##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422404
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718828420185414	31.0	31.0	34.0	30.0	34.0
2	31.93109440251513	33.0	31.0	34.0	30.0	34.0
3	32.08496368405602	34.0	31.0	34.0	30.0	34.0
4	35.76889423395612	37.0	35.0	37.0	35.0	37.0
5	35.55981950928495	37.0	35.0	37.0	33.0	37.0
6	35.55984318330319	37.0	35.0	37.0	33.0	37.0
7	35.66062584634615	37.0	35.0	37.0	35.0	37.0
8	35.68501008513177	37.0	35.0	37.0	35.0	37.0
9	37.224306588005796	39.0	37.0	39.0	34.0	39.0
10	36.88037281843922	39.0	37.0	39.0	32.0	39.0
11	36.91150888722645	39.0	37.0	39.0	33.0	39.0
12	36.873808013181694	39.0	37.0	39.0	33.0	39.0
13	36.81676783363794	39.0	37.0	39.0	33.0	39.0
14	37.949122167403715	40.0	37.0	41.0	33.0	41.0
15	37.984467476633746	40.0	37.0	41.0	33.0	41.0
16	37.87878665921724	40.0	37.0	41.0	33.0	41.0
17	37.84652844196551	40.0	37.0	41.0	33.0	41.0
18	37.75670684936696	40.0	37.0	41.0	33.0	41.0
19	37.734912548176624	40.0	36.0	41.0	33.0	41.0
20	37.61082518157972	39.0	36.0	41.0	32.0	41.0
21	37.52610060510791	39.0	36.0	41.0	32.0	41.0
22	37.49944839537504	39.0	36.0	41.0	32.0	41.0
23	37.33187185727408	39.0	36.0	41.0	32.0	41.0
24	37.24687502959252	39.0	36.0	41.0	31.0	41.0
25	37.18766630997813	39.0	36.0	41.0	31.0	41.0
26	37.173042868912226	39.0	35.0	41.0	31.0	41.0
27	37.124177327866214	39.0	35.0	41.0	31.0	41.0
28	36.92116551926592	39.0	35.0	41.0	31.0	41.0
29	36.69846166229487	39.0	35.0	41.0	30.0	41.0
30	36.790091949886836	39.0	35.0	41.0	31.0	41.0
31	36.66219069895171	39.0	35.0	40.0	30.0	41.0
32	36.6205623052812	39.0	35.0	40.0	30.0	41.0
33	36.47083360953021	39.0	35.0	40.0	30.0	41.0
34	36.47080046590468	39.0	35.0	40.0	30.0	41.0
35	36.24103228189127	39.0	35.0	40.0	30.0	41.0
36	36.17704377799453	38.0	35.0	40.0	30.0	41.0
37	36.10580155490952	38.0	35.0	40.0	30.0	41.0
38	36.05494502892965	38.0	35.0	40.0	30.0	41.0
39	35.9588095756669	38.0	35.0	40.0	29.0	41.0
40	35.81260120642797	38.0	35.0	40.0	29.0	41.0
41	35.7403599397733	38.0	35.0	40.0	29.0	41.0
42	35.689467902766076	38.0	35.0	40.0	28.0	41.0
43	35.63415829395555	38.0	35.0	40.0	28.0	41.0
44	35.62697322942017	38.0	35.0	40.0	28.0	41.0
45	35.39890957471994	38.0	34.0	40.0	27.0	41.0
46	35.23875484133673	38.0	34.0	40.0	27.0	41.0
47	34.98953134913495	37.0	34.0	40.0	26.0	41.0
48	34.99277705703545	37.0	34.0	40.0	26.0	41.0
49	34.75163113985663	37.0	33.0	40.0	26.0	41.0
50	34.75446255243795	37.0	34.0	40.0	26.0	41.0
51	33.36336303633488	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	2.0
12	2.0
13	8.0
14	15.0
15	16.0
16	63.0
17	122.0
18	221.0
19	440.0
20	799.0
21	1296.0
22	1772.0
23	2222.0
24	2689.0
25	2990.0
26	3485.0
27	4235.0
28	5318.0
29	6551.0
30	8442.0
31	10937.0
32	14117.0
33	18991.0
34	28521.0
35	35192.0
36	41808.0
37	58744.0
38	84108.0
39	89270.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.354939820645637	34.97102300167612	20.114866336493026	26.559170841185214
2	26.3020710031155	30.5981950928495	18.74934896449844	24.350384939536557
3	20.4280735977879	30.212071855380156	19.256919915531103	30.102934631300837
4	19.729216579388453	31.193833391729246	18.451293074876183	30.625656954006118
5	18.61393358017443	31.957083739737314	17.59571405573811	31.833268624350147
6	20.466898987698983	38.329892709349345	18.65417941117982	22.549028891771858
7	84.0976884688592	5.84582532362383	5.450469219041486	4.606016988475488
8	85.68976619539588	4.6024658857397185	4.555354589445176	5.152413329419229
9	77.84206588952756	8.396227308453518	8.731214666527778	5.030492135491142
10	34.748487230234566	35.31263908485715	12.470762587475498	17.46811109743279
11	26.14724292383595	31.466794822018727	22.868864878173504	19.51709737597182
12	26.185121353017493	23.382827814130547	31.459455876364807	18.972594956487153
13	28.784055075236033	23.758061003210194	25.44365110178881	22.014232819764963
14	21.59591291749131	28.3129421122906	28.144619842615125	21.94652512760296
15	19.904641054535468	27.04496169543849	30.495449853694566	22.554947396331475
16	22.089752937945665	28.36526169259761	25.7873978466113	23.757587522845427
17	21.801876876165945	25.30231721290518	26.571718070851603	26.32408784007727
18	26.00259467239894	24.853221086921526	25.019649435137925	24.124534805541614
19	25.45430440999612	28.590401606045397	25.333566916979954	20.621727066978533
20	25.367894243425727	26.574322212857833	24.226569824149394	23.83121371956705
21	26.0108805787824	26.816507419437315	25.771299514209144	21.40131248757114
22	22.931837766687817	26.59373490781337	23.94248160528783	26.53194572021098
23	22.91076789045558	27.721091656329012	24.031022433499682	25.33711801971572
24	20.760220073673544	26.91262393348548	25.54118805693128	26.7859679359097
25	23.243387846706	27.400545449380214	24.21733695703639	25.1387297468774
26	22.96640183331597	27.618583157356465	23.317250783610003	26.097764225717558
27	21.49222071760684	25.767748411473374	23.974678270092138	28.765352600827644
28	23.379276711394777	25.81485970776792	25.47584776659312	25.330015814244184
29	23.301862671755003	30.249240064014543	22.120055681290896	24.328841582939557
30	23.925909792520905	24.739822539559285	24.37192829613356	26.962339371786253
31	25.538347174742665	28.64674576945294	21.333604795409133	24.48130226039526
32	25.279826895578637	25.431814092669576	22.899877842065887	26.388481169685896
33	24.324816999839015	25.708326625694834	23.91596670486075	26.050889669605404
34	24.702180850560126	24.95099478224638	23.737464607342734	26.609359759850758
35	23.84257724832151	24.149155784509617	26.401738619899433	25.606528347269435
36	25.136599085235932	27.077158360242805	24.490535127508263	23.295707427013003
37	25.906714898533156	25.744547873599682	23.404134430545163	24.944602797321995
38	26.495250991941365	25.575278643194665	26.07314324674956	21.856327118114415
39	25.553735286597668	23.30517703430839	25.615997954564822	25.525089724529128
40	26.668781545629304	23.485809793467865	25.96755712540601	23.877851535496823
41	22.891591935682428	23.25001657181277	28.306313387183835	25.552078105320973
42	24.365062830844405	24.13234723156031	24.620268747455043	26.882321190140246
43	22.09164685940474	24.256399087129857	27.345621727066977	26.306332326398422
44	25.131390801223475	20.985833467486103	24.969933996837153	28.91284173445327
45	26.517741309267905	22.2294296455526	26.811772615789625	24.441056429389874
46	23.67970000284088	23.292156324277233	28.288321133322604	24.739822539559285
47	23.34921070823193	25.251654813874868	26.192697038853797	25.2064374390394
48	23.96710258425583	23.03363604511321	29.536888855219175	23.462372515411786
49	24.068664122498838	22.65887633639833	28.15811403301105	25.11434550809178
50	23.352051590420544	22.190840995823905	26.722758307213002	27.73434910654255
51	23.44722114373917	20.854679406445015	28.946932320716662	26.75116712909916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1558.0
1	1282.5
2	1007.0
3	691.5
4	376.0
5	379.0
6	382.0
7	445.5
8	509.0
9	512.5
10	516.0
11	587.5
12	659.0
13	680.5
14	702.0
15	709.0
16	716.0
17	720.5
18	725.0
19	763.0
20	801.0
21	954.5
22	1108.0
23	1386.0
24	1664.0
25	1784.5
26	2081.5
27	2258.0
28	3003.5
29	3749.0
30	4145.5
31	4542.0
32	5398.0
33	6254.0
34	7281.0
35	8308.0
36	8930.0
37	9552.0
38	10388.0
39	11224.0
40	12265.5
41	13307.0
42	13729.0
43	14151.0
44	16663.5
45	19176.0
46	23817.0
47	28458.0
48	31173.5
49	33889.0
50	40126.5
51	46364.0
52	43810.5
53	41257.0
54	37270.5
55	33284.0
56	30487.5
57	27691.0
58	24945.0
59	22199.0
60	20949.0
61	19699.0
62	18464.5
63	17230.0
64	15002.5
65	12775.0
66	11439.5
67	10104.0
68	8744.5
69	7385.0
70	6371.0
71	5357.0
72	4659.0
73	3961.0
74	3457.5
75	2629.5
76	2305.0
77	1705.0
78	1105.0
79	910.5
80	716.0
81	503.0
82	290.0
83	217.5
84	145.0
85	98.5
86	52.0
87	33.5
88	15.0
89	13.0
90	11.0
91	7.0
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.340863403932616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45359135211021	22.725552440062817
2	9.163574494085367	5.3773437504543935
3	3.2330879468168296	2.8458477546136063
4	1.6085810509504854	1.8878862756037025
5	0.9191712572982488	1.3484639152604458
6	0.6299975624287754	1.1090803454419924
7	0.46290833935534814	0.9507491247496589
8	0.39317568775676026	0.922889131857469
9	0.32345546774502565	0.8541416426725736
>10	4.632941581515963	33.77764267401566
>50	1.0781823338255667	20.6411127401956
>100	0.08917297497805439	4.000969778783478
>500	0.008917297497805439	1.6811636880352903
>1k	0.0032426536355656143	1.8771567382533265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	3084	0.7301067224742189	Illumina Single End Adapter 1 (95% over 21bp)
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTC	2174	0.5146731565041998	No Hit
AGCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGT	1360	0.32196664804310565	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1274	0.3016069923580269	No Hit
AGCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGT	804	0.19033910663724776	No Hit
AGCCCAGGGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	779	0.18442060207763183	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	729	0.1725835929584	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	729	0.1725835929584	No Hit
AGCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	662	0.15672200073862935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	646	0.1529341578204752	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	600	0.1420441094307819	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	566	0.13399494322970426	No Hit
AGCCCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	541	0.12807643867008836	No Hit
AGCCCAGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	511	0.12097423319854925	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	501	0.1186068313747029	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	489	0.11576594918608725	No Hit
AGCCCAGGGAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGAC	426	0.10085131769585515	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7348036476927303E-4	0.0	0.0	0.8319050008996127	0.0
2	4.7348036476927303E-4	0.0	0.0	1.1065236124657911	0.0
3	4.7348036476927303E-4	0.0	0.0	1.4500336171058985	0.0
4	4.7348036476927303E-4	0.0	0.0	2.9590155396255717	0.0
5	4.7348036476927303E-4	0.0	0.0	3.2080662114942093	0.0
6	4.7348036476927303E-4	0.0	0.0	4.5688487798411	0.0
7	4.7348036476927303E-4	0.0	0.0	5.486690466946336	0.0
8	4.7348036476927303E-4	0.0	0.0	6.308652380185793	0.0
9	4.7348036476927303E-4	0.0	0.0	7.396710258425583	0.0
10	4.7348036476927303E-4	0.0	0.0	9.137934299864584	0.0
11	4.7348036476927303E-4	0.0	0.0	10.716754576187725	0.0
12	4.7348036476927303E-4	0.0	0.0	11.480241664378179	0.0
13	4.7348036476927303E-4	0.0	0.0	11.830380394125056	0.0
14	4.7348036476927303E-4	0.0	0.0	12.205613583204704	0.0
15	4.7348036476927303E-4	0.0	0.0	12.512902339939963	0.0
16	4.7348036476927303E-4	0.0	0.0	13.286805996155339	0.0
17	4.7348036476927303E-4	0.0	0.0	14.000577646045018	0.0
18	4.7348036476927303E-4	0.0	0.0	15.52684160187877	0.0
19	4.7348036476927303E-4	0.0	0.0	15.929536652115036	0.0
20	4.7348036476927303E-4	0.0	0.0	16.831753487182887	0.0
21	4.7348036476927303E-4	0.0	0.0	17.28795181863808	0.0
22	4.7348036476927303E-4	0.0	0.0	17.783922500733894	0.0
23	4.7348036476927303E-4	0.0	0.0	18.29173019194894	0.0
24	4.7348036476927303E-4	0.0	0.0	18.62411340801697	0.0
25	4.7348036476927303E-4	0.0	0.0	18.92193255745684	0.0
26	4.7348036476927303E-4	0.0	0.0	19.185424380450943	0.0
27	4.7348036476927303E-4	0.0	0.0	19.67689699908145	0.0
28	4.7348036476927303E-4	0.0	0.0	20.02679898864594	0.0
29	4.7348036476927303E-4	0.0	0.0	20.337402107934583	0.0
30	4.7348036476927303E-4	0.0	0.0	21.010454446454105	0.0
31	4.7348036476927303E-4	0.0	0.0	21.30282857169913	0.0
32	4.7348036476927303E-4	0.0	0.0	21.599227280044698	0.0
33	4.7348036476927303E-4	0.0	0.0	21.8965729491198	0.0
34	4.7348036476927303E-4	0.0	0.0	22.16243217393775	0.0
35	4.7348036476927303E-4	0.0	0.0	22.470431151220158	0.0
36	4.7348036476927303E-4	0.0	0.0	22.737710817132413	0.0
37	4.7348036476927303E-4	0.0	0.0	23.01564379125198	0.0
38	4.7348036476927303E-4	0.0	0.0	23.292156324277233	0.0
39	4.7348036476927303E-4	0.0	0.0	23.58263652806318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.028213E-4	45.000004	1
TATCATG	20	7.028213E-4	45.000004	26
AACGTCG	20	7.028213E-4	45.000004	37
CGAACAG	40	6.7975634E-9	45.000004	1
ATAGGCG	20	7.028213E-4	45.000004	1
CGCATAG	40	6.7975634E-9	45.000004	1
CGTCTAC	20	7.028213E-4	45.000004	24
TAAGTCG	20	7.028213E-4	45.000004	1
CGACAAT	20	7.028213E-4	45.000004	35
TAGTTCA	20	7.028213E-4	45.000004	34
CGCCTCG	20	7.028213E-4	45.000004	1
TGCATAA	20	7.028213E-4	45.000004	31
ATTCCCG	20	7.028213E-4	45.000004	1
TACCTAG	20	7.028213E-4	45.000004	1
GGTGCGT	20	7.028213E-4	45.000004	8
CGAGCAT	20	7.028213E-4	45.000004	35
ACGTCGT	20	7.028213E-4	45.000004	38
CTCGACG	20	7.028213E-4	45.000004	1
AAGATCG	20	7.028213E-4	45.000004	40
ACGAGCA	20	7.028213E-4	45.000004	34
>>END_MODULE