31.0	41.0
29	36.65827106307843	39.0	35.0	41.0	30.0	41.0
30	36.763711634615156	39.0	35.0	41.0	31.0	41.0
31	36.66407124084513	39.0	35.0	41.0	30.0	41.0
32	36.63392277450222	39.0	35.0	41.0	30.0	41.0
33	36.35404323420404	39.0	35.0	40.0	30.0	41.0
34	36.396894113813815	39.0	35.0	40.0	30.0	41.0
35	36.19653283053946	39.0	35.0	40.0	30.0	41.0
36	36.19184429361693	39.0	35.0	40.0	30.0	41.0
37	36.12184685709905	38.0	35.0	40.0	30.0	41.0
38	35.943572248269646	38.0	35.0	40.0	29.0	41.0
39	35.888795187361794	38.0	35.0	40.0	29.0	41.0
40	35.79466029070366	38.0	35.0	40.0	29.0	41.0
41	35.7598520367704	38.0	35.0	40.0	29.0	41.0
42	35.71256178111696	38.0	35.0	40.0	29.0	41.0
43	35.67619387591309	38.0	35.0	40.0	28.0	41.0
44	35.62875268626573	38.0	35.0	40.0	28.0	41.0
45	35.39221036844665	38.0	34.0	40.0	27.0	41.0
46	35.240758886538366	38.0	34.0	40.0	27.0	41.0
47	35.026468551824266	37.0	34.0	40.0	26.0	41.0
48	34.98712029918472	37.0	34.0	40.0	26.0	41.0
49	34.83322751981907	37.0	34.0	40.0	26.0	41.0
50	34.795029240466604	37.0	34.0	40.0	26.0	41.0
51	33.499632248125906	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	1.0
12	5.0
13	12.0
14	13.0
15	26.0
16	62.0
17	102.0
18	229.0
19	444.0
20	898.0
21	1370.0
22	1887.0
23	2209.0
24	2658.0
25	2999.0
26	3431.0
27	4222.0
28	5101.0
29	6505.0
30	8303.0
31	10650.0
32	13814.0
33	18886.0
34	28442.0
35	34916.0
36	41497.0
37	58718.0
38	81662.0
39	88312.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.368187905635107	34.7792650233229	20.134355212373713	26.718191858668284
2	26.353075340020748	30.59096648067446	18.687305492799492	24.368652686505303
3	20.399567801706272	30.215548117996843	19.221563915754874	30.16332016454201
4	19.604409189244876	31.249565765295245	18.456831679847436	30.689193365612443
5	18.765168267445453	31.949851581572634	17.666943778285148	31.61803637269676
6	20.066075548453405	39.6000488738647	18.102735738534406	22.231139839147488
7	84.35844667358248	5.8341978097800435	5.35025071813436	4.457104798503118
8	86.22092424311394	4.444886332327905	4.403918533975721	4.930270890582437
9	78.16727798927171	8.430262505360552	8.511239790992354	4.891219714375385
10	35.00494728091212	35.303461180016335	12.258236084724281	17.433355454347257
11	26.367689583877375	31.419186825139374	22.71364946418432	19.49947412679893
12	26.310909652827856	23.384227637212177	31.291971030256278	19.01289167970369
13	28.614689471275824	23.816665508707455	25.547614883529267	22.021030136487454
14	21.773067146461077	28.236396175380506	28.069650048754074	21.920886629404336
15	20.14417790086751	27.01622660223622	30.42757444280201	22.412021054094264
16	22.368178322524383	28.35187265962468	25.67267447849909	23.607274539351845
17	22.030852824981253	25.31115162637368	26.404584560171156	26.253410988473913
18	26.27161889885266	24.818579735074902	24.94747257433499	23.96232879173744
19	25.7340063871433	28.47932803227592	24.926629308506687	20.860036272074097
20	25.429503043835545	26.46759351319235	24.177230049760304	23.925673393211806
21	26.369127050486224	26.791742233487703	25.43573206580722	21.403398650218854
22	23.088109515789373	26.522217244328594	23.906267593992347	26.483405645889686
23	22.90579083423375	27.7881461711879	23.878955728424227	25.42710726615413
24	20.89573335952717	26.845647231319525	25.472627042101003	26.78599236705231
25	23.41489359153428	27.47286182831378	24.110867007985128	25.00137757216681
26	23.090265715702646	27.527964714986307	23.069662027642483	26.31210754166856
27	21.803014367478752	25.596728325998257	23.976463880057786	28.6237934264652
28	23.453944767741334	25.741912453491967	25.469512531115164	25.33463024765154
29	23.282167507983928	30.195184007704825	22.200473884825385	24.322174599485866
30	23.927590015356934	24.729217227558152	24.499941303446807	26.843251453638107
31	25.614217503072584	28.34732068202999	21.50474004614268	24.533721768754745
32	25.440044465633765	25.54929192790626	22.74719035172412	26.263473254735853
33	24.438609394802597	25.731610609461885	23.827206930505675	26.00257306522984
34	24.52916979116006	25.006887860834066	24.013358856351566	26.450583491654307
35	23.933100304024187	24.204781493096565	26.33510700741014	25.527011195469107
36	25.081875702262334	27.22298221614227	24.127877029523166	23.56726505207223
37	25.90075251376973	25.860982604258254	23.368894660051126	24.869370221920885
38	26.443156580841926	25.598644948143388	25.98388599931481	21.974312471699875
39	25.6738723673398	23.460413367481152	25.337026025332953	25.528688239846097
40	26.829116365317763	23.58643127352354	25.912731402176803	23.67172095898189
41	22.786001950163033	23.382550592835187	28.319529660925586	25.511917796076194
42	24.3813503082168	24.419443173351286	24.357152953634515	26.842053564797403
43	22.268034815441265	24.277133979075277	27.39691567581295	26.05791552967051
44	25.358348446697544	21.138186060886294	24.966399218018164	28.537066274397997
45	26.431656847971137	22.264920304455426	26.81977283236025	24.483650015213186
46	23.78935364313933	23.410581191707735	28.15062733438588	24.649437830767056
47	23.385904681589167	25.36433789090108	26.14200732628815	25.107750101221608
48	23.80899902012693	23.23353322105122	29.340849686512488	23.61661807230936
49	24.244551402608046	22.850448369793078	27.88996672264801	25.015033504950875
50	23.303489929348515	22.46041576325883	26.771138545427537	27.464955761965115
51	23.47263183365636	21.269714255595936	28.619241448870508	26.638412461877188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1518.0
1	1266.0
2	1014.0
3	684.5
4	355.0
5	342.0
6	329.0
7	389.0
8	449.0
9	487.0
10	525.0
11	583.5
12	642.0
13	657.5
14	673.0
15	677.0
16	681.0
17	693.5
18	706.0
19	774.0
20	842.0
21	944.0
22	1046.0
23	1372.0
24	1698.0
25	1832.0
26	2134.5
27	2303.0
28	3017.5
29	3732.0
30	4122.5
31	4513.0
32	5385.5
33	6258.0
34	7281.0
35	8304.0
36	8834.0
37	9364.0
38	10341.5
39	11319.0
40	12304.0
41	13289.0
42	13691.0
43	14093.0
44	16630.5
45	19168.0
46	23356.5
47	27545.0
48	30315.0
49	33085.0
50	39017.5
51	44950.0
52	42501.0
53	40052.0
54	36559.5
55	33067.0
56	30134.0
57	27201.0
58	24705.5
59	22210.0
60	20870.0
61	19530.0
62	18388.5
63	17247.0
64	15042.5
65	12838.0
66	11541.0
67	10244.0
68	8853.0
69	7462.0
70	6508.5
71	5555.0
72	4782.5
73	4010.0
74	3494.5
75	2639.0
76	2299.0
77	1730.0
78	1161.0
79	934.5
80	708.0
81	487.0
82	266.0
83	198.0
84	130.0
85	82.5
86	35.0
87	24.5
88	14.0
89	11.5
90	9.0
91	8.5
92	8.0
93	7.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.596528667547155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46310061634803	22.926388780688345
2	9.245342001405216	5.472600591717345
3	3.1915328960035563	2.8337488454996715
4	1.5636394844238861	1.8511320330584078
5	0.9399327134253187	1.3909372749228914
6	0.6175996577625621	1.0967283585622187
7	0.4672316255209275	0.9679903939380351
8	0.3741023348591159	0.885770438260332
9	0.3324426718927663	0.8855234162091209
>10	4.663991014398706	34.3854687797915
>50	1.047151717563799	20.264743150158758
>100	0.08168110121402487	3.697047658189319
>500	0.008984921133542736	1.5795795068730145
>1k	0.003267244048560995	1.7623407721310491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2827	0.6772863505358157	Illumina Single End Adapter 1 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTC	1975	0.47316609207931937	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1271	0.3045033433077544	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGT	1217	0.29156614382811735	No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGT	691	0.16554823778572644	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	643	0.1540485049149379	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	634	0.15189230500166506	No Hit
AGCCCAGGGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	625	0.14973610508839222	No Hit
AGCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	617	0.1478194829432608	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	609	0.1459028607981294	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	582	0.13943426105831083	No Hit
AGCCCAGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	559	0.133923972391058	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	536	0.12841368372380516	No Hit
AGCCCAGGGAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGAC	529	0.12673663934681517	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
AGCCCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	509	0.12194508398398662	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	476	0.11403901763531951	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	443	0.1061329512866524	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7503575698189511	0.0
2	0.0	0.0	0.0	1.0057474706577127	0.0
3	0.0	0.0	0.0	1.3155215248645786	0.0
4	0.0	0.0	0.0	2.721603446086617	0.0
5	0.0	0.0	0.0	2.9707643249537017	0.0
6	0.0	0.0	0.0	4.279338094542179	0.0
7	0.0	0.0	0.0	5.148286659591137	0.0
8	0.0	0.0	0.0	5.92260200622423	0.0
9	0.0	0.0	0.0	6.959255008972187	0.0
10	0.0	0.0	0.0	8.619289364424139	0.0
11	0.0	0.0	0.0	10.075922194724019	0.0
12	0.0	0.0	0.0	10.825561031238545	0.0
13	2.3957776814142754E-4	0.0	0.0	11.184208950146262	0.0
14	2.3957776814142754E-4	0.0	0.0	11.546690113344242	0.0
15	2.3957776814142754E-4	0.0	0.0	11.859818256305088	0.0
16	2.3957776814142754E-4	0.0	0.0	12.587176360382461	0.0
17	2.3957776814142754E-4	0.0	0.0	13.270931310658096	0.0
18	2.3957776814142754E-4	0.0	0.0	14.695700297795165	0.0
19	2.3957776814142754E-4	0.0	0.0	15.073035282617914	0.0
20	2.3957776814142754E-4	0.0	0.0	15.906765915750082	0.0
21	2.3957776814142754E-4	0.0	0.0	16.34734943136217	0.0
22	2.3957776814142754E-4	0.0	0.0	16.848306544545892	0.0
23	2.3957776814142754E-4	0.0	0.0	17.338243080395113	0.0
24	2.3957776814142754E-4	0.0	0.0	17.653767001037373	0.0
25	2.3957776814142754E-4	0.0	0.0	17.94509356709735	0.0
26	2.3957776814142754E-4	0.0	0.0	18.21869137831486	0.0
27	2.3957776814142754E-4	0.0	0.0	18.720127647034865	0.0
28	2.3957776814142754E-4	0.0	0.0	19.058890611186843	0.0
29	2.3957776814142754E-4	0.0	0.0	19.36387311003088	0.0
30	2.3957776814142754E-4	0.0	0.0	20.060804837554294	0.0
31	2.3957776814142754E-4	0.0	0.0	20.345183648338168	0.0
32	2.3957776814142754E-4	0.0	0.0	20.621656392773374	0.0
33	2.3957776814142754E-4	0.0	0.0	20.921368180718304	0.0
34	2.3957776814142754E-4	0.0	0.0	21.16118552662787	0.0
35	2.3957776814142754E-4	0.0	0.0	21.47000126976217	0.0
36	2.3957776814142754E-4	0.0	0.0	21.73856794784871	0.0
37	2.3957776814142754E-4	0.0	0.0	22.015040692283918	0.0
38	2.3957776814142754E-4	0.0	0.0	22.282888637066034	0.0
39	2.3957776814142754E-4	0.0	0.0	22.558403070428678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.1621672E-6	45.000004	1
TACCACG	25	3.886522E-5	45.000004	1
CCGATCA	25	3.886522E-5	45.000004	22
TAGCGTG	25	3.886522E-5	45.000004	1
ACTCGCA	30	2.1621672E-6	45.000004	12
TTACGCG	30	2.1621672E-6	45.000004	1
TCGAGTG	25	3.886522E-5	45.000004	39
ATACGAG	25	3.886522E-5	45.000004	1
GATTAGC	25	3.886522E-5	45.000004	9
TACGTTC	25	3.886522E-5	45.000004	21
TATCCGG	50	2.1827873E-11	45.000004	2
CTTCGGC	20	7.028119E-4	45.0	35
CGGAACA	20	7.028119E-4	45.0	41
CGGAAAT	20	7.028119E-4	45.0	16
CGAACAG	35	1.209446E-7	45.0	1
CGTGCGT	20	7.028119E-4	45.0	14
CGTGACA	20	7.028119E-4	45.0	18
CGTTGAT	45	3.8380676E-10	45.0	25
ATTACGG	55	1.8189894E-12	45.0	2
GTCTACG	20	7.028119E-4	45.0	25
>>END_MODULE