SRR3553031_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422404
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	3084	0.7301067224742189	Illumina Single End Adapter 1 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTC	2174	0.5146731565041998	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGT	1360	0.32196664804310565	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1274	0.3016069923580269	No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGT	804	0.19033910663724776	No Hit
AGCCCAGGGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	779	0.18442060207763183	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	729	0.1725835929584	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	729	0.1725835929584	No Hit
AGCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	662	0.15672200073862935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	646	0.1529341578204752	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	600	0.1420441094307819	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	566	0.13399494322970426	No Hit
AGCCCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	541	0.12807643867008836	No Hit
AGCCCAGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	511	0.12097423319854925	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	501	0.1186068313747029	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	489	0.11576594918608725	No Hit
AGCCCAGGGAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGAC	426	0.10085131769585515	Illumina DpnII expression PCR Primer 2 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.028213E-4	45.000004	1
TATCATG	20	7.028213E-4	45.000004	26
AACGTCG	20	7.028213E-4	45.000004	37
CGAACAG	40	6.7975634E-9	45.000004	1
ATAGGCG	20	7.028213E-4	45.000004	1
CGCATAG	40	6.7975634E-9	45.000004	1
CGTCTAC	20	7.028213E-4	45.000004	24
TAAGTCG	20	7.028213E-4	45.000004	1
CGACAAT	20	7.028213E-4	45.000004	35
TAGTTCA	20	7.028213E-4	45.000004	34
CGCCTCG	20	7.028213E-4	45.000004	1
TGCATAA	20	7.028213E-4	45.000004	31
ATTCCCG	20	7.028213E-4	45.000004	1
TACCTAG	20	7.028213E-4	45.000004	1
GGTGCGT	20	7.028213E-4	45.000004	8
CGAGCAT	20	7.028213E-4	45.000004	35
ACGTCGT	20	7.028213E-4	45.000004	38
CTCGACG	20	7.028213E-4	45.000004	1
AAGATCG	20	7.028213E-4	45.000004	40
ACGAGCA	20	7.028213E-4	45.000004	34