##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504716
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53869502849127	31.0	31.0	34.0	30.0	34.0
2	31.72837001402769	31.0	31.0	34.0	30.0	34.0
3	31.925807781009517	33.0	31.0	34.0	30.0	34.0
4	35.71772244192774	37.0	35.0	37.0	35.0	37.0
5	35.23386221162	37.0	35.0	37.0	33.0	37.0
6	35.269333645059795	37.0	35.0	37.0	32.0	37.0
7	35.35801321931542	37.0	35.0	37.0	33.0	37.0
8	35.696062736271486	37.0	35.0	37.0	35.0	37.0
9	37.336777514483394	39.0	37.0	39.0	34.0	39.0
10	36.55486649917974	38.0	35.0	39.0	32.0	39.0
11	36.55544702367272	38.0	35.0	39.0	32.0	39.0
12	36.1611757899492	38.0	35.0	39.0	32.0	39.0
13	35.970525998779515	38.0	35.0	39.0	31.0	39.0
14	36.8878597072413	39.0	35.0	40.0	31.0	41.0
15	36.942751963480454	39.0	35.0	40.0	31.0	41.0
16	36.93957195729876	38.0	35.0	40.0	32.0	41.0
17	36.85139563635787	38.0	35.0	40.0	32.0	41.0
18	36.74481688712068	38.0	35.0	40.0	31.0	41.0
19	36.85688387132566	39.0	35.0	40.0	31.0	41.0
20	36.773474587688916	39.0	35.0	40.0	31.0	41.0
21	36.84229546913512	39.0	35.0	40.0	31.0	41.0
22	36.89955341221598	39.0	35.0	40.0	31.0	41.0
23	36.738924464451294	38.0	35.0	40.0	31.0	41.0
24	36.36106245888777	38.0	35.0	40.0	30.0	41.0
25	36.53086290111667	38.0	35.0	40.0	31.0	41.0
26	36.43781057069718	38.0	35.0	40.0	31.0	41.0
27	36.367735518588674	38.0	35.0	40.0	30.0	41.0
28	36.2165812060644	38.0	35.0	40.0	30.0	41.0
29	35.95633385904152	38.0	34.0	40.0	30.0	41.0
30	36.03458776817062	38.0	35.0	40.0	30.0	41.0
31	35.798082485992126	38.0	34.0	40.0	29.0	41.0
32	35.81824828220227	38.0	34.0	40.0	30.0	41.0
33	35.786515585002256	38.0	34.0	40.0	29.0	41.0
34	35.57256358031051	38.0	34.0	40.0	29.0	41.0
35	35.571862195769505	38.0	34.0	40.0	29.0	41.0
36	35.264404140150106	38.0	34.0	40.0	27.0	41.0
37	35.29764659729432	38.0	34.0	40.0	27.0	41.0
38	35.182072690384295	38.0	34.0	40.0	27.0	41.0
39	35.06848802098606	38.0	34.0	40.0	26.0	41.0
40	34.96858233144977	37.0	34.0	40.0	26.0	41.0
41	34.87870208196293	37.0	34.0	40.0	26.0	41.0
42	34.87096902020146	37.0	33.0	40.0	26.0	41.0
43	34.7640534478796	37.0	33.0	40.0	26.0	41.0
44	34.65714580080679	37.0	33.0	40.0	26.0	41.0
45	34.396759761925516	37.0	33.0	40.0	24.0	41.0
46	34.27079189088517	36.0	33.0	40.0	24.0	41.0
47	34.17832999151998	36.0	33.0	40.0	24.0	41.0
48	34.15858819613406	36.0	33.0	40.0	23.0	41.0
49	34.00148400288479	36.0	33.0	40.0	24.0	41.0
50	33.87232423778917	36.0	33.0	39.0	23.0	41.0
51	32.22130861712329	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	6.0
12	12.0
13	11.0
14	31.0
15	49.0
16	90.0
17	221.0
18	404.0
19	739.0
20	1228.0
21	1817.0
22	2549.0
23	3112.0
24	3725.0
25	4437.0
26	5183.0
27	6203.0
28	7740.0
29	9767.0
30	12879.0
31	16672.0
32	22004.0
33	30379.0
34	45884.0
35	51035.0
36	53446.0
37	71140.0
38	90012.0
39	63929.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.532901671435027	33.6510037327923	18.718645733442173	26.097448862330495
2	24.383217492609706	33.68369538512748	18.623542744830758	23.30954437743206
3	20.76118054509863	31.266098162134746	22.54277653175251	25.429944761014116
4	22.283422756560125	33.46693982358396	18.362009526149357	25.88762789370656
5	18.253433614151323	36.59959264219878	18.002797612914986	27.14417613073491
6	20.338170377004097	36.58948794965882	20.691438353450256	22.380903319886826
7	81.27501406731706	9.056578352974743	5.40957687095317	4.258830708755022
8	83.14378779353142	6.033293971263047	4.1978062910626965	6.625111944142844
9	78.71218665546564	8.76433479422091	7.666885931890411	4.856592618423035
10	37.247085489661515	35.85759119980345	12.351500645907798	14.543822664627237
11	32.073086646747875	28.363277565997514	23.525507414070486	16.038128373184126
12	29.55503689203433	27.48951885813012	27.257507192163516	15.697937057672037
13	25.10778338709294	33.80732927032232	23.51005317842113	17.574834164163608
14	18.518731326131924	33.65873085061698	29.9921143771943	17.83042344605679
15	18.620174513984104	31.290072040513873	29.478161976240102	20.61159146926192
16	21.726674010730786	30.229079323817754	28.610941598839744	19.43330506661172
17	21.410258442371553	29.398116960825494	25.95598316677102	23.23564143003194
18	24.500313047337514	28.581221914898673	24.436316661251077	22.482148376512733
19	22.575468184087686	32.10023062474738	24.856751123404052	20.46755006776088
20	26.32411098518771	31.756869209614912	23.259417177184794	18.659602628012586
21	21.882603285808255	31.662360614682317	25.017633679138367	21.43740242037106
22	22.64481411328351	28.719121248385232	27.157450922895254	21.47861371543601
23	21.987810966959636	31.3102814255938	23.258426521053423	23.443481086393138
24	19.201689663097664	33.0478922800149	26.887596192710355	20.862821864177082
25	21.316740503570326	31.09828101348085	24.878545558294167	22.706432924654656
26	23.66261422265195	32.722957068925886	21.543204495201262	22.0712242132209
27	19.57160066255082	30.027777997923582	26.68371916087463	23.716902178650965
28	21.707059019329684	32.412881699807414	25.067760879385638	20.812298401477268
29	19.97400518311288	34.30305359845933	25.312254812607488	20.410686405820304
30	24.071358942454765	27.841598047218636	25.636199367565126	22.450843642761473
31	25.97084300874155	32.36750964899072	21.834259266597453	19.827388075670278
32	24.825050127200246	28.0958004105279	25.51335800727538	21.56579145499647
33	26.178088271423928	28.043295635565347	22.17068608881034	23.60793000420038
34	20.04315298108243	32.77625436879354	26.223658453466903	20.95693419665713
35	24.58015993152585	28.904770207403768	25.81233802772252	20.702731833347862
36	23.28854246744704	32.91554062086401	24.257206032699578	19.53871087898937
37	20.667266343844855	31.334057172746654	23.689758200651458	24.308918282757038
38	24.807614579288153	32.155905499330316	23.64755624945514	19.38892367192639
39	22.207538496897264	28.159400534161787	26.152331212008338	23.48072975693261
40	28.207150159693768	28.27510917030568	21.9941511662004	21.523589503800157
41	21.4023331933206	27.33953351984086	27.188359394193963	24.069773892644577
42	27.392038294803417	26.83707273001054	23.079910286180745	22.690978689005302
43	23.6576609419951	26.64805554014535	25.80976232178096	23.884521196078587
44	23.722647984212905	26.79031376060993	24.484264418009335	25.002773837167837
45	25.867022246174088	28.73497174648713	23.683616132636967	21.714389874701812
46	22.14552342307357	26.923656075892183	28.025265693974433	22.90555480705981
47	22.87444820453483	29.935250715253726	24.307729495399393	22.88257158481205
48	22.682657177501802	28.170892145285663	28.42529263982121	20.721158037391323
49	24.393322185149668	25.601922665419764	27.07463207031281	22.930123079117763
50	22.153646803350796	27.655156563295	25.13928625207047	25.051910381283733
51	21.214306659586775	26.92642991306002	28.050230228484928	23.809033198868274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4251.0
1	3389.5
2	2528.0
3	1720.0
4	912.0
5	832.0
6	752.0
7	778.5
8	805.0
9	986.0
10	1167.0
11	1219.5
12	1272.0
13	1493.0
14	1714.0
15	1682.5
16	1651.0
17	1727.0
18	1803.0
19	1805.0
20	1807.0
21	2033.5
22	2260.0
23	2309.5
24	2359.0
25	2715.5
26	3411.5
27	3751.0
28	4870.0
29	5989.0
30	6669.5
31	7350.0
32	8442.5
33	9535.0
34	10880.0
35	12225.0
36	13289.5
37	14354.0
38	14969.5
39	15585.0
40	18504.0
41	21423.0
42	25295.0
43	29167.0
44	37519.0
45	45871.0
46	47642.5
47	49414.0
48	47793.0
49	46172.0
50	44289.0
51	42406.0
52	40863.0
53	39320.0
54	36245.5
55	33171.0
56	29423.0
57	25675.0
58	22743.5
59	19812.0
60	17215.5
61	14619.0
62	13161.5
63	11704.0
64	9928.5
65	8153.0
66	7159.0
67	6165.0
68	5315.0
69	4465.0
70	3759.5
71	3054.0
72	2881.0
73	2708.0
74	2610.5
75	2272.0
76	2031.0
77	1396.5
78	762.0
79	610.0
80	458.0
81	338.5
82	219.0
83	169.5
84	120.0
85	91.5
86	63.0
87	49.5
88	36.0
89	26.0
90	16.0
91	15.5
92	15.0
93	14.5
94	14.0
95	10.5
96	7.0
97	7.0
98	7.0
99	10.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.769679277989148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65846696694538	23.41637334104268
2	8.590889347066067	5.114960411497009
3	3.01443165164383	2.6921599042456776
4	1.4618298101580849	1.740728184296398
5	0.9284539093673115	1.3819887553130037
6	0.672366063493646	1.200967324056596
7	0.5127206666087085	1.0684470862896638
8	0.4292914767740536	1.0223895664270324
9	0.32818932955156327	0.8793081974887491
>10	4.185176563844801	29.43006827975814
>50	1.0838698826080913	22.002661367688773
>100	0.1249591048382941	5.29531976806209
>500	0.006014074564409878	1.2753407353035824
>1k	0.003341152535783265	3.479287078530597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGT	3952	0.783014606234001	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC	3705	0.7340761933443758	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3617	0.7166406454322827	No Hit
AATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	3456	0.6847415180022032	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	2760	0.5468421845156484	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT	918	0.18188446571933523	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.17455361034720518	No Hit
AAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	881	0.17455361034720518	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	717	0.1420600892383043	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	650	0.1287852970779607	No Hit
AACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	636	0.12601145991012766	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT	587	0.11630302982271218	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	583	0.11551050491761704	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	558	0.1105572242607724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7150555956220924	0.0
2	0.0	0.0	0.0	0.9205176772680081	0.0
3	0.0	0.0	0.0	1.3367913836692318	0.0
4	0.0	0.0	0.0	3.4292552643466823	0.0
5	0.0	0.0	0.0	3.694552976327281	0.0
6	0.0	0.0	0.0	4.866102917284175	0.0
7	0.0	0.0	0.0	5.708755022626586	0.0
8	0.0	0.0	0.0	6.166240024092757	0.0
9	0.0	0.0	0.0	7.864026502032826	0.0
10	0.0	0.0	0.0	8.961277233137052	0.0
11	0.0	0.0	0.0	10.893651082985283	0.0
12	0.0	0.0	0.0	11.460108258902036	0.0
13	0.0	0.0	0.0	11.711933047496018	0.0
14	0.0	0.0	0.0	12.069559910920201	0.0
15	0.0	0.0	0.0	12.312270663105588	0.0
16	0.0	0.0	0.0	12.78124727569564	0.0
17	0.0	0.0	0.0	13.359196062736272	0.0
18	0.0	0.0	0.0	14.05523106063608	0.0
19	0.0	0.0	0.0	14.36966531673258	0.0
20	0.0	0.0	0.0	14.72412208053638	0.0
21	0.0	0.0	0.0	15.128507913361178	0.0
22	0.0	0.0	0.0	15.547555456930233	0.0
23	0.0	0.0	0.0	15.968386181535754	0.0
24	0.0	0.0	0.0	16.266771808304075	0.0
25	0.0	0.0	0.0	16.548316280839124	0.0
26	0.0	0.0	0.0	16.798159757170367	0.0
27	0.0	0.0	0.0	17.116754769018616	0.0
28	1.9813122627378564E-4	0.0	0.0	17.410385246356366	0.0
29	1.9813122627378564E-4	0.0	0.0	17.737896163386935	0.0
30	1.9813122627378564E-4	0.0	0.0	18.1599156753501	0.0
31	3.962624525475713E-4	0.0	0.0	18.455131202498038	0.0
32	3.962624525475713E-4	0.0	0.0	18.74321400550012	0.0
33	3.962624525475713E-4	0.0	0.0	19.041005238589623	0.0
34	3.962624525475713E-4	0.0	0.0	19.355637625912394	0.0
35	3.962624525475713E-4	0.0	0.0	19.678195262286117	0.0
36	3.962624525475713E-4	0.0	0.0	19.962909834441547	0.0
37	5.94393678821357E-4	0.0	0.0	20.259116017720856	0.0
38	5.94393678821357E-4	0.0	0.0	20.578305423247926	0.0
39	5.94393678821357E-4	0.0	0.0	21.040941836597217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAACG	35	1.2101191E-7	45.000004	1
GACGTGT	25	3.8876846E-5	45.000004	40
CGGTTCG	25	3.8876846E-5	45.000004	29
CGCATAG	35	1.2101191E-7	45.000004	1
CGGGCTC	35	1.2101191E-7	45.000004	6
GTATCGG	25	3.8876846E-5	45.000004	2
GCGATAG	35	1.2101191E-7	45.000004	1
CGTAACG	25	3.8876846E-5	45.000004	1
TTTGCGG	35	1.2101191E-7	45.000004	2
AACTCGT	25	3.8876846E-5	45.000004	41
TAAGCGA	25	3.8876846E-5	45.000004	13
TAGTACG	25	3.8876846E-5	45.000004	1
TACCCTA	20	7.029522E-4	45.0	26
ACCGGCC	20	7.029522E-4	45.0	18
GATCGCC	20	7.029522E-4	45.0	33
ACCGCGT	20	7.029522E-4	45.0	42
CGGCATT	20	7.029522E-4	45.0	33
TTATGCC	20	7.029522E-4	45.0	43
TAGCGCA	20	7.029522E-4	45.0	25
TCTTACG	20	7.029522E-4	45.0	1
>>END_MODULE