FastQCFastQC Report
Sat 18 Jun 2016
SRR3553026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504716
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGT39520.783014606234001No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC37050.7340761933443758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36170.7166406454322827No Hit
AATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT34560.6847415180022032No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT27600.5468421845156484No Hit
AATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT9180.18188446571933523No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8810.17455361034720518No Hit
AAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT8810.17455361034720518No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7170.1420600892383043No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT6500.1287852970779607No Hit
AACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG6360.12601145991012766No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT5870.11630302982271218No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5830.11551050491761704No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5580.1105572242607724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACG351.2101191E-745.0000041
GACGTGT253.8876846E-545.00000440
CGGTTCG253.8876846E-545.00000429
CGCATAG351.2101191E-745.0000041
CGGGCTC351.2101191E-745.0000046
GTATCGG253.8876846E-545.0000042
GCGATAG351.2101191E-745.0000041
CGTAACG253.8876846E-545.0000041
TTTGCGG351.2101191E-745.0000042
AACTCGT253.8876846E-545.00000441
TAAGCGA253.8876846E-545.00000413
TAGTACG253.8876846E-545.0000041
TACCCTA207.029522E-445.026
ACCGGCC207.029522E-445.018
GATCGCC207.029522E-445.033
ACCGCGT207.029522E-445.042
CGGCATT207.029522E-445.033
TTATGCC207.029522E-445.043
TAGCGCA207.029522E-445.025
TCTTACG207.029522E-445.01