SRR3553024_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355902
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	13587	3.8176239526611253	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	12411	3.487195913481801	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	10666	2.996892402964861	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5033	1.4141533343448478	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3854	1.0828823664941474	TruSeq Adapter, Index 27 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	2980	0.8373091468999894	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	2002	0.5625144000314694	No Hit
AACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG	1733	0.48693179583143675	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTAT	1644	0.46192491191395385	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1180	0.33155194407449246	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1018	0.2860337958201977	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	947	0.2660844839309698	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	860	0.24163955246107072	No Hit
AATGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	739	0.20764142938224567	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	666	0.18713016504543384	No Hit
AGGGAGGGACATACACACACGCACACACACCCACACACAGTAATCTGTAAC	629	0.17673404476513196	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.17448623497479643	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCCTCGTTTGTCGTATGCCGT	567	0.1593135188900315	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATG	493	0.13852127832942776	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	475	0.1334637063011728	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	413	0.11604318042607235	No Hit
AATCTGTCTCTTATACCCATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	413	0.11604318042607235	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTA	358	0.1005894881175155	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	30	2.1612632E-6	45.000004	21
GTGCAGA	30	2.1612632E-6	45.000004	19
TAATACG	30	2.1612632E-6	45.000004	1
AGCAACG	30	2.1612632E-6	45.000004	1
CTCACGT	30	2.1612632E-6	45.000004	45
CTCACGA	30	2.1612632E-6	45.000004	24
ACACGAC	30	2.1612632E-6	45.000004	26
CCTAGCG	30	2.1612632E-6	45.000004	1
GCAACGG	60	0.0	45.000004	2
CACGACC	30	2.1612632E-6	45.000004	27
CGAGACA	30	2.1612632E-6	45.000004	22
CCTATGT	30	2.1612632E-6	45.000004	10
TACAGCG	30	2.1612632E-6	45.000004	1
CGTCCGG	30	2.1612632E-6	45.000004	2
GCGTAGG	30	2.1612632E-6	45.000004	2
GCTCACG	30	2.1612632E-6	45.000004	44
AACACGT	30	2.1612632E-6	45.000004	41
TGATCGG	20	7.026717E-4	45.0	2
AGGTACG	20	7.026717E-4	45.0	1
AGCCGAC	20	7.026717E-4	45.0	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content